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| 1 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 2 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 3 | Diffusion tensor imaging assesses white matter injury in neonates with hypoxic-ischemic encephalopathy显示文摘With improvements in care of at-risk neonates, more and more children survive. This makes it increasingly important to assess, soon after birth, the prognosis of children with hypoxic-ischemic encephalopathy. Computed tomography, ultrasound, and conventional magnetic resonance imaging are helpful to diagnose brain injury, but cannot quantify white matter damage. In this study, ten full-term infants without brain injury and twenty-two full-term neonates with hypoxic-ischemic encephalopathy(14 moderate cases and 8 severe cases) underwent diffusion tensor imaging to assess its feasibility in evaluating white matter damage in this condition. Results demonstrated that fractional anisotropy, voxel volume, and number of fiber bundles were different in some brain areas between infants with brain injury and those without brain injury. The correlation between fractional anisotropy values and neonatal behavioral neurological assessment scores was closest in the posterior limbs of the internal capsule. We conclude that diffusion tensor imaging can quantify white matter injury in neonates with hypoxic-ischemic encephalopathy. | Hong-xin Li Xing Feng Qian Wang Xuan Dong Min Yu Wen-juan Tu | 2017 | Neural Regeneration Research2017，12，4： | 19 |
| 4 | Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol显示文摘Menopause is associated with dyslipidemia and an increased risk of cardio-cerebrovascular disease.The classic view assumes that the underlying mechanism of dyslipidemia is attributed to an insufficiency of estrogen.In addition to a decrease in estrogen, circulating follicle-stimulating hormone (FSH)levels become elevated at menopause.In this study,we find that blocking FSH reduces serum cholesterol via inhibiting hepatic cholesterol biosynthesis.First,epidemiological results show that the serum FSH levels are positively correlated with the serum total cholesterol levels,even after adjustment by considering the effects of serum estrogen,in addition,the prevalence of hypercholesterolemia is significantly higher in peri-menopausal women than that in premenopausal women.Furthermore,we generated a mouse model of FSH elevation by intraperitoneally injecting exogenous FSH into ovariectomized (OVX)mice,in which a normal level of estrogen (E2)was maintained by exogenous supplementation. Consistently,the results indicate that FSH,independent of estrogen,increases the serum cholesterol level in this mouse model. Moreover,blocking FSH signaling by anti-FSHβ antibody or ablating the FSH receptor (FSHR)gene could effectively prevent hypercholesterolemia induced by FSH injection or high-cholesterol diet feeding.Mechanistically,FSH,via binding to hepatic FSHRs, activates the Gi2α/β-arrestin-2/Akt pathway and subsequently inhibits the binding of FoxO1 with the SREBP-2 promoter,thus preventing FoxO1 from repressing SREBP-2 gene transcription.This effect,in turn,results in the upregulation of SREBP-2,which drives HMGCR nascent transcription and de novo cholesterol biosynthesis,leading to the increase of cholesterol accumulation.This study uncovers that blocking FSH signaling might be a new strategy for treating hypercholesterolemia during menopause, particularly for women in peri-menopause characterized by FSH elevation only. | Yanjing Guo Meng Zhao Tao Bo Shizhan Ma Zhongshang Yuan Wenbin Chen Zhao He Xu Hou Jun Liu Zhenhai Zhang Qiang Zhu Qiangxiu Wang Xiaoyan Lin Zhongli Yang Min Cui Lu Liu Yujie Li Chunxiao Yu Xiaoyi Qi Qian Wang Haiqing Zhang Qingbo Guan Lifang Zhao Shimeng Xuan Huili Yan Yanliang Lin Li Wang Qihang Li Yongfeng Song Ling Gao Jiajun Zhao | 2019 | Cell Research2019，29，2： | 16 |
| 5 | Experiment on Leader Propagation Characteristics of Air Gaps in UHVDC Transmission Towers Under Positive Switching Impulse Voltages显示文摘Rapid developments in EHV/UHV transmission systems require a deeper understanding of the mechanism of long air gap discharge.Leader propagation is one of the main processes in long gap breakdown.In this paper,the leader propagation characteristics of real size±800 kV UHVDC transmission tower gaps under positive switching impulse voltages(185/2290μs)are investigated.An integrated observation platform consisting of an impulse voltage divider,a coaxial shunt,a high-speed video camera,and a set of integrated optical electric field sensors(IOES),is established.The waveforms of impulse voltage,discharge current,electric field variation at specific positions,and time-resolved photographs of discharge morphology are recorded.Axial leader velocity and the relationship between leader advancements and injected charge are obtained.The typical value of leader stable propagation velocity is 1.7–2.2 cm/μs,which varies slightly with the gap length and applied voltage amplitude.The leader velocity in the re-illumination process is much higher,and is seen as varying from 5 cm/μs to 30 cm/μs,with an average value around 10 cm/μs.The charge in leader channel per unit length is 20–40μC/m,which illustrates a near-direct proportion relationship between discharge current and leader velocity.The observed parameters are important for further simulation of the tower gap breakdown processes. | Xuan Zhou She Chen Hai Wang Rong Zeng Chijie Zhuang Junjie Yu Yujian Ding | 2015 | CSEE Journal of Power and Energy Systems2015，1，3： | 12 |
| 6 | Effects of bamboo vinegar powder on growth performance and mRNA expression levels of interleukin-10,interleukin-22,and interleukin-25 in immune organs of weaned piglets显示文摘The aim of this study was to explore the effects of bamboo vinegar powder on growth performance,diarrhea situation and mRNA expression levels of cytokines i.e., interleukin-10(IL-10), interleukin-22(IL-22), and interleukin-25(IL-25) in immune organs of weaned piglets, and to accumulate theoretical data for the application of bamboo vinegar powder in weaned piglet production. Forty-five crossbred(Duroc × Landrace × Yorkshire, all male) weaned piglets with similar body weight(6.74 ± 0.17 kg) at 31 days of age were randomly assigned to 5 treatments with 3 replicates per treatment and 3 piglets in each replicate. The five treatments were as follows: CON(a basal diet), ANT(the basal diet + 0.12% antibiotics),BV1(the basal diet + 0.1% bamboo vinegar powder), BV5(the basal diet + 0.5% bamboo vinegar powder),BV10(the basal diet + 1.0% bamboo vinegar powder). This experiment lasted 35 days. The growth performance and diarrhea situation were recorded. The relative mRNA expression levels of IL-10, IL-22 and IL-25 in liver, spleen, duodenum and mesenteric lymph nodes were detected by real-time PCR. Feed:gain of BV5 was significantly lower than that of CON(P < 0.05). In comparison with CON, diarrhea rate and diarrhea index of BV1 and BV5 all tended to decrease(P < 0.1). Compared with CON, mRNA expression level of IL-10 in liver of ANT tended to be lower(P < 0.1) and these of BV1, BV5 and BV10 were significantly reduced(P < 0.05). The mRNA expression levels of IL-10 in duodenum of ANT, BV1, BV5 and BV10 were all lower than those of CON, of which BV10 had significantly decreased IL-10 mRNA expression in duodenum(P < 0.05). The mRNA expression levels of IL-22 in duodenum of ANT, BV1, BV5 and BV10 all tended to be inhibited compared with CON(P < 0.1). With the increase of bamboo vinegar powder dosage, mRNA expression levels of IL-25 in spleen and mesenteric lymph nodes of BV1, BV5 and BV10 tended to be up-regulated. Overall, bamboo vinegar powder could improve growth performance,and regulate mRNA expression levels of IL-10, IL-22 and IL-25 in immune organs of weaned piglets. The dosage at 0.5% showed optimum effects. | Yongjiu Huo Zhengxu Liu Han Xuan Chunbin Lu Lihuai Yu Wenbin Bao Guoqi Zhao | 2016 | Animal Nutrition2016，，2： | 11 |
| 7 | Effects of Angelica dahurica on obesity and fatty liver in mice显示文摘Angelica dahurica(A. dahurica) is a traditional Chinese medicinal plant being used in clinical practice. The present study demonstrated that A. dahurica could reduce white-fat weight in high-fat-diet hyperlipidemic mice, decrease total cholesterol and triglyceride concentrations in the livers of both high-fat-diet and Triton WR1339 induced hyperlipidemic mice, and enhance the total hepatic lipase activities of them. These findings were further supported by the results derived from the experiments with Hep G2 cells in vitro. In addition, the proteins related to lipids metabolism were investigated using LC-MS/MS, indicating that genes of lipid metabolism and lipid transport were regulated by A. dhurica. The results from LC-MS/MS were further conformed by Western blot and real time PCR assays. A. dahurica could down-regulate the expression of catalase(CAT) and sterol carrier protein2(SCP2) and up-regulate the expression of lipid metabolism related genes-lipase member C(LIPC) and peroxisome proliferator-activated receptor gamma(PPARγ). In the Triton WR1339 mouse liver and Hep G2 cells in vitro, A. dahurica was able to increase the expression of LIPC and PPARγ, confirming the results from in vivo experiments. Imperatorin showed the same activity as A. dahurica, suggesting it was one of the major active ingredients of the herb. In conclusion, our work represented a first investigation demonstrating that A. dahurica was able to regulate lipid metabolism and could be developed as a novel approach to fighting against fatty liver and obesity. | LU Xi YUAN Zhi-Yi YAN Xiao-Jin LEI Fan JIANG Jing-Fei YU Xuan YANG Xiu-Wei XING Dong-Ming DU Li-Jun | 2016 | Chinese Journal of Natural Medicines2016，14，9： | 11 |
| 8 | Characteristics of ozone pollution and the sensitivity to precursors during early summer in central plain, China显示文摘In this study,we conducted an observation experiment from May 1 to June 30,2018 in Zhengzhou,a major city in central China,where ground ozone(O3)pollution has become serious in recent years.The concentrations of O3 and its precursors,as well as H2O_(2) and meteorological data were obtained from the urban site(Yanchang,YC),suburban(Zhengzhou University,ZZU)and background sites(Ganglishuiku,GLSK).Result showed that the rates of O3 concentration exceeded Chinese National Air Quality Standard GradeⅡ(93.3 ppbv)were 59.0%,52.5%,and 55.7%at the above three sites with good consistency,respectively,indicating that O3 pollution is a regional problem in Zhengzhou.The daily peak O3 appeared at 15:00-16:00,which was opposite to VOCs,NOx,and CO and consistent with H2O_(2).The exhaustive statistical analysis of meteorological factors and chemical effects on O3 formation at YC was advanced.The high concentration of precursors,high temperature,low relative humidity,and moderately high wind speed together with the wind direction dominated by south and southeast wind contribute to urban O3 episodes in Zhengzhou.O3 formation analysis showed that reactive alkenes such as isoprene and cis-2-butene contributed most to O3 formation.The VOCs/NOx ratio and smog production model were used to determine O3-VOC-NOx sensitivity.The O3 formation in Zhengzhou during early summer was mainly under VOC-limited and transition regions alternately,which implies that the simultaneous emission reduction of alkenes and NOx is effective in reducing O3 pollution in Zhengzhou. | Yasong Li Shasha Yin Shijie YU Ling Bai Xudong Wang Xuan Lu Shuangliang Ma | 2021 | Journal of Environmental Sciences2021，33，1： | 10 |
| 9 | High degree of pharmacokinetic compatibility exists between the five-herb medicine XueBiJing and antibiotics comedicated in sepsis care显示文摘Managing the dysregulated host response to infection remains a major challenge in sepsis care. Chinese treatment guideline recommends adding Xue Bi Jing, a five-herb medicine, to antibioticbased sepsis care. Although adding Xue Bi Jing further reduced 28-day mortality via modulating the host response, pharmacokinetic herbedrug interaction is a widely recognized issue that needs to be studied.Building on our earlier systematic chemical and human pharmacokinetic investigations of Xue Bi Jing, we evaluated the degree of pharmacokinetic compatibility for Xue Bi Jing/antibiotic combination based on mechanistic evidence of interaction risk. Considering both Xue Bi Jing-antibiotic and antibiotic-Xue Bi Jing interaction potential, we integrated informatics-based approach with experimental approach and developed a compound pair-based method for data processing. To reflect clinical reality, we selected for study Xue Bi Jing compounds bioavailable for drug interactions and 45 antibiotics commonly used in sepsis care in China. Based on the data of interacting with drug metabolizing enzymes and transporters, no Xue Bi Jing compound could pair, as perpetrator, with the antibiotics. Although some antibiotics could,due to their inhibition of uridine 50-diphosphoglucuronosyltransferase 2 B15, organic anion transporters1/2 and/or organic anion-transporting polypeptide 1 B3, pair with senkyunolide I, tanshinol and salvianolic acid B, the potential interactions(resulting in increased exposure) are likely desirable due to these Xue Bi Jing compounds’ low baseline exposure levels. Inhibition of aldehyde dehydrogenase by 7 antibiotics probably results in undesirable reduction of exposure to protocatechuic acid from Xue Bi Jing.Collectively, Xue Bi Jing/antibiotic combination exhibited a high degree of pharmacokinetic compatibility at clinically relevant doses. The methodology developed can be applied to investigate other drug combinations. | Jian Li Olajide E.Olaleye Xuan Yu Weiwei Jia Junling Yang Chuang Lu Songqiao Liu Jingjing Yu Xiaona Duan Yaya Wanga Kai Dong Rongrong He Chen Cheng Chuan Li | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 10 |
| 10 | Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence显示文摘Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of 'Darmor-bzh'and 'Tapidor,'respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding. | Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang | 2019 | Molecular Plant2019，12，1： | 10 |
| 11 | Detection of Pseudorabies Virus Antibodies in Human Encephalitis Cases显示文摘Pseudorabies virus(PRV),a veterinary pathogen that infects domestic animals as well as wild animals such as wild boar and feral swine,was recently reported to infect human and led to endophthalmitis and encephalitis.A retrospective seroepidemiologic survey was conducted using 1,335 serum samples collected from patients with encephalitis and ELISA positive rates were 12.16%,14.25%,and 6.52%in 2012,2013,and 2017,respectively. | LI Xiang Dong FU Shi Hong CHEN Ling Yan LI Fan DENG Jun Hua LU Xuan Cheng WANG Huan Yu TIAN Ke Gong | 2020 | Biomedical and Environmental Sciences2020，33，6： | 9 |
| 12 | Generation of graphene-based aerogel microspheres for broadband and tunable high-performance microwave absorption by electrospinning-freeze drying process显示文摘 | Fanbin Meng Huagao Wang Wei Zijian Chen Tian Li Chunyuan Li Yu Xuan Zuowan Zhou | 2018 | Nano Research2018，11，5： | 9 |
| 13 | Coupling effects of coal pillars of thick coal seams in large-space stopes and hard stratum on mine pressure显示文摘Concerning the issue of mine pressure behaviors occurred in fully mechanized caving mining of thick coal seams beneath hard stratum in Datong Mining Area, combined with thin and thick plate theory, the paper utilizes theoretical analysis, similar experiments, numerical simulations and field tests to study the influence of remaining coal pillars in Jurassic system goaf on hard stratum fractures, as well as mine pressure behaviors under their coupling effects. The paper concludes the solution formula of initial fault displacement in hard stratum caused by remaining coal pillars. Experiments prove that coupling effects can enhance mine pressure behaviors on working faces. When inter-layer inferior key strata fractures, mine pressure phenomenon such as significant roof weighting steps and increasing resistance in support.When inter-layer superior key strata fractures, the scope of overlying strata extends to Jurassic system goaf, dual-system stopes cut through, and remaining coal pillars lose stability. As a result, the bottom inferior key strata also lose stability. It causes huge impacts on working face, and the second mine pressure behaviors. These phenomena provide evidence for research on other similar mine strata pressure behaviors occurred in dual-system mines with remaining coal pillars. | Xia Binwei Jia Jinlong Yu Bin Zhang Xuan Li Xiaolong | 2017 | International Journal of Mining Science and Technology2017，27，6： | 9 |
| 14 | Hard X-ray Imager (HXI) onboard the ASO-S mission显示文摘Hard X-ray Imager(HXI)is one of the three scientific instruments onboard the Advanced Spacebased Solar Observatory(ASO-S)mission,which is proposed for the 25th solar maximum by the Chinese solar community.HXI is designed to investigate the non-thermal high-energy electrons accelerated in solar flares by providing images of solar flaring regions in the energy range from 30 keV to 200 keV.The imaging principle of HXI is based on spatially modulated Fourier synthesis and utilizes about 91 sets of bi-grid sub-collimators and corresponding LaBr3 detectors to obtain Fourier components with a spatial resolution of about 3 arcsec and a time resolution better than 0.5 s.An engineering prototype has been developed and tested to verify the feasibility of design.In this paper,we present background,instrument design and the development and test status of the prototype. | Zhe Zhang Deng-Yi Chen Jian Wu Jin Chang Yi-Ming Hu Yang Su Yan Zhang Jian-Ping Wang Yao-Ming Liang Tao Ma Jian-Hua Guo Ming-Sheng Cai Yong-Qiang Zhang Yong-Yi Huang Xiao-Yan Peng Zong-Bin Tang Xuan Zhao Hong-He Zhou Lian-Guo Wang Jing-Xing Song Miao Ma Guang-Zhou Xu Jian-Feng Yang Di Lu Ying-Hong He Jin-You Tao Xiao-Long Ma Bao-Gang Lv Yan-Ping Bai Cai-Xia Cao Yu Huang Wei-Qun Gan | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 9 |
| 15 | Dietary high-fat lard intake induces thyroid dysfunction and abnormal morphology in rats显示文摘 | Shan-shan SHAO Yuan-fei ZHAO Yong-feng SONG Chao XU Jian-mei YANG Shi-meng XUAN Hui-li YAN Chun-xiao YU Meng ZHAO Jin XU Jia-jun ZHAO | 2014 | Acta Pharmacologica Sinica2014，35，11： | 9 |
| 16 | Diabetic foot ulcers:Classification,risk factors and management显示文摘Diabetic foot ulceration is a devastating complication of diabetes that is associated with infection,amputation,and death,and is affecting increasing numbers of patients with diabetes mellitus.The pathogenesis of foot ulcers is complex,and different factors play major roles in different stages.The refractory nature of foot ulcer is reflected in that even after healing there is still a high recurrence rate and amputation rate,which means that management and nursing plans need to be considered carefully.The importance of establishment of measures for prevention and management of DFU has been emphasized.Therefore,a validated and appropriate DFU classification matching the progression is necessary for clinical diagnosis and management.In the first part of this review,we list several commonly used classification systems and describe their application conditions,scope,strengths,and limitations;in the second part,we briefly introduce the common risk factors for DFU,such as neuropathy,peripheral artery disease,foot deformities,diabetes complications,and obesity.Focusing on the relationship between the risk factors and DFU progression may facilitate prevention and timely management;in the last part,we emphasize the importance of preventive education,characterize several of the most frequently used management approaches,including glycemic control,exercise,offloading,and infection control,and call for taking into account and weighing the quality of life during the formulation of treatment plans.Multidisciplinary intervention and management of diabetic foot ulcers(DFUs)based on the effective and systematic combination of these three components will contribute to the prevention and treatment of DFUs,and improve their prognosis. | Xuan Wang Chong-Xi Yuan Bin Xu Zhi Yu | 2022 | World Journal of Diabetes2022，13，12： | 9 |
| 17 | Involvement of the Mitochondrion-dependent and the Endoplasmic Reticulum Stress-signaling Pathways in Isoliquiritigenin-induced Apoptosis of HeLa Cell显示文摘Objective Isoliquiritigenin(ISL),a licorice chalconoid,is considered to be a bioactive agent with chemopreventive potential.This study investigates the mechanisms involved in ISL-induced apoptosis in human cervical carcinoma HeLa cells.Methods Cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT)assay.Apoptosis was determined by flow cytometry using an Annexin V-FITC Apoptosis Detection Kit.The intracellular ROS levels were assessed using a 2,7-dichlorofluorescein probe assay.The mitochondrial membrane potential was measured with the dual-emission potential-sensitive probe 5,5',6,6'-tetra-chloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide(JC-1).The degradation of poly-ADP-ribose polymerase(PARP)protein,the phosphorylation of PKR-like ER kinase(PERK),the phosphorylation of the α-subunit of eukaryotic initiation factor 2(eIF2α),the expression of the 78 kD glucose-regulated protein(GRP 78),and the activation of caspase-12 were analyzed via western blot analysis.Results ISL significantly inhibited the proliferation,the increase in ROS levels and apoptotic rates of HeLa cells in a concentration-dependent manner.Moreover,ISL induced mitochondrial dysfunction,caspase activation,and PARP cleavage,which displayed features of mitochondria dependent on apoptotic signals.Besides,exposure of HeLa cells to ISL triggered endoplasmic reticulum(ER)stress,as indicated by the increase in p-eIF2α and GRP78 expression,ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12.Conclusion The findings from our study suggest that ISL-induced oxidative stress causes HeLa cell apoptosis via the mitochondrion-dependent and the ER stress-triggered signaling pathways. | YUAN Xuan ZHANG Bo GAN Lu WANG Zhen Hua YU Ba Cui LIU Liang Liang ZHENG Qiu Sheng WANGZhi Ping | 2013 | Biomedical and Environmental Sciences2013，26，4： | 8 |
| 18 | Feasibility and advantage of biofilm-electrode reactor for phenol degradation显示文摘The new biofilm-electrode method was used for the phenol degradation,because of its low current requirement.The biofilm-electrode reactor consisted of immobilized degrading bacteria on Ti electrode as cathode and Ti/PbO2 electrode as anode.With the biofilmelectrode reactor in a divided electrolytic cell,the phenol degradation rate could achieve 100%at 18 h which was higher than using traditional methods,such as biological or electrochemical methods.Chemical oxygen demand(COD)removal rate of the biofilmelectrode reactor was also greater than that using biological and electrochemical method,and could reach 80%at 16 h.The results suggested that the biofilm-electrode reactor system can be used to treat wastewater with phenol. | ZHANG Xuena HUANG Weimin WANG Xuan GAO Yu LIN Haibo | 2009 | Journal of Environmental Sciences2009，21，9： | 8 |
| 19 | Mechanism underlying berberine's effects on HSP70/TNFα under heat stress:Correlation with the TATA boxes显示文摘Heat stress can stimulate an increase in body temperature,which is correlated with increased expression of heat shock protein 70(HSP70) and tumor necrosis factor α(TNFα).The exact mechanism underlying the HSP70 and TNFα induction is unclear.Berberine(BBR) can significantly inhibit the temperature rise caused by heat stress,but the mechanism responsible for the BBR effect on HSP70 and TNFα signaling has not been investigated.The aim of the present study was to explore the relationship between the expression of HSP70 and TNFα and the effects of BBR under heat conditions,using in vivo and in vitro models.The expression levels of HSP70 and TNFα were determined using RT-PCR and Western blotting analyses.The results showed that the levels of HSP70 and TNFα were up-regulated under heat conditions(40 °C).HSP70 acted as a chaperone to maintain TNFα homeostasis with rising the temperature,but knockdown of HSP70 could not down-regulate the level of TNFα.Furthermore,TNFα could not influence the expression of HSP70 under normal and heat conditions.BBR targeted both HSP70 and TNFα by suppressing their gene transcription,thereby decreasing body temperature under heat conditions.In conclusion,BBR has a potential to be developed as a therapeutic strategy for suppressing the thermal effects in hot environments. | JIANG Jing-Fei LEI Fan YUAN Zhi-Yi WANG Yu-Gang WANG Xin-Pei YAN Xiao-Jin YU Xuan XING Dong-Ming DU Li-Jun | 2017 | Chinese Journal of Natural Medicines2017，15，3： | 8 |
| 20 | Incidence of C-shaped root canal systems in mandibular second molars in the native Chinese population by analysis of clinical methods显示文摘The aims of the study were to investigate the incidence of C-shaped root canal systems in mandibular second molars in a native Chinese population using radiography and clinical examination under microscope and to compare the relative efficacies of these methods.For the recognition of C-shaped root canal system,1 146 mandibular second molars were selected and examined.Teeth with C-shaped canal systems were categorized by using the radiographic classification criteria and the modified Melton's method.C-shaped canals were identified in 397(34.64%) mandibular second molars by radiography(type I,31.23%;type II,38.29%;type III,30.48%).Clinical examination showed that 449(39.18%) cases exhibited C-shaped canal systems(C1,22.94%;C2,48.11%;C3a,15.59%;C3b,13.36%).As for the result of the radiographic and clinical combined examination,C-shaped root canals were found in 473(41.27%) mandibular second molars(C1,21.78%;C2,45.67%;C3a,16.70%;C3b,15.86%).The incidence of C-shaped root canal diagnosed by radiographic method was statistically different from that by clinical examination and the combined examination(P,0.05).The study indicated a high incidence of C-shaped canal system in a Chinese population.The combination of microscopic and radiographic examination is an effective method in identifying the C-shaped root canal system. | Yan Wang Jing Guo Hai-Bing Yang Xuan Han Ying Yu | 2012 | International Journal of Oral Science2012，4，3： | 8 |
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