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| 1 | Transcriptome sequencing analysis for the identification of stable lncRNAs associated with bovine Staphylococcus aureus mastitis显示文摘Background:Staphylococcus aureus(S.aureus)mastitis is one of the most difficult diseases to treat in lactating dairy cows worldwide.S.aureus with different lineages leads to different host immune responses.Long non-coding RNAs(lncRNAs)are reported to be widely involved in the progress of inflammation.However,no research has identified stable lncRNAs among different S.aureus strain infections.In addition,folic acid(FA)can effectively reduce inflammation,and whether the inflammatory response caused by S.aureus can be reduced by FA remains to be explored.Methods:lncRNA transcripts were identified from Holstein mammary gland tissues infected with different concentrations of S.aureus(in vivo)and mammary alveolar cells(Mac-T cells,in vitro)challenged with different S.aureus strains.Differentially expressed(DE)lncRNAs were evaluated,and stable DE lncRNAs were identified in vivo and in vitro.On the basis of the gene sequence conservation and function conservation across species,key lncRNAs with the function of potentially immune regulation were retained for further analysis.The function of FA on inflammation induced by S.aureus challenge was also investigated.Then,the association analysis between these keys lncRNA transcripts and hematological parameters(HPs)was carried out.Lastly,the knockdown and overexpression of the important lncRNA were performed to validate the gene function on the regulation of cell immune response.Results:Linear regression analysis showed a significant correlation between the expression levels of lncRNA shared by mammary tissue and Mac-T cells(P<0.001,R^(2)=0.3517).lncRNAs PRANCR and TNK2-AS1 could be regarded as stable markers associated with bovine S.aureus mastitis.Several HPs could be influenced by SNPs around lncRNAs PRANCR and TNK2-AS1.The results of gene function validation showed PRANCR regulates the mRNA expression of SELPLG and ITGB2 within the S.aureus infection pathway and the Mac-T cells apoptosis.In addition,FA regulated the expression change of DE lncRNA involved in toxin metabolism and inflammation to fight against S.aureus infection.Conclusions:The remarkable association between SNPs around these two lncRNAs and partial HP indicates the potentially important role of PRANCR and TNK2-AS1 in immune regulation.Stable DE lncRNAs PRANCR and TNK2-AS1 can be regarded as potential targets for the prevention of bovine S.aureus mastitis.FA supplementation can reduce the negative effect of S.aureus challenge by regulating the expression of lncRNAs. | Siyuan Mi Yongjie Tang Gerile Dari Yuanjun Shi Jinning Zhang Hailiang Zhang Xueqin Liu Yibing Liu Usman Tahir Ying Yu | 2022 | Journal of Animal Science and Biotechnology2022，13，2： | 2 |
| 2 | PIWIL1 governs the crosstalk of cancer cell metabolism and immunosuppressive microenvironment in hepatocellular carcinoma显示文摘Altered energy metabolism of cancer cells shapes the immune cell response in the tumor microenvironment that faclitates tumorprogression.Herein,we reported the novel of tumor cell-expressed Piwi Like RNA-Mediated Gene Silencing 1(PWL1)in mediatingthe crosstlk of fatty acid metabolism and immune response of human hepatocellular carcinoma(HCC).PlWIL1 expression in HCCwas increased compared to normal hepatic tissues and was positively correlated with the proliferation rate of HCC cell ines.PIWL1overexpression accelerated in vitro proliferation and in vivo growth of HCC tumors,while PIWL1 knockdown showed opositeeffects.PIWL1 increased oxygen consumption and energy production via fatty acid metabolism without altering aerobic glycolysis.lnhibition of fatty acid metablism abolished PWIL1-induced HCC prolferation and growth.RNA-seq analysis revealed that immunesystem regulation might be involved,which was echoed by the experimental observation that PIWL1-overexpressing HCC cellsattracted myeloid-derived suppressor cells(MDSCs)into the tumor microenvironment.MDSCs depletion reduced the prolferationand growth of PlWIL1-overexpressing HCC tumors.Complement C3,whose secretion was induced by PIWL1 in HCC cells,mediatesthe interaction of HCC cells with MDSCs by activated p38 MAPK signaling in MDSCs,which in turn initiated expression of immunosuppressive cytokine L10.Neutralizing lL10 secretion reduced the immunosuppressive activity of MDSCs in the microenvironment of PIWL1-overexpressing HCC.Taken together,our study unraveled the critical role of PIWL1 in initiating theinteraction of cancer cell metabolism and immune cell response in HCC.Tumor cell-expressed PlIWL1 may be a potential target forthe devellopment of novel HCC treatment. | Ning Wang Hor-Yue Tan Yuanjun Lu Yau-Tuen Chan Di Wang Wei Guo Yu Xu Cheng Zhang Feiyu Chen Guoyi Tang Yibin Feng | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 2 |
| 3 | Evaluating the mechanical properties of anisotropic shale containing bedding and natural fractures with discrete element modeling显示文摘Natural fracture data from one of the Carboniferous shale masses in the eastern Qaidam Basin were used to establish a stochastic model of a discrete fracture network and to perform discrete element simulation research on the size efect and mechanical parameters of shale.Analytical solutions of fctitious joints in transversely isotropic media were derived,which made it possible for the proposed numerical model to simulate the bedding and natural fractures in shale masses.The results indicate that there are two main factors infuencing the representative elementary volume(REV)size of a shale mass.The frst and most decisive factor is the presence of natural fractures in the block itself.The second is the anisotropy ratio:the greater the anisotropy is,the larger the REV.The bedding angle has little infuence on the REV size,whereas it has a certain infuence on the mechanical parameters of the rock mass.When the bedding angle approaches the average orientation of the natural fractures,the mechanical parameters of the shale blocks decrease greatly.The REV representing the mechanical properties of the Carboniferous shale masses in the eastern Qaidam Basin were comprehensively identifed by considering the infuence of bedding and natural fractures.When the numerical model size is larger than the REV,the fractured rock mass discontinuities can be transformed into equivalent continuities,which provides a method for simulating shale with natural fractures and bedding to analyze the stability of a borehole wall in shale. | Yingjie Li Lihong Song Yuanjun Tang Jianping Zuo Dongjie Xue | 2022 | International Journal of Coal Science & Technology2022，9，2： | 1 |
| 4 | Environmental consequences of an ultra-low emission retrofit in coal-fired power plants from a life cycle perspective显示文摘To make coal-fired power generation more environmentally friendly,China has initiated a series of ultra-low emission ret-rofits to the air pollution control(APC)system of the existing power plants.In this study,a life cycle assessment(LCA)is conducted to analyze the environmental net benefits for the typical ultra-low emission retrofit of a 1000 MW power plant.The key processes,substances,and APC devices are verified and discussed.The results confirm that the retrofit effectively decreases the environmental stress of acidification potential(AP),eutrophication potential(EP),and photochemical ozone creation potential(POCP)by 69%-79%,which can be attributed to significantly reduced emissions at the stack.However,the retrofit has also increased other impact categories by 24%-79%,primarily due to the consumption of additional elec-tricity and adsorbents.The retrofit of selective catalytic reduction,electrostatic precipitator(ESP),and wet limestone flue gas desulfurization devices has a dominant effect on the impacts of EP,human toxicity potential(HTP),and AP.A newly installed wet ESP shows some environmental benefits(only for AP),but causes considerable burdens,in particular for the investigated impact categories global warming potential(GWP),marine aquatic ecotoxicity(MAETP),and abiotic depletion fossil(ADP fossil).The obtained results indicate that the hidden environmental consequences,which are associated with the production of energy and materials,need to be examined more comprehensively to inform the development of ultra-low emission technologies and strategies effectively. | Jun Dong Yuanjun Tang Aizhong Ruan Jie Chen Xiaoqing Lin Shengyong Lu Xiang Gao | 2021 | Waste Disposal and Sustainable Energy2021，3，4： | 1 |
| 5 | Analysis of parental genetic diversity and its impact on grain yield and quality of japonica hybrid rice in northern China显示文摘The relationship between parental genetic differences and the quality and yield of japonica hybrid rice strongly influences japonica hybrid rice breeding.In this study,137 parental lines of japonica hybrid rice were genotyped using 8 K rice SNP-Chips to characterize their genetic diversity,population structure,and indica-genotype proportion.The genetic diversity of total parental lines averaged 0.264,with values of0.287 for restorer lines and 0.148 for the sterile lines.The introduction of indica lineage increased the genetic diversity of restorer lines relative to that of sterile lines.By model-based population structure analysis,the 137 lines were divided into 14 groups.According to the grouping results,eight restorer lines and five sterile lines were selected from different groups for cross breeding,yielding 40 japonica hybrid rice combinations(F1).Investigation of the yield and quality of these combinations showed that highyield combinations could be obtained by increasing parental genetic distance to 0.8–0.9,a result accomplished largely by the introduction of indica genomic components of restorer lines.To further improve grain quality,the genetic distance between parents should be reduced to 0.4–0.5,suggesting an indicagenotype proportion of 30%–40%for restorer lines.This study may provide a reference for breeding of japonica hybrid rice. | Hong Gao Zuobin Ma Yuanzheng Wang Manli Zhang Xianju Wang Changhua Wang Zhiqiang Tang Liying Zhang Liang Fu Na He Hui Wang Yongan Yin Yuanjun Bai Guomin Sui Wenjing Zheng | 2022 | The Crop Journal2022，10，3： | 1 |
| 6 | Preparation and lithium storage performances of g-C_(3)N_(4)/Si nanocomposites as anode materials for lithium-ion battery显示文摘As the anode material of lithium-ion battery,silicon-based materials have a high theoretical capacity,but their volume changes greatly in the charging and discharging process.To ameliorate the volume expansion issue of silicobased anode materials,g-C_(3)N_(4)/Si nanocomposites are prepared by using the magnesium thermal reduction technique.It is well known that g-C_(3)N_(4)/Si nanocomposites can not only improve the electronic transmission ability,but also ameliorate the physical properties of the material for adapting the stress and strain caused by the volume expansion of silicon in the lithiation and delithiation process.When g-C_(3)N_(4)/Si electrode is evaluated,the initial discharge capacity of g-C_(3)N_(4)/Si nanocomposites is as high as 1033.3 mAh/g at 0.1 A/g,and its reversible capacity is maintained at 548 mAh/g after 400 cycles.Meanwhile,the improved rate capability is achieved with a relatively high reversible specific capacity of 218 mAh/g at 2.0 A/g.The superior lithium storage performances benefit from the unique g-C_(3)N_(4)/Si nanostructure,which improves electroconductivity,reduces volume expansion,and accelerates lithiumion transmission compared to pure silicon. | Zhengxu BIAN Zehua TANG Jinfeng XIE Junhao ZHANG Xingmei GUO Yuanjun LIU Aihua YUAN Feng ZHANG Qinghong KONG | 2020 | Frontiers in Energy2020，14，4： | 1 |
| 7 | AMP-activated protein kinaseβ1 orβ2 deletion enhances colon cancer cell growth and tumorigenesis显示文摘Abnormal metabolism is a major hallmark of cancer and has been validated as a therapeutic target.Adenine monophosphate-activated protein kinase(AMPK),anαβγheterotrimer,performs essential functions in cancer progression due to its central role in maintaining the homeostasis of cellular energy.While the contributions of AMPKαand AMPKγsubunits to cancer development have been established,specific roles of AMPKβ1 and AMPKβ2 isoforms in cancer development are poorly understood.Here,we show the functions of AMPKβ1 and AMPKβ2 in colon cancer.Specifically,deletion of AMPKβ1 or AMPKβ2 leads to increased cell proliferation,colony formation,migration,and tumorigenesis in HCT116 and HT29 colon cancer cells.Interestingly,the AMPKβ1 and AMPKβ2 isoforms have slightly different effects on regulating cancer metabolism,as colon cancer cells with AMPKβ1 knockout showed decreased rates of glycolysis-related oxygen consumption,while AMPKβ2 deletion led to enhanced rates of oxygen consumption due to oxidative phosphorylation.These results demonstrate that functional AMPKβ1 and AMPKβ2 inhibit growth and tumorigenesis in colon cancer cells,suggesting their potential as effective targets for colon cancer therapy. | Fuli Shi Zhimin Tang Shanshan Jiang Zhijuan Xiong Wansi Zhang Yuanjun Li Hui Lin Zhijun Luo Ying Ying | 2022 | Acta Biochimica et Biophysica Sinica2022，54，8： | 0 |
| 8 | Synthesis of Piperazic Acid-Containing Cyclodepsipeptide Core of Verucopeptin显示文摘Comprehensive SummaryChemically,N1 nitrogen of piperazic acid is more nucleophilic than N2 nitrogen,but amide bonds predominantly formed at N2 nitrogen are prevalent in piperazic acid-containing natural products,with only one exception of sanglifehrin.Thus two orthogonal protecting groups of nitrogen are often employed to realize selective coupling of N2 nitrogen,resulting in increased synthetic steps and low synthetic efficiency.However,we develop selective deprotection of N2-Cbz from the N1,N2-diCbz-piperazic acid-containing peptide to form the N2 amide exclusively,avoiding the tedious orthogonal protection strategy which is commonly applied when the easily-accessible N1,N2-diCbz-piperazic acid is used as the synthetic module.We employ this selective-deprotection strategy to achieve an efficient synthesis of piperazic acid-containing cyclodepsipeptide core of verucopeptin with an overall yield of 21%from commercially/readily available building blocks. | Yuanjun Sun Wenhao Tang Mei Wang Huxin Ni Ya-Qiu Long | 2023 | Chinese Journal of Chemistry2023，41，17： | 0 |
| 9 | Reducing children’s exposure to di(2-ethylhexyl)phthalate in homes and kindergartens in China:Impact on lifetime cancer risks and burden of disease显示文摘Exposure to di(2-ethylhexyl)phthalate(DEHP)in the indoor environment has been linked with significant health risks for Chinese children.Multi-phase DEHP concentrations in Chinese residences and kindergartens were estimated using a mass balance model based on the current baseline condition and control strategies(i.e.,increasing ventilation rate,reducing area of sources,using mechanical ventilation systems,and using portable air cleaners).The health benefits of each control strategy were quantified as the reduction in lifetime cancer risks(LCR)and burden of disease(BoD).In the current situation,the mean LCR and disability-adjusted life years(DALY)number attributable to indoor DEHP exposure for Chinese children were around 6.0×10^(−6) and 155 thousand,respectively.The mean LCR and DALY might be reduced by 25%-54%and 16%-40%,respectively,by increasing air exchange rates by 100%,reducing the use of source materials by two-thirds or deploying commercial air cleaners in naturally ventilated buildings.Meanwhile,avoidable DALYs could result in a reduction of mean economic losses of 2.2-5.3 billion RMB.Mechanical ventilation systems with filtration units may not be helpful for reducing children’s health risks.House-specific and tailor-made control measures are critical in lowering indoor exposure to DEHP to promote sustainable buildings and children’s health in China. | Dongsheng Tao Wen Sun Donghui Mo Yonghui Lin Wei Lv Daniel Mmereki Yousheng Xu Yanghui Ye Yuanjun Tang Chao Ye Cong Dong Zhongming Bu | 2024 | Building Simulation2024，17，3： | 0 |
| 10 | 高功率便携式生物质燃烧驱动温差发电机的研发显示文摘利用生物质燃烧产生电力是贫困地区解决照明、通讯和医疗等基本需求的重要方式。本文开发和测试了一种高功率便携式生物质燃烧驱动的温差发电机(biomass-combustion-powered thermoelectric generator,BCP-TEG),详细研究了其温差分布、功率负载特性和不同层级的效率,并开展了现场应用测试。研究结果发现该温差发电机在自身重量为7.6 kg的条件下可同时产生750 W的热力和23.4 W的电力,热电联供效率达到32.3%。温差发电机的发电净功率密度为2.41 W·kg^(-1),均高于所有基于闭式循环冷却的文献报道结果。此外,利用本文开发的温差发电机燃烧1 kg的木条所发出的电能可充满1个容量为6.2 A·h的3.7 V锂电池。最后,论文详细讨论了BCP-TEG研究领域存在的问题和未来可研究的方向。 | Guoneng Li Jie Ying Minbo Yi Youqu Zheng Yuanjun Tang Wenwen Guo | 2023 | Engineering2023，，5： | 0 |
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