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| 1 | The material and biological characteristics of osteoinductive calcium phosphate ceramics显示文摘The discovery of osteoinductivity of calcium phosphate(Ca-P)ceramics has set an enduring paradigm of conferring biological regenerative activity to materials with carefully designed structural characteristics.The unique phase composition and porous structural features of osteoinductive Ca-P ceramics allow it to interact with signaling molecules and extracellular matrices in the host system,creating a local environment conducive to new bone formation.Mounting evidence now indicate that the osteoinductive activity of Ca-P ceramics is linked to their physicochemical and three-dimensional structural properties.Inspired by this conceptual breakthrough,many laboratories have shown that other materials can be also enticed to join the rank of tissue-inducing biomaterials,and besides the bones,other tissues such as cartilage,nerves and blood vessels were also regenerated with the assistance of biomaterials.Here,we give a brief historical recount about the discovery of the osteoinductivity of Ca-P ceramics,summarize the underlying material factors and biological characteristics,and discuss the mechanism of osteoinduction concerning protein adsorption,and the interaction with different types of cells,and the involvement of the vascular and immune systems. | Zhurong Tang Xiangfeng Li Yanfei Tan Hongsong Fan Xingdong Zhang | 2018 | Regenerative Biomaterials2018，5，1： | 20 |
| 2 | Vascular restoration therapy and bioresorbable vascular scaffold显示文摘This article describes the evolution of minimally invasive intervention technologies for vascular restoration therapy from early-stage balloon angioplasty in 1970s,metallic bare metal stent and metallic drug-eluting stent technologies in 1990s and 2000s,to bioresorbable vascular scaffold(BVS)technology in large-scale development in recent years.The history,the current stage,the challenges and the future of BVS development are discussed in detail as the best available approach for vascular restoration therapy.The criteria of materials selection,design and processing principles of BVS,and the corresponding clinical trial results are also summarized in this article. | Yunbing Wang Xingdong Zhang | 2014 | Regenerative Biomaterials2014，1，1： | 10 |
| 3 | The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic显示文摘Osteoinductivity of porous calcium phosphate(CaP)ceramics has been widely investigated and confirmed,and it might be attributed to the rapid formation of the vascular networks after in vivo implantation of the ceramics.In this study,to explore the vascularization mechanism within the CaP ceramics,the migration and differentiation of bone marrow-derived mesenchymal stem cells(BMSCs)under the stimulation of porous biphasic calcium phosphate(BCP)ceramic with excellent osteoinductivity were systematically investigated.The results indicated that the directional migration of BMSCs toward BCP ceramic occurred when evaluated by using a transwell model,and the BMSCs migration was enhanced by the seeded macrophages on the ceramic in advance.Besides,by directly culturing BMSCs on BCP ceramic discs under both in vitro and in vivo physiological environment,it was found that the differentiation of BMSCs toward vascular endothelial cells(VECs)happened under the stimulation of BCP ceramic,as was confirmed by the up-regulated gene expressions and protein secretions of VECs-related characteristic factors,including kinase insert domain receptor,von willebrand factor,vascular cell adhesion molecule-1 and cadherin 5 in the BMSCs.This study offered a possibility for explaining the origin of VECs during the rapid vascularization process after in vivo implantation of porous CaP ceramics and could give some useful guidance to reveal the vascularization mechanism of the ceramics. | Ying Chen Jing Wang Xiangdong Zhu Xuening Chen Xiao Yang Kai Zhang Yujiang Fan Xingdong Zhang | 2018 | Regenerative Biomaterials2018，5，3： | 8 |
| 4 | Oral health in China: from vision to action显示文摘Chinese president Xi Jinping made clear at the National Health and Wellness Conference that health is the prerequisite for people's all-around development and a precondition for the sustainable development of China. Oral health is an indispensable component of overall health in humans. However, the long neglect of oral health in overall health agendas has made oral diseases an increasing concern. With this perspective, we described the global challenges of oral diseases, with an emphasis on the challenges faced by China. We also described and analyzed the recently released health policies of the Chinese government, which aim to guide midterm and long-term oral health promotion in China. More importantly, we called for specific actions to fulfill the larger goal of oral health for the nation. The implementation of primordial prevention efforts against oral diseases, the integration of oral health into the promotion of overall health, and the management of oral diseases in conjunction with other chronic non-communicable diseases with shared risk factors were highly recommended. In addition, we suggested the reform of standard clinical residency training, the development of domestic manufacturing of dental equipment and materials, the revitalization traditional Chinese medicine for the prevention and treatment of oral diseases, and integration of oral health promotion into the Belt and Road Initiative. We look forward to seeing a joint effort from all aspects of the society to fulfill the goal of Healthy China 2030 and ensure the oral health of the nation. | Xuedong Zhou Xin Xu Jiyao Li Deyu Hu Tao Hu Wei Yin Yujiang Fan Xingdong Zhang | 2018 | International Journal of Oral Science2018，10，1： | 7 |
| 5 | The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging:An in vivo study显示文摘The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field,such as collagen or hyaluronic acid,etc.However,the biosafety and efficacy of these implants are still challenged.In this study,a skin damage animal model was prepared by UV-photoaging and recombinant humanized type Ⅲ collagen(rhCol Ⅲ)was applied as a bioactive material to implant in vivo to study its biological effect,comparing with saline and uncrosslinked hyaluronic acid(HA).Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment.Histological observation,specific gene expression and other molecular biological methods were applied by the end of the animal experiment.The results indicated that rhCol Ⅲ could alleviate the skin photoaging caused by UV radiation,including reduce the thickening of epidermis and dermis,increase the secretion of Collagen Ⅰ(Col Ⅰ)and Collagen Ⅲ(Col Ⅲ)and remodel of extracellular matrix(ECM).Although the cell-material interaction and mechanism need more investigation,the effect of rhCol Ⅲ on damaged skin was discussed from influence on cells,reconstruction of ECM,and stimulus of small biological molecules based on current results.In conclusion,our findings provided rigorous biosafety information of rhCol Ⅲ and approved its potential in skin repair and regeneration.Although enormous efforts still need to be made to achieve successful translation from bench to clinic,the recombinant humanized collagen showed superiorities from both safety and efficacy aspects. | Jing Wang He Qiu Yang Xu Yongli Gao Peijie Tan Rui Zhao Zhanhong Liu Yajun Tang Xiangdong Zhu Chongyun Bao Hang Wang Hai Lin Xingdong Zhang | 2022 | Bioactive Materials2022，7，5： | 7 |
| 6 | The optimized preparation of HA/L-TiO2/D-TiO2 composite coating on porous titanium and its effect on the behavior osteoblasts显示文摘Various surface bioactivation technology has been confirmed to improve the osteogenic ability of porous titanium(pTi)implants effectively.In this study,a three-layered composite coating,i.e.outer layer of hydroxyapatite(HA),middle layer of loose titanium dioxide(L-TiO2)and inner layer of dense TiO2(D-TiO2),was fabricated on pTi by a combined processing procedure of pickling,alkali heat(AH),anodic oxidation(AO),electrochemical deposition(ED)and hydrothermal treatment(HT).After soaking in simulated body fluid for 48 h,the surface of the AHAOEDHT-treated pTi was completely covered by a homogeneous apatite layer.Using MC3T3-E1 pro-osteoblasts as cell model,the cell culture revealed that both the pTi without surface treatment and the AHAOEDHT sample could support the attachment,growth and proliferation of the cells.Compared to the pTi sample,the AHAOEDHT one induced higher expressions of osteogenesis-related genes in the cells,including alkaline phosphatase,Type I collagen,osteopontin,osteoclast inhibitor,osteocalcin and zinc finger structure transcription factor.As thus,besides the good corrosion resistance,the HA/L-TiO2/D-TiO2-coated pTi had good osteogenic activity,showing good potential in practical application for bone defect repair. | Xi Fu Xingyu Zhou Pin Liu Hewei Chen Zhanwen Xiao Bo Yuan Xiao Yang Xiangdong Zhu Kai Zhang Xingdong Zhang | 2020 | Regenerative Biomaterials2020，7，5： | 5 |
| 7 | Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor显示文摘T 房间 receptor-CD3 建筑群(TCR ) 是能基于 CD3 细胞质的领域的各种各样的生物化学的变化开始抗原特定的有免疫力的回答的一台万用的发信号机器,但是内在的结构的基础留下逃犯。这里,我们开发了生物物理的途径学习 CD3 细胞质的领域的 conformational 动力学(CD3 CD ) 。在单个分子的水平,我们发现那 CD3 CD 能与三个功能的主题的不同坦诚有多重 conformational 状态,即, ITAM, BRS 和 PRS。因为,这些符合构造被产生 CD3 CD 有异构的类脂化合物绑定性质因此有异构的动力学。实时房间的成像实验证明不同抗原刺激能稳定 CD3 在不同符合构造的 CD 。类脂化合物依赖者 conformational 动力学因此为 TCR 的万用的发信号性质提供结构的基础。 | Xingdong Guo Chengsong Yan Hua Li Wenmao Huan Xiaoshan Shi Min Huang Yingfang Wang Weiling Pan Mingjun Cai Lunyi Li Wei Wu Yibing Bai Chi Zhang Zhijun Liu Xinyan Wang Xiaohui F Zhang Chun Tang Hongda Wang Wanli Liu Bo Ouyang Catherine C Wong Yi Cao Chenqi Xu | 2017 | Cell Research2017，27，4： | 5 |
| 8 | 3D printing of calcium phosphate bioceramic with tailored biodegradation rate for skull bone tissue reconstruction显示文摘The bone regenerative scaffold with the tailored degradation rate matching with the growth rate of the new bone is essential for adolescent bone repair.To satisfy these requirement,we proposed bone tissue scaffolds with controlled degradation rate using osteoinductive materials(Ca-P bioceramics),which is expected to present a controllable biodegradation rate for patients who need bone regeneration.Physicochemical properties,porosity,compressive strength and degradation properties of the scaffolds were studied.3D printed Ca-P scaffold(3DS),gas foaming Ca-P scaffold(FS)and autogenous bone(AB)were used in vivo for personalized beagle skull defect repair.Histological results indicated that the 3DS was highly vascularized and well combined with surrounding tissues.FS showed obvious newly formed bone tissues.AB showed the best repair effect,but it was found that AB scaffolds were partially absorbed and degraded.This study indicated that the 3D printed Ca-P bioceramics with tailored biodegradation rate is a promising candidate for personalized skull bone tissue reconstruction. | Boqing Zhang Huan Sun Lina Wu Liang Ma Fei Xing Qingquan Kong Yujiang Fan Changchun Zhou Xingdong Zhang | 2019 | Bio-Design and Manufacturing2019，2，3： | 5 |
| 9 | BMSCs-assisted injectable Col I hydrogel-regenerated cartilage defect by reconstructing superficial and calcified cartilage显示文摘The self-healing capacity of cartilage was limited due to absence of vascular,nervous and lymphatic systems.Although many clinical treatments have been used in cartilage defect repair and shown a promising repair result in short term,however,regeneration of complete zonal structure with physiological function,reconstruction cartilage homeostasis and maintaining long-term repair was still an unbridgeable chasm.Cartilage has complex zonal structure and multiple physiological functions,especially,superficial and calcified cartilage played an important role in keeping homeostasis.To address this hurdle of regenerating superficial and calcified cartilage,injectable tissue-induced type I collagen(Col I)hydrogel-encapsulated BMSCs was chosen to repair cartilage damage.After 1 month implantation,the results demonstrated that Col I gel was able to induce BMSCs differentiation into chondrocytes,and formed hyaline-like cartilage and the superficial layer with lubrication function.After 3 months post-surgery,chondrocytes at the bottom of the cartilage layer would undergo hypertrophy and promote the regeneration of calcified cartilage.Six months later,a continuous anatomical tidemark and complete calcified interface were restored.The regeneration of neo-hyaline cartilage was similar with adjacent normal tissue on the thickness of the cartilage,matrix secretion,collagen type and arrangement.Complete multilayer zonal structure with physiological function remodeling indicated that BMSCs-assisted injectable Col I hydrogel could reconstruct cartilage homeostasis and maintain long-term therapeutic effect. | Hanxu Cai PeileiWang Yang Xu Ya Yao Jia Liu Tao Li Yong Sun Jie Liang Yujiang Fan Xingdong Zhang | 2020 | Regenerative Biomaterials2020，7，1： | 4 |
| 10 | Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo显示文摘Extracellular matrix(ECM)-based biomaterials are promising candidates in cartilage tissue engineering by simulating the native microenvironment to regulate the chondrogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)without exogenous growth factors.The biological properties of ECM scaffolds are primarily depended on the original source,which would directly influence the chondrogenic effects of the ECM materials.Despite the expanding investigations on ECM scaffolds in recent years,the selection of optimized ECM materials in cartilage regeneration was less reported.In this study,we harvested and compared the articular cartilage ECM from newborn,juvenile and adult rabbits.The results demonstrated the significant differences in the mechanical strength,sulphated glycosaminoglycan and collagen contents of the different aged ECM,before and after decellularization.Consequently,different compositional and mechanical properties were shown in the three ECM-based collagen hydrogels,which exerted age-dependent chondrogenic inducibility.In general,both in vitro and in vivo results suggested that the newborn ECM promoted the most chondrogenesis of BMSCs but led to severe matrix calcification.In contrast,BMSCs synthesized the lowest amount of cartilaginous matrix with minimal calcification with adult ECM.The juvenile ECM achieved the best overall results in promoting chondrogenesis of BMSCs and preventing matrix calcification.Together,this study provides important information to our current knowledge in the design of future ECM-based biomaterials towards a successful repair of articular cartilage. | Xiuyu Wang Yan Lu Wan Wang Qiguang Wang Jie Liang Yujiang Fan Xingdong Zhang | 2020 | Regenerative Biomaterials2020，7，6： | 4 |
| 11 | Modeling and Optimal Design of Planar Linkage Mechanism of Coupled Joint Clearances for Manufacturing显示文摘The uncertainty of the mechanism motion error is mostly caused by the manufacturing process,so the motion error cannot be effectively predicted at the design phase.The problems of manufacturing complexity and the relationship between design and manufacturing are analyzed,and the influence of dimensional tolerance and fit tolerance on the motion accuracy of the system is considered in the design process.Then based on the Monte Carlo simulation,an optimal design model of planar linkage mechanism is set up.A typical offset slider-crank mechanism is used as an illustrative example to carry out the optimal design.Compared with the result of typical robustness design,the similar variation characteristics of the mean value and the standard deviation can be found,so the proposed method is effective.The method is furthermore applied in the optimization of the schemes with different fit tolerances and the prediction of motion errors in the design phase is achieved.A set of quantitative evaluation system for mechanism optimal design is provided.Finally,a basic strategy is presented to balance the motion precision and manufacturing cost. | Yang Bo Wang Xingdong Kong Jianyi Zhang Hua | 2018 | Transactions of Nanjing University of Aeronautics and Astronautics2018，35，4： | 3 |
| 12 | Evaluation on the corrosion resistance, antibacterial property and osteogenic activity of biodegradable Mg-Ca and Mg-Ca-Zn-Ag alloys显示文摘The rapid degradation of magnesium(Mg)-based implants in physiological environment limits its clinical applications, and alloying treatment is an effective way to regulate the degradation rate of Mg-based materials. In the present study, three Mg alloys, including Mg-0.8Ca(denoted as ZQ), Mg-0.8Ca-5Zn-1.5Ag(denoted as ZQ71) and Mg-0.8Ca-5Zn-2.5Ag(denoted as ZQ63), were fabricated by alloying with calcium(Ca), zinc(Zn) and silver(Ag). The results obtained from electrochemical corrosion tests and in vitro degradation evaluation demonstrated that the three Mg alloys exhibited distinct corrosion resistance, and ZQ71 exhibited the lowest degradation rate in vitro among them. After addition of Zn and Ag, the antibacterial potential of Mg alloys was also enhanced. The in vitro cell experiments showed that all the three Mg alloys had good biocompatibility. After implantation in a rat femoral defect, ZQ71 showed significantly higher osteogenic activity and bone substitution rate than ZQ63 and ZQ, due to its higher corrosion resistance as well as the stimulatory effects of the released metallic ions. In addition, the average daily degradation rate of each Mg alloy in vivo was significantly higher than that in vitro, as could be due to the implantation site located in the highly vascularized trabecular region. Importantly, the correlations between the in vitro and in vivo degradation parameters of the Mg alloys were systematically analyzed to find out the potential predictors of the in vivo degradation performance of the materials. The current work not only evaluated the clinical potential of the three biodegradable Mg alloys as bone grafts but also provided a feasible approach for predicting the in vivo degradation behavior of biodegradable materials. | Hewei Chen Bo Yuan Rui Zhao Xiao Yang Zhanwen Xiao Antoniac Aurora Bita Ana Iulia Xiangdong Zhu Antoniac Vasile Iulian Xingdong Zhang | 2022 | Journal of Magnesium and Alloys2022，10，12： | 3 |
| 13 | Dynamic mechanical loading facilitated chondrogenic differentiation of rabbit BMSCs in collagen scaffolds显示文摘Mechanical signals have been played close attention to regulate chondrogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).In this study,dynamic mechanical loading simulation with natural frequencies and intensities were applied to the 3D cultured BMSCs–collagen scaffold constructs.We investigated the effects of dynamic mechanical loading on cell adhesion,uniform distribution,proliferation,secretion of extracellular matrix(ECM)and chondrogenic differentiation of BMSCs–collagen scaffold constructs.The results indicated that dynamic mechanical loading facilitated the BMSCs adhesion,uniform distribution,proliferation and secretion of ECM with a slight contraction,which significantly improved the mechanical strength of the BMSCs–collagen scaffold constructs for better mimicking the structure and function of a native cartilage.Gene expression results indicated that dynamic mechanical loading contributed to the chondrogenic differentiation of BMSCs with higher levels of AGG,COL2A1 and SOX9 genes,and prevented of hypertrophic process with lower levels of COL10A1,and reduced the possibility of fibrocartilage formation due to down-regulated COL1A2.In conclusion,this study emphasized the important role of dynamic mechanical loading on promoting BMSCs chondrogenic differentiation and maintaining the cartilage phenotype for in vitro reconstruction of tissue-engineered cartilage,which provided an attractive prospect and a feasibility strategy for cartilage repair. | Wanxu Cao Weimin Lin Hanxu Cai Yafang Chen Yi Man Jie Liang Qiguang Wang Yong Sun Yujiang Fan Xingdong Zhang | 2019 | Regenerative Biomaterials2019，6，2： | 3 |
| 14 | Preparation of bioactive titanium metal via anodic oxidation treatment显示文摘 | Bangcheng Yang Masaiki Uchida Hyun-Min Kim Xingdong Zhang Tadashi Kokubo | 2003 | Biomaterials2003，，6： | 3 |
| 15 | Optimal regenerative repair of large segmental bone defect in a goat model with osteoinductive calcium phosphate bioceramic implants显示文摘So far,how to achieve the optimal regenerative repair of large load-bearing bone defects using artificial bone grafts is a huge challenge in clinic.In this study,a strategy of combining osteoinductive biphasic calcium phosphate(BCP)bioceramic scaffolds with intramedullary nail fixation for creating stable osteogenic microenvironment was applied to repair large segmental bone defects(3.0 cm in length)in goat femur model.The material characterization results showed that the BCP scaffold had the initial compressive strength of over 2.0 MPa,and total porosity of 84%.The cell culture experiments demonstrated that the scaffold had the excellent ability to promote the proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells(BMSCs).The in vivo results showed that the intramedullary nail fixation maintained the initial stability and structural integrity of the implants at early stage,promoting the osteogenic process both guided and induced by the BCP scaffolds.At 9 months postoperatively,good integration between the implants and host bone was observed,and a large amount of newborn bones formed,accompanying with the degradation of the material.At 18 months postoperatively,almost the complete new bone substitution in the defect area was achieved.The maximum bending strength of the repaired bone defects reached to the 100% of normal femur at 18 months post-surgery.Our results demonstrated the good potential of osteoinductive BCP bioceramics in the regenerative repair of large load-bearing bone defects.The current study could provide an effective method to treat the clinical large segmental bone defects. | Wei Zhi Xiaohua Wang Dong Sun Taijun Chen Bo Yuan Xiangfeng Li Xuening Chen Jianxin Wang Zhao Xie Xiangdong Zhu Kai Zhang Xingdong Zhang | 2022 | Bioactive Materials2022，7，5： | 3 |
| 16 | Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles显示文摘While most bone defects can be repaired spontaneously,the healing process can be complicated due to insuf-ficient bone regeneration when osteoporosis occurs.Synthetic materials that intrinsically stimulate bone for-mation without inclusion of exogenous cells or growth factors represent a highly desirable alternative to current grafting strategies for the management of osteoporotic defects.Herein,we developed a series of hydroxyapatite bioceramics composed of a microwhiskered scaffold(wHA)reinforced with multiple layers of releasable hy-droxyapatite nanoparticles(nHA).These novel bioceramics(nwHA)are tunable to optimize the loading amount of nHA for osteoporotic bone formation.The utility of nwHA bioceramics for the proliferation or differentiation of osteoporotic osteoblasts in vitro is demonstrated.A much more compelling response is seen when bioceramics are implanted in critical-sized femur defects in osteoporotic rats,as nwHA bioceramics promote significantly higher bone regeneration and delay adjacent bone loss.Moreover,the nwHA bioceramics loaded with a mod-erate amount of nHA can induce new bone formation with a higher degree of ossification and homogenization.Two types of osteogenesis inside the nwHA bioceramic pores were discovered for the first time,depending on the direction of growth of the new bone.The current study recommends that these tailored hybrid micro/nano-structured bioceramics represent promising candidates for osteoporotic bone repair. | Rui Zhao Tieliang Shang Bo Yuan Xiangdong Zhu Xingdong Zhang Xiao Yang | 2022 | Bioactive Materials2022，7，11： | 2 |
| 17 | Denoising method of X-ray phase contrast DR image for TRISO-coated fuel particles显示文摘TRISO(tristructural-isotropic) fuel is a type of micro fuel particles used in high-temperature gas-cooled reactors(HTGRs).Among the quality evaluation methods for such particles,in-line phase contrast imaging technique(PCI) is more feasible for nondestructive measurement.Due to imaging hardware limitations,high noise level is a distinct feature of PCI images,and as a result,the dimensional measurement accuracy of TRISO-coated fuel particles decreases.Therefore,we propose an improved denoising hybrid model named as NL P-M model which introduces non-local theory and retains the merits of the Perona-Malik(P-M) model.The improved model is applied to numerical simulation and practical PCI images.Quantitative analysis proves that this new anisotropic diffusion model can preserve edge or texture information effectively,while ruling out noise and distinctly decreasing staircasing artifacts.Especially during the process of coating layer thickness measurement,the NL P-M model makes it easy to obtain continuous contours without noisy points or fake contour segments,thus enhancing the measurement accuracy.To address calculation complexity,a graphic processing unit(GPU) is adopted to realize the acceleration of the NL P-M denoising. | Min Yang Jianhai Zhang Fanyong Meng Sung-Jin Song Xingdong Li Wenli Liu Dongbo Wei | 2013 | Particuology2013，11，6： | 2 |
| 18 | Effects of soil stoichiometry of the CaCO_3/available phosphorus ratio on plant density in Artemisia ordosica communities显示文摘In soil, CaCO3 can reduce the availability of phosphorus in arid and semi-arid regions, thereby influencing plant growth. However, the effects of soil stoichiometry of the CaCO3/available phosphorus ratio (CaCO3/AP ratio) on plant density are unknown. In this study, we examined 4 Artemisia ordosica communities located in arid and semi-arid regions, and performed a pot experiment with an orthogonal design to examine the relationships between the soil CaCO3/AP ratio and plant density. The results showed that the soil stoichiometry of the CaCO3/AP ratio had a greater effect on growth of A. ordosica than either CaCO3 or AP as single factor. The influence of the soil CaCO3/AP ratio on density of A. ordosica was related to the climatic zone and the types and/or amplitudes of increases in the soil CaCO3/AP ratio. When the soil CaCO3/AP ratio was within the same amplitude and was less than 2.5, the A. ordosica density increased in the semi-arid region, whereas the density decreased with increasing soil CaCO3/AP ratios in the arid region. In the semi-arid region, when the soil CaCO3/AP ratio was within the same amplitude and the increase in the soil CaCO3 content was greater than the increase in soil AP, the A. ordosica density increased with increasing soil CaCO3/AP ratios. This means that the relationship between the soil CaCO3/AP ratio and the A. ordosica density remained the same. In the arid region, an increase in the soil CaCO3/AP ratio as a result of increased soil CaCO3 content and decreased soil AP resulted in increased density of A. ordosica. Therefore, the relationship between soil CaCO3/AP ratios and density of A. ordosica was altered by these changes in soil chemistry. Thus, the soil stoichiometry of the CaCO3/AP ratio is an important factor affecting the density of A. ordosica in arid and semi-arid regions. | ZHAO XueLai HE XingDong XUE PingPing ZHANG Ning WU Wei LI Rong CI HuaCong XU JingJing GAO YuBao ZHAO HaLin | 2012 | Chinese Science Bulletin2012，57，5： | 2 |
| 19 | Fabrication and biological evaluation of 3D-printed calcium phosphate ceramic scaffolds with distinct macroporous geometries through digital light processing technology显示文摘Digital light processing(DLP)-based 3D printing technique holds promise in fabricating scaffolds with high precision.Here raw calcium phosphate(CaP)powders were modified by 5.5%monoalcohol ethoxylate phosphate(MAEP)to ensure high solid loading and low viscosity.The rheological tests found that photocurable slurries composed of 50wt%modified CaP powders and 2wt%toners were suitable for DLP printing.Based on geometric models designed by computer-aided design(CAD)system,three printed CaP ceramics with distinct macroporous structures were prepared,including simple cube,octet-truss and inverse face-centered cube(fcc),which presented the similar phase composition and microstructure,but the different macropore geometries.Inverse fcc group showed the highest porosity and compressive strength.The in vitro and in vivo biological evaluations were performed to compare the bioactivity of three printed CaP ceramics,and the traditional foamed ceramic was used as control.It suggested that all CaP ceramics exhibited good biocompatibility,as evidence by an even bone-like apatite layer formation on the surface,and the good cell proliferation and spreading.A mouse intramuscular implantation model found that all of CaP ceramics could induce ectopic bone formation,and foam group had the strongest osteoinduction,followed by inverse fcc,while cube and octet-truss had the weakest one.It indicated that macropore geometry was of great importance to affect the osteoinductivity of scaffolds,and spherical,concave macropores facilitated osteogenesis.These findings provide a strategy to design and fabricate high-performance orthopedic grafts with proper pore geometry and desired biological performance via DLP-based 3D printing technique. | Jing Wang Yitao Tang Quanle Cao Yonghao Wu Yitian Wang Bo Yuan Xiangfeng Li Yong Zhou Xuening Chen Xiangdong Zhu Chongqi Tu Xingdong Zhang | 2022 | Regenerative Biomaterials2022，9，1： | 2 |
| 20 | The HuaBiao project:whole-exome sequencing of 5000 Han Chinese individuals显示文摘Next-generation sequencing technologies have significantly accelerated the identification of disease-causing mutations and facilitated the emergence of personalized medicine(Genomes Project Consortium et al.,2015;Goodwin et al.,2016;Sirugo et al.,2019).In comparison with whole-genome sequencing,whole-exome sequencing(WES),which covers the coding regions of the genome,offers a cost-efficacy balance.WES provides deeper sequencing depth(>100)and allows the more accurate detection of rare variants that are tailored for clinical applications(Lek et al.,2016). | Meng Hao Weilin Pu Yi Li Shaoqing Wen Chang Sun Yanyun Ma Hongxiang Zheng Xingdong Chen Jingze Tan Guoqing Zhang Menghan Zhang Shuhua Xu Yi Wang Hui Li Jiucun Wang Li Jin | 2021 | Journal of Genetics and Genomics2021，48，11： | 2 |
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