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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Non-Smad pathways in TGF-β signaling显示文摘转变生长因素 -- 尾除了 Smads 利用大量的细胞内部的发信号小径调整细胞的功能的一个宽数组。这些不在经典中, non-Smad 小径被占据 ligand 的受体直接激活到增强,稀释,或不那样调制下游地细胞的回答。这些 non-Smad 小径包括地图 kinase 小径,象 Rho 一样 GTPase 发信号小径,和 phosphatidylinositol-3-kinase/AKT 小径的各种各样的分支。这评论在 non-Smad 小径的分子、生物化学的机制的理解集中于最近的进展。另外,这些 non-Smad 小径的功能也被讨论。 | Ying E Zhang | 2009 | Cell Research2009，19，1： | 67 |
| 3 | Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells显示文摘 | Ma Y Xu Y Xiao Z Yang W Zhang C Song E Du Y Li L | 2006 | 中国生物学文摘2006，20，5： | 58 |
| 4 | MicroRNA-34a inhibits glioblastoma growth by targeting multiple oncogenes显示文摘 | Li Y Guessous F Zhang Y Dipierro C Kefas B Johnson E Marcinkiewicz L Jiang J Yang Y Schmittgen TD Lopes B Schiff D Purow B Abounader R | 2009 | 中国神经肿瘤杂志2009，7，4： | 56 |
| 5 | On the Application of PCA Technique to Fault Diagnosis显示文摘In this paper, we briefly address the application of the standard principal component analysis (PCA) technique to fault detection and identification. Based on an analysis of the existing test statistic, we propose a new test statistic, which is similar to the Hawkin’s TH 2 statistic but without the numerical drawback. In comparison with the SPE index, the threshold setting associated with the new statistic is computationally simpler. Our further study is dedicated to the analysis of fault sensitivity. We consider the off-set and scaling faults, and evaluate the test statistic by viewing its sensitivity to the faults. Our final study focuses on identifying off-set and scaling faults. To this end, two algorithms are proposed. This paper also includes some critical remarks on the application of the PCA technique to fault diagnosis. | DING S ZHANG P DING E YIN S Naik A DENG P GUI W | 2010 | Tsinghua Science and Technology2010，15，2： | 31 |
| 6 | Relationship of Helicobacterpylori eradication with gastric cancer and gastric mucosal histological changes: a 10-year follow-up study显示文摘 | Zhou Liya Lin Sanren Ding Shigang Huang Xuebiao Jin Zhu Cui Rongli Meng Lingmei Li Yuan Zhang Li Guo Changji Xue Yan Yan Xiu'e Zhang Jing | 2014 | Chinese Medical Journal2014，，8： | 28 |
| 7 | Pharmacokinetics and disposition of anlotinib, an oral tyrosine kinase inhibitor, in experimental animal species显示文摘 | Chen-chun ZHONG Feng CHEN Jun-ling YANG Wei-wei JIA Li LI Chen CHENG Fei-fei DU Su-ping ZHANG Chengying XIE Na-ting ZHANG Olajide E OLALEYE Feng-qing WANG Fang XU Li-guang LOU Dong-ying CHEN Wei NIU Chuan LI | 2018 | Acta Pharmacologica Sinica2018，39，6： | 24 |
| 8 | Effects of retinoic acid on proliferation,phenotype and expression of cyclin-dependent kinase inhibitors in TGF-β1-stimulated rat hepatic stellate cells显示文摘AIM To study the molecular mechanisms ofretinoic acid(RA)on proliferation andexpression of cyclin-dependent kinase inhibitors(CKI),i.e.p16,p21 and p27 in cultured rathepatic stellate cells(HSC)stimulated withtransforming growth factor beta 1(TGF-β1).METHODS HSC were isolated from healthy ratlivers and cultured.After stimulated with1 mg/L TGF-β1,subcultured HSC were treatedwith or without 1 nmol/L RA.MTT assay,immunocytochemistry(ICC)for p16,p21,p27and α-smooth muscle actin(α-SMA)protein,insitu hybridization(ISH)for retinoic acidreceptor beta 2(RAR-β2)and p16,p21 and p27mRNA and quantitative image analysis(partially)were performed.RESULTS RA inhibited HSC proliferation(41.50%,P<0.05),decreased the protein levelof α-SMA(55.09%,P<0.05),and induced HSCto express RAR-β2 mRNA.In addition,RAincreased the protein level of p16(218.75%,P<0.05)and induced p21 protein expression;meanwhile,p27 was undetectable by ICC in bothcontrol and RA-treated HSC.However,RA hadno influence on the mRNA levels of p16,p21 orp27 as determined by ISH.CONCLISION Up-regulation of p16 and p21 on post-transcriptional level may contribule, in part to RA inhibition of TGF-β1-initiated rat HSC activation in vitro. | Guang Cun Huang Jin Sheng Zhang Yue E Zhang Department of Pathology School of Basic Medical Sciences,Fudan University.Shanghai 200032,China | 2000 | World Journal of Gastroenterology2000，6，6： | 23 |
| 9 | Study of heteroserum-induced rat liver fibrosis model and its mechanism显示文摘StudyofheteroseruminducedratliverfibrosismodelanditsmechanismHUANGZhiGang,ZHAIWeiRong,ZHANGYueEandZHANGXiuRongSubjecthea... | HUANG Zhi Gang, ZHAI Wei Rong, ZHANG Yue E and ZHANG Xiu Rong | 1998 | World Journal of Gastroenterology1998，4，3： | 22 |
| 10 | In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells显示文摘Adipose-derived stem cells and bone marrow-derived stromal stem cells were co-cultured with untreated or Aβ1-40-treated PC12 cells, or grown in supernatant derived from untreated or Aβ1-40-treated PC12 cells. Analysis by western blot and quantitative real-time PCR showed that protein levels of Nanog, Oct4, and Sox2, and mRNA levels of miR/125a/3p were decreased, while expression of insulin-like growth factor-2 and neuron specific enolase was increased. In comparison, the generation of neuron specific enolase-positive cells was most successful when adipose-derived stem cells were co-cultured with Aβ1-40-treated PC12 cells. Our results demonstrate that adipose-derived stem cells and bone marrow-derived stromal stem cells exhibit trends of neuronal-like cell differentiation after co-culture with Aβ1-40-treated PC12 cells. This process may relate to a downregulation of miR-125a-3p mRNA expression and increased levels of insulin-like growth factor-2 expression. | Jin Zhou Guoping Tian Jing'e Wang Xuefeng Cong Xingkai Wu Siyang Zhang Li Li Bing Xu Feng Zhu Xuedan Luo Jian Han Fengjie Hu | 2011 | Neural Regeneration Research2011，6，19： | 21 |
| 11 | Expression of calreticulin is associated with infiltration of T-cells in stage ⅢB colon cancer显示文摘AIM: To investigate the correlation between expression of calreticulin and infiltration of lymphocytes in stage ⅢB colon cancer. METHODS: Sixty-eight pathologically-confirmed speci-mens were obtained from stage ⅢB (T3N1M0) colon cancer patients who underwent radical resection between January 1999 and May 2002 at the Cancer Center of Sun Yat-Sen University, Guangzhou, China. Immuno-histochemical analysis was performed to show infiltration of lymphocytes and expression of calreticulin in colon cancer. Association between calreticulin expression, infiltration of lymphocytes, and 5-year survival rate of patients was assessed. RESULTS: The expression level of calreticulin was lower in cancer nest than in its adjacent normal epithelium since 61.8% (42/68) of the samples were stained with calreticulin in colon cancer. The expression of calreticulin in colon cancer was associated with the infi ltration of CD45RO+ cells rather than with that of CD3+ cells. In addition, the stronger expression of calreticulin and the higher infiltration of CD3+ and CD45RO+ cells in colon cancer were associated with the higher 5-year survival rate of patients. CONCLUSION: Expression of calreticulin is associated with infiltration of T-cells, which implies that a low expression level of molecular marker may represent a new mechanism underlying immune escape in colon cancer. | Rui-Qing Peng, Ya Ding, Xing Zhang, Yi-Xin Zeng, Xiao- Shi Zhang, State Key Laboratory of Oncology in South China, Biotherapy Center, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Ying-Bo Chen, Zhi-Wei Zhou, State Key Laboratory of Oncology in South China, Department of Gastric and Pancreatic Oncology, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Rong Zhang, State Key Laboratory of Oncology in South China, Department of Endoscopy, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Xing-Juan Yu, Yi-Xin Zeng, State Key Laboratory of Oncology in South China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China | 2010 | World Journal of Gastroenterology2010，16，19： | 16 |
| 12 | Multi-scenario-based hazard analysis of high temperature extremes experienced in China during 1951-2010显示文摘China is physically and socio-economically susceptible to global warming-derived high temperature extremes because of its vast area and high urban population density. This article presents a scenario-based analysis method for high temperature extremes aimed at illustrating the latter's hazardous potential and exposure across China. Based on probability analysis, high temperature extreme scenarios with return periods of 5, 10, 20, and 50 years were designed, with a high temperature hazard index calculated by integrating two differentially-weighted extreme temperature indices (maximum temperature and high temperature days). To perform the exposure analysis, a land use map was employed to determine the spatial distribution of susceptible human activities under the different scenarios. The results indicate that there are two heat-prone regions and a sub-hotspot occupying a relatively small land area. However, the societal and economic consequences of such an environmental impact upon the North China Plain and middle/lower Yangtze River Basin would be substantial due to the concentration of human activities in these areas. | YIN Zhan'e YIN Jie ZHANG Xiaowei | 2013 | Journal of Geographical Sciences2013，23，3： | 16 |
| 13 | Intelligent transportation systems for smart cities:a progress review显示文摘To better meet the challenge of providing effective,low-cost,energy efficient transport services,the concept of intelligent transport systems(ITS) has been proposed and lauded as an innovative and promising solution for next generation transport networks.In this paper,the progress of ITS research around the world is briefly reviewed and current challenges are outlined,thereby offering further insight into ITS development for all researchers in this area. | XIONG Zhang SHENG Hao RONG WenGe COOPER Dave E | 2012 | Science China(Information Sciences)2012，55，12： | 16 |
| 14 | Chimeric antigen receptor-engineered T-cell therapy for liver cancer显示文摘Background: Chimeric antigen receptor-engineered T-cell(CAR-T) therapy is a newly developed immunotherapy used in the treatment of cancers. Because CAR-T therapy has shown great success in treating CD19-positive hematological malignancies, its application has been explored in the treatment of solid tumors, such as liver cancer. In this review, we discuss the immune characteristics of liver cancer, the obstacles encountered during the application of CAR-T therapy, and preclinical and clinical progress in the use of CAR-T therapy in patients with liver cancer.Data sources: The data on CAR-T therapy related to liver cancers were collected by searching Pub Med and the Web of Science databases prior to December 2017 with the keywords 'chimeric antigen receptor','CAR-T', 'liver cancer', 'hepatocellular carcinoma', and 'solid tumor'. Additional articles were identified by manual search of references found in the primary articles. The data for clinical trials were collected by searching Clinical Trials.gov.Results: The liver has a tolerogenic nature in the intrahepatic milieu and its tumor microenvironment significantly affects tumor progression. The obstacles that reduce the efficacy of CAR-T therapy in solid tumors include a lack of specific tumor antigens, limited trafficking and penetration of CAR-T cells to tumor sites, and an immunosuppressive tumor microenvironment. To overcome these obstacles, several strategies have emerged. In addition, several strategies have been developed to manage the side effects of CAR-T, including enhancing the selectivity of CARs and controlling CAR-T activity. To date, no clinical trials of CAR-T therapy against HCC have been completed. However, preclinical studies in vitro and in vivo have shown potent antitumor efficacy. Glypican-3, mucin-1, epithelial cell adhesion molecule, carcinoembryonic antigen, and other targets are currently being studied.Conclusions: The application of CAR-T therapy for liver cancer is just beginning to be explored and more research is needed. However, we are optimistic that CAR-T therapy will offer a new approach for the treatment of liver cancers in the future. | Yang Chen Chang-Yong E Zhi-Wen Gong Shui Liu Zhen-Xiao Wang Yong-Sheng Yang Xue-Wen Zhang | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，4： | 16 |
| 15 | 在血压控制不良的高血压患者中使用数字化干预的家庭和在线血压管理:随机对照试验显示文摘HOME BP试验(The home and online management and evaluation of blood pressure)的目的是评估在初级预防中结合自我监测和自我管理的数字化干预在高血压管理中的作用。研究者纳入英国76个治疗中心经过治疗但血压控制不好(>140/90 mm Hg, 1 mm Hg=0.133 kPa)并能上网的患者622例,进行自动确定主要终点的公开随机对照试验。 | McMa-nus RJ Little P Stuart B Morton K Raftery J Kelly J Bradbury K Zhang J Zhu S Murray E May CR Mair FS Michie S Smith P Band R Ogburn E AllenJ Rice C Nut-tall J Williams B Yardley L 陈嘉睿(译) 叶鹏(审校) | 2021 | 中华高血压杂志2021，29，5： | 15 |
| 16 | Effects of acid deposition on terrestrial ecosystems and their rehabilitation strategies in China显示文摘South China has become the third largest region associated with acid deposition following Europe and North America, the area subject to damage by acid deposition increased from 1.75 million km 2 in 1985 to 2.8 million km 2 in 1993. Acid deposition has caused serious damage to ecosystem. Combined pollution of acid rain and SO 2 showed the obvious multiple effects on crops. Vegetable was more sensitive to acid deposition than foodstuff crops. Annual economic loss of crops due to acid deposition damage in eleven provinces of south China was 4.26 billion RMB Yuan. Acid deposition caused serious damage to forest. Annual economic loss of wood volume was about 1.8 billion RMB Yuan and forest ecological benefit loss 16.2 billion in eleven provinces of south China. Acid deposition in south China was typical “sulfuric acid type”. According to the thoughts of sustainable development, some strategies were brought forward as follows: (1) enhancing environmental management, specifying acid\|controlling region, controlling and abating the total emission amount of SO 2; (2) selecting practical energy technologies of clean coal, for example, cleansing and selecting coal, sulfur\|fixed\|type industrial briqutting, abating sulfur from waste gas and so on; (3) developing other energy sources to replace coal, including water electricity, atomic energy and the new energy such as solar energy, wind energy and so on; (4) in acid deposition region of south China, selecting acid\|resistant type of crop and tree to decrease agricultural losses, planting more green fertilizer crops, using organic fertilizers and liming, in order to improve buffer capacities of soil. | FENG Zong\|wei, MIAO Hong, ZHANG Fu\|zhu, HUANG Yi\|zong (Research Center for Eco\|Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. E\|mail: FZW@mail.rcees.ac.cn) | 2002 | Journal of Environmental Sciences2002，14，2： | 15 |
| 17 | Efficacy of adjuvant XELOX and FOLFOX6 chemotherapy after D2 dissection for gastric cancer显示文摘AIM: To compare the efficacy of capecitabine and oxaliplatin (XELOX) with 5-fluorouracil, folinic acid and oxaliplatin (FOLFOX6) in gastric cancer patients after D2 dissection. METHODS: Between May 2004 and June 2010, patients in our gastric cancer database who underwent D2 dissection for gastric cancer at the First Affiliated Hospital of Sun Yat-Sen University were retrospectively analyzed. A total of 896 patients were enrolled into this study according to the established inclusion and exclusion criteria. Of these patients, 214 received the XELOX regimen, 48 received FOLFOX6 therapy and 634 patients underwent surgery only without chemotherapy. Overall survival was compared among the three groups using Cox regression and propensity score matchedpair analyses. RESULTS: Patients in the XELOX and FOLFOX6 groups were younger at the time of treatment (median age 55.2 years; 51.2 years vs 58.9 years), had more undifferentiated tumors (70.1%; 70.8% vs 61.4%), and more lymph node metastases (80.8%; 83.3% vs 57.7%), respectively. Overall 5-year survival was 57.3% in the XELOX group which was higher than that (47.5%) in the surgery only group (P = 0.062) and that (34.5%) in the FOLFOX6 group (P = 0.022). Multivariate analysis showed that XELOX therapy was an independent prognostic factor (hazard ratio = 0.564, P < 0.001). After propensity score adjustment, XELOX significantly increased overall 5-year survival compared to surgery only (58.2% vs 44.2%, P = 0.025) but not compared to FOLFOX6 therapy (48.5% vs 42.7%, P = 0.685). The incidence of grade 3/4 adverse reactions was similar between the XELOX and FOLFOX6 groups, and more patients suffered from hand-foot syndrome in the XELOX group (P = 0.018). CONCLUSION: Adjuvant XELOX therapy is associated with better survival in patients after D2 dissection, but does not result in a greater survival benefit compared with FOLFOX6 therapy. | Ying Wu Zhe-Wei Wei Yu-Long He Roderich E Schwarz David D Smith Guang-Kai Xia Chang-Hua Zhang | 2013 | World Journal of Gastroenterology2013，19，21： | 14 |
| 18 | Atmospheric particulate pollution of Lanzhou using magnetic measurements显示文摘To differentiate between natural and anthropogenic particulate sources in the atmosphere in Lanzhou City, samples were collected in different sites. The dust flux was calculated and magnetic measurements were conducted. Results show a distinct pattern of variation of dust flux within a year and it agrees with the shifts of atmospheric circulation regime. The magnetic parameters indicate that natural sources are the major components of atmospheric particulate during late spring and early summer, while anthropogenic sources contribute much more during winter months. The data also support the earlier findings that magnetic parameters are effective for differentiating between particulate arising from natural sources such as soil erosion and from anthropogenic sources such as coal combustion. | Chen Fa\|hu, Yang Li\|ping, Wang Jian\|min, Zhang Jia\|wu Institute of Environment & Hazards, Lanzhou University, Lanzhou 730000, China. E\|mail: fhchen@lzu.edu.cn | 1999 | Journal of Environmental Sciences1999，11，3： | 14 |
| 19 | Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat显示文摘 | Hong-Sen Bi Chen Zhang Fang-Fei Nie Bo-Lin Pan E Xiao | 2018 | Chinese Medical Journal2018，，5： | 14 |
| 20 | Quantitative Trait Loci Mapping of Maize Yield and Its Components Under Different Water Treatments at Flowering Time显示文摘干旱或水应力是导致玉米(Zea mays L.) 的一个严重农学的问题在全世界的收益损失。与干旱忍耐引起混血儿是为解决这个问题的一条重要途径。然而,更低的效率和引起混血儿的一个更长的时期是传统的繁殖节目的劣势。到传统的繁殖节目的适用的分子的标记技术能改进繁殖干旱容忍的玉米的效率,这通常被认出。在玉米干旱忍耐的研究为使用提供有用信息,为产量和它的部件印射并且标注量的特点 loci (QTL ) 根据一个混合线性模型的原则在现在的学习被执行。221 recombinant Yuyu 22 的生来的线(RIL ) 在浇得好、强调水的条件下面被种。在以前的处理组,植物很好被灌溉,而那些在 flowering 时间在后者处理组被强调。每遗传型的十植物连续不断地被种那是 3.00 m ??? 隤? ???? ? 跧?? 闦?? ? 郦 ?? ? 釧 ? ? 跧? 釧??? 雧???????? 雧? 吗?? | Gui-He Lu Ji-Hua Tang Jian-Bing Yan Xi-Qing Ma Jian-Sheng Li Shao-Jiang Chen Jian-Cang Ma Zhan-Xian Liu Li-Zhu E Yi-Rong Zhang Jing-Rui Dai | 2006 | Journal of Integrative Plant Biology2006，48，10： | 13 |
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