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| 1 | An algorithm for automatic identification of multiple developmental stages of rice spikes based on improved Faster R-CNN显示文摘Spike development directly affects the yield and quality of rice. We describe an algorithm for automatically identifying multiple developmental stages of rice spikes(AI-MDSRS) that transforms the automatic identification of multiple developmental stages of rice spikes into the detection of rice spikes of diverse maturity levels. The scales vary greatly in different growth and development stages because rice spikes are dense and small, posing challenges for their effective and accurate detection. We describe a rice spike detection model based on an improved faster regions with convolutional neural network(Faster R-CNN).The model incorporates the following optimization strategies: first, Inception_Res Net-v2 replaces VGG16 as a feature extraction network;second, a feature pyramid network(FPN) replaces single-scale feature maps to fuse with region proposal network(RPN);third, region of interest(Ro I) alignment replaces Ro I pooling, and distance-intersection over union(DIo U) is used as a standard for non-maximum suppression(NMS). The performance of the proposed model was compared with that of the original Faster R-CNN and YOLOv4 models. The mean average precision(m AP) of the rice spike detection model was92.47%, a substantial improvement on the original Faster R-CNN model(with 40.96% m AP) and 3.4%higher than that of the YOLOv4 model, experimentally indicating that the model is more accurate and reliable. The identification results of the model for the heading–flowering, milky maturity, and full maturity stages were within two days of the results of manual observation, fully meeting the needs of agricultural activities. | Yuanqin Zhang Deqin Xiao Youfu Liu Huilin Wu | 2022 | The Crop Journal2022，10，5： | 4 |
| 2 | Experimental Research on Structural Behaviors of Glulam I-Beam with a Special-Shaped Section显示文摘In order to enhance the bearing capacity of structural components,save materials,and reduce cost,a glued laminated timber(glulam)I-beam that is theoretically suitable for engineering application was proposed.In this study,18 glulam specimens were fabricated using larch dimension lumber and resorcinol-formaldehyde resin.Four-point bending tests were carried out to compare the ultimate bearing capacity,strain,and deflection of various specimens.The results showed that:(1)The typical failure mode at bending is the web shear failure parallel to grain.Before the failure,cracks and sounds appear at the beam web,which represent the sudden brittle failure.(2)The cross-sectional strain of glulam beam changed linearly with the beam height,indicating that the plane section assumption was basically established.(3)Stiffener could improve the initial flexural stiffness of glulam beam,which experiences an increase of 28.21%.Larger the shear span ratio,smaller the initial flexural stiffness.The initial flexural stiffness improves by 10-23.5%with the increase in the thickness of the lower flange.(4)The effects of stiffener and shear-span ratio on shear strength are relatively significant.After the stiffeners are set at the support and the loading point in pairs,the shear strength of the glulam beam increases by 15.05%averagely.With the increase in the shear-span ratio,the shearing strength of the glulam I-beam gradually reduces.The equation of the shearing strength with the shear span ratio is obtained,which is shown by high fitting precision.(5)The shear strength correlation,as proposed by Soltis and Rammer,is suitable not only for rectangular beams,but also for glulam I-beams. | Ruyuan Yang Chaokun Hong Xiaofeng Zhang Quan Yuan Youfu Sun | 2020 | Journal of Renewable Materials2020，8，2： | 2 |
| 3 | Some new data on the genesis of the Linghou Cu–Pb–Zn polymetallic deposit—Based on the study of fluid inclusions and C–H–O–S–Pb isotopes显示文摘 | Yanwen Tang Xiaofeng Li Xiaoqi Zhang Jianling Yang Yuling Xie Lantingguang Youfu Huang Cheng Huang Rongchao Yin | 2015 | Ore Geology Reviews2015，，： | 1 |
| 4 | A hierarchical model incorporating segmented regions and pixel descriptors for video background subtraction显示文摘 | Chen Shengyong Zhang Jianhua Li Youfu | 2012 | IEEE Transactions on Industrial Informatics2012，8，1： | 1 |
| 5 | TaGW2L,a GW2-like RING finger E3 ligase,positively regulates heading date in common wheat(Triticum aestivum L.)显示文摘RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate degradation.In this study,we identified TaGW2L,encoding a Grain width and weight2(GW2)-like RING finger E3 ligase,as a novel positive regulator of heading date in wheat(Triticum aestivum L.).TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs,particularly in the conserved RING finger domain.Expression analysis indicated that TaGW2L was constitutively expressed in various wheat tissues.TaGW2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2_(s).An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to TaGW2.Overexpression of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day conditions.Transcriptome analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type control.Together,our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops. | Daijing Zhang Xiaoxu Zhang Wu Xu Tingting Hu Jianhui Ma Youfu Zhang Jian Hou Chenyang Hao Xueyong Zhang Tian Li | 2022 | The Crop Journal2022，10，4： | 1 |
| 6 | Mechanical compression upregulates MMP9 through SMAD3 but not SMAD2 modulation in hypertrophic scar fibroblasts显示文摘 | Dong Huang Yingping Liu Yongjun Huang Youfu Xie Kuanhong Shen Dawei Zhang Yong Mou | 2014 | Connective Tissue Research . 2014 (5-6)2014，，： | 1 |
| 7 | TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity显示文摘Drought is a major environmental stress limiting global wheat(Triticum aestivum)production.Exploring drought tolerance genes is important for improving drought adaptation in this crop.Here,we cloned and characterized TaTIP41,a novel drought tolerance gene in wheat.TaTIP41 is a putative conserved component of target of rapamycin(TOR)signaling,and the Ta TIP41 homoeologs were expressed in response to drought stress and abscisic acid(ABA).The overexpression of Ta TIP41 enhanced drought tolerance and the ABA response,including ABA-induced stomatal closure,while its downregulation using RNA interference(RNAi)had the opposite effect.Furthermore,Ta TIP41 physically interacted with TaTAP46,another conserved component of TOR signaling.Like TaTIP41,TaTAP46 positively regulated drought tolerance.Furthermore,TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase(PP2A)catalytic subunits,such as TaPP2A-2,and inhibited their enzymatic activities.Silencing TaPP2A-2 improved drought tolerance in wheat.Together,our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat,and their potential application in improving wheat environmental adaptability. | Jianhui Ma Yuke Geng Hong Liu Mengqi Zhang Shujuan Liu Chenyang Hao Jian Hou Youfu Zhang Daijing Zhang Weijun Zhang Xueyong Zhang Tian Li | 2023 | Journal of Integrative Plant Biology2023，65，9： | 0 |
| 8 | Seasonal variation of foliar δ^(13)C values and its indictor significance in Sabina przewalskii and Sabina chinensis显示文摘Seasonal variation of foliar δ13C values as well as proline,silicon,MDA and relative water content in Sabina przewalskii Kom.and S.chinensis (Lin.) Ant.were measured.The results show that foliar δ13C values were significantly different at different seasons:lower in winter but higher in summer.δ13C values were positively correlated with air temperature and soil temperature.This demonstrates that foliar δ13C of Sabina is a successful empirical indictor of temperature.Furthermore,foliar δ13C values were positively related to proline and silicon content,and negatively related to relative water content and MDA content.Compared with S.chinensis,S.przewalskii has higher δ13C values,proline and silicon content as well as lower MDA and relative water content.All these results provided strong evidence that it is feasible for δ13C to be regarded as another index to evaluate freezing tolerance of Sabina. | Tuo Chen HuiJuan Pei YouFu Zhang LongYing Wen HongGuan Tang YuanQing He | 2011 | Research in Cold and Arid Regions2011，3，2： | 0 |
| 9 | Foliar carbohydrate differs between Picea crassifolia and Sabina przewalskii with the altitudinal variation of Qilian Mountains, China显示文摘Nonstructural carbohydrates(NSC) and nitrogen metabolism strongly influence growth and development in plants. The biosynthesis of cellulose and lignin(structural carbohydrates, SC) depends largely on the supply of NSC. We desire to examine which hypothesis, carbon limitation or growth limitation, best fits the alpine plant response between NSC, SC, carbon(C), nitrogen(N) and altitude. We compared the foliar concentrations of carbohydrates, C and N between the leaves of Picea crassifolia(lower-elevation tree-line species) and Sabina przewalskii(high-elevation tree-line species) in their response to changing elevation. Our site was located in the mid-northern area of Qilian Mountains, China. We found that foliar soluble sugar(SG) concentrations were significantly higher in P. crassifolia than in S. przewalskii at the 2,700–3,400 m level. Foliar NSC levels in P. crassifolia increased at the 2,700–3,100 m level, indicating that growth was limited gradually resulting in a surplus of NSC(to conform to GLH), subsequently decreasing at the 3,100–3,400 m level, the assimilation declined leading to C deficit(to conform to CLH). SC(SC metabolism disorders at 3,100–3,400 m), C, N and starch were significantly lower in P. crassifolia than in S. przewalskii. Conversely, foliar SG concentration shows a fall-rise trend with increasing elevation for S. przewalskii. SC concentration in S. przewalskii leaves decreased with an increase of elevation and has a significantly positive correlation to N concentration marking the assimilation of plants. Therefore, the high-elevation tree-line species(S. przewalskii) utilize or store more foliar SG leading to a decrease of SG concentration for survival and growth/regrowth in an adverse environment, higher total C, N, SC, starch contents and lower NSC level. Also, their change trends along the elevational gradient in leaves of S. przewalskii indicate better assimilation strategies for SG use under environmental stress compared to P. crassifolia. This indicates that foliar C metabolism along the elevation follows the principle of the growth-limitation hypothesis(GLH) or carbon limitation hypothesis(CLH), which depends on the acclimation of different alpine life-forms to the environment. | ManXiao Zhang HuiJuan Pei YouFu Zhang Tuo Chen GuangXiu Liu | 2015 | Research in Cold and Arid Regions2015，7，2： | 0 |
| 10 | Next-generation Sequencing of MHC Class Ⅰ Genes Reveals Trans-species Polymorphism in Eutropis multifasciata and Other Species of Scincidae显示文摘The genes of the major histocompatibility complex(MHC) encode cell surface proteins that are essential for adaptive immunity. MHC genes show the most prominent genetic diversity in vertebrates,reflecting the adaptation of populations to their evolving environment, population survival and reproduction. In the present study, we used nextgeneration sequencing(NGS) to study the loci polymorphism of exon 3 of the MHC class Ⅰ genes in an ovoviviparous skink, the many-lined sun skink,Eutropis multifasciata and five other species of Scincidae, to quantify genetic variation. In addition,we genotyped the same MHC class Ⅰ genes of E.multifasciata using clone sequencing, to directly compare the effectiveness of both analytical techniques for MHC genotyping. NGS detected 20MHC class Ⅰ alleles in E. multifasciata, and 2 to 15 alleles in the other five Scincidae species. However,clone sequencing detected only 15 of those MHC class Ⅰ alleles in E. multifasciata. In addition, transspecies polymorphism of MHC class Ⅰ genes was studied by constructing a phylogenetic tree using the gene sequences obtained by NGS. Phylogenetic analysis revealed that MHC class I alleles were shared among different species of Scincidae with trans-species polymorphism, and did not exhibit specific genealogical inheritance. These results have important implications for understanding polymorphism interspecies diversity in the MHC genes of Scincidae, and the evolution of the MHC more broadly. | Shufang ZHANG Youfu LIN Yingzhi CHENG Haiyun YANG Xiaming ZHU Yu DU Longhui LIN Yanfu QU LianCHEN Hong LI | 2023 | Asian Herpetological Research2023，14，4： | 0 |
| 11 | Analytical Investigation into the Rotational Performance of Glulam Bolted Beam-Column Connections under Coupled Bending Moment and Shear Force显示文摘Considering the glulam beam-column connection form and the number of bolts,monotonic loading test and finite element analysis was carried out on 9 connection specimens in 3 groups to study the rotational performance and failure mode of the connection.The test results revealed that compared with U-shaped connectors,T-shaped connectors can effectively improve the ductility of connections,and the increase in the number of bolts can reduce the initial stiffness and ductility of connections.By theoretical analysis,formulas for calculating the initial stiffness and ultimate moment of connections were deduced.Subsequently,the moment-rotation theoretical model of connections was established based on the formulas,which were validated according to the test data and simulation results.The proposed model can not only improve the current theoretical system of heavy-duty glulam beam-column structure but also provide a theoretical basis for calculating the mechanical properties of the glulam beam-column connection. | Xiaofeng Zhang Lisheng Luo Youfu Sun Xinyue Cui Yongqiang Zhang | 2023 | Journal of Renewable Materials2023，11，4： | 0 |
| 12 | Formulation of Classical Swine Fever Immunization Procedures in Large scale Pig Farms显示文摘[Objective] The paper was to formulate classical swine fever( CSF) immunization procedures in large-scale pig farms. [Method] Antibody maintenance time and dynamics of material antibody was detected by enzyme-linked immunosorbent assay( ELISA),and scientific primary immunization time of piglets was explored. [Result] The positive rate of sows reached the highest level of 93% at 4 weeks post immunization. Piglets were immunized at 9 weeks of age,the antibody level did not increase at 3 weeks post immunization,but increased rapidly after secondary immunization; the antibody level in late fattening period was high,and pigs could be protected completely. [Conclusion]General prevention of CSF is suitable for scale farming. It takes about 4 weeks to produce CSF antibody. Therefore,pig farms should conduct immunization timely at one month prior to susceptible stage according to their infection pressure. | He Junchang Zhang Jianyuan Xie Youfu Fan Lizhe Gao Shihua | 2017 | Animal Husbandry and Feed Science2017，9，5： | 0 |
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