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| 1 | Pathogen Spectrum of Hand,Foot,and Mouth Disease Based on Laboratory Surveillance—China,2018显示文摘What is already known about this topic?Enterovirus 71(EV-A71)is the main causative pathogen for severe and fatal patients with Hand,Foot,and Mouth Disease(HFMD)in China's Mainland from 2008 to 2017.Non-EV-A71 and non-CV-A16(other enterovirus)serotypes were the major causativeserotypes for mild HFMD in years of 2013,2015,and 2017.What is added by this report?In 2018,other enterovirus serotypes replaced EV-A71 for the first time as the major cause of severe HFMD with a proportion of 70.7%.However,at the national level,only a small proportion of the other enterovirus serotypes were further identified as CV-A6 and CVA10.What are the limitations for public health practice?Further identification of other enterovirus serotypes is highly recommended for provincial CDCs,especially for severe HFMD.Studies contributing to a multivalent vaccine for HFMD should be prioritized. | Fengfeng Liu Minrui Ren Shumin Chen Taoran Nie Jinzhao Cui Lu Ran Zhongjie Li Zhaorui Chang | 2020 | China CDC weekly2020，2，11： | 8 |
| 2 | Production of transgenic mice carrying green fluorescence protein gene by a lentiviral vector-mediated approach显示文摘pseudo-lentivirus,带表示盒子的绿荧光蛋白质(GFP ) ,被注入仙子卵黄在移植进收养的母亲的输卵管前的鼠科的使肥沃的卵母细胞的空格。GFP 转基因的小狗然后被获得。由 PCR 扩大,荧光灯的显微镜学和流动帮助了排序分析的 cytometry,我们发现 transgene 的集成率在上面被估计 40% 。实时 PCR 分析显示综合 GFP 盒子的拷贝数字在 40 附近。在 situ 杂交分析荧光灯证明集成模式随机却可继承。有多集成地点和各种各样的表示层次的转基因的老鼠象研究一样在实践拥有了大价值。此处报导的途径提供一个有效方法给产生并且屏蔽转基因的老鼠紧张的有效方法。 | ZHANG Jingzhi GUO Xinbing XIE Shuyang ZHU Yiwen HUANG Ying WANG Shu REN Zhaorui | 2006 | Progress in Natural Science:Materials International2006，16，8： | 6 |
| 3 | High expression of human serum albumin in milk of trans-genie mice directed by the goat β-casein gene promoter region显示文摘We have constructed a mammary gland expression vector that contained the goat β-casein gene pro-moter, 5’upstream regulatory region, exons 1, 2, intron 1 as well as the human serum albumin (hALB) mini-gene (including the full-long sequences of hALB cDNA and its intron 1). Injection of the vector into mouse tail veins showed that the recombinant construct was expressed only in mammary glands. The vector was microinjected into the mouse fertilized eggs, followed by transferring the eggs into the foster mice. 33 F0 mice were obtained. Of the 33, 8 mice (5 , 3 ) were transgenic with hALB gene integration identified by PCR as well as Southern blot hybridization. The integration rate was 24.2% (8/33). Western blot analysis showed that 3 female transgenic mice had hALB expression in their milk. The hALB contents in milk reached 3.54, 0.21 and 3.03 g/L, respectively. | HUANG Ying, HUANG Ying, HUANG Zan,YAN Jingbin, MA Zhanlu, SHENG Min,REN Zhaorui, ZENG Yitao & HUANG ShuzhenShanghai Institute of Medical Genetics, Shanghai Children’s Hospital, Shanghai 200040, China | 2001 | Chinese Science Bulletin2001，46，7： | 3 |
| 4 | The Mesenchymal Stem Cells Derived from Transgenic Mice Carrying Human Coagulation Factor Ⅷ Can Correct Phenotype in Hemophilia A Mice显示文摘Hemophilia A(HA)is an inherited X-linked recessive bleeding disorder caused by coagulant factor Ⅷ(FⅧ)deficiency.Previous studies showed that introduction of mesenchymal stem cells(MSCs)modified by FⅧ-expressing retrovirus may result in phenotypic correction of HA animals.This study aimed at the investigation of an alternative gene therapy strategy that may lead to sustained FⅧ transgene expression in HA mice.B-domain-deleted human FⅧ(hFⅧBD)vector was microinjected into single-cell embryos of wild-type mice to generate a transgenic mouse line,from which hFⅧBD-MSCs were isolated,followed by transplantation into HA mice.RT-PCR and real-time PCR analysis demonstrated the expression of hFⅧBD in multi-organs of recipient HA mice.Immunohistochemistry showed the presence of hFⅧBD positive staining in multi-organs of recipient HA mice.ELISA indicated that plasma hFⅧBD level in recipient mice reached its peak(77 ng/mL)at the 3rd week after implantation,and achieved sustained expression during the 5-week observation period.Plasma FⅧ activities of recipient HA mice increased from 0%to 32%after hFⅧBD-MSCs transplantation.APTT(activated partial thromboplastin time)value decreased in hFⅧBD-MSCs transplanted HA mice compared with untreated HA mice(45.5 s vs.91.3 s).Our study demonstrated an effective phenotypic correction in HA mice using genetically modified MSCs from hFⅧBD transgenic mice. | Qing Wang Xiuli Gong Zhijuan Gong Xiaoyie Ren Zhaorui Ren Shuzhen Huang Yitao Zeng | 2013 | Journal of Genetics and Genomics2013，40，12： | 2 |
| 5 | Expression of biologically active human clotting factor Ⅸ(hFⅨ) in the mammary gland of transgenic mice显示文摘The DNA of human factor Ⅸ (hFⅨ) gene vector pMCⅨm, which had been proven to be able to express in in vitro and living cells, was introduced into 586 zygotes of Kunming White Mice by positive pressure microinjection technique with manual operation. The 499 survival embryos after microinjection were then transferred into pseudopregnant recipient mice and 216 F 0 pups were born. The analysis of PCR and Southern blot hybridization showed that, of the 216, 6 (2 females and 4 males) were integrated with foreign DNA in their genomes, giving an integration frequency of 3% (6/216). Two F\-0 female transgenic mice could express hFⅨ protein in their milk and the content was over 100 ng/mL as measured with ELISA. The biological activities of hFⅨ in the milk of two F\-0 mice were 44 67% and 79 43%, respectively. | HUANG Ying 1, ZHANG Kezhong 2, HUANG Wenying 1, LU Daru 2, HUANG Ying 1, MA Zhanlu 1, REN Zhaorui 1, QIU Xinfang 2, XUE Jinglun 2, ZENG Yitao 1 and HUANG Shuzhen 1* 1. Shanghai Institute of Medical Genetics, Shanghai Children’s Hospita | 1998 | Chinese Science Bulletin1998，43，15： | 2 |
| 6 | Amelioration of β^(654)-thalassemia in mouse model with the knockdown of aberrantly spliced β-globin mRNA显示文摘Large amounts of aberrantly spliced mRNA from the β654 allele was present in erythroid cells, which might impair the erythropoiesis. A therapeutic strategy for β-thalassemia was explored by knocking down the aberrantly spliced mRNA of β-globin. Lentiviral vector with siRNA fragment targets on the specific portion of β654-globin aberrantly spliced pre-mRNA was constructed. In HeLa β654 cells, the siRNA vector could reduce approximately 60% of aberrantly spliced mRNA, which was assessed by RT-PCR and qRT-PCR. Furthermore, a disease model of β654 thalassemia mice with lentiviral-mediated siRNA was produced by subzonal injection (named Hβi-Hbbth-4/Hbb+ transgenic mice). Our results showed that the hemotological parameters were improved in Hβi-Hbbth-4/Hbb+ transgenic mice. This study provides a potential way for β654-thalassemia therapy by knocking down the aberrantly spliced β-globin mRNA, whilst supporting that the aberrantly spliced β-globin mRNA may aggravate the disease. | Shuyang Xie Wei Li Zhaorui Ren Jingzhi Zhang Xinbin Guo Shu Wang Shuzhen Huang Fanyi Zeng Yi-Tao Zeng | 2008 | Journal of Genetics and Genomics2008，35，10： | 1 |
| 7 | Molecular Analysis of the Huntington's Disease with Expanded CAG Trinucleotide Repeat in Chinese显示文摘The polymorphic CAG repeats in the IT15 gene in Chinese normal and Huntington’s dis-ease(HD)chromosomes were determined by using nested PCR and denaturing polyacry-lamide gel electrophoretic autoradiography as well as direct sequencing analysis.A total of40 normal individuals and 122 members of 13 unrelated HD families originating from Shang-hai,Jiangsu,Zhejiang,Anhui,Shandong,Guangdong and Henan,respectively,were in-volved in this study.The results showed that the(CAG)n repeat numbers in 270 normal al-leles ranged from 13 to 26 but most in 16;while in 54 HD alleles,the CAG repeats from 40to 94,with an unstable inheritance of expanded repeats in some families.There was no over-lap between the normal and affected alleles.Additionally,the presymptomatic diagnosis in103 family members at risk for HD disclosed that 35 individuals had HD alleles,which were-in accordance with the pedigree analysis and clinical investigation.All these results indicatedthat the dynamic mutation in IT15 gene was responsible for the genetic defect in the ChineseHD patients and that a correlation existed between the numbers of(CAG)n repeat and theonset age of the disease.All-of these provide valuable data for HD molecular diagnosis,ge-netic counselling and genetic health. | Zeng Yitao Mao Yuehua Chen Meijue Ren Zhaorui Zhou Gang Huang Shuzhen (Shanghai Institute of Medical Genetics,Shanghai Children’s Hospital,Shanghai 200040,P.R.China) Wang Xiuying~① Yie Wenghu~② Zhao Xiangzhi~③ (①Xuzhou Medical College,Xuzhou,JiangSu,P.R.China) (②Anhui Medical University,Aanhui,P.R.China) (③Henan Psychiatrical Institute,Zhengzhou,P.R.China) | 1995 | High Technology Letters1995，1，1： | 0 |
| 8 | Protective mechanism of quercetin in alleviating sepsis-related acute respiratory distress syndrome based on network pharmacology and in vitro experiments显示文摘BACKGROUND:Sepsis-related acute respiratory distress syndrome(ARDS)has a high mortality rate,and no effective treatment is available currently.Quercetin is a natural plant product with many pharmacological activities,such as antioxidative,anti-apoptotic,and anti-inflammatory effects.This study aimed to elucidate the protective mechanism of quercetin against sepsis-related ARDS.METHODS:In this study,network pharmacology and in vitro experiments were used to investigate the underlying mechanisms of quercetin against sepsis-related ARDS.Core targets and signaling pathways of quercetin against sepsis-related ARDS were screened and were verified by in vitro experiments.RESULTS:A total of 4,230 targets of quercetin,360 disease targets of sepsis-related ARDS,and 211 intersection targets were obtained via database screening.Among the 211 intersection targets,interleukin-6(IL-6),tumor necrosis factor(TNF),albumin(ALB),AKT serine/threonine kinase 1(AKT1),and interleukin-1β(IL-1β)were identified as the core targets.A Gene Ontology(GO)enrichment analysis revealed 894 genes involved in the inflammatory response,apoptosis regulation,and response to hypoxia.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis identified 106 pathways.After eliminating and generalizing,the hypoxia-inducible factor-1(HIF-1),TNF,nuclear factor-κB(NF-κB),and nucleotide-binding and oligomerization domain(NOD)-like receptor signaling pathways were identified.Molecular docking revealed that quercetin had good binding activity with the core targets.Moreover,quercetin blocked the HIF-1,TNF,NF-κB,and NODlike receptor signaling pathways in lipopolysaccharide(LPS)-induced murine alveolar macrophage(MH-S)cells.It also suppressed the inflammatory response,oxidative reactions,and cell apoptosis.CONCLUSION:Quercetin ameliorates sepsis-related ARDS by binding to its core targets and blocking the HIF-1,TNF,NF-κB,and NOD-like receptor signaling pathways to reduce inflammation,cell apoptosis,and oxidative stress. | Weichao Ding Wei Zhang Juan Chen Mengmeng Wang Yi Ren Jing Feng Xiaoqin Han Xiaohang Ji Shinan Nie Zhaorui Sun | 2024 | World Journal of Emergency Medicine2024，15，2： | 0 |
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