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| 1 | Survival of transplanted neurotrophin-3 expressing human neural stem cells and motor function in a rat model of spinal cord injury显示文摘<正>BACKGROUND:Many methods have been attempted to repair nerves following spinal cord injury, including peripheral nerve transplantation,Schwann cell transplantation,olfactory ensheathing cell transplantation,and embryonic neural tissue transplantation.However,there is a need for improved outcomes. OBJECTIVE:To investigate the repair feasibility for rat spinal cord injury using human neural stem cells(hNSCs) genetically modified by lentivirus to express neurotrophin-3. DESIGN,TIME AND SETTING:In vitro cell biological experiment and in vivo randomized,controlled, genetic engineering experiment were performed at the Third Military Medical University of Chinese PLA and First People's Hospital of Yibin,China from March 2006 to December 2007. MATERIALS:A total of 64 adult,female,Wistar rats were used for the in vivo study.Of them,48 rats were used to establish models of spinal cord hemisection,and were subsequently equally and randomly assigned to model,genetically modified hNSC,and normal hNSC groups.The remaining 16 rats served as normal controls. METHODS:hNSCs were in vitro genetically modified by lentivirus to secrete both green fluorescence protein and neurotrophin-3.Neurotrophin-3 expression was measured by Western blot. Genetically modified hNSC or normal hNSC suspension(5×10~5) was injected into the rat spinal cord following T_(10) spinal cord hemisection.A total of 5μL Dulbecco's-modified Eagle's medium was infused into the rat spinal cord in the model group.Transgene expression and survival of transplanted hNSCs were determined by immunohistochemistry.Motor function was evaluated using the Basso,Beattie,and Bresnahan(BBB) scale. MAIN OUTCOME MEASURES:The following parameters were measured:expression of neurotrophin-3 produced by genetically modified hNSCs,transgene expression and survival of hNSCs in rats,motor function in rats. RESULTS:hNSCs were successfully genetically modified by lentivirus to stably express neurotrophin-3.The transplanted hNSCs primarily gathered at,or around,the injection site two weeks following transplantation,and gradually migrated towards the surrounding tissue. Transplanted hNSCs were observed 7.0-8.0 mm away from the injection site.In addition,hNSCs were observed 10 weeks after transplantation.At week 4,BBB locomotor scores were significantly greater in the genetically modified hNSC and normal hNSC groups,compared with the model group (P<0.05),and scores were significantly greater in the genetically modified hNSC group compared with the normal hNSC group(P<0.05). CONCLUSION:hNSCs were genetically modified with lentivirus to stably secrete neurotrophin-3. hNSCs improved motor function recovery in rats following spinal cord injury. | Peiqiang Cai Guangyun Sun Peishu Cai Martin Oudega Rui Xiao Xuewen Wang Wei Li Yunbing Shu Cheng Cai Haihao Yang Xuebing Shan Wuhua Luo | 2009 | Neural Regeneration Research2009，4，7： | 18 |
| 2 | A novel mechanism of inhibiting in-stent restenosis with arsenic trioxide drug-eluting stent: Enhancing contractile phenotype of vascular smooth muscle cells via YAP pathway显示文摘Objective:Arsenic trioxide(ATO or As2O3)has beneficial effects on suppressing neointimal hyperplasia and restenosis,but the mechanism is still unclear.The goal of this study is to further understand the mechanism of ATO's inhibitory effect on vascular smooth muscle cells(VSMCs).Methods and results:Through in vitro cell culture and in vivo stent implanting into the carotid arteries of rabbit,a synthetic-to-contractile phenotypic transition was induced and the proliferation of VSMCs was inhibited by ATO.F-actin filaments were clustered and the elasticity modulus was increased within the phenotypic modulation of VSMCs induced by ATO in vitro.Meanwhile,Yes-associated protein(YAP)nuclear translocation was inhibited by ATO both in vivo and in vitro.It was found that ROCK inhibitor or YAP inactivator could partially mask the phenotype modulation of ATO on VSMCs.Conclusions:The interaction of YAP with the ROCK pathway through ATO seems to mediate the contractile phenotype of VSMCs.This provides an indication of the clinical therapeutic mechanism for the beneficial bioactive effect of ATO-drug eluting stent(AES)on in-stent restenosis(ISR). | Yinping Zhao Guangchao Zang Tieying Yin Xiaoyi Ma Lifeng Zhou Lingjuan Wu Richard Daniel Yunbing Wang Juhui Qiu Guixue Wang | 2021 | Bioactive Materials2021，6，2： | 13 |
| 3 | Bone physiological microenvironment and healing mechanism:Basis for future bone-tissue engineering scaffolds显示文摘Bone-tissue defects affect millions of people worldwide.Despite being common treatment approaches,autologous and allogeneic bone grafting have not achieved the ideal therapeutic effect.This has prompted researchers to explore novel bone-regeneration methods.In recent decades,the development of bone tissue engineering(BTE)scaffolds has been leading the forefront of this field.As researchers have provided deep insights into bone physiology and the bone-healing mechanism,various biomimicking and bioinspired BTE scaffolds have been reported.Now it is necessary to review the progress of natural bone physiology and bone healing mechanism,which will provide more valuable enlightenments for researchers in this field.This work details the physiological microenvironment of the natural bone tissue,bone-healing process,and various biomolecules involved therein.Next,according to the bone physiological microenvironment and the delivery of bioactive factors based on the bone-healing mechanism,it elaborates the biomimetic design of a scaffold,highlighting the designing of BTE scaffolds according to bone biology and providing the rationale for designing next-generation BTE scaffolds that conform to natural bone healing and regeneration. | Guanyin Zhu Tianxu Zhang Miao Chen Ke Yao Xinqi Huang Bo Zhang Yazhen Li Jun Liu Yunbing Wang Zhihe Zhao | 2021 | Bioactive Materials2021，6，11： | 11 |
| 4 | Vascular restoration therapy and bioresorbable vascular scaffold显示文摘This article describes the evolution of minimally invasive intervention technologies for vascular restoration therapy from early-stage balloon angioplasty in 1970s,metallic bare metal stent and metallic drug-eluting stent technologies in 1990s and 2000s,to bioresorbable vascular scaffold(BVS)technology in large-scale development in recent years.The history,the current stage,the challenges and the future of BVS development are discussed in detail as the best available approach for vascular restoration therapy.The criteria of materials selection,design and processing principles of BVS,and the corresponding clinical trial results are also summarized in this article. | Yunbing Wang Xingdong Zhang | 2014 | Regenerative Biomaterials2014，1，1： | 10 |
| 5 | 17β-estradiol attenuates homocysteine-induced oxidative stress and inflammatory response as well as MAPKs cascade via activating PI3-K/Akt signal transduction pathway in Raw 264,7 cells显示文摘氧化应力,煽动性的反应,和激活 mitogen 的蛋白质 kinases (MAPK ) 串联是骨质疏松症的重要病原的因素。提高的 homocysteine (Hcy ) 可以在绝经后的骨质疏松症激活氧化应力和还原剂骨头矿物质密度,这被报导了。而且,荷尔蒙代替治疗广泛地在诊所被使用了与骨质疏松症阻止并且对待绝经后的女人, osteoporotic 骨折和位于 Hcy 的行动下面的相关信号 transduction 小径仍然保持不清楚。在这研究,我们调查了 17-estradiol 的效果(导致 Hcy 的氧化压力,煽动性的反应和 MAPK 串联,以及内在的信号 transduction 小径在上的 17-E 2) 鼠科未加工 264.7 个房间。反应的氧种类(ROS ) 被 fluorospectrophotometry 估计。proinflammatory cytokines 肿瘤坏死因素 --(TNF-) 并且 interleukin (IL )-1 被 double-immunofluorescence 标记和反向的 transcriptase 聚合酶链反应试金分析分别地。而且, MAPK 串联的 phosphorylation 层次被西方的污点分析测量。一个特定的 phosphatidylinositol 3-kinase (PI3-K ) 禁止者, Wortmannin (1 M ) 被采用决定 PI3-K/Akt 发信号小径是否调停了 17-E 2 的效果在上未加工 264.7 个房间。17-E 2 显著地减少了 ROS 生产由 Hcy 导致了,在蛋白质和 mRNA 的 TNF-1 和 IL-1 的表示铺平,并且下面调整 MAPK (ERK1/2, JNK 和 p38 ) 的 phosphorylation。导致 Hcy 的变化上的 17-E 2 的这些压制的效果被预告的处理与 PI3-K 禁止者 Wortmannin 颠倒。结果显示那 17-estradiol 可以稀释 MAPK 的导致 Hcy 的氧化应力,煽动性的反应和起来规定在未加工经由 PI3-K/Akt 的 264.7 个房间表明 transduction 小径。 | Ying Zhang Ying He Yi Zong Jiazhi Guo Lin Sun Yunbing Ma Wei Dong Li Gui | 2015 | Acta Biochimica et Biophysica Sinica2015，47，2： | 9 |
| 6 | Platelet Membrane-Coated Nanocarriers Targeting Plaques to Deliver Anti-CD47 Antibody for Atherosclerotic Therapy显示文摘Atherosclerosis,the principle cause of cardiovascular disease(CVD)worldwide,is mainly characterized by the pathological accumulation of diseased vascular cells and apoptotic cellular debris.Atherogenesis is associated with the upregulation of CD47,a key antiphagocytic molecule that is known to render malignant cells resistant to programmed cell removal,or“efferocytosis.”Here,we have developed platelet membrane-coated mesoporous silicon nanoparticles(PMSN)as a drug delivery system to target atherosclerotic plaques with the delivery of an anti-CD47 antibody.Briefly,the cell membrane coat prolonged the circulation of the particles by evading the immune recognition and provided an afinity to plaques and atherosclerotic sites.The anti-CD47 antibody then normalized the clearance of diseased vascular tissue and further ameliorated atherosclerosis by blocking CD47.In an atherosclerosis model established in ApoE^(-/-)mice,PMSN encapsulating anti-CD47 antibody delivery significantly promoted the efferocytosis of necrotic cells in plaques.Clearing the necrotic cells greatly reduced the atherosclerotic plaque area and stabilized the plaques reducing the risk of plaque rupture and advanced thrombosis.Overall,this study demonstrated the therapeutic advantages of PMSN encapsulating anti-CD47 antibodies for atherosclerosis therapy,which holds considerable promise as a new targeted drug delivery platform for efficient therapy of atherosclerosis。 | Liang Chen Zhongyi Zhou Cheng Hu Manfred F.Maitz Li Yang Rifang Luo Yunbing Wang | 2022 | Research2022，，3： | 5 |
| 7 | Prolonged electrical stimulation causes no damage to sacral nerve roots in rabbits显示文摘Previous studies have shown that, anode block electrical stimulation of the sacral nerve root can produce physiological urination and reconstruct urinary bladder function in rabbits. However, whether long-term anode block electrical stimulation causes damage to the sacral nerve root remains unclear, and needs further investigation. In this study, a complete spinal cord injury model was established in New Zealand white rabbits through T9–10 segment transection. Rabbits were given continuous electrical stimulation for a short period and then chronic stimulation for a longer period. Results showed that compared with normal rabbits, the structure of nerve cells in the anterior sacral nerve roots was unchanged in spinal cord injury rabbits after electrical stimulation. There was no significant difference in the expression of apoptosis-related proteins such as Bax, Caspase-3, and Bcl-2. Experimental findings indicate that neurons in the rabbit sacral nerve roots tolerate electrical stimulation, even after long-term anode block electrical stimulation. | Peng Yan Xiaohong Yang Xiaoyu Yang Weidong Zheng Yunbing Tan | 2014 | Neural Regeneration Research2014，9，12： | 3 |
| 8 | On-line near-infrared spectroscopy optimizing and monitoring biotransformation process of γ-aminobutyric acid显示文摘Near-infrared spectroscopy(NIRS) with its fast and nondestructive advantages can be qualified for the real-time quantitative analysis.This paper demonstrates that NIRS combined with partial least squares(PLS) regression can be used as a rapid analytical method to simultaneously quantify L-glutamic acid(LGlu) and γ-aminobutyric acid(GABA) in a biotransformation process and to guide the optimization of production conditions when the merits of NIRS are combined with response surface methodology.The high performance liquid chromatography(HPLC) reference analysis was performed by the o-phthaldialdehyde pre-column derivatization.NIRS measurements of two batches of 141 samples were firstly analyzed by PLS with several spectral pre-processing methods.Compared with those of the HPLC reference analysis,the resulting determination coefficients(R^2),root mean square error of prediction(RMSEP) and residual predictive deviation(RPD) of the external validation for the L-Glu concentration were 99.5%,1.62 g/L,and11.3,respectively.For the GABA concentration,R^2,RMSEP,and RPD were 99.8%,4.00 g/L,and 16.4,respectively.This NIRS model was then used to optimize the biotransformation process through a BoxBehnken experimental design.Under the optimal conditions without p H adjustment,200 g/L L-Glu could be catalyzed by 7148 U/L glutamate decarboxylase(GAD) to GABA,reaching 99% conversion at the fifth hour.NIRS analysis provided timely information on the conversion from L-Glu to GABA.The results suggest that the NIRS model can not only be used for the routine profiling of enzymatic conversion,providing a simple and effective method of monitoring the biotransformation process of GABA,but also be considered to be an optimal tool to guide the optimization of production conditions. | Guoyu Ding Yuanyuan Hou Jiamin Peng Yunbing Shen Min Jiang Gang Bai | 2016 | Journal of Pharmaceutical Analysis2016，6，3： | 2 |
| 9 | Two-photon AIE probe conjugated theranostic nanoparticles for tumor bioimaging and pH-sensitive drug delivery显示文摘Nanoparticles armed with chemotherapy drug and fluorescence probe have become an effective anticancer strategy for their advantages in cancer diagnosis and treatment.However,fluorophore for diagnostic medicine with deep penetration depth and high resolution are still very rare,while rational designs are also required to improve the tumor retention and target-site drug delivery.Herein,a two-photon fluorophore with aggregation-induced emission and large two-photon absorption cross-section has been designed for two-photon bioimaging,and a novel theranostic nanoplatform is also constructed based on doxorubicin and the two-photon fluorophore conjugated copolymer,P(TPMA-co-AEMA)-PEI(DA)-Blink-PEG (PAEEBlink-DA).The micelles maintain a “stealth” property during blood circulation and is activated in the acidic tumor microenvironment,which triggers the charge-conversion and results in enhanced micellar internalization.Meanwhile,PAEMA chains can convert from hydrophobicity to hydrophilicity with accelerated drug release and particle size expansion.The enlarged particle size would potentially extend the retention time of these micelles.Moreover,a great AIE active two-photon bioimaging with tissue penetration depth up to 150 μm is observed and the in vivo biodistribution of nanoparticles can be traced.The in vivo antitumor results further indicate the obvious reduction of adverse effect and enhanced treatment effect of these micelles,proving that these PAEEBlink-DA micelles would be a potential candidate for tumor theranostic applications. | Boxuan Ma Weihua Zhuang Haiyang He Xin Su Tao Yu Jun Hu Li Yang Gaocan Li Yunbing Wang | 2019 | Nano Research2019，12，7： | 2 |
| 10 | Electrospun silk fibroin/poly(L-lactide-ecaplacton)graft with platelet-rich growth factor for inducing smooth muscle cell growth and infiltration显示文摘The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering.However,smooth muscle cells(SMCs)penetration into the electrospun graft to form a smooth muscle layer is limited due to the dense packing of fibers and lack of inducing factors.In this paper,silk fibroin/poly(L-lactide-e-caplacton)(SF/PLLA-CL)vascular graft loaded with platelet-rich growth factor(PRGF)was fabricated by electrospinning.The in vitro results showed that SMCs cultured in the graft grew fast,and the incorporation of PRGF could induce deeper SMCs infiltrating compared to the SF/PLLA-CL graft alone.Mechanical properties measurement showed that PRGF-incorporated graft had proper tensile stress,suture retention strength,burst pressure and compliance which could match the demand of native blood vessel.The success in the fabrication of PRGF-incorporated SF/PLLA-CL graft to induce fast SMCs growth and their strong penetration into graft has important application for tissue-engineered blood vessels. | Anlin Yin Gary L.Bowlin Rifang Luo Xingdong Zhang Yunbing Wang Xiumei Mo | 2016 | Regenerative Biomaterials2016，3，4： | 2 |
| 11 | Posterior screw place- ment on the lateral mass of atlas: an anatomic study 显示文摘 | Hong X Dong Y Yunbing C | 2004 | Spine2004，29，5： | 1 |
| 12 | Integrated prodrug micelles with two-photon bioimaging and pH-triggered drug delivery for cancer theranostics显示文摘Nanodrug carriers with fluorescence radiation are widely used in cancer diagnosis and therapy due to their real-time imaging,less side effect,better drug utilization as well as the good bioimaging ability.However,traditional nanocarriers still suffer from unexpectable drug leakage,unsatisfactory tumor-targeted drug delivery and shallow imaging depth,which limit their further application in cancer theranostics.In this study,an integrated nanoplatform is constructed by polymeric prodrug micelles with two-photon and aggregation-induced emission bioimaging,charge reversal and drug delivery triggered by acidic pH.The prodrug micelles can be self-assembled by the TPPEI(DA/DOX)-PEG prodrug polymer,which consists of the two-photon fluorophore(TP),dimethylmaleic anhydride(DA)grafted polyethyleneimine(PEI)and polyethylene glycol(PEG).The PEG segment,DOX and DA are bridged to polymer by acid cleavable bonds,which provides the micelles a‘stealth’property and a satisfactory stability during blood circulation,while the outside PEG segment is abandoned along with the DA protection in the tumor acidic microenvironment,thus leading to charge reversal-mediated accelerated endocytosis and tumor-targeted drug delivery.The great antitumor efficacy and reduced side effect of these pH-sensitive prodrug micelles are confirmed by antitumor assays in vitro and in vivo.Meanwhile,these micelles exhibited great deep-tissue two-photon bioimaging ability up to 150 lm in depth.The great antitumor efficacy,reduced side effect and deep two-photon tissue imaging make the TP-PEI(DA/DOX)-PEG prodrug micelles would be an efficient strategy for theranostic nanoplatform in cancer treatment. | Hong Xu Boxuan Ma Jizhou Jiang Sutong Xiao Rongrong Peng Weihua Zhuang Gaocan Li Yunbing Wang | 2020 | Regenerative Biomaterials2020，7，2： | 1 |
| 13 | Charging for the waste dumping of open-pit metal mines显示文摘Based on the externality theory and the environmental value theory, the hypothesis of charging for waste dumping of open-pit metal mines was put forth. The charging methods were designed according to the characteristics of waste dumping of openpit metal mines, including charging based on the dumping amount of the total waste, multi-charging factors, exceeding standard punishment charging, and so on. The main charging parameter is based on the dumping area rather than the total amount of waste dumping.The charging model of waste dumping of open-pit mines was formulated, and the charging rate was divided into two parts, i.e., the standard charging rate and the differential charging rate. The standard charging rate was derived using the equilibrium dynamic model,whereas the differential one was obtained by establishing the fuzzy synthesized evaluation model. | Yunbing Hou Zhaoxiang Zhang Haifeng Duan Liming Xue | 2006 | Journal of University of Science and Technology Beijing2006，13，6： | 1 |
| 14 | Sensor and actuator fault isolation by structuredpartial PCA with nonlinear extensions显示文摘 | Huang Yunbing Gertler J McAvoy T J | 2000 | Journal of Process Control2000，10，5： | 1 |
| 15 | The new coal chemical industry technology progress显示文摘 | Huang Yunbing | | 0，，258： | 1 |
| 16 | Charging for the waste dumping of open-pit metal mines显示文摘 | Hou yunbing Zhang Zhaoxiang Duang I-taifeng | 2006 | Journal of University of Science and Technology Beijing Mineral Metallurgy Material2006，2006，6： | 1 |
| 17 | Catalytic oxidation of the dye wastewater with hydrogen peroxide显示文摘 | Qiu Zumin He Yunbing Liu Xiaocheng | 2005 | Chemical Engineering and Processing2005，44，： | 1 |
| 18 | Immunoproteomics analysis of whole cell bacterial proteins of Riemerella anatipestifer显示文摘 | Qinghai Hu Chan Ding Jing Tu Xiaolan Wang Xiangan Han Yunbing Duan Shengqing Yu | 2012 | Veterinary Microbiology (-)2012，，3： | 1 |
| 19 | Effective Capacity in Cognitive Radio Networks with Relay and Primary User Emulator显示文摘In this paper,the transmission performances are studied in cognitive radio networks with primary user emulator and relay existence.In the proposed network,the users include primary users,secondary users and primary user emulators.The decreasing access priority of the users are primary users,primary user emulators and secondary users.Different user access to the network results in different transmission effects.We impose interference power constraints on the secondary users to protect the primary users from being interfered.We also adopt the transmission mechanism that transits among more than one secondary transmitters,secondary receivers and relays.The transition models of the transmission states are proposed to describe the transmission mechanism.To investigate the transmission performances,the theory of effective capacity is adopted.The transmission performances in terms of effective capacity are expressed and demonstrated under different transmission policies.The overall effective capacity,as the overall data traffic in the cognitive radio network,is calculated.Besides,the overall effective capacity is demonstrated under different transmission strategies.The results show the greedy transmission strategy outperforms the rest of the transmission 8 policies in the overall effective capacity.For a larger number of the users,the effective capacity converges to a certain value. | Luyong Zhang Yijie Yang Xuan Li Jinhua Chen Yunbing Chi | 2019 | China Communications2019，16，11： | 1 |
| 20 | Posterior screw placement on the lateral mass of atlas: an anatomic study显示文摘 | Hong X Dong Y Yunbing C | 2004 | Spine (Phila Pa 1976)2004，29，： | 1 |
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