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| 1 | Microbiota transplantation: concept, methodology and strategy for its modernization显示文摘烘便的 microbiota 移植(FMT ) 在最近的年里成为了 biomedicine 和临床的药的一个研究焦点。从为不同疾病的 FMT 的临床的反应为与各种各样的混乱联系的 microbiota 主人相互作用提供了证据,包括 Clostridium 顽固感染,煽动性的肠疾病,糖尿病 mellitus,癌症,肝肝硬化, gutbrain 疾病和其它。为了讨论使用微生物从古老的中国治疗人的疾病到电流的经验,时代应该在向前移动标准化 FMT 并且完成更好的未来是重要的。这里,我们基于文学和州的专家观点从 FMT 考察 microbiota 移植的变化概念到选择 microbiota 移植, FMT 的方法论发展和 stepup FMT 策略。 | Faming Zhang Bota Cui Xingxiang He Yuqiang Nie Kaichun Wu Daiming Fan | 2018 | Protein & Cell2018，9，5： | 79 |
| 2 | Nondestructive 3D Image Analysis Pipeline to Extract Rice Grain Traits Using X-Ray Computed Tomography显示文摘The traits of rice panicles play important roles in yield assessment,variety classification,rice breeding,and cultivation management.Most traditional grain phenotyping methods require threshing and thus are time-consuming and labor-intensive;moreover,these methods cannot obtain 3D grain traits.In this work,based on X-ray computed tomography,we proposed an image analysis method to extract twenty-two 3D grain traits.After 104 samples were tested,the R^(2) values between the extracted and manual measurements of the grain number and grain length were 0.980 and 0.960,respectively.We also found a high correlation between the total grain volume and weight.In addition,the extracted 3D grain traits were used to classify the rice varieties,and the support vector machine classifier had a higher recognition accuracy than the stepwise discriminant analysis and random forest classifiers.In conclusion,we developed a 3D image analysis pipeline to extract rice grain traits using X-ray computed tomography that can provide more 3D grain information and could benefit future research on rice functional genomics and rice breeding. | Weijuan Hu Can Zhang Yuqiang Jiang Chenglong Huang Qian Liu Lizhong Xiong Wanneng Yang Fan Chen | 2020 | Plant Phenomics2020，2，1： | 6 |
| 3 | Recent Advances in Super-Resolution Fluorescence Imaging and Its Applications in Biology显示文摘Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes:(1) patterned illuminationbased super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures;(2) single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluorophore at subdiffraction precision;(3) bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected. | Rongcheng Han Zhenghong Li Yanyan Fan Yuqiang Jiang | 2013 | Journal of Genetics and Genomics2013，40，12： | 3 |
| 4 | Construction of New Genetically Engineered Vaccine Strain for O-type Foot-and-Mouth Disease of Pig显示文摘Foot-and-mouth disease is an acute and highly contagious viral infectious disease. Although the foot and mouth disease vaccine has been applied in some parts of the world since the beginning of the 20th century, the current epidemic of foot and mouth disease in the world is still serious and constitutes an obstacle to the trade of animal and animal products in the world. The porcine pseudorabies virus gene deletion strain PRV TK-/ gE-/ LacZ +, which is constructed by our laboratory, has the advantages of good safety, large capacity and high recombination effi ciency. In this study, the artifi cial O-type foot-and-mouth disease P1 gene as an antigen gene, UbiP1 fused with ubiquitin (Ub) as another antigen gene to enhance cellular immunity, and then shRNA designed for FMD 3B gene and porcine IFN-[gamma] with antiviral and immune-regulatory eff ects. The two functional genes were constructed in turn to construct a transfer vector with four functional genes. The transfer vector was transfected into TK-/ gE-/ LacZ+ cells with PRV deletion vector, and the plasmids were purifi ed and identifi ed. The recombinant pseudorabies virus, which contains the six functional genes of P1 gene, UbiP1, shRNA and IFN-γ, was obtained, which laid the foundation for further construction of new genetically engineered vaccine. | Feifei Liu Yuqiang Fan Xiaolin Lin | 2017 | Gene Science & Engineering2017，1，1： | 0 |
| 5 | Numerical Analysis of Residual Strength in the Perforated Casing of Ultra Deep Wells显示文摘A three-dimensional model for the numerical simulation of casing-cement behavior is used to investigate residual strength in the perforated casing of ultra deep wells.The influence of the hole diameter,hole density and phase angle on the residual strength of the casing under non-uniform stress and fracturing conditions is revealed through the consideration of different perforation parameters.It is shown that the residual strength of the casing increases with the hole diameter and periodically changes with the hole density;the phase angle is the main factor that affects the residual strength of the perforated casing,and the perforation should be avoided in the direction of the minimum principal stress to reduce stress concentration at the perforation hole.Moreover,as shown by a companion orthogonal experiment,the descending order of influence of the different influential parameters is:phase angle,hole diameter,hole density and the thickness of casing. | Yanxian Wu Shouming Zhong Zhigang Guan Lin Song Kun Li Jiarui Sun Yuqiang Xu Maochuan Tu Chaobin Fan Jinbin Yang | 2023 | Fluid Dynamics & Materials Processing2023，19，1： | 0 |
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