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| 1 | Wnt/β-catenin signalling:function,biological mechanisms,and therapeutic opportunities显示文摘The Wnt/β-catenin pathway comprises a family of proteins that play critical roles in embryonic development and adult tissue homeostasis.The deregulation of Wnt/β-catenin signalling often leads to various serious diseases,including cancer and non-cancer diseases.Although many articles have reviewed Wnt/β-catenin from various aspects,a systematic review encompassing the origin,composition,function,and clinical trials of the Wnt/β-catenin signalling pathway in tumour and diseases is lacking.In this article,we comprehensively review the Wnt/β-catenin pathway from the above five aspects in combination with the latest research.Finally,we propose challenges and opportunities for the development of small-molecular compounds targeting the Wnt signalling pathway in disease treatment. | Jiaqi Liu Qing Xiao Jiani Xiao Chenxi Niu Yuanyuan Li Xiaojun Zhang Zhengwei Zhou Guang Shu Gang Yin | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 28 |
| 2 | Diffusion tensor imaging detects Wallerian degeneration of the corticospinal tract early after cerebral infarction显示文摘To investigate the feasibility and time window of early detection of Wallerian degeneration in the corticospinal tract after middle cerebral artery infarction,23 patients were assessed using magnetic resonance diffusion tensor imaging at 3.0T within 14 days after the infarction.The fractional anisotropy values of the affected corticospinal tract began to decrease at 3 days after onset and decreased in all cases at 7 days.The diffusion coefficient remained unchanged.Experimental findings indicate that diffusion tensor imaging can detect the changes associated with Wallerian degeneration of the corticospinal tract as early as 3 days after cerebral infarction. | Ruiman Xie Min Fang Linjiang Zhou Shanghua Fan Jianying Liu Hongbo Quan Man Luo Dongying Qiu | 2012 | Neural Regeneration Research2012，7，12： | 20 |
| 3 | A heat-driven thermoacoustic cryocooler capable of reaching below liquid hydrogen temperature显示文摘A 'double-gas acoustic amplifier' is introduced to couple a thermoacoustic heat engine and a two-stage pulse tube cooler in this paper. Compared with previous acoustic amplifiers, this new acoustic amplifier maintains the function of amplification for pressure amplitude. In particular, the novel acoustic amplifier with a reservoir makes it possible to install an acoustic transparent but gas blocking elastic membrane between the engine and the cooler. Thus, the engine can use nitrogen as the working gas to work at low frequency; and meanwhile, the cooler can still use helium as the working gas to maintain its high performance. With this new amplifier, the cooling temperature of a two-stage pulse tube cooler driven by an energy-focused thermoacoustic engine reached 18.7 K. | HU JianYing LUO ErCang DAI Wei Zhou Yuan | 2007 | Chinese Science Bulletin2007，52，4： | 18 |
| 4 | 大数据资源规划研究框架的构建显示文摘大数据资源规划是组织和国家管理大数据资源初期解决'如何开始,从哪里开始'的关键环节。基于对国内外大数据资源规划研究进展的系统梳理,本文从实践视角和理论视角对大数据资源规划研究进行了科学定位,并结合战略规划理论与信息资源规划理论,从宏观、中观和微观三个层面构建了大数据资源规划的研究框架。通过研究,得出如下结论:(1)大数据应用尚处在起步阶段,在此阶段研究大数据资源规划具有现实意义;(2)国内外学者已经着手研究大数据资源管理与规划,但研究视角有所差异,研究内容尚未系统化;(3)大数据资源、大数据资源管理需要从广义和狭义明晰地界定;(4)战略规划与信息资源规划理论都经历一个从微观到宏观的发展历程,为大数据资源规划研究提供了理论参考;(5)需要从国家、领域、组织三个视角构建大数据资源规划的研究框架,探索其研究的共性与个性问题。 | 周耀林 赵跃 Zhou Jiani | 2017 | 图书情报知识2017，34，4： | 12 |
| 5 | Hybrid hierarchy storage system in MilkyWay-2 supercomputer显示文摘 | Weixia XU Yutong LU Qiong LI Enqiang ZHOU Zhenlong SONG Yong DONG Wei ZHANG Dengping WEI Xiaoming ZHANG Haitao CHEN Jianying XING Yuan YUAN | 2014 | Frontiers of Computer Science2014，8，3： | 11 |
| 6 | 热处理前后非脱气交联聚乙烯的介电性能和机械性能分析(英文)显示文摘In order to investigate the influence of cross-linking byproducts on dielectric and mechanical properties of 110k V cross-linked polyethylene(XLPE)cable insulation,we selected the undegassed XLPE cable insulation which is heated at 70℃in laboratory for different duration in terms of heating time,to prepare XLPE samples with different mass loss ratio.We carried out Fourier transform infrared spectroscopy(FTIR)test,electric breakdown test at 50 Hz,pulsed electro-acoustic(PEA)measurement and tensile test for XLPE samples.In FTIR spectrogram,the characteristic absorption peaks were observed to appear at 1 680 cm-1 and 1 600 cm-1,which can be related to two kinds of cross-linking byproducts includingα-methylstyrene and acetophenone.The intensity of these absorption peaks decreases with the increase of mass loss ratio.It can be proposed that the mass loss of XLPE samples in the process of thermal treatment is caused by volatiliztion ofα-methylstyrene and acetophenone.Therefore,the change of mass loss ratio can reflect the change of content ofα-methylstyrene and acetophenone in the process of thermal treatment.The results of electric breakdown test showed that the reduction ofα-methylstyrene and acetophenone leads to the enhancement of electric breakdown strength at 50 Hz.PEA measurement results showed that heterocharge is produced and accumulated when cross-linking byproducts are present,whereas no heterocharge is found whenα-methylstyrene and acetophenone are absent.The formation of heterocharge is attributed to ionization of cross-linking byproducts under electric stress.Based on these results,it can be suggested that when AC electric field is applied on XLPE samples,the occurrence of ionization of cross-linking byproducts results in the increase of free carriers in XLPE insulation,leading to a lower electric breakdown strength.The results of tensile test showed that tensile strength is proportional to the mass loss ratio of XLPE samples,which suggests that cross-linking byproducts can reduce the tensile strength of XLPE insulation. | LI Huan LI Jianying WANG Qimeng ZHOU Yunjie LI Honglei | 2015 | 高电压技术2015，41，4： | 11 |
| 7 | Treatment of multiple sclerosis by transplantation of neural stem cells derived from induced pluripotent stem cells显示文摘Multiple sclerosis(MS) is an autoimmune disease of the central nervous system(CNS), with focal T lymphocytic infiltration and damage of myelin and axons. The underlying mechanism of pathogenesis remains unclear and there are currently no effective treatments. The development of neural stem cell(NSC) transplantation provides a promising strategy to treat neurodegenerative disease. However, the limited availability of NSCs prevents their application in neural disease therapy. In this study, we generated NSCs from induced pluripotent stem cells(iPSCs) and transplanted these cells into mice with experimental autoimmune encephalomyelitis(EAE), a model of MS. The results showed that transplantation of iPSC-derived NSCs dramatically reduced T cell infiltration and ameliorated white matter damage in the treated EAE mice. Correspondingly, the disease symptom score was greatly decreased, and motor ability was dramatically rescued in the iPSC-NSC-treated EAE mice, indicating the effectiveness of using iPSC-NSCs to treat MS. Our study provides pre-clinical evidence to support the feasibility of treating MS by transplantation of iPSC-derived NSCs. | Chao Zhang Jiani Cao Xiaoyan Li Haoyu Xu Weixu Wang Libin Wang Xiaoyang Zhao Wei Li Jianwei Jiao Baoyang Hu Qi Zhou Tongbiao Zhao | 2016 | Science China(Life Sciences)2016，59，9： | 9 |
| 8 | Sintilimab versus docetaxel as second-line treatment in advanced or metastatic squamous non-small-cell lung cancer:an open-label,randomized controlled phase 3 trial(ORIENT-3)显示文摘Background:Treatment options for Chinese patients with locally advanced or metastatic squamous-cell non-small-cell lung cancer(sqNSCLC)after failure of first-line chemotherapy are limited.This study(ORIENT-3)aimed to evaluate the efficacy and safety of sintilimab versus docetaxel as second-line treatment in patients with locally advanced or metastatic sqNSCLC.Methods:ORIENT-3 was an open-label,multicenter,randomized controlled phase 3 trial that recruited patients with stage IIIB/IIIC/IV sqNSCLC after failure with first-line platinum-based chemotherapy.Patients were randomized in a 1:1 ratio to receive either 200 mg of sintilimab or 75 mg/m^(2) of docetaxel intravenously every 3 weeks,stratified by the Eastern Cooperative Oncology Group performance status.The primary endpoint was overall survival(OS)in the full analysis set(FAS).Secondary endpoints included progression-free survival(PFS),objective response rate(ORR),disease control rate(DCR),duration of response(DoR)and safety.Results:Between August 25,2017,and November 7,2018,290 patients were randomized.For FAS,10 patients fromthe docetaxel armwere excluded.Themedian OS was 11.79(n=145;95%confidence interval[CI],10.28-15.57)months with sintilimab versus 8.25(n=135;95%CI,6.47-9.82)months with docetaxel(hazard ratio[HR]:0.74;95%CI,0.56-0.96;P=0.025).Sintilimab treatment significantly prolonged PFS(median 4.30 vs.2.79 months;HR:0.52;95%CI,0.39-0.68;P<0.001)and showed higher ORR(25.50%vs.2.20%,P<0.001)and DCR(65.50%vs.37.80%,P<0.001)than the docetaxel arm.The median DoRwas 12.45(95%CI,4.86-25.33)months in the sintilimab arm and 4.14(95%CI,1.41-7.23)months in the docetaxel arm(P=0.045).Treatment-related adverse events of grade≥3were reported in 26(18.1%)patients in the sintilimab arm and 47(36.2%)patients in the docetaxel arm.Exploratory biomarker analysis showed potential predictive values of expression levels of two transcription factors,including OVOL2(HR:0.35;P<0.001)and CTCF(HR:3.50;P<0.001),for sintilimab treatment.Conclusions:Compared with docetaxel,sintilimab significantly improved the OS,PFS,and ORR of Chinese patients with previously treated locally advanced or metastatic sqNSCLC. | Yuankai Shi Lin Wu Xinmin Yu Puyuan Xing Yan Wang Jianying Zhou Airong Wang Jianhua Shi Yi Hu Ziping Wang Guangyu An Yong Fang Sanyuan Sun Caicun Zhou Changli Wang Feng Ye Xingya Li Junye Wang Mengzhao Wang Yunpeng Liu Yanqiu Zhao Ying Yuan Jifeng Feng Zhendong Chen Jindong Shi Tao Sun Gang Wu Yongqian Shu Qisen Guo Yi Zhang Yong Song Shucai Zhang Yuan Chen Wei Li Hongrui Niu Wenwei Hu Lijun Wang Jianan Huang Yang Zhang Ying Cheng Zhengdong Wu Bo Peng Jiya Sun Christoph Mancao Yanqi Wang Luyao Sun | 2022 | Cancer Communications2022，42，12： | 8 |
| 9 | Simultaneous attack of a stationary target using multiple missiles: a consensus-based approach显示文摘This paper considers the simultaneous attack problem of multiple missiles against a stationary target. Built upon the classic proportional navigation structure, we propose a consensus-based approach to design the cooperative guidance law. Specifically, we present time-varying navigation ratios for the missiles,which exchange the time-to-go estimates between neighboring missiles via a communication network. For the cases where the communication topology is undirected or in the leader–follower structure with a missile acting as the leader whose navigation ratio cannot be tuned, we show that the proposed cooperative guidance law can solve the simultaneous attack problem. The effectiveness of the theoretical results is finally illustrated by numerical simulations. | Jialing ZHOU Jianying YANG Zhongkui LI | 2017 | Science China(Information Sciences)2017，60，7： | 8 |
| 10 | Toripalimab plus chemotherapy as second-line treatment in previously EGFR-TKI treated patients with EGFR-mutant-advanced NSCLC:a multicenter phase-II trial显示文摘This multicenter phase-II trial aimed to investigate the efficacy,safety,and predictive biomarkers of toripalimab plus chemotherapy as second-line treatment in patients with EGFR-mutant-advanced NSCLC.Patients who failed from first-line EGFR-TKIs and did not harbor T790M mutation were enrolled.Toripalimab plus carboplatin and pemetrexed were administrated every three weeks for up to six cycles,followed by the maintenance of toripalimab and pemetrexed.The primary endpoint was objective-response rate(ORR).Integrated biomarker analysis of PD-L1 expression,tumor mutational burden(TMB),CD8+tumor-infiltrating lymphocyte(TIL)density,whole-exome,and transcriptome sequencing on tumor biopsies were also conducted.Forty patients were enrolled with an overall ORR of 50.0%and disease-control rate(DCR)of 87.5%.The median progression free survival(PFS)and overall survival were 7.0 and 23.5 months,respectively.The most common treatment-related adverse effects were leukopenia,neutropenia,anemia,ALT/AST elevation,and nausea.Biomarker analysis showed that none of PD-L1 expression,TMB level,and CD8+TIL density could serve as a predictive biomarker.Integrated analysis of whole-exome and transcriptome sequencing data revealed that patients with DSPP mutation had a decreased M2 macrophage infiltration and associated with longer PFS than those of wild type.Toripalimab plus chemotherapy showed a promising anti-tumor activity with acceptable safety profiles as the second-line setting in patients with EGFR-mutant NSCLC.DSPP mutation might serve as a potential biomarker for this combination.A phase-III trial to compare toripalimab versus placebo in combination with chemotherapy in this setting is ongoing(NCT03924050). | Tao Jiang Pingyang Wang Jie Zhang Yanqiu Zhao Jianying Zhou Yun Fan Yongqian Shu Xiaoqing Liu Helong Zhang Jianxing He Guanghui Gao Xiaoqian Mu Zhang Bao Yanjun Xu Renhua Guo Hong Wang Lin Deng Ningqiang Ma Yalei Zhang Hui Feng Sheng Yao Jiarui Wu Luonan Chen Caicun Zhou Shengxiang Ren | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 8 |
| 11 | Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys显示文摘 | Yongchang Chen Yiqiang Cui Bin Shen Yuyu Niu Xiaoyang Zhao Lei Wang Jianying Wang Wei Li Qi Zhou Weizhi Ji Jiahao Sha Xingxu Huang | 2015 | Cell Research2015，25，2： | 8 |
| 12 | Cooperative Guidance Law Design for Simultaneous Attack with Multiple Missiles Against a Maneuvering Target显示文摘这份报纸对一个调遣的目标考虑多重导弹的同时的攻击问题。与大多数同时的攻击问题在被提出的存在文学不同当导弹 time-to-go 的一个一致问题估计,这份报纸作为导弹 ranges-to-go 的一致问题提出它。基于这策略,新奇分布式的指导法律被建议与未知可操作性的目标解决同时的攻击问题。适应控制方法被介绍估计目标加速的上面的界限。建议指导法律的有效性理论上并且数字地被验证。 | ZHOU Jialing Lü Yuezu LI Zhongkui YANG Jianying | 2018 | Journal of Systems Science & Complexity2018，31，1： | 8 |
| 13 | Lingguizhugan Decoction, a Chinese herbal formula, improves insulin resistance in overweight/obese subjects with non-alcoholic fatty liver disease: a translational approach显示文摘Lingguizhugan Decoction(LGZG)has been investigated in basic studies,with satisfactory effects on insulin resistance in non-alcoholic fatty liver disease(NAFLD).This translational approach aimed to explore the effect and underlying mechanism of LGZG in clinical setting.A randomized,double-blinded,placebo-controlled trial was performed.A total of 243 eligible participants with NAFLD were equally allocated to receive LGZG(two groups:standard dose and low dose)or placebo for 12 weeks on the basis of lifestyle modifications.The primary efficacy variable was homeostasis model assessment of insulin resistance(HOMA-IR).Analyses were performed in two populations in accordance with body mass index(BMI;overweight/obese,BMI 24 kg/m^(2);lean,BMI<24 kg/m^(2)).For overweight/obese participants,low-dose LGZG significantly decreased their HOMA-IR level compared with placebo(0.19(1.47)versus 0.08(1.99),P=0.038).For lean subjects,neither dose of LGZG showed a superior effect compared with placebo.Methylated DNA immunoprecipitation sequencing and real-time qPCR found that the DNA N6-methyladenine modification levels of protein phosphatase 1 regulatory subunit 3A(PPP1R3A)and autophagy related 3(ATG3)significantly increased after LGZG intervention in overweight/obese population.Low-dose LGZG effectively improved insulin resistance in overweight/obese subjects with NAFLD.The underlying mechanism may be related to the regulation of DNA N6-methyladenine modification of PPP1R3A and ATG3.Lean subjects may not be a targeted population for LGZG. | Liang Dai Jingjuan Xu Baocheng Liu Yanqi Dang Ruirui Wang Lijie Zhuang Dong Li Lulu Jiao Jianying Wang Lei Zhang Linda LDZhong Wenjun Zhou Guang Ji | 2022 | Frontiers of Medicine2022，16，5： | 7 |
| 14 | Transgenic breeding of anti-Bombyx mori L. nuclear polyhedrosis virus silkworm Bombyx mori显示文摘对 Bombyx 粗腐殖质 i L 抵抗的蚕紧张。原子 polyhedrosis 病毒通过转基因的实验被获得。与一个 A3 倡导者一起的小猪用绳子拖的平底渡船 transposon 随机被插入到蚕,驾驶进蚕染色体的提高的绿荧光灯的蛋白质(EGFP ) 记者基因。聚合酶链反应结果证实体外的 EGFP 基因的插入,和荧光显微镜学证明 EGFP 在中间的内脏织物被表示。原子 polyhedrosis 的病态比率在转基因的蚕紧张在原来的蚕紧张从 90% 减少了到 66.7% 。与到 Bombyx 粗腐殖质 i L 的抵抗相比。在 Qiufeng 紧张的原子 polyhedrosis 病毒,通常在生产被使用,有在转基因的蚕的 33 个百分的点的增加。在 ChunhuaxQiuyue 紧张的 antivirotic 特性,从一个不同转基因的家庭被引起,比在 QiufengxBaiyu,另一个生气品种在生产使用了高是大约 10 个百分的点。我们的结果在繁殖 anti-BmNPV 蚕紧张显示了插入 transposon 的变化的好申请价值。 | Huijuan Yang Wei Fan Hao Wei Jinwei Zhang Zhonghua Zhou Jianying Li Jianrong Lin Nong Ding Boxiong Zhong | 2008 | Acta Biochimica et Biophysica Sinica2008，40，10： | 6 |
| 15 | Growth and physiological responses of Agriophyllum squarrosum to sand burial stress显示文摘Agriophyllum squarrosum is an annual desert plant widely distributed on mobile and semi-mobile dunes in all the sandy deserts of China. We studied the growth and physiological properties of A. squarrosum seedlings under different sand burial depths in 2010 and 2011 at Horqin Sandy Land, Inner Mongolia to understand the ability and mechanism that A. squarrosum withstands sand burial. The results showed that A. squarrosum had a strong ability to withstand sand burial. Its survival rate, plant height and biomass increased significantly at a burial depth 25% of seedling height and decreased significantly only when the burial depth exceeded the height of the seedlings; some plants still survived even if the burial depth reached 266% of a seedling height. The malondialdehyde(MDA) content and membrane permeability of the plant did not change significantly as long as the burial depth was not greater than the seedling height; lipid peroxidation increased and cell membranes were damaged if the burial depth was increased further. When subjected to sand burial stress, superoxide dismutase(SOD) and peroxidase(POD) activities and free proline content increased in the seedlings, while the catalase(CAT) activity and soluble sugar content decreased. Sand burial did not lead to water stress. Reductions in photosynthetic area and cell membrane damage caused by sand burial may be the major mechanisms increasing mortality and inhibiting growth of the seedling. But the increases in SOD and POD activities and proline content must play a certain role in reducing sand burial damage. | Jin LI Hao QU HaLin ZHAO RuiLian ZHOU JianYing YUN ChengChen PAN | 2015 | Journal of Arid Land2015，7，1： | 5 |
| 16 | Theories,technologies and practices of lacustrine shale oil exploration and development:A case study of Paleogene Kongdian Formation in Cangdong sag,Bohai Bay Basin,China显示文摘As the main factors affecting stable and high production and the production regularity of lacustrine shale oil are unclear,the theoretical understandings,key exploration and development technologies,development effect and production regularity of lacustrine shale oil have been analyzed and summarized based on 700 m cores taken systematically from Paleogene Kong 2 Member of 4 wells in Cangdong sag,over 100000 analysis data and formation testing data.Three theoretical understandings on shale oil enrichment and high production have been reached:(1)High-quality shale with“three highs and one low”is the material base for shale oil enrichment.(2)Medium-slightly high thermal evolution degree is the favorable condition for shale oil enrichment.(3)Laminar felsic shale is the optimal shale layer for oil enrichment in semi-deep lake facies.Key exploration and development technologies such as shale oil enrichment layer and area evaluation and prediction,horizontal well pattern layout,shale oil reservoir fracturing,optimization of shale oil production regime have been established to support high and stable shale oil production.Under the guidance of these theoretical understandings and technologies,shale oil in Cangdong sag has achieved high and stable production,and 4 of them had the highest production of over 100 tons a day during formation testing.In particular,Well GY5-1-1 L had a daily oil production of 208 m^(3).By April,2022,the 28 wells combined have a stable oil production of 300–350 tons a day,and have produced 17.8×10^(4) t of oil cumulatively.It is found that the shale oil production of horizontal well declines exponentially in natural flow stage,and declines in step pattern and then tends stable in the artificial lift stage.Proportion of light hydrocarbons in produced shale oil is in positive correlation with daily oil production and decreases regularly during production test. | ZHAO Xianzheng ZHOU Lihong PU Xiugang JIN Fengming HAN Wenzhong SHI Zhannan CHEN Changwei JIANG Wenya GUAN Quansheng XU Jing LIU Xuewei ZHANG Wei MA Jianying | 2022 | Petroleum Exploration and Development2022，49，3： | 4 |
| 17 | A Simulation Engine for Stochastic Timed Petri Nets and Application to Emergency Healthcare Systems显示文摘In many service delivery systems,the quantity of available resources is often a decisive factor of service quality.Resources can be personnel,offices,devices,supplies,and so on,depending on the nature of the services a system provides.Although service computing has been an active research topic for decades,general approaches that assess the impact of resource provisioning on service quality matrices in a rigorous way remain to be seen.Petri nets have been a popular formalism for modeling systems exhibiting behaviors of competition and concurrency for almost a half century.Stochastic timed Petri nets(STPN),an extension to regular Petri nets,are a powerful tool for system performance evaluation.However,we did not find any single existing STPN software tool that supports all timed transition firing policies and server types,not to mention resource provisioning and requirement analysis.This paper presents a generic and resource oriented STPN simulation engine that provides all critical features necessary for the analysis of service delivery system quality vs.resource provisioning.The power of the simulation system is illustrated by an application to emergency health care systems. | Jiani Zhou Jiacun Wang Jun Wang | 2019 | IEEE/CAA Journal of Automatica Sinica2019，6，4： | 4 |
| 18 | Inhibition of botulinum toxin's association with rat brain synaptosomes by toosendanin,an antibotulismic triterpenoid显示文摘BACKGROUND: Toosendanin (TSN), a triterpenoid derivative extracted from the bark of Melia toosendanin Sieb et Zucc, has been demonstrated to be an effective drug for treatment of experimental botulism. OBJECTIVE: To explore its antibotulismic mechanism by observing the effect of toosendanin on association of botulinum toxin (BoTx) with rat brain synaptosomes under different conditions. DESIGN: A randomized controlled experiment. SETTING: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. MATERIALS: Sprague-Dawley rats, weighing (220±12) g, were involved. TSN was a sample recrystallized in ethanol with a purity > 98%. BoTx/A and BoTx/C of 500 ku (1.8×107 mouse LD50 /mg) were purchased from Wako (Japan), Horse antitoxins to BoTx/A and /C (primary antibodies) were purchased from Lanzhou Institute of Biological Products (China). METHODS: Major experiments were finished between March 2005 and October 2005 in key laboratory of neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. ① Preparation of synaptosome of rats: one aliquot of synaptosome was pre-incubated with TSN (17 μmol/L) for 20 minutes at 4 ℃ or 37 ℃ (another aliquot of synaptosome which was untouched was used as control). Then, BoTx was respectively added for another 20-minute incubation. ② A homochronous experiment was still performed at 37 ℃. Differently, high level of K+ was used to stimulate synaptosome for 25 minutes. Two aliquots of synaptosomes with or without TSN (17 μmol/L)were preincubated for 15 minutes. Then, 30 mmol/L KCl was separately added in two aliquots, 5 minutes later, 13 nmol/L BoTx/C was separately added followed by 20-minute incubation. ③The effect of TSN on BoTx binding was observed by Western blot and synchronization method. ④ t test was used for comparing the difference of measurement data. MAIN OUTCOME MEASURES: The gray value of BoTx bands from western blot was used to estimate the bound amount of BoTx. RESULTS: ① Preincubation of synaptosomes with TSN (17 μmol/L) at 4 ℃ decreased the association of BoTx type A (BoTx/A) by (11.4±8.7) % (P < 0.05, n =8) and that of BoTx type C (BoTx/C) by (9.2±2.8)% (P < 0.05, n =8). ② When incubation temperature was set at 37 ℃, preincubation of synaptosomes with TSN (17 μmol/L) decreased the association of BoTx/C by (18.0 ± 6.7)% (n =5, vs. the control without TSN at 37 ℃, P < 0.05), but there was no significant change observed when decreasing TSN to a concentration of 0.17 μmol/L. ③ When synaptosomes preincubated with TSN in high level of K+ at 37 ℃, the inhibition of TSN on BoTx/C association further increased to (32.6±6.1)% (n =4, vs the control without TSN at 37 ℃ with high level of K+, P < 0.05). CONCLUSION: These results indicate that TSN inhibits the binding step of BoTx intoxication, and probably its subsequent temperature- and depolarization- dependent internalization of toxin. | Jianying Zhou Yuliang Shi | 2006 | Neural Regeneration Research2006，1，6： | 3 |
| 19 | Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos显示文摘 | Yuyu Niu Bin Shen Yiqiang Cui Yongchang Chen Jianying Wang Lei Wang Yu Kang Xiaoyang Zhao Wei Si Wei Li Andy Peng Xiang Jiankui Zhou Xuejiang Guo Ye Bi Chenyang Si Bian Hu Guoying Dong Hong Wang Zuomin Zhou Tianqing Li Tao Tan Xiuqiong Pu Fang Wang Shaohu | 2014 | Cell2014，，： | 3 |
| 20 | A novel glasses-free virtual reality rehabilitation system on improving upper limb motor function among patients with stroke:A feasibility pilot study显示文摘Background:Virtual reality(VR)technology is increasingly used in stroke rehabilitation.This study aimed to investigate the effectiveness of using the glasses-free VR training to improve motor function of upper limb in patients with stroke.Methods:Twelve patients with stroke were recruited to participate in the intervention of 3 weeks.At the baseline and post intervention,two times of evaluation including Fugl-Meyer upper-extremity scale(FMS-UE),transcranial magnetic stimulation(TMS)measurement and motion evaluation were performed.Results:No significant difference was observed between two groups at baseline evaluation.After the intervention,the FMS-UE scores presented a greater improvement in the VR group compared with the control group.TMS measurement showed that there was significant difference in cortex latency and central motor conduction time between two groups after the intervention,but no significant difference in the amplitude of motor event potential was observed.In addition,there was a significant correlation between game scores and FMS-UE scores.Conclusions:The novel glasses-free VR training was at least as effective as conventional occupational therapy in upper limb motor function,improving nerve conduction time and corticospinal excitability in patient with stroke. | Haoyu Xie Hantao Zhang Haowen Liang Hang Fan Jianying Zhou Wai Leung Ambrose Lo Le Li | 2021 | Medicine in Novel Technology and Devices2021，，3： | 2 |
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