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| 1 | A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2显示文摘The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as‘positive’.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the‘gene scissors’clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations. | Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar AAl-Hartomy Swelm Wageh Abdullah GAl-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang | 2022 | National Science Review2022，9，8： | 3 |
| 2 | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant显示文摘Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing techniques.Herein,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 variant.To enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold nanoparticles.Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays.The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system.Furthermore,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing. | Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy SWageh Abdullah GAl-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang | 2022 | Nano-Micro Letters2022，14，10： | 2 |
| 3 | Characteristics of root pullout resistance of Caragana korshinskii Kom.in the loess area of northeastern Qinghai-Tibet Plateau,China显示文摘Roots exert pullout resistance under pullout force,allowing plants to resist uprooting.However,the pullout resistance characteristics of taproot-type shrub species of different ages remain unclear.In this study,in order to improve our knowledge of pullout resistance characteristics of taproot systems of shrub species,we selected the shrub species Caragana korshinskii Kom.in different growth periods as the research plant and conducted in situ root pullout test.The relationships among the maximum pullout resistance,peak root displacement,shrub growth period,and aboveground growth indices(plant height and plant crown breadth)were analyzed,as well as the mechanical process of uprooting.Pullout resistance of 4-15 year-old C.korshinskii ranged from 2.49(±0.25)to 14.71(±4.96)kN,and the peak displacement ranged from 11.77(±8.61)to 26.50(±16.09)cm.The maximum pullout resistance and the peak displacement of roots increased as a power function(R^(2)=0.9038)and a linear function(R^(2)=0.8242)with increasing age,respectively.The maximum pullout resistance and the peak displacement increased with increasing plant height;however,this relationship was not significant.The maximum pullout resistance increased exponentially(R^(2)=0.5522)as the crown breadth increased.There was no significant relationship between the peak displacement and crown breadth.The pullout resistance and displacement curve were divided into three stages:the initial nonlinear growth,linear growth,and nonlinear stages.Two modes of failure of a single root occurred when the roots were subjected to vertical loading forces:the synchronous breakage mode and the periderm preferential breakage mode.These findings provide a foundation for further investigation of the soil reinforcement and slope protection mechanisms of this shrub species in the loess area of northeastern Qinghai-Tibet Plateau,China. | LIU Yabin SHI Chuan YU Dongmei WANG Shu PANG Jinghao ZHU Haili LI Guorong HU Xiasong | 2022 | Journal of Arid Land2022，14，7： | 2 |
| 4 | Connection between earthquakes and deep fluids revealed by magnetotelluric imaging in Songyuan,China显示文摘Songyuan is the most earthquake prone area in northeast China.Since 2006,earthquakes have occurred in the area in the form of swarms,with a maximum magnitude of M_L5.8.There is much controversy about the cause of the Songyuan earthquakes.We attempted to determine the cause using a three-dimensional electrical conductivity structure inverted from a regional network of magnetotelluric data in the Songyuan area.The L-BFGS inversion method was applied,with a fullimpedance tensor data set used as the inversion input.Combined with an evaluation of the earthquake locations,the resistivity model revealed a northeast-oriented hidden fault running through the Songyuan earthquake area(SEA),which was speculated to be the preexisting Fuyu-Zhaodong Fault(FZF).Our resistivity model also found an apparent lithospheric low-resistivity anomaly beneath the earthquake area,which breached the high-resistivity lithospheric mantle and stalled at the base of the crust.A petrophysical analysis showed that this lower crustal low-resistivity anomaly was most likely attributed to hydrated partial melting,which could release water into the lower crust during later magma emplacements.While weakening the strength of the FZF,these ascending fluids also increased the pore pressure in the fault,further reducing the shear strength of the fault.Shear stress action(a fault strike component of the east-west regional compress),together with possible near-surface disturbances,may drive the fault to slip and trigger the earthquakes in Songyuan.It is possible that the continuous replenishment of fluids from the deeper mantle forces the Songyuan earthquakes into the form of swarms.We infer that the Songyuan earthquakes could be attributed to a combination of preexisting faults,regional stress,and deep fluids associated with plate subduction,and near surface disturbances might induce the earthquakes in advance.The Songyuan earthquakes are inherently induced earthquakes,fed by deep fluids. | Yu TANG Aihua WENG Yue YANG Shiwen LI Jianjun NIU Yanhui ZHANG Yabin LI Jianping LI | 2021 | Science China Earth Sciences2021，64，1： | 1 |
| 5 | Ac response of an interacting quantum dot in a nonequilibrium state显示文摘 | Yu Yabin Au Yeung T C Shangguan W Z | 2001 | Phys Rev B2001，63，20： | 1 |
| 6 | Role of wide contacts in the at response of a double-barrier stractare显示文摘 | Quan Jun Yu Yabin Tang Y H | 2006 | Phys Rev B2006，73，19： | 1 |
| 7 | Seroprevalence and risk factors of human cytomegalovirus infection in the eastern Chinese population显示文摘 | Ping Zhao Daoxin Ma Fuzhong Xue Chunyan Ji Shukang Wang Xiaomei Zhang Yabin Zhou Xiuping Yu | 2009 | Archives of Virology2009，，4： | 1 |
| 8 | Seroprevalence and risk factors of human cytomegalovirus infection in the eastern Chinese population显示文摘 | Ping Zhao Daoxin Ma Fuzhong Xue Chunyan Ji Shukang Wang Xiaomei Zhang Yabin Zhou Xiuping Yu | 2009 | Archives of Virology2009，，4： | 1 |
| 9 | Effect of super-chilling storage on maintenance of quality and freshness of the Pacific oyster(Crassostrea gigas)显示文摘The quality changes of shelled Pacific oysters(Crassostrea gigas)were examined in relation to the effects of superchilling storage at-1 C for 28 d by measuring changes in biochemical properties(microbial analysis,adenosine triphosphate(ATP)-related compounds,pH,free amino acids)and sensory evaluations in this study.The results indicated that microorganism growth was significantly inhibited during superchilling storage.Adenosine diphosphate(ADP)and adenosine monophosphate(AMP)accumulated while ATP rapidly decreased in the adductor muscle.ATP and ADP were the primary components in the other 3 tissues including mantle,gill,and body trunk of oysters,and they remained relatively stable over time.The pH and adenylate energy charge in the adductor muscle could be utilized as freshness indicators for shelled oysters.However,there were no significant differences(P>0.05)among the free amino acids during whole storage.According to the sensory evalu-ations,oysters could be alive and tolerated up to 21 d at-1℃storage.The study demonstrated that superchilling storage at-1℃could better maintain the eating quality of shelled oysters and the shelf life was extended to 21 d. | Shiliang Dong Yabin Niu Huamao Wei Yumeng Lin Xin Lu Tetsuro Yamashita Kefeng Yu Koichi Takaki Chunhong Yuan | 2023 | Food Quality and Safety2023，7，2： | 0 |
| 10 | The molecular orientation of PNB A on silver nanoparticles显示文摘The molecular orientations of p-nitrobenzoic acid (PNBA) on the surface of silver nanoparticles and silver mirror have been investigated by using surface enhanced Raman scattering (SERS) and UV-visible absorption spectroscopy. In the mixed system of silver colloid/PNBA/ethanol, the orientation of PNBA on silver nanoparticles remarkably different from that on silver mirror was observed, and it mainly resulted from the cooperation effect of the adsorption to surface active sites on silver under the pressure of aqueous molecules. This result is of some significance for further studying surface-interfacial structures and properties of silver nanoparticles, and the technique used in this note is feasible to get the SERS spectra of some molecules insoluble in aqueous solution. | Lijun Guo Juanjuan Yu Yuncai Li Yuzong Gu Yabin Huang Yujun Mo | 2000 | Chinese Science Bulletin2000，45，16： | 0 |
| 11 | Influence of an equimolar amount of aluminium and phosphorus on spectroscopic properties of neodymium under local phosphorus environment in silica glass显示文摘To exhibit large emission cross sections of Nd in silica glass, Nd surroundings were regulated in AI/P codoped silica glass prepared by our multi-step method. The influence of an equimolar amount of AI and P on spectroscopic properties was studied under the local phosphorus coordination environment of Nd.The uniformity of composition distribution was characterized by energy dispersion spectra(EDS) and the coordination environment of Nd ions was characterized by the electron paramagnetic resonance(EPR)spectra. The results indicate that the composition distribution is of high uniformity and Nd has a stronger interaction with P atoms than AI atoms regardless of Al/P molar ratio. Local phosphorus coordination environment of Nd ions in silica glass is achieved. Large σ_(em) can be achieved when Al or P is excessive,and larger σ_(em) is more likely achieved when P is much more than Al. This work brings new possibilities for obtaining large stimulated cross sections of Nd in silica glass. | Yabin Cao Fan Wang Chongyun Shao Yu Yue Lili Hu Chunlei Yu | 2018 | Journal of Rare Earths2018，36，10： | 0 |
| 12 | Pressure shear assisted extraction of polysaccharide from Auricularia auricula and its biological activities显示文摘In this paper,using to the comprehensive scoring method,pressure shear assisted extraction was used to prepare polysaccharide from Auricularia auricula(PS-AAP)by orthogonal test to optimize the extraction.The highest comprehensive score(90.70)was obtained under 1:70 g/mL solid-liquid ratio,110°C reaction temperature,60 min reaction time and 1.0 MPa reaction pressure.Compared to AAP by water bath(WB-AAP),PS-AAP led to a higher extraction efficiency and decreased the relative molecular weight.In addition,infrared spectrum(FT-IR)analysis showed that pressure shear assisted did not change the polysaccharide structure.Scanning electron microscope(SEM)showed that PS-AAP was smaller and more broken by pressure shear assisted.Furthermore,the ratio of glucose to mannose in PS-AAP was relatively higher than WB-AAP,which also proved that PS-AAP had certain antioxidant and hypoglycemic activities.Compared with WB-AAP,the inhibitionα-glucosidase andα-amylase activity of PS-AAP increased by 11.37%and 24.43%respectively.These results revealed that PS-AAP has the poten-tial value of being a antioxidant and hypoglycemic agent. | Yuetong Chen Wenhao Yu Shanshan Zhang Yabin Niu Yanrong Zhang Tingting Liu | 2023 | Journal of Future Foods2023，3，1： | 0 |
| 13 | Clinical outcomes of primary rotating-hinge knee arthroplasty for knees with severe deformity显示文摘Rotating-hinge total knee prostheses are often used for the treatment of global instability or severe bone loss around the knee,which is often accompanied by severe deformity.1 Competent and functional collateral ligaments are a prerequisite for performance of conventional total knee arthroplasty (TKA).In cases of primary surgery for knees with severe deformities or in revision surgery for knees with substantial bone loss,standard condylar implants do not allow for proper stabilization of the joint.Under these circumstances,condylar TKA designs will fail within a short period of time,or other complications such as anterior knee pain will occur soon after surgery. | Zhang Fujiang Liu Yabin Xiao Yu Liu Wenbin | 2014 | Chinese Medical Journal2014，，9： | 0 |
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