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| 1 | Evaluation of real-time RT-PCR assays for detection and quantification of norovirus genogroups Ⅰ and Ⅱ显示文摘Noroviruses are the leading cause of acute gastroenteritis in humans. Real-time reverse transcription-polymerase chain reaction(real-time RT-PCR) is a promising molecular method for the detection of noroviruses. In this study, the performance of three Taq Man real-time RT-PCR assays was assessed, which were one commercially available real-time RT-PCR kit(assay A:Norovirus Real Time RT-PCR kit) and two in-house real-time RT-PCR assays(assay B: Light Cycler RNA Master Hybprobe and assay C: Real Time ready RNA Virus Master). Assays A and B showed higher sensitivity than assay C for norovirus GI, while they all had the same sensitivity(103 DNA copies/m L) for GII DNA standard controls. Assay B had the highest efficiency for both genogroups.No cross-reactivity was observed among GI and GII noroviruses, rotavirus, hepatitis A virus, and poliovirus. The detection rates of these assays in GI and GII norovirus-positive fecal samples were not significantly different. However, the mean quantification cycle(Cq) value of assay B for GII was lower than assays A and C with statistical significance(P-value, 0.000). All three real-time RT-PCR assays could detect a variety of noroviruses including GI.2, GII.2, GII.3, GII.4, GII.6, GII.12, GII.17,and GII.21. This study suggests assay B as a suitable assay for the detection and quantification of noroviruses GI and GII due to good analytical sensitivity and higher performance to amplify norovirus on DNA standard controls and clinical samples. | Kitwadee Rupprom Porntip Chavalitshewinkoon-Petmitr Pornphan Diraphat Leera Kittigul | 2017 | Virologica Sinica2017，32，2： | 7 |
| 2 | Annexin A1: A new immunohistological marker of cholangiocarcinoma显示文摘AIM: To evaluate a new immunohistological marker, annexin A1 (ANXA1), in cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC). METHODS: Expression of ANXA1 protein was investigated in liver tissues from patients with CCA and HCC by immunohistochemistry. Its expression on differences stages of tumor development was investigated in hamster CCA tissues induced by Opisthorchis viverrini and N -nitrosodimethylamine. Moreover, mRNA expression of ANXA1 was assessed in CCA cell lines by quantitative real-time polymerase chain reaction and silencing of ANXA1 gene expression using small interfering RNA. RESULTS: In human CCA tissue arrays, immunohistochemical analysis revealed that the positive expression of ANXA1 was 94.1% (64/68 cases) consisting of a high expression (66.2%, 45/68 cases) and a low expression (33.8%, 23/68 cases). However, expression of ANXA1 protein was negative in all histologic patterns for HCC (46/46 cases) and healthy individuals (6/6 cases). In hamster with opisthorchiasis-associated CCA, the expression of ANXA1 was observed in the cytoplasm of inflammatory cells, bile duct epithelia and tumor cells. Grading scores of ANXA1 expression were significantly increased with tumor progression. In addition, mRNA expression of ANXA1 significantly increased in all of the various CCA cell lines tested compared to an immortalized human cholangiocyte cell line (MMNK1). Suppressing the ANXA1 gene significantly reduced the matrix metalloproteinase (MMP) 2 and MMP9, and transforming growth factor-β genes, but increased nuclear factor-kB gene expression. CONCLUSION: ANXA1 is highly expressed in CCA, but low in HCC, suggesting it may serve as a new immunohistochemical marker of CCA. ANXA1 may play a role in opisthorchiasis-associated cholangiocarcinogenesis. | Nuttanan Hongsrichan Rucksak Rucksaken Yaovalux Chamgramol Porntip Pinlaor Anchalee Techasen Puangrat Yongvanit Narong Khuntikeo Chawalit Pairojkul Somchai Pinlaorr | 2013 | World Journal of Gastroenterology2013，19，16： | 4 |
| 3 | Ultrafiltration enhanced by coagulation in an immersed membrane system显示文摘 | Porntip Choksuchart | 2002 | Desalination2002，145，： | 1 |
| 4 | Characterization of associated proteins and phospholipids in natural rubber latex显示文摘 | Jitlada Sansatsadeeku Jitladda Sakdapipanich and Porntip Rojruthai | 2011 | Journal of Bioscience and Bioengineering2011，111，6： | 1 |
| 5 | Linear transgene constructs lacking vector backbone sequences generate transgenic rice plants which accumulate higher levels of proteins conferring insect resistance显示文摘 | Nguyen Thi Loc Porntip Tinjuangjun Angharad M.R. Gatehouse Paul Christou John A. Gatehouse | 2002 | Molecular Breeding2002，，4： | 1 |
| 6 | Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns显示文摘 | Xiangdong Fu Le Tan Duc Stefania Fontana Bui Ba Bong Porntip Tinjuangjun Durailagaraja Sudhakar Richard M. Twyman Paul Christou Ajay Kohli | 2000 | Transgenic Research2000，，1： | 1 |
| 7 | Ultrafiltration enhanced by coagulation in an immersed membrane system显示文摘 | Porntip Choksuchart Marc Heran Alain Grasmick | 2002 | Desalination2002，145，： | 1 |
| 8 | Linear transgene constructs lacking vector backbone sequences generate transgenic rice plants which accumulate higher levels of proteins conferring insect resistance显示文摘 | Nguyen Thi Loc Porntip Tinjuangjun Angharad M.R. Gatehouse Paul Christou John A. Gatehouse | 2002 | Molecular Breeding2002，，4： | 1 |
| 9 | Diversity of type I polyketide synthase genes in the wood-decay fungus Xylaria sp. BCC 1067显示文摘 | Alongkorn Amnuaykanjanasin Juntira Punya Porntip Paungmoung Amporn Rungrod Anuwat Tachaleat Somchai Pongpattanakitshote Supapon Cheevadhanarak Morakot Tanticharoen | 2005 | FEMS Microbiology Letters2005，，1： | 1 |
| 10 | 查看详情显示文摘 | Worapon K Porntip W | | 0，，05： | 1 |
| 11 | Benefits of MBR in seafood wastewater treatment and water reuse: study case in Southern part of Thailand 显示文摘 | Porntip Choksuchart Sridanga Jansongkod Kaimanb Anthony Pottierc | | Desalination0，，200： | 1 |
| 12 | Formation, Physical Stability and In Vitro Antimalarial Activity of Dihydroartemisinin Nanosuspensions Obtained by Co-grinding Method显示文摘 | Jiraporn Chingunpitak Satit Puttipipatkhachorn Porntip Chavalitshewinkoon-Petmitr Yuichi Tozuka Kunikazu Moribe Keiji Yamamoto | | Drug Development and Industrial Pharmacy0，，： | 1 |
| 13 | Performance and microbial surveying in submerged membrane bioreactor for seafood processing wastewater treatment显示文摘 | PORNTIP C S ANTHONY P CHRISTELLE W | 2008 | Journal of Membrane Science2008，317，12： | 1 |
| 14 | Life cycle assessment of bagasse waste management options 显示文摘 | Worapon K Porntip W | 2009 | Waste Manage2009，29，5： | 1 |
| 15 | Particle-bombardment-mediated co-transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight and sap-sucking insect pests显示文摘 | Kexuan Tang Porntip Tinjuangjun Yanan Xu Xiaofen Sun John A. Gatehouse Pamela C. Ronald Huaxiong Qi Xinggui Lu Paul Christou Ajay Kohli | 1999 | Planta1999，，4： | 1 |
| 16 | Efficacy of Acupuncture versus Combined Oral Contraceptive Pill in Treatment of Moderate-to-Severe Dysmenorrhea: A Randomized Controlled Trial显示文摘 | Intira Sriprasert Suparerk Suerungruang Porntip Athilarp | 2015 | Evidence-Based Complementary and Alternative Medicine2015，4，： | 1 |
| 17 | Curcumin induces a nuclear factor-erythroid 2-related factor 2-driven response against oxidative and nitrative stress after praziquantel treatment in liver fluke-infected hamsters显示文摘 | Lakhanawan Charoensuk Porntip Pinlaor Suksanti Prakobwong Yusuke Hiraku Umawadee Laothong Wipaporn Ruangjirachuporn Puangrat Yongvanit Somchai Pinlaor | 2011 | International Journal for Parasitology2011，，6： | 1 |
| 18 | Particle-bombardment-mediated co-transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight and sap-sucking insect pests显示文摘 | Kexuan Tang Porntip Tinjuangjun Yanan Xu Xiaofen Sun John A. Gatehouse Pamela C. Ronald Huaxiong Qi Xinggui Lu Paul Christou Ajay Kohli | 1999 | Planta1999，，4： | 1 |
| 19 | Application of Sulphatoethylsulphone Reactive Disperse Dye on PLA and Cotton Fabrics 显示文摘 | PIMONRAT H PORNTIP S | 2014 | Applied Mechanics and Materials2014，535，： | 1 |
| 20 | Stability study of resveratrol-loaded emulsions using pectin as an emulsifier显示文摘Phenolic compound such as resveratrol has been suggested to prevent cancer and heart diseases because of its anti-oxidant and anti-inflammatory activities[1].However,therapeutic application of resveratrol is quite limited due to its poor solubility,low bioavailability,photosensitive and auto-oxidation properties[2].The aim of this study was to prepare resveratrolloaded emulsions using pectin as an emulsifier.The emulsions should be stable for up to 7 days,before further manufacturing processes. | Porntip Benjasirimongkol Pornsak Sriamornsak | 2016 | Asian Journal of Pharmaceutical Sciences2016，11，1： | 0 |
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