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| 1 | Inhibition of cyclooxygenase-2 activity in subchondral bone modifies a subtype of osteoarthritis显示文摘Osteoarthritis(OA) causes the destruction of joints. Its pathogenesis is still under investigation, and there is no effective diseasemodifying therapy. Here, we report that elevated cyclooxygenase-2(COX-2) expression in the osteocytes of subchondral bone causes both spontaneous OA and rheumatoid arthritis(RA). The knockout of COX-2 in osteocytes or treatment with a COX-2 inhibitor effectively rescues the structure of subchondral bone and attenuates cartilage degeneration in spontaneous OA(STR/Ort)mice and tumor necrosis factor-α transgenic RA mice. Thus, elevated COX-2 expression in subchondral bone induces both OAassociated and RA-associated joint cartilage degeneration. The inhibition of COX-2 expression can potentially modify joint destruction in patients with arthritis. | Manli Tu Mi Yang Nanxi Yu Gehua Zhen Mei Wan Wenlong Liu Baochao Ji Hairong Ma Qiaoyue Guo Peijian Tong Li Cao Xianghang Luo Xu Cao | 2019 | Bone Research2019，7，3： | 5 |
| 2 | γ-Aminobutyric acid transporter (GAT1) overexpression in mouse affects the testicular morphology显示文摘γ-Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function. γ-Aminobutyric acid transporter was implicated to be involved in this process. However, the potential role of γ-aminobutyric transporter in testis has not been explored. In this study, we investigated the existence of mouse γ-aminobutyric acid transporter subtype I (mGAT1) in testis. Wild-type and transgenic mice, which overexpressing mGAT1 in a variety of tissues, especially in testis, were primarily studied to approach the profile of mGAT1 in testis. Mice with overexpressed mGAT1 develop normally but with reduced mass and size of testis as compared with wild-type. Testicular morphology of transgenic mice exhibited overt abnormalities including focal damage of the spermatogenic epithelium accompanied by capillaries proliferation and increased diameter of seminiferous tubules lumen. Reduced number of spermatids was also found in some seminiferous tubules. Our results clearly demonstrate the presence of GAT1 in mouse testis and imply that GAT1 is possibly involved in testicular function. | MA YING HUA JIA HUA HU XIAO GANG ZHOU ZHEN TONG MEI JIAN FEI LI HE GUO(Shanghai Institute of Cell Biology, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China) | 2000 | Cell Research2000，10，1： | 4 |
| 3 | Preparation, characterization of MCM-56 and catalytic activity in one-step synthesis of MIBK from acetone显示文摘 | Piao Ping Yang Jian Feng Yu Zhen Lü Wang Mei Ping Xu Qing Sheng Liu Xu Wei Yang Tong Hao Wu | 2004 | Catalysis Communications2004，，2： | 1 |
| 4 | Construction, characterization and application of a genomewide promoter library in Saccharomyces cerevisiae显示文摘倡导者们是批评元素控制基因表示,但是能在各种各样的生长条件下面不同地表现。这里,我们报导一个染色体宽的倡导者图书馆,在 Saccharomyces cerevisiae 的每个本国的倡导者在被克隆的构造黄在上游荧光灯蛋白质(YFP ) 记者基因。九个图书馆任意地在细菌被定义并且集合。倡导者的产生水池能被准备并且也作为 centromeric plasmids 转变了成酵母紧张或集成于在酶的处理之上的一个 genomic 地点。使用荧光激活房间排序,我们基于 YFP 荧光紧张分类酵母紧张并且任意地把全部图书馆划分了成 12 箱,代表对强壮的倡导者弱。几个强壮的倡导者从最活跃的箱被识别,他们的活动是在不同生长条件下面的 assayed。最后,这些倡导者被使用在木糖利用驱使基因的表示改进发酵效率。一起,这个图书馆能提供一个快答案在自定义生长条件下面识别并且利用需要的倡导者。 | Ting Yuan Yakun Guo Junkai Dong Tianyi Li Tong Zhou Kaiwen Sun Mei Zhang Qingyu Wu Zhen Xie Yizhi Cai Limin Cao Junbiao Dai | 2017 | Frontiers of Chemical Science and Engineering2017，11，1： | 1 |
| 5 | Transgenic mice overexpressingγ-aminobutyric acid transporter subtypeⅠ develop obesity显示文摘Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity. | MA YING HUA JIA HUA HU XIAO GANG ZHOU RUO WANG ZENG ZHEN TONG MEI JIAN FEI LI HE GUO Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of | 2000 | Cell Research2000，10，4： | 0 |
| 6 | Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping显示文摘Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment com-bined with high chloroform Carnory’s fixative solution from cells of the testes of domestic pigs. Comparison in the division index and length of pachytene bivalents with metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87-5.98) times longer than those of the latter. Comparative studies on chromomere maps of bivalents and mitotic chromosomal G-bands were conducted by using the chromo-some 12 as a example. Sex vesicle and various shapes of synaptic sex chromosomes have been observed.Two-color PRimed IN Situ (PRINS) labeling has been conducted successfully on pachytene bivalents of pigs. | BANG LIU, GENG LIN JIN, SHU HONG ZHAO, MEI YU, TONG AN XIONG, ZHONG ZHEN PENG, Km LILaboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China | 2002 | Cell Research2002，12，5： | 0 |
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