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| 1 | Downregulation of SNRPG induces cell cycle arrest and sensitizes human glioblastoma cells to temozolomide by targeting Myc through a p53-dependent signaling pathway显示文摘Objective:Temozolomide(TMZ)is commonly used for glioblastoma multiforme(GBM)chemotherapy.However,drug resistance limits its therapeutic effect in GBM treatment.RNA-binding proteins(RBPs)have vital roles in posttranscriptional events.While disturbance of RBP-RNA network activity is potentially associated with cancer development,the precise mechanisms are not fully known.The SNRPG gene,encoding small nuclear ribonucleoprotein polypeptide G,was recently found to be related to cancer incidence,but its exact function has yet to be elucidated.Methods:SNRPG knockdown was achieved via short hairpin RNAs.Gene expression profiling and Western blot analyses were used to identify potential glioma cell growth signaling pathways affected by SNRPG.Xenograft tumors were examined to determine the carcinogenic effects of SNRPG on glioma tissues.Results:The SNRPG-mediated inhibitory effect on glioma cells might be due to the targeted prevention of Myc and p53.In addition,the effects of SNRPG loss on p53 levels and cell cycle progression were found to be Myc-dependent.Furthermore,SNRPG was increased in TMZ-resistant GBM cells,and downregulation of SNRPG potentially sensitized resistant cells to TMZ,suggesting that SNRPG deficiency decreases the chemoresistance of GBM cells to TMZ via the p53 signaling pathway.Our data confirmed that SNRPG suppression sensitizes GBM cells to TMZ by targeting Myc via the p53 signaling cascade.Conclusions:These results indicated that SNRPG is a probable molecular target of GBM and suggested that suppressing SNRPG in resistant GBM cells might be a substantially beneficial method for overcoming essential drug resistance. | Yulong Lan Jiacheng Lou Jiliang Hu Zhikuan Yu Wen Lyu Bo Zhang | 2020 | Cancer Biology & Medicine2020，17，1： | 3 |
| 2 | Influence of oxygen content on the microstructure and mechanical properties of cold rolled Ti-32.5Nb-6.8Zr-2.7Sn-x O alloys after aging treatment显示文摘The influence of oxygen(O) content on the microstructure and mechanical properties of cold rolled Ti-32.5 Nb-6.8 Zr-2.7 Sn-xO(TNZS-xO; x = 0,0.3.0.6; mass%) alloys after aging at temperature range from 350 to 600℃ for 24 h was investigated. Results showed that the cold rolled TNZS-xO alloys possess single βphase. During the aging process, O could not only effectively suppress the precipitation of ω phase but also retard the formation and decomposition of a phase. In addition, the corresponding temperatures of the maximal volume fraction of α phase precipitation and the(α+β)/β transus temperatures of the TNZS-xO alloys were all increased with the increasing of O content. For mechanical properties, it was found that the strength and Young's modulus of the TNZS-xO alloys increased owing to the ω phase and/or a phase precipitation and decreased owing to the a phase decomposition. However, the elongation showed the opposite change tendency with the above mentioned strength. The mechanical properties of TNZS-xO alloys can be controlled over a wide range by subjecting to aging treatment and/or changing their 0 content. When aged at or below 450 ℃,the TNZS-xO alloys exhibit great potential to become a series of new candidates for biomedical applications since they possess high strength(870-1460 MPa), low Young's modulus(45.1-75.6 GPa), high strength-to-modulus ratio(0.018-0.02) and appropriate elongation(7.2%-14.9%), which are superior to those of Ti-6 Al-4 V alloy and suitable for the use as bio-implant materials. | Chunbo Lan Feng Chen Huijuan Chen Yulong Wu Xiangwei Wu | 2018 | Journal of Materials Science & Technology2018，34，11： | 2 |
| 3 | First experimental comparisons of laser-plasma interactions between spherical and cylindrical hohlraums at SGⅢ laser facility显示文摘We present our recent laser-plasmas instability(LPI)comparison experiment at the SGIII laser facility between the spherical and cylindrical hohlraums.Three kinds of filling are considered:vacuum,gas-filling with or without a capsule inside.A spherical hohlraum of 3.6 mm in diameter,and a cylindrical hohlraum of 2.4 mm?4.3 mm are used.The capsule diameter is 0.96 mm.A flat-top laser pulse with 3 ns duration and up to 92.73 kJ energy is used.The experiment has shown that the LPI level in the spherical hohlraum is close to that of the outer beam in the cylindrical hohlraum,while much lower than that of the inner beam.The experiment is further simulated by using our 2-dimensional radiation hydrodynamic code LARED-Integration,and the laser back-scattering fraction and the stimulated Raman scatter(SRS)spectrum are post-processed by the high efficiency code of laser interaction with plasmas HLIP.According to the simulation,the plasma waves are strongly damped and the SRS is mainly developed at the plasma conditions of electron density from 0.08 n_(c) to 0.1 n_(c) and electron temperature from 1.5 keV to 2.0 keV inside the hohlraums.However,obvious differences between the simulation and experiment are found,such as that the SRS back-scattering is underestimated,and the numerical SRS spectrum peaks at a larger wavelength and at a later time than the data.These dif-ferences indicate that the development of a 3D radiation hydrodynamic code,with more accurate physics models,is mandatory for spherical hohlraum study. | Yaohua Chen Zhichao Li Xufei Xie Chunyang Zheng Chuanlei Zhai Liang Hao Dong Yang Wenyi Huo Guoli Ren Jie Liu Xiaoshi Peng Tao Xu Yulong Li Sanwei Li Zhiwen Yang Liang Guo Lifei Hou Yonggang Liu Huiyue Wei Xiangming Liu Weiyi Cha Yukun Li Keli Deng Zheng Yuan Xiayu Zhan Haijun Zhang Baibin Jiang Wei Zhang Kai Du Xuewei Deng Yongkun Ding Xiaofeng Wei Wanguo Zheng Xiaodong Chen Xiantu He Ke Lan | 2017 | Matter and Radiation at Extremes2017，2，2： | 0 |
| 4 | Determination of laser entrance hole size for ignition-scale octahedral spherical hohlraums显示文摘A recently proposed octahedral spherical hohlraum with six laser entrance holes(LEHs)is an attractive concept for an upgraded laser facility aiming at a predictable and reproducible fusion gain with a simple target design.However,with the laser energies available at present,LEH size can be a critical issue.Owing to the uncertainties in simulation results,the LEH size should be determined on the basis of experimental evidence.However,determination of LEH size of an ignition target at a small-scale laser facility poses difficulties.In this paper,we propose to use the prepulse of an ignition pulse to determine the LEH size for ignition-scale hohlraums via LEH closure behavior,and we present convincing evidence from multiple diagnostics at the SGIII facility with ignition-scale hohlraum,laser prepulse,and laser beam size.The LEH closure observed in our experiment is in agreement with data from the National Ignition Facility.The total LEH area of the octahedral hohlraum is found to be very close to that of a cylindrical hohlraum,thus successfully demonstrating the feasibility of the octahedral hohlraum in terms of laser energy,which is crucially important for sizing an ignition-scale octahedrally configured laser system.This work provides a novel way to determine the LEH size of an ignition target at a small-scale laser facility,and it can be applied to other hohlraum configurations for the indirect drive approach. | Yao-Hua Chen Zhichao Li Hui Cao Kaiqiang Pan Sanwei Li Xufei Xie Bo Deng Qiangqiang Wang Zhurong Cao Lifei Hou Xingsen Che Pin Yang Yingjie Li Xiaoan He Tao Xu Yonggang Liu Yulong Li Xiangming Liu Haijun Zhang Wei Zhang Baibin Jiang Jun Xie Wei Zhou Xiaoxia Huang Wen Yi Huo Guoli Ren Kai Li Xudeng Hang Shu Li Chuanlei Zhai Jie Liu Shiyang Zou Yongkun Ding Ke Lan | 2022 | Matter and Radiation at Extremes2022，7，6： | 0 |
| 5 | Young Leaf White Stripe encodes a P-type PPR protein required for chloroplast development显示文摘Pentatricopeptide repeat(PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR proteins are known to function in chloroplast development in rice(Oryza sativa), the detailed molecular functions of many PPR proteins remain unclear.Here, we characterized a rice young leaf white stripe(ylws) mutant, which has defective chloroplast development during early seedling growth.Map-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted PPR protein with 11 PPR motifs. Further expression analyses showed that many nuclear-and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2,and rps12, and editing of ndhA, ndhB, and rps14transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development. | Jie Lan Qibing Lin Chunlei Zhou Xi Liu Rong Miao Tengfei Ma Yaping Chen Changling Mou Ruonan Jing Miao Feng Thanhliem Nguyen Yulong Ren Zhijun Cheng Xin Zhang Shijia Liu Ling Jiang Jianmin Wan | 2023 | Journal of Integrative Plant Biology2023，65，7： | 0 |
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