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| 1 | Construction of cytopathic PK-15 cell model of classical swine fever virus显示文摘No cytopathic effect (CPE) can be observed on classical swine fever virus (CSFV) infected cell culture in vitro. This brings an obstacle to the researches on reciprocity between CSFV and host cells. Based on the construction of full-length genomic infectious Cdna clone of Chinese CSFV standard virulent Shimen strain, partial deletion is intro- duced into genomic Cdna to obtain a 7.5 kb subgenomic Cdna. A new subgenomic CSFV is derived from transfection with the subgenomic Cdna on PK-15 cells pre-infected by CSFV Shimen virus. Typical CPE induced by this subgenomic virus is observed on PK-15 cells. Coexistence of wild- type and subgenomic virus in cytopathic cell culture is dem- onstrated by RT-PCR detection in cytopathic cells. For conclusion, the construction of cytopathic cell model exploited a new way for researches on the molecular mechanism of CSFV pathogenesis. | WU Haixiang, ZHANG Chuyu, ZHENG Congyi, WANG Jiafu, PAN Zishu, LI Lei, CAO Shen & Yl GuanghuiInstitute of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaCorrespondence should be addressed to Zhang Chuyu (e-mail: avlab@ whu.edu.cn) | 2003 | Chinese Science Bulletin2003，48，9： | 1 |
| 2 | Path Analysis of Energy-Saving Technology in Yangtze River Basin Based on Multi-Objective and Multi-Parameter Optimisation显示文摘The Yangtze River Basin in China is characterised by hot-and cold-humid climates in summer and winter, respectively. Thus, increased demand for heating and cooling energy according to the season, as well as poor indoor thermal comfort, are inevitable. To overcome this problem, this study focused on the influence of passive design and heating, ventilation, and air conditioning equipment performance on the energy performance of residential buildings, and explored potential energy-saving technology paths involving passive design and improved coefficient of performance through a multi-objective and multi-parameter optimisation technique. A large-scale questionnaire survey covering a typical city was first conducted to identify family lifestyle patterns regarding time spent at home, family type, air conditioner use habits, indoor thermal comfort, etc. Then, the actual heating and cooling energy consumption and information of model building were determined for this region. Subsequently, the design parameters of an individual building were simulated using Energyplus to investigate the cooling and heating energy consumption for a typical residential building with an air conditioner. The results indicated an improvement of approximately 30% in energy efficiency through optimisation of the external-wall insulation thickness and the external-window and shading performance, and through use of appropriate ventilation technology. Thus, a multi-objective and multi-parameter optimisation model was developed to achieve comprehensive optimisation of several design parameters. Experimental results showed that comprehensive optimisation could not only reduce cooling and heating energy consumption, but also improve the thermal comfort level achieved with a non-artificial cooling and heating source. Finally, three energy-saving technology paths were formulated to achieve a balance between indoor thermal comfort improvement and the target energy efficiency(20 kWh/(m2?a)). The findings of this study have implications for the future design of buildings in the Yangtze River Basin, and for modification of existing buildings for improved energy efficiency. | MENG Xiangzhong YU Wei ZHENG Chuyu WANG Di CAO Xinyun | 2019 | Journal of Thermal Science2019，28，6： | 1 |
| 3 | Retrospective Analysis of the Duration of Hospitalization of Imported Patients Infected with SARS-CoV-2 in Guangzhou, 2021显示文摘The pandemic of coronavirus disease 2019 is“not over,”in fact,the“dynamic clearing”policy for SARS-CoV-2 control and prevention in China has been firmly enforced.This study aimed to analyze the clinical symptoms and dynamic viral RNA changes in 2021 at Guangzhou Eighth People’s Hospital.This study showed that 31.4%of the patients(695/2212)tested negative for viral RNA from admission to the final release from quarantine.Of all negative cases,86.5%(601/695)remained in the hospital for no more than 5 days and were asymptomatic or mild.Among the remaining 402 patients who stayed for no more than 5 days,76.4%(307/402)were viral RNA retest positive during the isolation stage.However,96.4%of the peak viral RNA(296/307)was over Ct=33 cycles during the isolation stage. | Zhiwei Xie Guofang Tang Lu Li Jingrong Shi Qingxin Gan Xiaowen Zheng Jingyan Tang Huimin Zeng Chuyu Zhang Sisi Cheni Jianping Cui Zishi Lin Lihua Lin Youxia Li Jinxin Liu Feng Li Fengyu Hui Xiaoping Tang Xilong Deng | 2023 | Infectious Diseases & Immunity2023，3，1： | 0 |
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