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| 1 | Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella显示文摘Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design. | CHEN CongLin SUN XiaoYun LIAO LanJie LUO ShaoXiang LI ZhouQuan ZHANG XiaoHua WANG YaPing GUO QionLin FANG Qin DAI HePing | 2013 | Science China(Life Sciences)2013，56，1： | 6 |
| 2 | Formation of nanocrystalline graphite in polymer-derived SiCN by polymer infiltration and pyrolysis at a low temperature显示文摘The microstructure of polymer-derived ceramics(PDCs)was closely related to processing.This study demonstrated that SiCN matrix prepared by polymer infiltration and pyrolysis(PIP)at 900℃ inside a Si_(3)N_(4) whisker(Si_(3)N_(4w))preform with submicro-sized pores differed from its powder-consolidated analogue in both the content and structure of free carbon.Chemical analysis showed that PIP process had a higher free carbon yield.Raman spectroscopy and transmission electron microscopy(TEM)observation discovered a higher graphitization degree of free carbon and the existence of nanocrystalline graphite in SiCN matrix.Dielectric properties of Si_(3)N_(4w)/SiCN composites were greatly enhanced when volume fraction of SiCN matrix reached 24.5%due to dielectric percolation caused by highly-lossy free carbon.Reconsolidation of hydrocarbon released during pyrolysis by gas-state carbonization in Si_(3)N_(4) whisker preform was supposed to account for the high yield and graphitization degree of free carbon in PIP process. | Mingxing LI Laifei CHENG Fang YE Conglin ZHANG Jie ZHOU | 2021 | Journal of Advanced Ceramics2021，10，6： | 1 |
| 3 | Characterization and removal of dissolved organic matter (DOM) from landfill leachate rejected by nanofiltration显示文摘 | Long Zhang Aimin Li Yufei Lu Lei Yan Sheng Zhong Conglin Deng | 2008 | Waste Management2008，，3： | 1 |
| 4 | Characterization and removal of dissolved organic matter (DOM) from landfill leachate rejected by nanofiltration显示文摘 | Long Zhang Aimin Li Yufei Lu Lei Yan Sheng Zhong Conglin Deng | 2008 | Waste Management2008，，3： | 1 |
| 5 | CIPK7 is involved in cold response by interacting with CBL1 in Arabidopsis thaliana显示文摘 | HUANG Conglin DING Shuo ZHANG Hua | 2011 | Plant Science2011，，181： | 1 |
| 6 | Microstructures and properties of zirconium-702 irradiated by high current pulsed electron beam显示文摘 | Cai Jie Peng L Zhang Conglin | 2015 | Nuclear Instruments and Methods in Physics Research B2015，358，： | 1 |
| 7 | CIPK7is involved in cold response by interacting with CBL1in Arabidopsis thaliana显示文摘 | Huang Conglin Ding S Zhang H | 2011 | Plant Science2011，，181： | 1 |
| 8 | CIPK7 is involved in cold response by interacting with CBL1 in Arabidopsis thaliana显示文摘 | Conglin Huang Shuo Ding Hua Zhang Hang Du Lizhe An | 2011 | Plant Science2011，，1： | 1 |
| 9 | Review of the evolution and prevention of friction,wear,and noise for water-lubricated bearings used in ships显示文摘With the development of green tribology in the shipping industry,the application of water lubrication gradually replaces oil lubrication in stern bearings and thrust bearings.In terms of large-scale and high-speed ships,water-lubricated bearings with high performance are more strictly required.However,due to the lubricating medium,water-lubricated bearings have many problems such as friction,wear,vibration,noise,etc.This review focuses on the performance of marine water-lubricated bearings and their failure prevention mechanism.Furthermore,the research of marine water-lubricated bearings is reviewed by discussing its lubrication principle,test technology,friction and wear mechanism,and friction noise generation mechanism.The performance enhancement methods have been overviewed from structure optimization and material modification.Finally,the potential problems and the perspective of water-lubricated bearings are given in detail. | Zhuo ZHANG Wu OUYANG Xingxin LIANG Xinping YAN Chengqin YUAN Xincong ZHOU Zhiwei GUO Conglin DONG Zhenglin LIU Yong JIN Jinhua XIAO | 2024 | Friction2024，12，1： | 0 |
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