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您的检索式：作者名="Yoshihide Ueda"
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| 1 | Decrease in alpha-fetoprotein levels predicts reduced incidence of hepatocellular carcinoma in patients with hepatitis C virus infection receiving interferon therapy: a single center study显示文摘 | Yukio Osaki Yoshihide Ueda Hiroyuki Marusawa Jun Nakajima Toru Kimura Ryuichi Kita Hiroki Nishikawa Sumio Saito Shinichiro Henmi Azusa Sakamoto Yuji Eso Tsutomu Chiba | 2012 | Journal of Gastroenterology2012，，4： | 3 |
| 2 | Exploring the hepatitis C virus genome using single molecule realtime sequencing显示文摘Single molecular real-time(SMRT)sequencing,also called third-generation sequencing,is a novel sequencing technique capable of generating extremely long contiguous sequence reads.While conventional short-read sequencing cannot evaluate the linkage of nucleotide substitutions distant from one another,SMRT sequencing can directly demonstrate linkage of nucleotide changes over a span of more than 20 kbp,and thus can be applied to directly examine the haplotypes of viruses or bacteria whose genome structures are changing in real time.In addition,an error correction method(circular consensus sequencing)has been established and repeated sequencing of a single-molecule DNA template can result in extremely high accuracy.The advantages of long read sequencing enable accurate determination of the haplotypes of individual viral clones.SMRT sequencing has been applied in various studies of viral genomes including determination of the full-length contiguous genome sequence of hepatitis C virus(HCV),targeted deep sequencing of the HCV NS5A gene,and assessment of heterogeneity among viral populations.Recently,the emergence of multi-drug resistant HCV viruses has become a significant clinical issue and has been also demonstrated using SMRT sequencing.In this review,we introduce the novel third-generation PacBio RSII/Sequel systems,compare them with conventional next-generation sequencers,and summarize previous studies in which SMRT sequencing technology has been applied for HCV genome analysis.We also refer to another long-read sequencing platform,nanopore sequencing technology,and discuss the advantages,limitations and future perspectives in using these thirdgeneration sequencers for HCV genome analysis. | Haruhiko Takeda Taiki Yamashita Yoshihide Ueda Akihiro Sekine | 2019 | World Journal of Gastroenterology2019，25，32： | 2 |
| 3 | Pancreatic cancer complicated by disseminated intravascular coagulation associated with production of tissue factor显示文摘 | Chisato Ueda Yoshihide Hirohata Yasuyuki Kihara Hayato Nakamura Shintaro Abe Kazuhisa Akahane Kohji Okamoto Hideaki Itoh Makoto Otsuki | 2001 | Journal of Gastroenterology2001，，12： | 1 |
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