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| 1 | PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells(HDDPCs) and HDDPC-derived iPS cells显示文摘The ability of human deciduous tooth dental pulp cells(HDDPCs) to differentiate into odontoblasts that generate mineralized tissue holds immense potential for therapeutic use in the field of tooth regenerative medicine. Realization of this potential depends on efficient and optimized protocols for the genetic manipulation of HDDPCs. In this study, we demonstrate the use of a Piggy Bac(PB)-based gene transfer system as a method for introducing nonviral transposon DNA into HDDPCs and HDDPC-derived inducible pluripotent stem cells. The transfection efficiency of the PB-based system was significantly greater than previously reported for electroporation-based transfection of plasmid DNA. Using the neomycin resistance gene as a selection marker, HDDPCs were stably transfected at a rate nearly 40-fold higher than that achieved using conventional methods. Using this system, it was also possible to introduce two constructs simultaneously into a single cell. The resulting stable transfectants, expressing td Tomato and enhanced green fluorescent protein, exhibited both red and green fluorescence. The established cell line did not lose the acquired phenotype over three months of culture. Based on our results, we concluded that PB is superior to currently available methods for introducing plasmid DNA into HDDPCs. There may be significant challenges in the direct clinical application of this method for human dental tissue engineering due to safety risks and ethical concerns. However, the high level of transfection achieved with PB may have significant advantages in basic scientific research for dental tissue engineering applications, such as functional studies of genes and proteins. Furthermore, it is a useful tool for the isolation of genetically engineered HDDPC-derived stem cells for studies in tooth regenerative medicine. | Emi Inada Issei Saitoh Satoshi Watanabe Reiji Aoki Hiromi Miura Masato Ohtsuka Tomoya Murakami Tadashi Sawami Youichi Yamasaki Masahiro Sato | 2015 | International Journal of Oral Science2015，7，3： | 2 |
| 2 | Development of High Strength Steel Designed for Carbonitriding with High Nitrogen Content to Be Used for Automatic Transmission Gears显示文摘To downsize and lighten automatic transmission components, the gears installed must be strengthened in terms of pitting endurance and/or wear resistance. The most important metallurgical factor affecting fractures is well known to be resistance to softening when steel is tempered at approximately 573 K. Carbonitriding with a high amount of nitrogen is a very effective production technique because nitrogen increases the resistance during tempering. However, structural anomalies begin to appear in the surface layer when the nitrogen content exceeds 0.6 mass% in the chromium steel generally used. To address this, we have developed new high-strength chromium steel with an optimized chemical composition that effectively inhibits anomalies even when Carbonitriding with a nitrogen content of more than 0.6 mass%. We performed a drivetrain durability test on an automatic transmission component designed to have excellent contact fatigue strength and a tooth root bending impact and fatigue strength. We found that the developed steel that was carbonitrided with a content of about 0.9 mass%, and then shot peened hard, has a pitting life of roughly 4.5 times that of conventionally manufactured steel. | Youichi Watanabe | 2004 | 材料热处理学报2004，25，5： | 2 |
| 3 | Transmural dispersion of repolarization and ventricular tachyarrhythmias显示文摘 | Norikazu Watanabe Youichi Kobayashi Kaoru Tanno Fumito Miyoshi Taku Asano Mitsuharu Kawamura Yoshino Mikami Tarou Adachi Syunsyou Ryu Akira Miyata Takashi Katagiri | 2004 | Journal of Electrocardiology2004，，3： | 1 |
| 4 | Heterologous expression of Zygosaccharomyces rouxii glycerol 3- phosphate dehydrogenase gene (Zrgpdl) and glycerol dehydrogenase gene (ZrGCY1) in Saccharomyces cerevisiae显示文摘 | Yasuo Watanabe Syoko Tsuchimoto Youichi Tamai | 2004 | FEMS Yeast Research2004，，4： | 1 |
| 5 | Evaluation of Important Factors Affecting Quench Distortion of Carburized Hypoid Gear with Shaft by Using Computer Simulation Methods显示文摘To minimize quenching distortion and dispersion, carburizing and quenching process conditions must be optimized; this includes the parts racking design used for quenching. We investigated some factors affecting carburized quenching distortion with an experiment using a hypoid gear having a shaft and with numerical simulation methods. The experimental results and those obtained from simulation were generally in agreement. Focusing on the surface temperature distribution in the gear, we studied quenching distortion characteristics in terms of changes in tooth profile and helix deviation. In our experiments, distortions occur during quenching in 373 K oil after austenitized temperature treatments conducted with various attitudes. We calculated the distortions by simulating the carburized oil-quenching process for the hypoid gear. Our results show large differences between the cooling rates of the tooth toe, middle section, and heel edges, and these greatly influence the change in tooth profile and helix deviation. We found that reducing the differences in temperatures on the gear surfaces during quenching is most important for minimizing the quench distortion of the hypoid gear. | Tsuyoshi Sugimoto Youichi Watanabe | 2004 | 材料热处理学报2004，25，5： | 0 |
| 6 | 用计算机模拟法对渗碳的准双曲面主动锥齿轮淬火变形的关键因素的评定显示文摘为使淬火变形和偏差减至最小.渗碳和淬火工艺必须最佳化。这当中已包括零部件用于淬火的挂装设计。作者以准双曲面主动齿轮为例,用数字模拟方法对一些影响渗碳淬火变形的因素进行了研究和试验。试验结果与模拟方法获得的结果是一致的。重点是对齿轮表面温度分布的研讨,作者根据齿形的变化和螺旋线偏差研究了淬火变形特性。在试验中,以各种状态的处理温度奥氏体化后,在100℃的油中淬火时均出现变形。作者用准双曲面齿轮通过模拟渗碳油淬过程来预测变形。结果表明,在齿顶、齿中部和齿根边缘的冷却率间差异很大,而这些又很大程度上影响着齿形和螺旋线偏差的变化。作者发现在淬火期闻,降低齿轮表面的温差.对于降低准双曲面齿轮的淬火变形是极为重要的。 | Tsuyoshi Sugimoto Youichi Watanabe 翁远松(译) 赵世鑫(校) | 2006 | 綦齿传动2006，，2： | 0 |
| 7 | Development of Ferroelectric RAM (FRAM) for Mass Production显示文摘 | Takashi Eshita Wensheng Wang Kou Nakamura Souichirou Ozawa Youichi Okita Satoru Mihara Yukinobu Hikosaka Hitoshi Saito Junichi Watanabe Ken'ichi Inoue Hideshi Yamaguchi KenjiNomura | 2015 | Journal of Physical Science and Application2015，5，1： | 0 |
| 8 | 用于自动变速齿轮高氮势碳氮共渗高强度钢的研制显示文摘为使自动变速零件小型化和轻量化。必须在抗点蚀和,或耐磨方面强化齿轮。影响破断的最重要的冶金因素是钢在约573K温度回火时的抗软化能力。采用高氮势进行碳氮共渗是一种非常有效的制造技术,因为氮提高抗回火性。但是,在通常使用的含Cr钢中氮含量超过0.6%时开始在表面出现异常组织。为此,我们开发研究了一种新的高强度Cr钢,即使氮含量超过0.6%,钢中最佳的化学成分可有效地阻止异常组织的出现。传动系统耐久疲劳试验表明:自动变速零件具有优良的接触疲劳强度和齿根弯曲冲击、疲劳强度;该钢在约0.9%的氮势下碳氮共渗后再喷九处理,其点蚀寿命比传统钢高约4.5倍。 | Youichi Watanabe 胡明霞(译) 赵世鑫(校) | 2005 | 綦齿传动2005，，2： | 0 |
| 9 | Development of New Sliver Probe for Cooling Power Test of Polymer Quenchants显示文摘Working group on cooling power test of quenchants of Japanese Heat Treatment Society has studied on the new method for testing cooling power of aqueous quenchants. This method employed the cooling curve test of a new sliver rod probe, which has a metal-sheathed thermocouple at its geometrical center. The experimental study has clarified that the new silver probe has more excellent durability than the JIS silver probe employed for cooling power test of heat treating oils (JIS K 2242) and an aluminum alloy probe. Good repeatability and reproducibility of the cooling curves during polymer quenching have been confirmed. This new method is useful especially for cooling power test of aqueous quenchants used for solution heat treatment of aluminum alloys. This new silver rod probe is expected to be adopted for the revised version of JIS K 2242 as the cooling power test method for aqueous quenchants. | Michiharu NARAZAKI Mamoru TAJIMA Shigeru ASADA Katsumi ICHITANI Hideo YOKOTA Mitsugu TANAKA Youichi WATANABE | 2004 | 材料热处理学报2004，25，5： | 0 |
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