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| 1 | BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules显示文摘Error-free mitosis depends on accurate chromosome attachment to spindle microtubules, powered congression of those chromosomes, their segregation in anaphase, and assembly of a spindle midzone at mitotic exit. The centromere-associated kinesin motor CENP-E, whose binding partner is BubR1, has been implicated in congression of misaligned chromosomes and the transition from lateral kinetochore-microtubule association to end-on capture. Although previously proposed to be a pseudokinase, here we report the structure of the kinase domain of Drosophila melanogaster BubR1, revealing its folding into a conformation predicted to be catalytically active. BubR1 is shown to be a bona fide kinase whose phosphorylation of CENP-E switches it from a laterally attached microtubule motor to a plus-end microtubule tip tracker. Computational modeling is used to identify bubristatin as a selective BubR1 kinase antagonist that targets the αN1 helix of N-terminal extension and αC helix of the BubR1 kinase domain. Inhibition of CENP-E phosphorylation is shown to prevent proper microtubule capture at kinetochores and, surprisingly, proper assembly of the central spindle at mitotic exit. Thus, BubR1-mediated CENP-E phosphorylation produces a temporal switch that enables transition from lateral to end-on microtubule capture and organization of microtubules into stable midzone arrays. | Yuejia Huang Lin Lin Xing Liu Sheng Ye Phil Y. Yao Wenwen Wang Fengrui Yang Xinjiao Gao Junying Li Yin Zhang Jiancun Zhang Zhihong Yang Xu Liu Zhenye Yang Jianye Zang Maikun Teng Zhiyong Wang Ke Ruan Xia Ding Lin Li Don W. Cleveland Rongguang Zhang Xuebiao Yao | 2019 | Cell Research2019，29，7： | 8 |
| 2 | Engineering Ruthenium-Based Electrocatalysts for Effective Hydrogen Evolution Reaction显示文摘The investigation of highly effective,durable,and cost-effective electrocatalysts for the hydrogen evolution reaction(HER)is a prerequisite for the upcoming hydrogen energy society.To establish a new hydrogen energy system and gradually replace the traditional fossil-based energy,electrochemical water-splitting is considered the most promising,environmentally friendly,and efficient way to produce pure hydrogen.Compared with the commonly used platinum(Pt)-based catalysts,ruthenium(Ru)is expected to be a good alternative because of its similar hydrogen bonding energy,lower water decomposition barrier,and considerably lower price.Analyzing and revealing the HER mechanisms,as well as identifying a rational design of Ru-based HER catalysts with desirable activity and stability is indispensable.In this review,the research progress on HER electrocatalysts and the relevant describing parameters for HER performance are briefly introduced.Moreover,four major strategies to improve the performance of Ru-based electrocatalysts,including electronic effect modulation,support engineering,structure design,and maximum utilization(single atom)are discussed.Finally,the challenges,solutions and prospects are highlighted to prompt the practical applications of Rubased electrocatalysts for HER. | Yingjie Yang Yanhui Yu Jing Li Qingrong Chen Yanlian Du Peng Rao Ruisong Li Chunman Jia Zhenye Kang Peilin Deng Yijun Shen Xinlong Tian | 2021 | Nano-Micro Letters2021，13，10： | 5 |
| 3 | Allelopathic Effects of Eichhornia crassipes on the Growth of Microcystis aeruginosa显示文摘 | Xiaoxia Wu Zhenye Zhang Dingli Chen Junsong Zhang Wenbing Yang Yingen Jin | 2012 | Journal of Agricultural Science and Technology(A)2012，2，12： | 2 |
| 4 | Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance显示文摘Gβ γheterodimer 是一个重要信号变换器。Gβ然而,由于它为 Gγ 的要求对错误褶层敏感;并且为合适的合拢的女伴。房间怎么错误褶层 Gβ 处理;(mfGβ) 不是清楚的。这里,我们显示出那 mfGβ能是 polyubiquitinated 并且随后由 proteasome 降级。它与 MG132 在 proteasome 活动的抑制以后在 aggresomes 被扣押。Gβ 的持续激活; γ进一步发信号提高了 ubiquitinated Gβ 的细胞的层次;。而且, Nudel,细胞质的 dynein 的一个管理者,减指导结束的马达的微导管,直接与 unubiquitinated 和 ubiquitinated mfGβ 交往了;。增加两 mfGβ 的层次;并且 Nudel 支持了 Gβ 的协会;与 Nudel 和 dynein,以一种 dynein 依赖的方式导致柔韧的 aggresome 形成。由 RNAi 的 Nudel 的弄空减少了联系 dynein 的 mfGβ,损害了导致 MG132 的 aggresome 形成,并且显著地延长了初生的 Gβ 的半衰期;。因此, cytosolic mfGβ被 Nudel 招募到 dynein 并且为快速的隐遁和降级搬运了到中心体。如此的一个过程不仅消除 mfGβ高效地为蛋白质质量的控制,但是可以也帮助终止 Gβ γ发信号。 | Yihan Wan Zhenye Yang Jing Guo Qiangge Zhang Liyong Zeng Wei Song Yue Xiao Xueliang Zhu | 2012 | Cell Research2012，22，7： | 1 |
| 5 | Microstructures and Mechanical Properties of a New Multi-functional 460 MPa Grade Construction Structural Steel显示文摘This article reports a new generation of Q460 multi-functional construction structural steel,which has high strength(yield strength larger than 460 MPa),excellent toughness(higher than 110 J/cm^(2) at-60 ℃),lower yield ratio(lower than 0.8),good fire resistance(yield strength at 600 ℃ larger than two-thirds of its room-temperature yield strength)and better corrosion resistance.The eff ects of finish cooling temperature(FCT)on the microstructure and properties were studied by scanning electron microscopy(SEM),transmission electron microscopy(TEM),emission electron probe micro-analysis(EPMA),electron backscattering diff raction(EBSD),tensile tester,impact tester,periodic immersion cycle acceleration test and electrochemical experiment.The results show that the strength and toughness are simultaneously improved by decreasing the FCT due to more lath-like bainite with large number of dislocations,refined martensite/austenite(M/A)with higher carbon concentration and increased high angle boundaries.In addition,the fire resistance of the newly developed Q460 steel is obviously better than the conventional one,which is mainly due to non-recrystallized lath-like bainite with high dislocation density at elevated temperature.The corrosion resistance of the new Q460 steel is also improved due to the addition of Cu and Cr. | Zhenye Chen Zhangguo Lin Jianjun Qi Yang Feng Liqing Chen Guodong Wang | 2022 | Acta Metallurgica Sinica(English Letters)2022，35，7： | 1 |
| 6 | Towards Realizing Sign Language-to-Speech Conversion by Combining Deep Learning and Statistical Parametric Speech Synthesis显示文摘This paper realizes a sign language-to-speech conversion system to solve the communication problem between healthy people and speech disorders. 30 kinds of different static sign languages are firstly recognized by combining the support vector machine (SVM) with a restricted Boltzmann machine (RBM) based regulation and a feedback fine-tuning of the deep model. The text of sign language is then obtained from the recognition results. A context-dependent label is generated from the recognized text of sign language by a text analyzer. Meanwhile,a hiddenMarkov model (HMM) basedMandarin-Tibetan bilingual speech synthesis system is developed by using speaker adaptive training.The Mandarin speech or Tibetan speech is then naturally synthesized by using context-dependent label generated from the recognized sign language. Tests show that the static sign language recognition rate of the designed system achieves 93.6%. Subjective evaluation demonstrates that synthesized speech can get 4.0 of the mean opinion score (MOS). | Xiaochun An Hongwu Yang Zhenye Gan | 2016 | 国际计算机前沿大会会议论文集2016，，1： | 0 |
| 7 | Towards Realizing Mandarin-Tibetan Bi-lingual Emotional Speech Synthesis with Mandarin Emotional Training Corpus显示文摘This paper presents a method of hidden Markov model (HMM)-based Mandarin-Tibetan bi-lingual emotional speech synthesis by speaker adaptive training with a Mandarin emotional speech corpus.A one-speaker Tibetan neutral speech corpus, a multi-speaker Mandarin neutral speech corpus and a multi-speaker Mandarin emotional speech corpus are firstly employed to train a set of mixed language average acoustic models of target emotion by using speaker adaptive training.Then a one-speaker Mandarin neutral speech corpus or a one-speaker Tibetan neutral speech corpus is adopted to obtain a set of speaker dependent acoustic models of target emotion by using the speaker adap-tation transformation. The Mandarin emotional speech or the Tibetan emotional speech is finally synthesized from Mandarin speaker depen-dent acoustic models of target emotion or Tibetan speaker dependent acoustic models of target emotion. Subjective tests show that the aver-age emotional mean opinion score is 4.14 for Tibetan and 4.26 for Mandarin. The average mean opinion score is 4.16 for Tibetan and 4.28 for Mandarin. The average degradation opinion score is 4.28 for Tibetan and 4.24 for Mandarin. Therefore, the proposed method can synthesize both Tibetan speech and Mandarin speech with high naturalness and emotional expression by using only Mandarin emotional training speech corpus. | Peiwen Wu Hongwu Yang Zhenye Gan | 2017 | 国际计算机前沿大会会议论文集2017，，2： | 0 |
| 8 | Application of a Nanostructured Composite Material Constructed by Self-Assembly of Titanoniobate Nanosheets and Cobalt Porphyrin to Electrocatalytic Reduction of Oxygen显示文摘 | Jinpeng Li Xiaobo Zhang Binbin Pan Jiasheng Xu Lin Liu Juanjuan Ma Min Yang Zhenye Zhang Zhiwei Tong | 2016 | Chinese Journal of Chemistry2016，34，10： | 0 |
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