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您的检索式：作者名="ZHANG Zhihuai"
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| 1 | Circadian rhythms in the growth and reproduction of the brown alga Undaria pinnatifida and gametogenesis under different photoperiods显示文摘Circadian growth rhythm of the juvenile sporophyte of the brown alga Undaria pinnatifida was measured with the computer-aided image analysis system in constant florescent white light under constant temperature (10 ℃). The growth rhythm persisted for 4 d in constant light with a free-running period of 25.6 h. Egg release from filamentous gametophytes pre-cultured in the light-dark regime was evaluated for six consecutive days at fixed time intervals in constant white light and 12 h light per day. Egg release rhythm persisted for 3 d in both regimes, indicating the endogenous nature. Temporal scale of egg release and gametogenesis in 18, 16, 12 and 8 h light per day were evaluated respectively using vegetatively propagated filamentous gametophytes. Egg release occurred 2 h after the onset of dark phase and peaked at midnight. Evaluation of the rates of oogonium formation, egg release or fertilization revealed no significant differences in four light-dark regimes, indicating the great plasticity of sexual reproduction. No photoperiodic effect in gametogenesis in terms of oogonium formation and egg release was found, but fertilization in short days was significantly higher than in long days. Results of this investigation further confirmed the general occurrence of circadian rhythms in intertidal seaweed species. | ZHANG Zhihuai PANG Shaojun | 2007 | Acta Oceanologica Sinica2007，26，4： | 5 |
| 2 | Chromobox homolog 4 overexpression inhibits TNF-α-induced matrix catabolism and senescence by suppressing activation of NF-κB signaling pathway in nucleus pulposus cells显示文摘Intervertebral disc degeneration(IDD)is featured as enhanced catabolism of extracellular matrix(ECM)in the nucleus pulposus(NP),in which tumor necrosis factor-alpha(TNF-α)-related cell senescence is involved.Chromobox homolog protein 4(CBX4)exhibits anti-inflammatory effects and shows promising therapeutic potential.Thus,in the present study,we explore the role of CBX4 in IDD.Immunohistochemistry staining reveals that CBX4 expression is decreased in severe degenerative NP tissues compared to mild degenerative tissues,and real-time PCR and western blot analysis results show that CBX4 expression is downregulated under TNF-αstimulation in NP cells.siRNA and adenoviruses are used to knockdown or overexpress CBX4,respectively.The results demonstrate that CBX4 knockdown augments the catabolism of ECM in human NP cells,while CBX4 overexpression in rat NP cells restores the ECM degradation induced by TNF-α,as illustrated by immunofluorescence and western blot analysis.In addition,transcriptome sequencing results reveal the regulatory effect of CBX4 on the cell cycle,and further western blot analysis and senescence-associatedβ-galactosidase staining assay indicate that CBX4 overexpression alleviates cell senescence in the presence of TNF-α.Moreover,the phosphorylation of p65,which indicates the activation of NF-κB signaling,is measured by western blot analysis and immunofluorescence assay,and the results reveal that CBX4 overexpression reduces the TNF-α-induced increase in the p-p65/p65 ratio.In addition,the effect of CBX4 overexpression in NP cells is suppressed by NF-κB agonist.In summary,our results indicate that CBX4 overexpression can suppress TNF-α-induced matrix catabolism and cell senescence in the NP by inhibiting NF-κB activation.This study may provide new approaches for preventing and treating IDD. | Yangyang Zhang Shuangxing Li Junmin Hong Jiansen Yan Zhengqi Huang Jiajun Wu Zhihuai Deng Tianyu Qin Kang Xu Wei Ye | 2022 | Acta Biochimica et Biophysica Sinica2022，54，7： | 2 |
| 3 | Rosuvastatin suppresses TNF-α-induced matrix catabolism,pyroptosis and senescence via the HMGB1/NF-κB signaling pathway in nucleus pulposus cells显示文摘Intervertebral disc degeneration is mainly caused by irregular matrix metabolism in nucleus pulposus cells and involves inflammatory factors such as TNF-α.Rosuvastatin,which is widely used in the clinic to reduce cholesterol levels,exerts anti-inflammatory effects,but whether rosuvastatin participates in IDD remains unclear.The current study aims to investigate the regulatory effect of rosuvastatin on IDD and the potential mechanism.In vitro experiments demonstrate that rosuvastatin promotes matrix anabolism and suppresses catabolism in response to TNF-αstimulation.In addition,rosuvastatin inhibits cell pyroptosis and senescence induced by TNF-α.These results demonstrate the therapeutic effect of rosuvastatin on IDD.We further find that HMGB1,a gene closely related to cholesterol metabolism and the inflammatory response,is upregulated in response to TNF-αstimulation.HMGB1 inhibition or knockdown successfully alleviates TNF-α-induced ECM degradation,senescence and pyroptosis.Subsequently,we find that HMGB1 is regulated by rosuvastatin and that its overexpression abrogates the protective effect of rosuvastatin.We then verify that the NF-κB pathway is the underlying pathway regulated by rosuvastatin and HMGB1.In vivo experiments also reveal that rosuvastatin inhibits IDD progression by alleviating pyroptosis and senescence and downregulating HMGB1 and p65.This study might provide new insight into therapeutic strategies for IDD. | Weijian Chen Zhihuai Deng Jianxiong Zhu Liang Yuan Shuangxing Li Yangyang Zhang Jiajun Wu Zhengqi Huang Tianyu Qin Wei Ye | 2023 | Acta Biochimica et Biophysica Sinica2023，55，5： | 0 |
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