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| 1 | Regulation of Actin Dynamics in Pollen Tubes:Control of Actin Polymer Level显示文摘Actin cytoskeleton undergoes rapid reorganization in response to internal and external cues. How the dynamics of actin cytoskeleton are regulated, and how its dynamics relate to its function are fundamental questions in plant cell biology. The pollen tube is a well characterized actin-based cell morphogenesis in plants. One of the striking features of actin cytoskeleton characterized in the pollen tube is its surprisingly low level of actin polymer. This special phenomenon might relate to the function of actin cytoskeleton in pollen tubes. Understanding the molecular mechanism underlying this special phenomenon requires careful analysis of actin-binding proteins that modulate actin dynamics directly. Recent biochemical and biophysical analyses of several highly conserved plant actin-binding proteins reveal unusual and un- expected properties, which emphasizes the importance of carefully analyzing their action mechanism and cellular activity. In this review, we highlight an actin monomer sequestering protein, a barbed end capping protein and an F-actin severing and dynamizing protein in plant. We propose that these proteins function in harmony to regulate actin dynamics and maintain the low level of actin polymer in pollen tubes. | Naizhi Chen Xiaolu Qu Youjun Wu Shanjin Huang | 2009 | Journal of Integrative Plant Biology2009，51，8： | 9 |
| 2 | Comparison of QTLs for rice seedling morphology under different water supply conditions显示文摘The variation of seedling characteristics under different water supply conditions is strongly associated with drought resistance in rice (Oryza sativa L.) and a better elucidation of its genetics is helpful for improving rice drought resistance. Ninety-six doubled-haploid (DH) rice lines of an indica and japonica cross were grown in both flooding and upland conditions and QTLs for morphological traits at seedling stage were examined using 208 restriction fragment length polymorphism (RFLP) and 76 microsatellite (SSR) markers. A total of 32 putative QTLs were associated with the four seedling traits: average of three adventitious root lengths (ARL), shoot height (SH), shoot biomass (SW), and root to shoot dry weight ratio (RSR). Five QTLs detected were the same under control and upland conditions. The ratio between the mean value of the seedling trait under upland and flooding conditions was used for assessing drought tolerance. A total of six QTLs for drought tolerance were detected. Comparative analysis was performed for the QTLs detected in this case and those reported from two other populations with the same upland rice variety Azucena as parent. Several identical QTLs for seedling elongation across the three populations with the positive alleles from the upland rice Azucena were detected, which suggests that the alleles of Azucena might be involved in water stress-accelerated elongation of rice under different genetic backgrounds. Five cell wall-related candidate genes for OsEXP1, OsEXP2, OsEXP4, EXT, and EGase were mapped on the intervals carrying the QTLs for seedling traits. | Bingsong Zheng Ling Yang Chuanzao Mao Youjun Huang Ping Wu | 2008 | Journal of Genetics and Genomics2008，35，8： | 7 |
| 3 | Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube Growth显示文摘花粉试管生长在 flowering 植物复制期间是必要的步,其生长取决于动态顶端的肌动朊细丝的一张人口。顶端的肌动朊细丝被认为涉及泡熔化的规定并且在花粉试管指向。然而,在花粉试管调整顶端的肌动朊结构的构造的分子的机制仍然保持大部分不清楚。这里,我们在花粉试管在顶端的膜在肌动朊聚合的规定作为一个重要播放器识别 profilin。profilin 的 Downregulation 减少了在花粉试管的细丝状的肌动朊的数量和顶端的肌动朊细丝的导致的组织的破坏。肌动朊动力学的直接可视化表明在顶端的膜发源的肌动朊细丝的延伸在 profilin 异种花粉试管减少了。仅仅在有约束力的 poly-L-proline 是有缺点的变异的 profilin 部分在 profilin 异种花粉试管救肌动朊聚合缺点,尽管它充分救肌动朊周转显型。我们建议 profilin 在花粉试管尖端控制肌动朊结构的构造,大概由在顶端的膜赞成调停 formin 的肌动朊聚合。 | Xiaonan Liu Xiaolu Qu Yuxiang Jiang Ming Chang Ruihui Zhang Youjun Wu Ying Fu Shanjin Huang | 2015 | Molecular Plant2015，8，12： | 6 |
| 4 | Effects of soil nutrients and climate factors on belowground biomass in an alpine meadow in the source region of the Yangtze-Yellow rivers, Tibetan Plateau of China显示文摘Improving our knowledge of the effects of environmental factors(e.g.soil conditions,precipitation and temperature) on belowground biomass in an alpine grassland is essential for understanding the consequences of carbon storage in this biome.The object of this study is to investigate the relative importance of soil nutrients and climate factors on belowground biomass in an alpine meadow in the source region of the Yangtze and Yellow rivers,Tibetan Plateau.Soil organic carbon(SOC),total nitrogen(TN) and total phosphorous(TP) contents and belowground biomass were measured at 22 sampling sites across an alpine meadow on the Tibetan Plateau.We analyzed the data by using the redundancy analysis to determine the main environmental factors affecting the belowground biomass and the contribution of each factor.The results showed that SOC,TN and TP were the main factors that influenced belowground biomass,and the contribution of SOC,TN and TP on biomass was in the range of 47.87%–72.06% at soil depths of 0–30 cm.Moreover,the combined contribution of annual mean temperature(AMT) and mean annual precipitation(MAP) on belowground biomass ranged from 0.92% to 4.10%.A potential mechanism for the differences in belowground biomass was caused by the variations in soil nitrogen and phosphorous,which were coupled with SOC.A significant correlation was observed between MAP and soil nutrients(SOC,TN and TP) at the soil depth of 0–10 cm(P<0.05).We concluded that precipitation is an important driving force in regulating ecosystem functioning as reflected in variations of soil nutrients(SOC,TN and TP) and dynamics of belowground biomass in alpine grassland ecosystems. | WANG Haiming SUN Jian LI Weipeng WU Jianbo CHEN Youjun LIU Wenhui | 2016 | Journal of Arid Land2016，8，6： | 5 |
| 5 | Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats显示文摘Objective To investigate the expression variation of RAR‐β2, RASSF1A, and CDKN2A gene in the process of nickel‐induced carcinogenesis. Methods Nickel subsulfide (Ni 3 S 2 ) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real‐time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real‐time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR‐β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure. | ZHANG Jun ZHANG Jing LI MengJie Wu YouJun FAN YingYing ZHOU Yang TAN LiLi SHAO ZhiHua SHI HongJun | 2011 | Biomedical and Environmental Sciences2011，24，2： | 4 |
| 6 | Genomic insights into the ESBL and MCR-l-producing ST648 Escherichia coli with multi-drug resistance显示文摘多粘菌素由 multidrug 抵抗的克否定的病原体对严重感染充当避难处的一根最终的线。这个常规想法被活动 colistin 抵抗基因(mcr-1 ) 的最近的发现戏剧性地质问在食物动物和世界范围的人是流行的。更重要地, mcr-1 基因被发现与另外的抗菌素抵抗基因 co 局部性,提起可能性与平底锅药抵抗超级臭虫正在出现。然而,很少在 mcr-1-positive 的染色体上被报导细菌的主人水库。这里,我们报导三个人定序的染色体 mcr-1-positive Escherichia coli (E15004, E15015 和 E15017 ) 孤立并且通过细菌的比较 genomics 的分析定义一般特征。和顺序打字的进一步的 genomic 采矿允许我们阐明 MCR-1-carrying E。coli E15017 属于顺序类型 ST648 和 coproduces 扩大光谱的 -lactamase (ESBL ) 。给 ST648 与任何一个新德里 metallo 为伙伴所知的事实 -- lactamase 1 或 ESBL,我们的结果与 multidrug 电阻作为流行克隆加亮 ST648 的可能性。 | Huimin Zhang Christopher H. Seward Zuowei Wu Huiyan Ye Youjun Feng | 2016 | Science Bulletin2016，61，11： | 4 |
| 7 | Grain Growth Behavior of 5Cr21Mn9Ni4N Steel Micro-Alloyed by Rare Earth显示文摘Grain growth behavior of 5Cr21Mn9Ni4NRE steel was experimentally studied at various solid solution treatment temperatures and holding for different times. The experimental results show that the 5Cr21Mn9Ni4NRE steel has the feature of sharp austenite grain coarsening after solid solution treatment at the temperature above 1150 ℃. RE added in the steel has the benefit to restrain grain growth and increase grain growth activation energy. Grain growth mechanism is self-diffusion controlling process. The dispersed carbo-nitride in the steel greatly restrains grain growth. Solid solution treatment at the temperature below 1100 ℃ leads to little increase of grain size and little decrease of Rockwell hardness for mass carbo-nitride undissolved, whereas solid solution treatment at the temperature above 1150 ℃ leads to rapid grain coarsening and Rockwell hardness decrease. Grain growth exponent increases with the increasing solid solution treatment temperature. | Yu Shichang Wu Shenqing Gong Youjun Gong Yuansheng Lian Mingsheng Ye Gang Cheng Yijun | 2006 | Journal of Rare Earths2006，24，1： | 3 |
| 8 | NONLINEAR MOTION MECHANICS MODEL OF CURVED BLOOD VESSEL显示文摘A geometric model of curved blood vessels is established based on some reason-able hypotheses;the nonlinear motion mechanics model of the curved blood vessel is establishedaccording to basic mechanics laws.This model includes much more physiological factors.It cou-ples the interaction of blood flow with mechanical factors such as the displacement,deformation,strain and stress etc.of the curved blood vessel.It is of great importance for investigating thecirculation rules of the cardiovascular system and the nonlinear pulse wave propagation in curvedblood vessels. | Qiao Aike Liu Youjun Wu Shigui Xu Xiaohu Zeng Yanjun | 2003 | Acta Mechanica Solida Sinica2003，16，1： | 3 |
| 9 | Cosmological parameter fittings with the BICEP2 data显示文摘Combining the latest Planck,Wilkinson Microwave Anisotropy Probe(WMAP),and baryon acoustic oscillation(BAO)data,we exploit the recent cosmic microwave background(CMB)B-mode power spectra data released by the BICEP2 collaboration to constrain the cosmological parameters of the ACDM model,especially the primordial power spectra parameters of the scalar and the tensor modes,ns,s,r,nt.We obtain constraints on the parameters for a lensed ACDM model using the Markov Chain Monte Carlo(MCMC)technique,the marginalized 68%bounds are r=0.03070.09140.1043,ns=0.00610.00610.9617,s=0.01050.00970.0175,nt=0.45150.45790.5198.We find that a blue tilt for nt is favored slightly,but it is still well consistent with flat or even red tilt.Our r value is slightly smaller than the one obtained by the BICEP group,in that we permit nt as a free parameter without imposing the single-field slow roll inflation consistency relation.When we impose this relation,then r=0.04460.06090.2130.For most other parameters,the best fit values and measurement errors are not altered significantly by the introduction of the BICEP2 data. | WU FengQuan LI YiChao LU YouJun CHEN XueLei | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 2 |
| 10 | Plant cell cultures as heterologous bio-factories for secondary metabolite production显示文摘Synthetic biology has been developing rapidly in the last decade and is attracting increasing attention from many plant biologists.The production of high-value plant-specific secondary metabolites is,however,limited mostly to microbes.This is potentially problematic because of incorrect post-translational modification of proteins and differences in protein micro-compartmentalization,substrate availability,chaperone availability,product toxicity,and cytochrome p450 reductase enzymes.Unlike other heterologous systems,plant cells may be a promising alternative for the production of high-value metabolites.Several commercial plant suspension cell cultures from different plant species have been used successfully to produce valuable metabolites in a safe,low cost,and environmentally friendly manner.However,few metabolites are currently being biosynthesized using plant platforms,with the exception of the natural pigment anthocyanin.Both Arabidopsis thaliana and Nicotiana tabacum cell cultures can be developed by multiple gene transformations and CRISPR-Cas9 genome editing.Given that the introduction of heterologous biosynthetic pathways into Arabidopsis and N.tabacum is not widely used,the biosynthesis of foreign metabolites is currently limited;however,therein lies great potential.Here,we discuss the exemplary use of plant cell cultures and prospects for using A.thaliana and N.tabacum cell cultures to produce valuable plant-specific metabolites. | Tong Wu Sandra M.Kerbler Alisdair R.Fernie Youjun Zhang | 2021 | Plant Communications2021，2，5： | 2 |
| 11 | Frequencies and mechanisms of pesticide resistance in Tetranychus urticae field populations in China显示文摘The two-spotted spider mite Tetranychus urticate is an important agricultural pest worldwide.It is extremely polyphagous and has developed resistance to many pesticides.Here,we assessed the pesticide resistance of seven field populations of T.urticae in China,their target site mutations and the activities of their detoxification enzymes.The results showed that abamectin and the traditional pesticides pyridaben,profenofos and bifenthrin had higher resistance or lower toxicity than more recently developed pesticides including chlorfenapyr,spinetoram,cyflumetofen,cyenopyrafen,bifenazate and B-azolemiteacrylic.The frequency of point mutations related to abamectin resistance,G314D in the glutamate-gated chloride channel 1(GluCl1)and G326E in GluC13,ranged 47%-70%and 0%-97%,respectively.The frequency of point mutations in A1215D and F1538I of the voltage-gated sodium channel gene(VGSC),which may increase resistance to pyrethroids,ranged 88%-100%and 10%-100%,respectively.For target sites related to organophosphate resistance,mutation frequencies ranged 25%-92%for G119S and 0%-23%for A201S in the acetycholinesterase gene(Ace).Mutation G126S in the bifenazate resistance-related cytochrome b gene(Cytb)was observed in three of the seven T.urticae populations.Higher activities of detoxification enzymes(P450,GST,CarEs and UGTs)were observed in two T.urticae populations,with significant difference in the XY-SX population.These results provide useful information on the status of pesticide resistance of T.urticae in China and suggest that T.urticae field populations may have multiple resistance mechanisms. | Yan Zhang Dandan Xu Youjun Zhang Qingjun Wu Wen Xie Zhaojiang Guo Shaoli Wang | 2022 | Insect Science2022，29，3： | 1 |
| 12 | Development of a hamster kidney cell line expressing stably T7 RNA polymerase using retroviral gene transfer technology for efficient rescue of infectious foot-and-mouth disease virus显示文摘 | Haixue Zheng Hong Tian Ye Jin Jinyan Wu Youjun Shang Shuanghui Yin Xiangtao Liu QingGe Xie | 2008 | Journal of Virological Methods2008，，1： | 1 |
| 13 | Rapid Detection Co-infections of Classical Swine Fever Virus and Porcine Reproductive and Respiratory Syndrome Virus by One-step Multiplex RT-PCR显示文摘Classical swine fever virus(CSFV) and porcine reproductive and respiratory syndrome virus(PRRSV) have caused immense economic loss in the pig industry and are considered to be the two most important infectious diseases of pigs in the world. A multiplex reverse transcription polymerase chain reaction(multiplex RT-PCR) was developed for CSFV and PRRSV co-infections or infections,respectively. A set of two pairs of primer was designed based on the sequence of nonstructural protein NS54B of CSFV and ORF7 gene of PRRSV. The diagnostic accuracy of multiplex RT-PCR assay was evaluated by using 56 field clinical samples by multiplex RT-PCR,single RT-PCR and sequence analysis;and the specificity of multiplex PCR was verified by using constructed plasmids containing the specific viral target fragments of PRRSV and CSFV,respectively. The results indicated that this assay could reliably differentiate PRRSV and CSFV in co-infection samples. The multiplex RT-PCR developed in this study might provide a new avenue to the rapid the detection of CSFV and PRRSV in one reaction. | TIAN Hong WU Jinyan YAN Chen SHANG Youjun YIN Shuanghui LIU Xiangtao | 2011 | Journal of Northeast Agricultural University(English Edition)2011，18，4： | 1 |
| 14 | Multilayered flexible nanocomposite for hybrid nano- generator enabled by conjunction of piezoelectricity and triboelectricity显示文摘我们制作灵活混合 nanogenerator (HNG ) ,基于 multilayered nanocomposite 材料,它与仅仅二个电极把压电的 nanogenerator (PENG ) 和摩擦电 nanogenerator (TENG ) 集成到单个结构。HNG 启用 nanogenerators 的电的产量的改进。 280 V 的开电路的电压和 25 A 的电线走火电流被 2.5 厘米的 HNG 完成?????匠?洠捩潲楰汬牡??洠捩潲档湡敮????????????????洠捩潲渭湡???????????吗 洠捩潲汦極楤 ?? | Huayang Li Li Su Shuangyang Kuang Youjun Fan Ying Wu Zhong Lin Wang Guang Zhu | 2017 | Nano Research2017，10，3： | 1 |
| 15 | The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus显示文摘 | Hong Tian JingYan Wu YouJun Shang | 2010 | Virology Journal2010，，7： | 1 |
| 16 | High-Temperature Oxidation Behavior of 5Cr21Mn9Ni4N Steel Micro-Alloyed by Rare Earth显示文摘The oxidation resistance of 5Cr21Mn9Ni4N steel micro-alloying by RE at 700~900 ℃ was investigated. The results indicate that oxidation exponent n and oxidation activation energy are increased, and oxidation velocity constant k_p is decreased when 0.2% RE is added in 5Cr21Mn9Ni4N steel. The addition of RE elements does not alter phase constitution of oxidation scale, however it improves the configuration of oxidation scale, and increases thermal stability and adhesivity of oxidation scale, which results in the raise of oxidation resistance of 5Cr21Mn9Ni4N steel at high temperature. The oxidation scale constitutes of refractory steel transfer from manganic oxide mostly to ferric oxide mostly with the increase of temperature, which leads to descend of compactness and desquamation resistance of oxidation scale. The mass increase of ferric oxide in the oxidation scale and the looseness of oxidation scale are the main reason to descend the oxidation resistance of refractory steel. | Yu Shichang Wu Shenqing Gong Youjun Gong Yuansheng Lian Mingsheng Ye Gang Cheng Yijun | 2006 | Journal of Rare Earths2006，24，3： | 1 |
| 17 | A novel cationic collector for silicon removal from collophane using reverse flotation under acidic conditions显示文摘We analyzed a novel cationic collector using chemical plant byproducts,such as cetyltrimethylammonium bromide(CTAB)and dibutyl phthalate(DBP).Our aim is to establish a highly effective and economical process for the removal of quartz from collophane.A microflotation test with a 25 mg·L^(−1)collector at pH value of 6-10 demonstrates a considerable difference in the floatability of pure quartz and fluorapatite.Flotation tests for a collophane sample subjected to the first reverse flotation for magnesium removal demonstrates that a rough flotation process(using a 0.4 kg·t−1 new collector at pH=6)results in a collophane concentrate with 29.33wt%P_(2)O_(5)grade and 12.66wt%SiO2 at a 79.69wt%P_(2)O_(5)recovery,providing desirable results.Mechanism studies using Fourier transform infrared spectroscopy,zeta potential,and contact angle measurements show that the adsorption capacity of the new collector for quartz is higher than that for fluorapatite.The synergistic effect of DBP increases the difference in hydrophobicity between quartz and fluorapatite.The maximum defoaming rate of the novel cationic collector reaches 142.8 mL·min−1.This is considerably higher than that of a conventional cationic collector. | Zhongxian Wu Dongping Tao Youjun Tao Man Jiang Patrick Zhang | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，6： | 1 |
| 18 | Streptococcus suis infection显示文摘 | Youjun Feng Huimin Zhang Zuowei Wu Shihua Wang Min Cao Dan Hu Changjun Wang | 2014 | Virulence2014，，： | 1 |
| 19 | Studies on blended cement with a large amount of fly ash显示文摘 | Xinghua Fu Zhi Wang Wenhong Tao Chunxia Yang Wenping Hou Youjun Dong Xuequan Wu | 2002 | Cement and Concrete Research2002，，7： | 1 |
| 20 | Spectral and biodistributional engineering of deep near-infrared chromophore显示文摘Fluorescence-guided surgery calls for development of near-infrared fluorophores.Despite the wide-spread application and a safe clinical record of Indocyanine Green(ICG),its maximal absorption wavelength at780 nm is rather short and longer-wavelength dyes are desired to exploit such benefits as low phototoxicity and deep penetration depth.Here,we report ECY,a stable deep near-infrared(NIR)fluorochromic scaffold absorbing/emitting at 836/871 nm with a fluorescence quantum yield of 16%in CH_(2)Cl_(2).ECY was further rationally engineered for biological distribution specificity.Analogous bearing different numbers of sulfonate group or a polyethylene glycol chain were synthesized.By screening this focused library upon intravenous injection to BALB/c mice,ECYS2 was identified to be a suitable candidate for bioimaging of organs involved in hepatobiliary excretion,and ECYPEG was found to be a superior candidate for vasculature imaging.They have potentials in intraoperative imaging. | Yan Dong Xicun Lu Yi Li Weichao Chen Lei Yin Jie Zhao Xinru Hu Xinran Li Zuhai Lei Yuyang Wu Hao Chen Xiao Luo Xuhong Qian Youjun Yang | 2023 | Chinese Chemical Letters2023，34，9： | 0 |
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