|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration显示文摘The capacity of 3D organoids to mimic physiological tissue organization and functionality has provided an invaluable tool to model development and disease in vitro.However,conventional organoid cultures primarily represent the homeostasis of self-organizing stem cells and their derivatives.Here,we established a novel intestinal organoid culture system composed of 8 components,mainly including VPA,EPZ6438,LDN193189,and R-Spondin 1 conditioned medium,which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury;therefore,these organoids were designated hyperplastic intestinal organoids(Hyper-organoids).Single-cell RNA sequencing identified different regenerative stem cell populations in our Hyper-organoids that shared molecular features with in vivo injury-responsive Lgr5+stem cells or Clu+revival stem cells.Further analysis revealed that VPA and EPZ6438 were indispensable for epigenome reprogramming and regeneration in Hyper-organoids,which functioned through epigenetically regulating YAP signaling.Furthermore,VPA and EPZ6438 synergistically promoted regenerative response in gut upon damage in vivo.In summary,our results demonstrated a new in vitro organoid model to study epithelial regeneration,highlighting the importance of epigenetic reprogramming that pioneers tissue repair. | Molong Qu Liang Xiong Yulin Lyu Xiannian Zhang Jie Shen Jingyang Guan Peiyuan Chai Zhongqing Lin Boyao Nie Cheng Li Jun Xu Hongkui Deng | 2021 | Cell Research2021，31，3： | 9 |
| 2 | Generation of human hepatocytes from extended pluripotent stem cells显示文摘Dear Editor,Human pluripotent stem cells have great potential for application in regenerative medicine,as they permit the generation of different lineages of functional cell types via directed differentiation.1 However,application of conventional human pluripotent stem cells is limited due to several factors,including heterogeneity in pluripotency,differentiation bias,relatively slow proliferation,and poor single-cell survival.2 Toovercome these problems. | Qiming Wang Da Sun Zhen Liang Junyi Wang Xinxing Zhong Yulin Lyu Junning Cao Zhongqing Lin Yuanyuan Du Zhenchuan Miao Shichun Lu Cheng Li Jun Xu Yan Shi Hongkui Deng | 2020 | Cell Research2020，30，9： | 1 |
| 3 | Derivation of totipotent-like stem cells with blastocyst-like structure forming potential显示文摘It is challenging to derive totipotent stem cells in vitro that functionally and molecularly resemble cells from totipotent embryos.Here,we report that a chemical cocktail enables the derivation of totipotent-like stem cells,designated as totipotent potential stem(TPS)cells,from 2-cell mouse embryos and extended pluripotent stem cells,and that these TPS cells can be stably maintained long term in vitro.TPS cells shared features with 2-cell mouse embryos in terms of totipotency markers,transcriptome,chromatin accessibility and DNA methylation patterns.In vivo chimera formation assays show that these cells have embryonic and extraembryonic developmental potentials at the single-cell level.Moreover,TPS cells can be induced into blastocyst-like structures resembling preimplantation mouse blastocysts.Mechanistically,inhibition of HDAC1/2 and DOT1L activity and activation of RARγsignaling are important for inducing and maintaining totipotent features of TPS cells.Our study opens up a new path toward fully capturing totipotent stem cells in vitro. | Yaxing Xu Jingru Zhao Yixuan Ren Xuyang Wang Yulin Lyu Bingqing Xie Yiming Sun Xiandun Yuan Haiyin Liu Weifeng Yang Yenan Fu Yu Yu Yinan Liu Rong Mu Cheng Li Jun Xu Hongkui Deng | 2022 | Cell Research2022，32，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员