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| 1 | Genome-and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus显示文摘Seed oil content(SOC)is a highly important and complex trait in oil crops.Here,we decipher the genetic basis of natural variation in SOC of Brassica napus by genome-and transcriptome-wide association studies using 505 inbred lines.We mapped reliable quantitative trait loci(QTLs)that control SOC in eight environments,evaluated the effect of each QTL on SOC,and analyzed selection in QTL regions during breeding.Six-hundred and ninety-two genes and four gene modules significantly associated with SOC were identified by analyzing population transcriptomes from seeds.A gene prioritization framework,POCKET(prioritizing the candidate genes by incorporating information on knowledge-based gene sets,effects of variants,genome-wide association studies,and transcriptome-wide association studies),was implemented to determine the causal genes in the QTL regions based on multi-omic datasets.A pair of homologous genes,BnPMT6s,in two QTLs were identified and experimentally demonstrated to negatively regulate SOC.This study provides rich genetic resources for improving SOC and valuable insights toward understanding the complex machinery that directs oil accumulation in the seeds of B.napus and other oil crops. | Shan Tang Hu Zhao Shaoping Lu Liangqian Yu Guofang Zhang Yuting Zhang Qing-Yong Yang Yongming Zhou Xuemin Wang Wei Ma Weibo Xie Liang Guo | 2021 | Molecular Plant2021，14，3： | 6 |
| 2 | Characteristics of SARS-CoV-2 Delta variant-infected individuals with intermittently positive retest viral RNA after discharge显示文摘SARS-CoV-2-infected patients will be discharged when viral ribonucleic acid(RNA)is consecutively negative at least twice over a 24-h interval.However,we have demonstrated that positive retest viral RNA,a particular clinical phenomenon initially observed in discharged SARS-CoV-2-infected patients in early 2020[1,2],is the occasional reoccurrence of Polymerase Chain Reaction(PCR)-detectable viral RNA but not reinfection[3]. | Lu Li Jingyan Tang Zhiwei Xie Qingxin Gan Guofang Tang Zhongwei Hu Huimin Zeng Jingrong Shi Jiaojiao Li Yan Li Changwen Ke Min Kang Dan Liang Huan Lu Yuwei Tong Xilong Deng Jinxin Liu Hongzhou Lu Fuxiang Wang Fengyu Hu Feng Li Nanshan Zhong Xiaoping Tang | 2022 | National Science Review2022，9，10： | 3 |
| 3 | A rapid and efficient method for primary culture of human adipose-derived stem cells显示文摘 | Guofang Zeng Kuan Lai Jin Li Yaqin Zou Haili Huang Jie Liang Xudong Tang Jing Wei Peihua Zhang | 2013 | Organogenesis2013，，4： | 2 |
| 4 | Association of plasminogen activator inhibitor-1 4G/5G promoter polymorphism with recurrent cerebral infarction in China’s North Jiangsu Province显示文摘BACKGROUND: Many international studies have shown that plasminogen activator inhibitor-1 (PAI-1) 4G/5G promoter polymorphism does not increase the risk for cerebral infarction. OBJECTIVE: Using PCR methodology and agarose electrophoresis to detect PAI-1 4G/5G promoter polymorphism in patients with recurrent cerebral infarction in the North Jiangsu Province of China, and to compare results with healthy subjects and patients with first-occurrence cerebral infarction in the same region. DESIGN, TIME AND SETTING: Non-randomized, concurrent, control trial. A total of 122 cerebral infarction patients were admitted to Xuzhou Medical College Hospital's Department of Neurology and Xuzhou Central Hospital’s Department of Neurology between July 2003 and August 2006. PARTICIPANTS: The patients consisted of 63 males and 59 females, aged (62 ± 10) years. They were divided into first-occurrence (n = 58) and recurrence (n = 64) groups. In addition, 50 healthy subjects that underwent physical examination in the outpatient department, including 26 males and 24 females, aged (60 ±12) years, were selected as controls. METHODS AND MAIN OUTCOME MEASURES: PAI-1 4G/5G promoter polymorphism was detected and analyzed using PCR methodology and agarose electrophoresis. RESULTS: Significant differences were determined in terms of genotypic frequency and allele frequency of PAI-1 4G/5G promoter polymorphism, in patients with first-occurrence or recurrent cerebral infarction, when compared with healthy subjects (P < 0.05). There was, however, no significant difference between the first-occurrence and recurrence groups (P > 0.05). CONCLUSION: PAI-1 4G/5G promoter polymorphism is genetic risk factor for cerebral infarction in China. However, it may be associated with recurrence of cerebral infarction in patients from the North Jiangsu Province of China. | Deqin Geng Jijun Zhu Guofang Chen Xianbi Tang Qiaoyun Yang Jizhen Li Fumin Liu | 2008 | Neural Regeneration Research2008，3，7： | 1 |
| 5 | Vascular Endothelial Growth Factor‑Recruiting Nanofiber Bandages Promote Multifunctional Skin Regeneration via Improved Angiogenesis and Immunomodulation显示文摘Tissue injury leads to gradients of chemoattractants,which drive multiple processes for tissue repair,including the inflam-matory response as well as endogenous cell recruitment.However,a limited time window for the gradients of chemoattract-ants as well as their poor stability at the injury site may not translate into healthy tissue repair.Consequently,intelligent multifunctional scaffolds with the capability to stabilize injury-induced cytokines and chemokines hold great promise for tissue repair.Vascular endothelial growth factor(VEGF)plays a significant role in wound healing by promoting angiogen-esis.The overarching objective of this research was to develop intelligent multifunctional scaffolds with the capability to endogenously recruit VEGF and promote wound healing via angiogenic and immunomodulatory dual functions.Prominin-1-derived peptide(PR1P)was encapsulated into electrospun poly(L-lactide-coglycolide)/gelatin(P/G)-based bandages.The sustained release of PR1P recruited VEGF in situ,thereby stabilizing the protein concentration peak in vivo and affording a reparative microenvironment with an adequate angiogenic ability at the wound site.Meanwhile,PR1P-recruited VEGF-induced macrophage reprogramming towards M2-like phenotypes further conferred immunomodulatory functions to the bandages.These dual functions of proangiogenesis and immunomodulation formed a cascade amplification,which regulated matrix metalloproteinases(MMP-9)as well as inflammatory factors(nuclear factor(NF)-κb,tumor necrosis factor(TNF)-α)in the wound microenvironment via the VEGF/macrophages/microenvironment axis.Consequently,the bandages realized multifunctional regeneration in splinted excisional wounds in rats,with or without diabetes,affording a higher skin append-age neogenesis,sensory function,and collagen remodeling.Conclusively,our approach encompassing in situ recruitment of VEGF at the injury site with the capability to promote immunomodulation-mediated tissue repair affords a promising avenue for scarless wound regeneration,which may also have implications for other tissue engineering disciplines. | Yi Chen Zhengchao Yuan Weiyan Sun Muhammad Shafiq Jun Zhu Jiafei Chen Hai Tang Ling Hu Weikang Lin Yanxi Zeng Long Wang Lei Zhang Yunlang She Hui Zheng Guofang Zhao Dong Xie Xiumei Mo Chang Chen | 2023 | Advanced Fiber Materials2023，5，1： | 1 |
| 6 | Preparation and Characterization of High Quality Diamond Films by DC ArcPlasma Jet CVD Method显示文摘OneoftheimpoftantprogressesinCVDdiamondresearchesisthepreparationofopticalgradetransparentdiamondfilmsinrecentyears[l-2].ThephysicalandchemicalpropertiesofthesefilmsarenearlythesameasthatofthenaturaltypeIladiamond,soopticalgradediamondfilmshavepotent... | Guofang Zhong Fazheng Shen Fanxiu Lu Weizhong Tang(Material Science and Engineering School, University of Science and Technology’ Beijing, Beijing 100083, China) | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，4： | 1 |
| 7 | Correction to:Vascular Endothelial Growth Factor‑Recruiting Nanofiber Bandages Promote Multifunctional Skin Regeneration via Improved Angiogenesis and Immunomodulation显示文摘Correction to:Advanced Fiber Materials http://gffzzd3cc09b8251d45dfs99vxoppcbuqn6oq6.ffgz.tsg.suse.edu.cn/10.1007/s42765-022-00226-8 In this article the author name Muhammad Shafiq was incorrectly written as Shafiq Muhammad.The original article has been corrected. | Yi Chen Zhengchao Yuan Weiyan Sun Muhammad Shafiq Jun Zhu Jiafei Chen Hai Tang Ling Hu Weikang Lin Yanxi Zeng Long Wang Lei Zhang Yunlang She Hui Zheng Guofang Zhao Dong Xie Xiumei Mo Chang Chen | 2023 | Advanced Fiber Materials2023，5，2： | 0 |
| 8 | Effects of spectral resolution and temporal envelope periodicity to musical pitch discrimination显示文摘The main goal of this study was to systematically investigate the relative contribution of spectral resolution and temporal envelope periodicity to musical pitch discrimination. Stimuli from four instruments(clarinet,trumpet,piano and violin) and synthetic complex tone were utilized.A noise-excited vocoder was used to control the spectral resolution and temporal envelope periodicity of the musical stimuli.Ten normal-hearing subjects were recruited for the study.Psychoacoustic experiments on pitch discrimination were carried out.Results showed that the spectral cue was important for musical pitch discrimination.Relative good performance could be achieved when there were 16 frequency channels.No clear effect was found for the temporal envelope periodicity to pitch discrimination. | PING Lichuan YUAN Meng TANG Guofang FENG Haihong | 2012 | Chinese Journal of Acoustics2012，31，4： | 0 |
| 9 | Place-pitch perception in electrical hearing with post-lingually deafened cochlear implant users显示文摘The main goal of this study was to systematically investigate place-pitch perception in electrical hearing and the relative relationship between place-pitch perception ability,speech understanding and musical pitch discrimination by cochlear implant(CI) users.Electrode pitch ranking test was carried out to evaluate the place-pitch perception ability of CI users. Four post-lingually deafened CI users were recruited.They also participated in the speech recognition test and musical pitch discrimination test.Results showed that place pitch were generally ordered from apical to basal electrodes.The apical electrodes were judged lower in pitch than basal electrodes.Large individual difference was found.Comparing pitch and speech performance,the speech recognition result was related to the place-pitch perception ability of CI users,but this relationship was limited by the ceiling effects.However,a correlative relationship was found between musical pitch discrimination result and place-pitch ability of CI users.It indicated that the current signal processing of CI system can provide sufficient information for speech understanding but not for music perception of CI users.To a certain extent,music perception of CI users was determined by their place-pitch abilities. | PING Lichuan YUAN Meng TANG Guofang FENG Haihong | 2012 | Chinese Journal of Acoustics2012，31，4： | 0 |
| 10 | A peptide chain release factor 2a gene regulates maize kernel development by modulating mitochondrial function显示文摘Mitochondrial protein translation that is essential for aerobic energy production includes four essential steps of the mitochondrial ribosome cycle,namely,initiation,elongation,termination of the polypeptide,and ribosome recycling.Translation termination initiates when a stop codon enters the A site of the mitochondrial ribosome where it is recognized by a dedicated peptide release factor(RF).However,RFs and mechanisms involved in translation in plant mitochondria,especially in monocotyledons,remain largely unknown.Here,we identified a crumpled kernel(crk5 allele)mutant,with significantly decreased kernel size,100-kernel weight,and an embryo-lethal phenotype.The Crk5 allele was isolated using map-based cloning and found to encode a mitochondrial localization RF2a.As it is an ortholog of Arabidopsis mitochondrial RF2a,we named the gene ZmmtRF2a.ZmmtRF2a is missing the 5th–7th exons in the crk5 resulting in deletion of domains containing motifs GGQ and SPF that are essential for release activity of RF,mitochondrial ribosome binding,and stop codon recognition.Western blot and qRT-PCR analyses indicate that the crk5 mutation results in abnormal mitochondrion structure and function.Intriguingly,we observed a feedback loop in the crk5 with up-regulated transcript levels detected for several mitochondrial ribosome and mitochondrial-related components,in particular mitochondrial complexes CI,CIV,and a ribosome assembly related PPR.Together,our data support a crucial role for ZmmtRF2a in regulation of mitochondrial structure and function in maize. | Hui Zhang Yijian Feng Kunyang Song Guofang Li Jiao Jin Jingjing Gao Yongtian Qin Hongqiu Wang Jinpeng Cheng Zonghua Liu Jihua Tang Zhiyuan Fu | 2023 | The Crop Journal2023，11，6： | 0 |
| 11 | ZmCYP90D1 regulates maize internode development by modulating brassinosteroid-mediated cell division and growth显示文摘Plant height(PH)is associated with lodging resistance and planting density,which is regulated by a complicated gene network.In this study,we identified a spontaneous dwarfing mutation in maize,m30,with decreased internode number and length but increased internode diameter.A candidate gene,ZmCYP90D1,which encodes a member of the cytochrome P450 family,was isolated by map-based cloning.ZmCYP90D1 was constitutively expressed and showed highest expression in basal internodes,and its protein was targeted to the nucleus.A G-to-A substitution was identified to be the causal mutation,which resulted in a truncated protein in m30.Loss of function of ZmCYP90D1 changed expression of hormoneresponsive genes,in particular brassinosteroid(BR)-responsive genes which is mainly involved in cell cycle regulation and cell wall extension and modification in plants.The concentration of typhasterol(TY),a downstream intermediate of ZmCYP90D1 in the BR pathway,was reduced.A haplotype conferring dwarfing without reducing yield was identified.ZmCYP90D1 was inferred to influence plant height and stalk diameter via hormone-mediated cell division and cell growth via the BR pathway. | Canran Sun Yang Liu Guofang Li Yanle Chen Mengyuan Li Ruihua Yang Yongtian Qin Yongqiang Chen Jinpeng Cheng Jihua Tang Zhiyuan Fu | 2024 | The Crop Journal2024，12，1： | 0 |
| 12 | A novel nomogram based on routine clinical indicators for screening for Wilson's disease显示文摘Background and aims:There is currently no single model for predicting Wilson's disease(WD).We aimed to create a nomogram using daily clinical parameters to improve the accuracy of WD diagnosis in patients with abnormal liver function.Methods:Between July 2016 and December 2020,we identified 90 WD patients with abnormal liver function who had homozygous or compound heterozygous mutations in the ATP7B gene.The control group included 128 patients with similar liver function but no WD during the same time period.To create a nomogram,we screened potential predictive variables using the least absolute shrinkage and selection operator model and multivariate logistic regression.Results:We developed a nomogram for screening for WD based on six predictive factors:serum copper,direct bilirubin,uric acid,cholinesterase,prealbumin,and reticulocyte percentage.In the training cohort,the area under curve(AUC)of the nomogram reached 0.967(95%confidence interval(CI)0.946e0.988),while the area under the precision-recall curve was 0.961.Based on the optimal cutpoint of 213.55,our nomogram performed well,with a sensitivity of 96%and a specificity of 87%.In the validation cohort,the AUC of the nomogram was as high as 0.991(95%CI 0.970e1.000).Conclusions:We developed a nomogram that can predict the risk of WD prior to the detection of serum ceruloplasmin or urinary copper,greatly increasing screening efficiency for patients with abnormal liver function. | Jiahui Pang Shuru Chen Weiqiang Gan Guofang Tang Yusheng Jie Zhanyi Li Yutian Chong Youming Chen Jiao Gong Xinhua Li Yongyu Mei | 2023 | Liver Research2023，7，1： | 0 |
| 13 | Simultaneous gap arthroplasty combined with distraction osteogenesis in the treatment of unilateral temporo-mandibular joint ankylosis with micrognathia deformity显示文摘 | Hong Bo Yu Steve Guofang Shen Shi Lei Zhang Xu Dong Wang You Sheng Tang | 2008 | 中国口腔颌面外科杂志2008，6，B05： | 0 |
| 14 | Retrospective Analysis of the Duration of Hospitalization of Imported Patients Infected with SARS-CoV-2 in Guangzhou, 2021显示文摘The pandemic of coronavirus disease 2019 is“not over,”in fact,the“dynamic clearing”policy for SARS-CoV-2 control and prevention in China has been firmly enforced.This study aimed to analyze the clinical symptoms and dynamic viral RNA changes in 2021 at Guangzhou Eighth People’s Hospital.This study showed that 31.4%of the patients(695/2212)tested negative for viral RNA from admission to the final release from quarantine.Of all negative cases,86.5%(601/695)remained in the hospital for no more than 5 days and were asymptomatic or mild.Among the remaining 402 patients who stayed for no more than 5 days,76.4%(307/402)were viral RNA retest positive during the isolation stage.However,96.4%of the peak viral RNA(296/307)was over Ct=33 cycles during the isolation stage. | Zhiwei Xie Guofang Tang Lu Li Jingrong Shi Qingxin Gan Xiaowen Zheng Jingyan Tang Huimin Zeng Chuyu Zhang Sisi Cheni Jianping Cui Zishi Lin Lihua Lin Youxia Li Jinxin Liu Feng Li Fengyu Hui Xiaoping Tang Xilong Deng | 2023 | Infectious Diseases & Immunity2023，3，1： | 0 |
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