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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Prevalence and evolution of drug resistance HIV-1 variants in Henan, China显示文摘To understand the prevalence and evolution of drug resistant HIV strains in Henan China after the implementation of free antiretroviral therapy for AIDS patients. 45 drug nave AIDS patients, 118 AIDS patients who received three months antiretroviral therapy and 124 AIDS patients who received six months antiretroviral treatment were recruited in the southern part of Henan province. Information on general condition, antiretroviral medicines, adherence and clinical syndromes were collected by face to face interview. Meanwhile, 14ml EDTA anticoagulant blood was drawn. CD4/CD8 T cell count, viral load and genotypic drug resistance were tested. The rates of clinical improvement were 55.1% and 50.8% respectively three months and six months after antiretroviral therapy. The mean CD4 cell count after antiretroviral therapy was significantly higher than in drug nave patients. The prevalence rate of drug resistant HIV strains were 13. 9%, 45.4% and 62.7% in drug nave patients, three month treatment patients and six month treatment patients, respectively. The number of resistance mutation codons and the frequency of mutations increased significantly with continued antiretroviral therapy. The mutation sites were primarily at the 103, 106 and 215 codons in the three-month treatment group and they increased to 15 codon mutations in the six-month treatment group. From this result, the evolution of drug resistant strains was inferred to begin with the high level NNRTI resistant strain, and then develop low level resistant strains to NRTIs. The HIV strains with high level resistance to NVP and low level resistance to AZT and DDI were highly prevalent because of the AZT+DDI+NVP combination therapy. These HIV strains were also cross resistant to DLV, EFV, DDC and D4T. Poor adherence to therapy was believed to be the main reason for the emergence and prevalence of drug resistant HIV strains. The prevalence of drug resistant HIV strains was increased with the continu- ation of antiretroviral therapy in the southern part of Henan province. Measures, that could promote high level adherence, provide new drugs and change ART regimens in failing patients, should be implemented as soon as possible. | Jing Yun LI Han Ping LI Lin LI Hong LI Zhe WANG Kun YANG Zuo Yi BAO Dao Min ZHUANG Si Yang LIU Yong Jian LIU Hui XING Yi Ming SHAO | 2005 | Cell Research2005，15，11： | 34 |
| 3 | Clinical and laboratory survey of 65 Chinese patients with Leigh syndrome显示文摘Background Leigh syndrome is an inherited neurodegenerative disease that emerges in infancy and childhood and presents with a clinically heterogeneous variety of neuromuscular and non-neuromuscular disorders. It can result from the inheritance of mutations in either nuclear or mitochondrial DNA. In the current study, we performed a retrospective study in 65 patients in order to investigate the clinical and genetic characteristics of Leigh syndrome in Chinese patients. Methods Sixty-five unrelated cases (35 men and 30 women) who were hospitalized in the past 12 years were reviewed. Diagnosis was based on both the clinical presentation and the characteristic neuropathologic findings of bilateral symmetric necrotizing lesions in the basal ganglia and brain stem as detected using cranial computed tomography (CT) scan or magnetic resonance imaging (MRI). The differential diagnosis of organic acidurias and fatty acid ?-oxidation defects were performed. Specific point mutations and deletions in mitochondrial DNA (T8993G, T8993C, T9176C, A8344G, A3243G) were screened by PCR-restriction analysis and Southern blot. The SURF1 gene was sequenced. Skeletal muscle biopsies were performed in 17 (26.2%) of the patients. The diagnosis was confirmed by autopsy in 6 (9.2%) patients. Results The patients had various forms of metabolic encephalomyopathy. Fifty-nine (90.8%) of the patients had the typical neuroradiological features of Leigh syndrome, including symmetrical necrotizing lesions scattered within the basal ganglia, thalamus and brain stem. Twenty (30.8%) patients were confirmed by genetic, biochemical analysis and autopsy. Specific point mutations in mitochondrial DNA were found in 5 cases (7.7%). Of these, the A8344G mutation was detected in 2 patients. The T8993G, T8993C, and A3243G point mutations were identified in 3 other patients, respectively. SURF1 mutations associated with cytochrome c oxidase deficiency were identified in 8 (12.3%) families by DNA sequencing. A G604C mutation was identified in 6 (9.2%) patients. The genotypes of 52 patients remained unknown. Conclusions Leigh syndrome presents as a diverse array of clinical features and can result from specific mutations in nuclear or mitochondrial DNA. In this study, SURF1 mutations associated with cytochrome c oxidase deficiency were identified in 8 (12.3%) out of 65 patients with Leigh syndrome. It indicates that SURF1 mutations might be a common cause of Leigh syndrome in China. The etiology of Leigh syndrome in Chinese patients represents a persistent challenge to clinicians. | YANG Yan-ling SUN Fang ZHANG Yao QIAN Ning YUAN Yun WANG Zhao-xia QI Yu XIAO Jiang-xi WANG Xiao-ying QI Zhao-yue ZHANG Yue-hua JIANG Yu-wu BAO Xin-hua QIN Jiong WU Xi-ru | 2006 | Chinese Medical Journal2006，，5： | 17 |
| 4 | A cytotoxic compound from the leaves of Juglans mandshurica显示文摘From Juglans mandshurica leaves, a new quinone compound was isolated through bioassay-guided fractionation. The structure elucidation of the compound was established based on spectroscopic studies, notably of the 2D NMR spectra. The compound exhibited moderate cytotoxic activities against Hela, MCF-7, BGC823 and 3T3-L1cell lines with IC50 ranges from 7.5 to26.8 mmol/L. | Zhi Bo Li Yong Ming Bao Hong Bo Chen Jing Yun Wang Jie Han Hu Li Jia An | 2007 | Chinese Chemical Letters2007，18，7： | 11 |
| 5 | Toripalimab plus chemotherapy as second-line treatment in previously EGFR-TKI treated patients with EGFR-mutant-advanced NSCLC:a multicenter phase-II trial显示文摘This multicenter phase-II trial aimed to investigate the efficacy,safety,and predictive biomarkers of toripalimab plus chemotherapy as second-line treatment in patients with EGFR-mutant-advanced NSCLC.Patients who failed from first-line EGFR-TKIs and did not harbor T790M mutation were enrolled.Toripalimab plus carboplatin and pemetrexed were administrated every three weeks for up to six cycles,followed by the maintenance of toripalimab and pemetrexed.The primary endpoint was objective-response rate(ORR).Integrated biomarker analysis of PD-L1 expression,tumor mutational burden(TMB),CD8+tumor-infiltrating lymphocyte(TIL)density,whole-exome,and transcriptome sequencing on tumor biopsies were also conducted.Forty patients were enrolled with an overall ORR of 50.0%and disease-control rate(DCR)of 87.5%.The median progression free survival(PFS)and overall survival were 7.0 and 23.5 months,respectively.The most common treatment-related adverse effects were leukopenia,neutropenia,anemia,ALT/AST elevation,and nausea.Biomarker analysis showed that none of PD-L1 expression,TMB level,and CD8+TIL density could serve as a predictive biomarker.Integrated analysis of whole-exome and transcriptome sequencing data revealed that patients with DSPP mutation had a decreased M2 macrophage infiltration and associated with longer PFS than those of wild type.Toripalimab plus chemotherapy showed a promising anti-tumor activity with acceptable safety profiles as the second-line setting in patients with EGFR-mutant NSCLC.DSPP mutation might serve as a potential biomarker for this combination.A phase-III trial to compare toripalimab versus placebo in combination with chemotherapy in this setting is ongoing(NCT03924050). | Tao Jiang Pingyang Wang Jie Zhang Yanqiu Zhao Jianying Zhou Yun Fan Yongqian Shu Xiaoqing Liu Helong Zhang Jianxing He Guanghui Gao Xiaoqian Mu Zhang Bao Yanjun Xu Renhua Guo Hong Wang Lin Deng Ningqiang Ma Yalei Zhang Hui Feng Sheng Yao Jiarui Wu Luonan Chen Caicun Zhou Shengxiang Ren | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 8 |
| 6 | (x-Helical domain is essential for antimicrobial activity of high mobility group nucleosomal binding domain 2 (HMGN2)显示文摘Aim:To examine the antimicrobial spectrum and functional structure of highmobility group nucleosomal binding domain 2(HMGN2).Methods:OMIGA pro-tein structure software was used to analyze the two-dimensional structure ofHMGN2.Synthetic short peptides were generated for studying the relationshipbetween function and structure.Prokaryotic expression vectors were constructedfor the holo-HMGN2 and its helical domain.Their E coil-based products werealso prepared for antimicrobial testing.The antimicrobial assay included minimaleffective concentration,minimal inhibitory concentration,and minimal bacteri-cidal concentration.Results:OMIGA protein structure software analysis revealeda transmembrane or-helical structure(the putative antimicrobial domain)locatedfrom position 18 to 48 of the HMGN2 protein sequence.The antimicrobial assayshowed that the MIC of the recombinant holo-HMGN2 against E coli ML-35p(anampicillin-resistance strain),Pseudomonas aeruginosa ATCC 27853 and Candidaalbicans ATCC 10231 were 12.5,25,and 100 rag/L,respectively.Against the samemicroorganisms,the MIC of the synthetic HMGN2 α-helical domain were 12.5,25,and 100 mg/L,respectively,that is,the same as with the recombinant form ofHMGN2.In contrast,recombinant holo-HMGN2 was inactive against Staphylo-coccus aureus ATCC 25923.The synthetic N-terminal and C-terminal fragments ofHMGN2 had no antimicrobial activity against E coli ML-35p,P aeruginosa ATCC27853 or C albicans ATCC 10231.Conclusion:HMGN2 showed potent antimi-crobial activity against E coli ML-35p,P aeruginosa ATCC 27853 and,to someextent,against C albicans ATCC 10231,but was inactive against S aureus ATCC25923 in these assay systems.Its α-helical structure may be essential for theantimicrobial activity of HMGN2. | Yun FEN Ning HUANG Qi WU Lang BAO Bo-yao WANG | 2005 | Acta Pharmacologica Sinica2005，26，9： | 8 |
| 7 | Endothelial cell-specific anticoagulation reduces inflammation in a mouse model of acute lung injury显示文摘Tissue factor (TF)-dependent coagulation contributes to lung inflammation and the pathogenesis of acute lung injury (ALI). In this study, we explored the roles of targeted endothelial anticoagulation in ALI using two strains of transgenic mice expressing either a membrane-tethered human tissue factor pathway inhibitor (hTFPI) or hirudin fusion protein on CD31^+ cells, including vascular endothelial cells (ECs). ALI was induced by intratracheal injection of LPS, and after 24 h the expression of TF and protease-activated receptors (PARs) on EC in lungs were assessed, alongside the extent of inflammation and injury. The expression of TF and PARs on the EC in lungs was upregulated after ALI. In the two strains of transgenic mice, expression of either of hTFPI or hirudin by EC was associated with significant reduction of inflammation, as assessed by the extent of leukocyte infiltration or the levels of proinflammatory cytokines, and promoted survival after LPS-induced ALI. The beneficial outcomes were associated with inhibition of the expression of chemokine CCL2 in lung tissues. The protection observed in the CD31-TFPI-transgenic strain was abolished by injection of an anti-hTFPI antibody, but not by prior engraftment of the transgenic strains with WT bone marrow, confirming that the changes observed were a specific transgenic expression of anticoagulants by EC. These results demonstrate that the inflammation in ALI is TF and thrombin dependent, and that expression of anticoagulants by EC significantly inhibits the development of ALI via repression of leukocyte infiltration, most likely via inhibition of chemokine gradients. These data enhance our understanding of the pathology of ALI and suggest a novel therapeutic strategy for treatment. | Jian Lou Yue Hu Min-dan Wu Luan-qing Che Yin-fang Wu Yun Zhao Bao-ping Tian Zheng-qiang Bao Chen Zhu Yan-ping Wu Lu-lu He Chun-xue Bai Jian Zhou Song-min Ying Wen Li Zhi-hua Chen Da-xin Chen Anthony Dorling Hua-hao Shen | 2019 | Acta Pharmacologica Sinica2019，40，6： | 7 |
| 8 | Projection of China's Near- and Long-Term Climate in a New High-Resolution Daily Downscaled Dataset NEX-GDDP显示文摘The projection of China's near- and long-term future climate is revisited with a new-generation statistically downscaled dataset, NEX-GDDP(NASA Earth Exchange Global Daily Downscaled Projections). This dataset presents a high-resolution seamless climate projection from 1950 to 2100 by combining observations and GCM results, and remarkably improves CMIP5 hindcasts and projections from large scale to regional-to-local scales with an unchanged long-term trend. Three aspects are significantly improved:(1) the climatology in the past as compared against the observations;(2) more reliable near- and long-term projections, with a modified range of absolute value and reduced inter-model spread as compared to CMIP5 GCMs; and(3) much added value at regional-to-local scales compared to GCM outputs. NEX-GDDP has great potential to become a widely-used high-resolution dataset and a benchmark of modern climate change for diverse earth science communities. | Yun BAO Xinyu WEN | 2017 | Journal of Meteorological Research2017，31，1： | 7 |
| 9 | Paeonivayin, A New Monoterpene Glycoside from Paeonia delavayi显示文摘A new monoterpenc glycoside named paeonivayin with other seven known compounds were isolated from the roots of Paeonia delavayi Franch, and their structures were determined bymeans of spectroscopic studies. | Yun Bao MA Da Gang WU Ji Kai LIU (Department of Phytochemistry,Kunming Institute of Botany.The Chinese Academy of Sciences.Kunming 650204) | 1999 | Chinese Chemical Letters1999，10，9： | 7 |
| 10 | Endocrine Disruption Activity of 30-day Dietary Exposure to Decabromodiphenyl Ethane in Balb/C Mouse显示文摘Objective This study aimed to evaluate the hepatotoxicity,metabolic disturbance activity and endocrine disrupting activity of mice treated by Decabromodiphenyl ethane(DBDPE).Methods In this study,Balb/C mice were treated orally by gavage with various doses of DBDPE.After 30 days of treatment,mice were sacrificed; blood,livers and thyroid glands were obtained,and hepatic microsomes were isolated.Biochemical parameters including 8 clinical chemistry parameters,blood glucose and hormone levels including insulin and thyroid hormone were assayed.The effects of DBDPE on hepatic cytochrome P450(CYP) levels and activities and uridinediphosphate-glucuronosyltransferase(UDPGT) activities were investigated.Liver and thyroid glands were observed.Results There were no obvious signs of toxicity and no significant treatment effect on body weight,or liver-to-body weight ratios between treatment groups.The levels of ALT and AST of higher dose treatment groups were markedly increased.Blood glucose levels of treatment groups were higher than those of control group.There was also an induction in TSH,T3,and f T3.UDPGT,PROD,and EROD activities were found to have been increased significantly in the high dose group.Histopathologic liver changes were characterized by hepatocyte hypertrophy and cytoplasmic vacuolization.Our findings suggest that DBDPE can cause a certain degree of mouse liver damage and insufficiency.Conclusion DBDPE has the activity of endocrine disruptors in Bal/C mice,which may induce drug-metabolizing enzymes including CYPs and UDPGT,and interfere with thyroid hormone levels mediated by Ah R and CAR signaling pathways.Endocrine disrupting activity of DBDPE could also affect the glucose metabolism homeostasis. | SUN Ru Bao SHANG Shuai ZHANG Wei LIN Ben Cheng WANG Qiang SHI Yun XI Zhu Ge | 2018 | Biomedical and Environmental Sciences2018，31，1： | 7 |
| 11 | A Multicenter Study of Viral Aetiology of Community-Acquired Pneumonia in Hospitalized Children in Chinese Mainland显示文摘Community-acquired pneumonia(CAP) is one of the leading causes of morbidity and mortality in children worldwide.In this study,we aimed to describe the aetiology of viral infection of pediatric CAP in Chinese mainland.During November2014 to June 2016,the prospective study was conducted in 13 hospitals.The hospitalized children under 18 years old who met the criteria for CAP were enrolled.The throat swabs or nasopharyngeal aspirates(NPAs) were collected which were then screened 18 respiratory viruses using multiplex PCR assay.Viral pathogens were present in 56.6%(1539/2721) of the enrolled cases,with the detection rate of single virus in 39.8% of the cases and multiple viruses in 16.8% of the cases.The most frequently detected virus was respiratory syncytial virus(RSV)(15.2%,414/2721).The highest detection rate of virus was in <6-month-age group(70.7%,292/413).RSV,human metapneumovirus(HMPV),human parainfluenza viruses(HPIVs) and influenza B virus(Flu B) showed the similar prevalence patterns both in north and south China,but HPIVs,Flu A,human bocavirus(HBoV),human adenovirus(HAdV) and human coronaviruses(HCoVs) showed the distinct circulating patterns in north and south China.Human enterovirus/human rhinovirus(HEV/HRV)(27.6%,27/98),HBoV(18.4%,18/98),RSV(16.3%,16/98) and HMPV(14.3%,14/98) were the most commonly detected viruses in severe pneumonia cases with single virus infection.In conclusion,viral pathogens are frequently detected in pediatric CAP cases and may therefore play a vital role in the aetiology of CAP.RSV was the most important virus in hospitalized children with CAP in Chinese mainland. | Yun Zhu Baoping Xu Changchong Li Zhimin Chen Ling Cao Zhou Fu Yunxiao Shang Aihuan Chen Li Deng Yixiao Bao Yun Sun Limin Ning Shuilian Yu Fang Gu Chunyan Liu Ju Yin Adong Shen Zhengde Xie Kunling Shen | 2021 | Virologica Sinica2021，36，6： | 7 |
| 12 | Advances and Prospects of Surface Modification on Nickel-Rich Materials for Lithium-Ion Batteries显示文摘Although layered Ni-rich cathode materials have attracted lots of attention for their high capacity and power density,several significant issues,such aspoor thermal stability and moderate oyclability lit their practical appications.Most of these undesired problems of Ni-rich materials are caused by theunstable surface or the parasic reactions at cathode-electrolyte interface.5urface coating is the most common method to suppress such interfacialproblems for Ni-rich materials.This review focuses on the surface engineering of the N-rich materials in recent years,including the species used in coat.ing synthetic strategies of uniform coating layer,and the positive effects of coating species on the active materials.Detailed discussions are also taken todescribe the formation mechanism of the surface coating layer with design philosophy.Finally,the prospects for further developments and challenges insurface coating are also summarized. | Yuefeng Su Gang Chen Lai Chen Qing Li Yun Lu Liying Bao Ning Li Shi Chen Feng Wu | 2020 | Chinese Journal of Chemistry2020，38，12： | 5 |
| 13 | The mechanism of side reaction induced capacity fading of Ni-rich cathode materials for lithium ion batteries显示文摘Ni-rich cathode materials show great potential of applying in high-energy lithium ion batteries,but their inferior cycling stability hinders this process.Study on the electrode/electrolyte interfacial reaction is indispensable to understand the capacity failure mechanism of Ni-rich cathode materials and further address this issue.This work demonstrates the domain size effects on interfacial side reactions firstly,and further analyzes the inherent mechanism of side reaction induced capacity decay through comparing the interfacial behaviors before and after MgO coating.It has been determined that LiF deposition caused thicker SEI films may not increase the surface film resistance,while HF erosion induced surface phase transition will increase the charge transfer resistance,and the later plays the dominant factor to declined capacity of Ni-rich cathode materials.This work suggests strategies to suppress the capacity decay of layered cathode materials and provides a guidance for the domain size control to match the various applications under different current rates. | Daozhong Hu Yuefeng Su Lai Chen Ning Li Liying Bao Yun Lu Qiyu Zhang Jing Wang Shi Chen Feng Wu | 2021 | Journal of Energy Chemistry2021，30，7： | 5 |
| 14 | Photoluminescence and defect evolution of nano-ZnO thin films at low temperature annealing显示文摘Nano-ZnO thin films composed of nanoparticles with sizes of 10-16 nm on silicon substrates at low temperature were prepared by sol-gel method.By placing the nano-ZnO thin films at room temperature or annealing at 100°C in air for 10 h intermittently,within a total 70 h annealing time,the evolution of PL spectra of the nano-ZnO thin films were studied in detail.As the annealing time increases,the PL peaks shift from violet to blue and green bands.The PL peaks at violet and blue bands decrease with the annealing time,but the PL peaks at green band are opposite.The PL spectra are related to the defects in the nano-ZnO thin films.The PL peaks positioned at 430 nm are mainly related to defects of zinc interstatials(Zni),oxygen vacancies and(Vo);the ones at 420 nm to oxygen interstitials(Oi),Zinc vacancies(Vzn),Zni ;and the ones at 468 nm to Vzn,Zni,and charged oxygen interstatials(Vo+).The green luminescence is related to Oi,Vo and Zni.The evolutions of PL spectra and the defects are also related to the concentrations of Zn in the thin films,the thicknesses of the films and the annealing time.For the films with 0.5 M and 1.0 M Zn concentrations,after 20 h and 30 h annealing in air at 100°C,respectively,either placing them in air at room temperature or continuing anneal in air at 100°C,the PL spectra are stable.Under the low temperature annealing,Zni decreases with the annealing time,and Oi increases.Sufficient Oi favors to keep the nano-ZnO thin films stable.This result is important to nano-ZnO thin films as electron transport layers in inverted or tandem organic solar cells. | YANG AiLing YANG Yun ZHANG ZhenZhen BAO XiChang YANG RenQiang LI ShunPin SUN Liang | 2013 | Science China(Technological Sciences)2013，56，1： | 5 |
| 15 | Preparation and characterizations of helical mesoporous silica nanorods using CTAB and alcohols显示文摘螺旋状的 mesoporous 硅石 nanorods 被准备把 cetyltrimethylammonium 溴化物和 achiral 白酒用作 co-structure-directing 代理人。他们用扫描电子显微镜学,传播电子显微镜学,氮吸着,和小角度 X 光检查衍射的地排放被描绘。硅石 nanorods 的长度与增加白酒的长度增加。当 n-heptanol 和 n-octanol 被使用时,有表面上的薄片状的 mesopores 的螺旋状的 mesoporous 硅石 nanorods 被获得。 | Yun Yue Pu Yi Li Wei Zhuang Ming Zhang Bao Zong Li Yong Gang Yang | 2012 | Chinese Chemical Letters2012，23，10： | 5 |
| 16 | Comparison of Two Molecular Assays For Detecting Smear Negative Pulmonary Tuberculosis显示文摘Objective To compare the performance of MTBDRplus V2 and Xpert MTB/RIF for detecting smear negative pulmonary tuberculosis(PTB). Methods Clinical PTB suspects were enrolled consecutively in Anhui Chest Hospital and Xi'an Chest Hospital from January to December in 2014. The sputum samples of smear negative PTB suspects were collected and decontaminated. The sediment was used to conduct MTBDRplus V2, Xpert MTB/RIF and drug susceptibility test(DST). All the samples with discrepant drug susceptibility result between molecular methods and phenotypic method were confirmed by DNA sequencing. Results A total of 1973 cases were enrolled in this study. The detection rates of Mycobacterium tuberculosis complex(MTBC) by MTBDRplus V2 and Xpert MTB/RIF were 27.67% and 27.98%, respectively. When setting MGIT culture result as a gold standard, the sensitivity and specificity of MTBDRplus V2 were 86.74% and 93.84%, and the sensitivity and specificity of Xpert MTB/RIF were 86.55% and 93.43%, respectively. For the detection of the resistance to rifampin, the sensitivity and specificity of MTBDRplus V2 were 94.34% and 96.62%, and the sensitivity and specificity of Xpert MTB/RIF were 88.68% and 95.96%, respectively. For the detection of the resistance to isoniazid, the sensitivity and specificity of MTBDRplus V2 were 77.38% and 98.02%, respectively. Conclusion MTBDRplus V2 and Xpert MTB/RIF can be used to detect MTBC in smear negative samples with satisfactory performance. | LI Qiang BAO Xun Di LIU Yun OU Xi Chao PANG Yu ZHAO Yan Lin | 2016 | Biomedical and Environmental Sciences2016，29，4： | 5 |
| 17 | A New 24, 30-Dinortriterpenoid from Paeonia delavayi显示文摘A new triterpenoid, 3(, 4(, 23-trihydroxy-24, 30-dinorolean-12, 20(29)-dien-28-oic acid, together with five known compounds, 2(, 3(, 23-trihydroxy-12-oleanen-28-oic acid-(-D-glucopyranosyl ester, palbinone, 2-hydroxy-benzoic acid, vanillic acid, syringic acid, were isolated from the roots of Paeonia delavayi Franch. Their structures were characterized by spectral analysis. | Shao Hua WU, Xiao Dong LUO, Yun Bao MA, Xiao Jiang HAO, Da Gang WU* Laboratory of Phytochemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 | 2001 | Chinese Chemical Letters2001，12，4： | 5 |
| 18 | Levetiracetam administration is correlated with lower mortality in patients with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes: a retrospective study显示文摘Background:Studies on the relationship between antiepileptic drug (AED) administration and clinical outcomes in patients with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) remain scarce. Levetiracetam (LEV) is an AED that is neuroprotective in various neurologic disorders. This study aimed to determine the impact of LEV on the outcome of MELAS.Methods:A retrospective, single-center study was performed based on a large cohort of patients with MELAS with a history of seizures (n=102). Decisions on antiepileptic therapies were made empirically. Patients were followed up for 1 to 8 years (median, 4 years) and divided into 2 groups based on whether LEV was administered (LEV or non-LEV). The modified Rankin scale (mRS) scores and mortality risks were analyzed in all patients.Results:LEV, carbamazepine, benzodiazepines, topiramate, oxcarbazepine, valproate, and lamotrigine were administered in 48, 37, 18, 13, 11, 9, and 9 patients, singly or in combination, respectively. The mean mRS score of the LEV group (n=48) was lower than that of the non-LEV group (n=54;mean±standard deviation, 2.79±1.47 vs. 3.83±1.93, P=0.006) up to the end of the study. Nevertheless, there was no difference in the proportion of subjects without disability (mRS ranging 0-1) between the groups (P=0.37). The multivariate regressions revealed that LEV treatment was associated with lower mRS scores (odds ratio 0.32, 95% confidence interval [CI] 0.15-0.68, P=0.003) and mortality rates (hazard ratio 0.24, 95% CI 0.08-0.74, P=0.013). There was a significant difference in the Kaplan-Meier survival curves between the groups (χ^2=4.29, P=0.04).Conclusions:The LEV administration is associated with lower mortality in patients with MELAS in this retrospective study. Further laboratory research and prospective cohort studies are needed to confirm whether LEV has neuroprotective effects on patients with mitochondrial diseases. | Zhe Zhang Dan-Hua Zhao Xu-Tong Zhao Xiao Zhang Hui Xiong Xin-Hua Bao Yun Yuan Zhao-Xia Wang | 2019 | Chinese Medical Journal2019，，3： | 4 |
| 19 | Low serum osteocalcin levels are correlated with left ventricular systolic dysfunction and cardiac death in Chinese men显示文摘Osteocalcin is a newly identified type of cytokine secreted by osteoblasts, which has an endocrine function, mediates energy and glycol-lipid metabolism, and is closely related to cardiovascular diseases. In this study, we investigated the value of serum osteocalcin levels in predicting left ventricular systolic dysfunction and cardiac death. A total of 258 patients in the Department of Cardiology were included. Two-dimensional echocardiography was performed in all the subjects. The cardiac death of subjects occurri ng with a media n follow-up of 4.6 years was in formed via phone calls or the electr onic medical records. The serum osteocalcin levels were measured using electrochemiluminescent immunoassay. We found that the median left ventricular ejection fractions (LVEFs) were 62% in men and 63% in women. In the men with a LVEF > 62%, the serum osteocalcin levels were significantly higher than in those with LVEF < 62%(P = 0.042), whereas this differenee was absent in the women. Both the serum osteocalcin (0 = 0.095, P = 0.028) and serum N-terminal pro-brain natriuretic peptide (NT-pro-BNP;p =—0.003, P<0.01) levels remained independently significantly correlated with LVEF in the men but not in the women. Receiver operating characteristic (ROC) analyses of the men revealed that the serum osteocalcin (P = 0.007), serum NT-pro-BNP (P = 0.018) and serum osteocalcin 4- NT-pro-BNP (P< 0.01) levels were all sign ificant in identify ng left ven tricular systolic dysf un ction at baseli ne, but the pairwise comparisons of the three areas under the curves (AUCs) were all non-significant. The men in the lower osteocalcin level group at baseline suffered a greater risk of future cardiac death than those in the higher osteocalcin level group, whereas the result for NT-pro-BNP exhibited the opposite pattern. In conclusion, Iower serum osteocalcin levels in the men could identify left ventricular systolic dysfunction and cardiac death in a manner that was not inferior to high serum NT-pro-BNP levels. | Xue-li Zhang Yun Shen Xiao-jing Ma Zhi-gang Lu Yi-ting Xu Qin Xiong Yu-qian Bao | 2019 | Acta Pharmacologica Sinica2019，40，4： | 4 |
| 20 | Strategies of Removing Residual Lithium Compounds on the Surface of Ni-Rich Cathode Materials显示文摘Ni-rich cathode materials have become one of the most promising cathode materials for advanced high-energy Li-ion batteries(LIBs)owing to their high specific capacity.However,Ni-rich cathode materials are sensitive to the trace H2O and CO2 in the air,and tend to react with them to generate LiOH and Li2COg at the particle surface region(named residual lithium compounds,labeled as RLCs).The RLCs will deteriorate the comprehensive performances of Ni-rich cathode materials and make trouble in the subsequent manufacturing process of electrode,including causing low initial coulombic efficiency and poor storage property,bringing about potential safety hazards,and gelatinizing the electrode slurry.Therefore,it is of considerable significance to remove the RLCs.Researchers have done a lot of work on the corresponding field,such as exploring the formation mechanism and elimination methods.This paper investigates the origin of the surface residual lithium compounds on Ni-rich cathode materials,analyzes their adverse effects on the per-formance and the subsequent electrode production process,and summarizes various kinds of feasible methods for removing the RLCS.Finally,we propose a new research direction of eliminating the lithium residuals after comparing and summing up the above.We hope this work can provide a reference for alleviating the adverse effects of residual lithium compounds for Ni-rich cathode materials'industrial production. | Yuefeng Su Linwei Li Gang Chen Lai Chen Ning Li Yun Lu Liying Bao Shi Chen Feng Wu | 2021 | Chinese Journal of Chemistry2021，39，1： | 4 |
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