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| 1 | Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials显示文摘Bacillus subtilis is a commonly used commercial specie with broad applications in the fields of bioengineering and biotechnology.B.subtilis is capable of producing both biofilms and spores.Biofilms are matrix-encased multicellular communities that comprise various components including exopolysaccharides,proteins,extracellular DNA,and poly-γ-glutamic acid.These biofilms resist environmental conditions such as oxidative stress and hence have applications in bioremediation technologies.Furthermore,biofilms and spores can be engineered through biotechnological techniques for environmentally-friendly and safe production of bio-products such as enzymes.The ability to withstand with harsh conditions and producing spores makes Bacillus a suitable candidate for surface display technology.In recent years,the spores of such specie are widely used as it is generally regarded as safe to use.Advances in synthetic biology have enabled the reprogramming of biofilms to improve their functions and enhance the production of value-added products.Globally,there is increased interest in the production of engineered biosensors,biocatalysts,and biomaterials.The elastic modulus and gel properties of B.subtilis biofilms have been utilized to develop living materials.This review outlines the formation of B.subtilis biofilms and spores.Biotechnological engineering processes and their increasing application in bioremediation and biocatalysis,as well as the future directions of B.subtilis biofilm engineering,are discussed.Furthermore,the ability of B.subtilis biofilms and spores to fabricate functional living materials with self-regenerating,self-regulating and environmentally responsive characteristics has been summarized.This review aims to resume advances in biological engineering of B.subtilis biofilms and spores and their applications. | Muhammad Zubair Mohsin Rabia Omer Jiaofang Huang Ali Mohsin Meijin Guo Jiangchao Qian Yingping Zhuang | 2021 | Synthetic and Systems Biotechnology2021，6，3： | 2 |
| 2 | Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401 显示文摘 | XU Guoqian CHU Ju ZHUANG Yingping | 2008 | Biochemical Engineering Journal2008，38，2： | 1 |
| 3 | Understanding the scale-up of fermentation processes from the viewpoint of the flow field in bioreactors and the physiological response of strains显示文摘The production capability of a fermentation process is predominately determined by individual strains,which ultimately affected ultimately by interactions between the scale-dependent flow field developed within bioreactors and the physiological response of these strains.Interpreting these complicated interactions is key for better understanding the scale-up of the fermentation process.We review these two aspects and address progress in strategies for scaling up fermentation processes.A perspective on how to incorporate the multiomics big data into the scale-up strategy is presented to improve the design and operation of industrial fermentation processes. | Jianye Xia Guan Wang Meng Fan Min Chen Zeyu Wang Yingping Zhuang | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 1 |
| 4 | Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding 显示文摘 | Li Kuntai Liu Donghong Zhuang Yingping | 2008 | Biore Teeh2008，99，17： | 1 |
| 5 | Medium optimization for meilingmycin production by Streptomyces nanchangensis using response surface methodology显示文摘 | ZHUANG Yingping CHEN Bin CHU Ju | 2006 | Process Biochemistry2006，41，2： | 1 |
| 6 | Influence of Zn^2+ , Co^2+ and dimethylbenzimidazole on vitamin B12 biosynthesis by Pseudomonas denitrificans 显示文摘 | Li Kuntai Liu Donghong Zhuang Yingping etal | 2008 | World J Microbiol Biotechnol2008，24，： | 1 |
| 7 | Study on Industrial Fermentation Process on Multi-Levels and Its Optimization显示文摘 | Ju chu Yingping Zhuang | 2004 | Adv Biochem Engineer Biotechnology2004，87，： | 1 |
| 8 | Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401 显示文摘 | Xu Guoqian Chu Ju Zhuang Yingping | 2008 | Biochem Eng J2008，38，2： | 1 |
| 9 | High cell density and high expression of recombinant human ApoA-I_(Milano) in Escherichia coli by twice temperature-shifted induction显示文摘The effect of temperature on the formation of recombinant protein,apolipoprotein A-IMilano was investigated in the present study.The temperature of the initial growth phase was set at 30ºC,while temperature variation in induction phase was arranged in three modes.High cell-density culture of Escherichia coli and high expression of recombinant human by twice temperature-shifted induction were carried out.Experimental results showed that ApoA-IMilano reached 4.8 g/L with the final cell density of OD600,150.It was found that twice temperature-shifted induction could successfully avoid the effect of acetic acid on cell density and the expression of the product.The present study provides a basic procedure for the production of recombinant ApoA-IMilano. | ZHUANG Yingping MA Wenfeng GUO Meijin DING Mansheng CHU Ju ZHANG Siliang | 2006 | Frontiers in Biology2006，1，4： | 0 |
| 10 | Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry显示文摘Microbial cell factories(bacteria and fungi)are the leading producers of beneficial natural products such as lycopene,carotene,herbal medicine,and biodiesel etc.These microorganisms are considered efficient due to their effective bioprocessing strategy(monoculture-and consortial-based approach)under distinct processing conditions.Meanwhile,the advancement in genetic and process optimization techniques leads to enhanced biosynthesis of natural products that are known functional ingredients with numerous applications in the food,cosmetic and medical industries.Natural consortia and monoculture thrive in nature in a small proportion,such as wastewater,food products,and soils.In similitude to natural consortia,it is possible to engineer artificial microbial consortia and program their behaviours via synthetic biology tools.Therefore,this review summarizes the optimization of genetic and physicochemical parameters of the microbial system for improved production of natural products.Also,this review presents a brief history of natural consortium and describes the functional properties of monocultures.This review focuses on synthetic biology tools that enable new approaches to design synthetic consortia;and highlights the syntropic interactions that determine the performance and stability of synthetic consortia.In particular,the effect of processing conditions and advanced genetic techniques to improve the productibility of both monoculture and consortial based systems have been greatly emphasized.In this context,possible strategies are also discussed to give an insight into microbial engineering for improved pro-duction of natural products in the future.In summary,it is concluded that the coupling of genomic modifications with optimum physicochemical factors would be promising for producing a robust microbial cell factory that shall contribute to the increased production of natural products. | Muhammad Hammad Hussain Muhammad Zubair Mohsin Waqas Qamar Zaman Junxiong Yu Xueli Zhao Yanlong Wei Yingping Zhuang Ali Mohsin Meijin Guo | 2022 | Synthetic and Systems Biotechnology2022，7，1： | 0 |
| 11 | Continuous-flow enzymatic synthesis of chiral lactones in a three-dimensional microfluidic reactor显示文摘A new continuous-flow process for the enzymatic synthesis of optically pureγ-lactones,which are used as flavors and fragrances in the food and cosmetic industries,was developed in a three-dimensional microfluidic reactor.The microchannels(175 mm in length,0.9 mm in depth,and 1.72 mL in volume)were carved precisely inside a single borosilicate glass(90 mm×75 mm×12 mm)with ultrafast femtosecond laser micromachining.The flow field analysis and reaction simulation showed that the mixing of substrates and enzymes was enhanced,allowing the adjustment of residence time in a wide window.SmCR_(V4),a carbonyl reductase with excellent catalytic activity and enantioselectivity towardγ/δ-keto acids,was employed for the asymmetric synthesis of various chiral lactones.30 mmol/L(R)-γ-decalactone(3g)can be obtained in 26 s with a space-time yield(STY)up to 16,877 g L^(-1)d^(-1),which is 14.4 times higher than the highest STY of batch reaction reported previously.This continuous-flow process was applied to the synthesis of 6 chiral lactones.In addition,the scaled-up synthesis of 3g was carried out in 6 cascade microreactors continuously for 6 h,demonstrating the feasibility and stability of the 3D continuous-flow process in enzymatic synthesis of optically pure compounds. | Xuelei Deng Meng Fan Miao Wu Xiaoyan Zhang Ya Cheng Jianye Xia Yingping Zhuang Weiping Zhu Xuhong Qian Yunpeng Bai | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 12 | Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate(MVA)and lycopene expression pathways显示文摘The stability and high productivity of heterogeneous terpenoid production in Escherichia coli expression system is one of the most key issues for its large scale industrialization.In the current study on taking lycopene biosynthesis as an example,an integrated Escherichia coli system has been generated successfully,which resulted into stable and high lycopene production.In this process,two modules of mevalonate(MVA)pathway and one module of lycopene expression pathway were completely integrated in the chromosome.Firstly,the copy number and integrated position of three modules of heterologous pathways were rationally optimized.Later,a strain DH416 equipped with heterogeneous expression pathways through chromosomal integration was efficiently derived from parental strain DH411.The evolving DH416 strain efficiently produced the lycopene level of 1.22 g/L(49.9 mg/g DCW)in a 5 L fermenter with mean productivity of 61.0 mg/L/h.Additionally,the integrated strain showed more genetic stability than the plasmid systems after successive 21st passage. | Muhammad Hammad Hussain Qi Hong Waqas Qamar Zaman Ali Mohsin Yanlong Wei Ning Zhang Hongqing Fang Zejian Wang Haifeng Hang Yingping Zhuang Meijin Guo | 2021 | Synthetic and Systems Biotechnology2021，6，2： | 0 |
| 13 | High-expression of recombinant human concensus interferon-α by Pichia pastoris显示文摘The present work focused on the high expression of recombinant human consensus interferon-α(cIFN)by Pichia pastoris.The cycle of glycerol feeding,the strategy of methanol feeding and the optimum pH for protein induction were studied.The optimized strategies were a 4-h glycerol-feeding period,induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L.The maximum dry cell weight,cIFN production and bioactivity obtained were 168,1.24 g/L and 5.4×10^(7) U/mL,respectively. | HAO Yuyou SHI Qiqi HE Yun ZHUANG Yingping WANG Yonghong ZHANG Siliang CHU Ju LIU Zhimin | 2007 | Frontiers of Chemical Science and Engineering2007，1，4： | 0 |
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