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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | BML - 111, a lipoxin receptor agonist, protected carbon tetrachloride - induced hepatic fibrosis in rats显示文摘 | Zhou XY Yu ZJ Yan D | 2013 | Inflammation2013，36，5： | 1 |
| 3 | Evaluation of pulmonary em- bolism in pediatric patients with nephrotic syndrome with dual en- ergy CT pulmonary angiography 显示文摘 | Zhang LJ Wang ZJ Zhou CS | 2012 | Acad Radiol2012，19，3： | 1 |
| 4 | A prognostic model to pre- dict survival in stage ]]I colon cancer patients based on histological grade, preoperative carcinoembryonic antigen level and the neutro-phil lymphocyte ratio 显示文摘 | Wuxiao ZJ Zhou HY Wang KF | 2015 | Asian Pac J Cancer Prey2015，16，2： | 1 |
| 5 | Highly viru porcine reproductive and respiratory syndrome virus emerged in China显示文摘 | Zhou lent YJ Hao XF Tian ZJ | 2008 | Trans- bound Emerg Dis2008，55，34： | 1 |
| 6 | Securing Ad Hoe networks显示文摘 | Zhou LD Hass zj | 1999 | IEEE Network1999，13，6： | 1 |
| 7 | Impact of clopidogrel resistance on thrombotic events after percutaneous coronary intervention wiht drug-eluting stent显示文摘 | Wang ZJ Zhou YJ Liu YY | 2009 | Thumb Res2009，124，1： | 1 |
| 8 | Nutrient and energy requirements of red panda (Ailurus fulgens) during lactation显示文摘 | ZJ Feng ZW Wang A Zhou JC Hu | 1999 | Mammalia1999，63，1： | 1 |
| 9 | Seasonal energy requirements of red panda (Ailurus fulgens)显示文摘 | ZW Wang ZJ Feng M Li A Zhou | 2000 | Zoo Biology2000，19，： | 1 |
| 10 | Interleukin 22-producing CD4+ T cells in malignant pleural effusion显示文摘 | Ye ZJ Zhou Q Yin W | 2012 | Cancer Lett2012，326，1： | 1 |
| 11 | Ribosomal-sequence-directed selection for endophytic streptomyces strains antagonistic to Ralstonia solanacearum to control tomato bacterial wilt显示文摘 | TAN HM ZHOU SN DENG ZJ | 2011 | Biological Control2011，59，2： | 1 |
| 12 | Molecular epidemiology of den- gue viruses in southern China from 1978 to 2006 显示文摘 | Wu WL Bai ZJ zhou HQ | 2011 | Virol J2011，，8： | 1 |
| 13 | Impaired angiogenesis, delayed wound healing and retarded tumor growth in perlecan heparan sulfatedeficient ,nice 显示文摘 | Zhou ZJ Wang JM Cao RH | 2004 | Cancer Res2004，64，14： | 1 |
| 14 | Diagnostic value of N-terminalpro-brain natriuretic peptide for pleural effusion due toheart failure:a meta-analysis显示文摘 | Zhou Q Ye ZJ Su Y | 2010 | Heart2010，96，15： | 1 |
| 15 | Securing Ad Hoc Networks显示文摘 | ZHOU L HAAS ZJ | 1999 | IEEE Networks1999，13，6： | 1 |
| 16 | Effect and possible mech- anism of muscle-splitting approach on muhifidus muscle injury and atrophy after posterior lumbar spine surgery显示文摘 | Hu ZJ Fang XQ Zhou ZJ | 2013 | J Bone Joint Surg Am2013，95，24： | 1 |
| 17 | Comparison of different antithrom- bntic regimens for patients with atrial fibrillation undergoing drug-elu- ting stent implantation显示文摘 | Gao F Zhou YJ Wang ZJ | 2010 | Circ J2010，74，： | 1 |
| 18 | Differentiation and immune regulation of IL-9-producing CIM+T cells in malignant pleural effusion显示文摘 | Ye ZJ Zhou Q Yin W | 2012 | Am J Respir Crit Care Med2012，186，11： | 1 |
| 19 | Draft genome of the kiwifruit Actinidia chinensis 显示文摘 | Huang SX Ding J Deng DJ Tang W Sun HH Liu DY Zhang L Niu XL Zhang X Meng M Yu JD Liu J Han Y Shi W Zhang DF Cao SQ Wei ZJ Cui YL Xia YH Zeng HP Bao K Lin L Min Y Zhang H Miao M Tang XF Zhu YZ Sui Y Li GW Sun HJ Yue JY Sun JQ Liu FF Zhou LQ Lei L Zheng XQ Liu M Huang L Song J Xu CH Li JW Ye KY Zhong SL Lu BR He GH Xiao FM Wang HL Zheng HK Fei ZJ Liu YS | 2013 | Nat Commun2013，4，： | 1 |
| 20 | Impact of clopidogrelresistance on thrombotic events after percutaneous coro-nary intervention with drugeluting stent显示文摘 | Wang ZJ Zhou YJ LiuYY | 2009 | ThrombRes2009，124，1： | 1 |
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