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| 1 | Prognostic significance of combining high mobility group Box-1 and OV-6 expression in hepatocellular carcinoma显示文摘The inflammatory environment and existence of cancer stem cells are critical for progression and intrahepatic recurrence of hepatocellular carcinoma(HCC) after curative resections. Here, we investigated the prognostic significance of combining high mobility group box 1(HMGB1) expression and hepatic progenitor marker OV6 in hepatocellular carcinoma. Expression of HMGB1 and OV6 was evaluated using immunohistochemistry profiling in tissue microarrays containing samples from 208 HCC patients. Invasive clinical or pathological factors were found in patients with high expression of HMGB1 or OV6. Higher HMGB1 was associated with poorer clinical outcomes, and independently related to elevated 5-year recurrence incidence(85.5% vs. 62.4%, P<0.001). We also found that more OV6 positive staining was correlated with poor prognosis of HCC patients(P<0.001). Notably, expression of HMGB1 was positively correlated with OV6 in density(R2=0.032, P<0.001) and reversely related to HCC outcomes. Abnormal expression of HMGB1 in combination with positive staining of OV6 displayed poorer prognostic performance than single biomarker alone(area under curve(AUC) survival=0.696). Therefore, HMGB1 and OV6 positive staining are promising prognostic parameters for HCC, and we propose that HMGB1 and OV6 may cooperate with each other and predict poor prognosis of HCC. | Jihui Zhu Han Yu Shuzhen Chen Pinghua Yang Zihui Dong Yan Ling Hao Tang Shilei Bai Wen Yang Liang Tang Feng Shen Hongyang Wang Wen Wen | 2018 | Science China(Life Sciences)2018，61，8： | 4 |
| 2 | ASB3 knockdown promotes mitochondrial apoptosis via activating the interdependent cleavage of Beclin1 and caspase-8 in hepatocellular carcinoma显示文摘Apoptosis and autophagy are distinct cellular processes that can be highly interconnected. The cross talk between the two processes is indispensable in determining the overall cell fate. Although the apoptosis-promoting effect of caspases has been demonstrated, the roles of autophagy-related proteins and even autophagy itself in regulating apoptosis remain poorly understood. In our present study, we found that downregulation of ubiquitin E3 ligase ASB3 led to enhanced mitochondrial apoptosis as well as autophagy, which synergistically promoted cell death in hepatocellular carcinoma(HCC). We observed the activation of caspase-8 and decrease of autophagy protein Beclin1 in apoptotic cells that were depleted of ASB3. Beclin1 was mainly cleaved by activated caspase-8 and active Beclin1 initiated mitochondrial apoptosis via locating its C-terminal fragment to mitochondria. In addition, knocking down of Beclin1 markedly blocked the apoptosis, indicating its essential role in the process.Notably, our study indicated that enhanced autophagy level might be involved in the activation of caspase-8 and promote the apoptosis. Taken together, our results demonstrated that ASB3 can regulate mitochondrial pathway of apoptosis by controlling caspase-8 mediated cleavage of Beclin1 in HCC. Therefore, ASB3 may potentially serve as a novel target for HCC therapy,especially when combined with autophagy agonist. | Wenli Zhang Fuchen Liu Zhihui Che Mengmeng Wu Zihui Tang Jie Liu Dongqin Yang | 2019 | Science China(Life Sciences)2019，62，12： | 3 |
| 3 | S-nitrosylation of EMMPRIN influences the migration of HSCs and MMP activity in liver fibrosis显示文摘The mechanism of extracellular matrix metalloproteinase inducer(EMMPRIN)in the regulation of liver fibrosis has not been clarified.This study aims to investigate the role of EMMPRIN S-nitrosylation(SNO)in the regulation of hepatic stellate cell(HSC)migration and matrix metalloproteinase(MMP)activities in liver fibrosis.The results from the tissue microarrays and rat/mouse liver tissues suggest that EMMPRIN mRNA and protein levels in the fibrotic livers are lower than those in the corresponding normal control livers,but higher SNO level of EMMPRIN in fibrotic liver area was shown by immunohistochemistry,immunofluorescence staining,and biotin-switch assay conversely in vivo.Primary EMMPRIN comes from hepatocytes and liver sinus epithelial cells(LSECs)rather than quiescent HSCs.To mimic the uptake of extrinsic EMMPRIN,supernatants from mouse primary hepatocytes/293 cells transfected with EMMPRIN wild-type plasmids(WT)and EMMPRIN SNO site(cysteine 87)mutation plasmids(MUT)were collected and added to JS-1/LX2 cell medium.The MUT EMMPRIN diminishes SNO successfully,enhances the activities of MMP2 and MMP9,and subsequently increases HSC migration.In conclusion,SNO of EMMPRIN influences HSC migration and MMP activities in liver fibrosis.This finding may shed light on the possible regulatory mechanism of MMPs in ECM accumulation in liver fibrosis. | Xinyan Zhu Zihui Tang Wei Li Xiaojuan Li Yasuko Iwakiri Fei Liu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，10： | 0 |
| 4 | Kidney-Qi deficiency diagnosed by Deng's diagnosis standard was not correlated with aging based on clinical observation of 90 participants显示文摘OBJECTIVE: To verify the Traditional Chinese Medicine(TCM) theory that kidney-Qi deficiency(KQD)is considered to be the main cause of aging using cross-sectional study.METHODS: Demographic and lifestyle characteristics of 90 healthy participants were collected with a self-administered questionnaire. KQD syndrome was diagnosed according to Deng's diagnosis standard. Creatinine-adjusted urinary 8-hydroxy-2'-deoxyguanosine(8-OH-dG) and 8-isomeric-prostaglandin2α(8-iso-PGF2α), salivary advanced oxidation protein products(AOPPs), malondialdehyde(MDA) and dehydroepiandrosterone-sulfate(DHEA-S) were selected as aging markers and measured using enzyme-linked immunosorbent assay.RESULTS: No significant differences were observed in participant characteristics between the KQD group and non-KQD(NKQD) group(P > 0.05). Levels of 8-OH-dG, 8-iso-PGF2α, AOPPs, and MDA increased with age, except for a slight decrease in8-OH-dG in the older group. The increase in8-iso-PGF2α was significant(P < 0.05). DHEA-S significantly decreased with increasing age(P < 0.01).8-OH-dG levels were higher in the KQD group compared with the NKQD group. Levels of urinary8-iso-PGF2α, salivary AOPPs, and MDA in the KQD group were lower than in the NKQD group. Salivary DHEA-S was higher in the KQD group compared with the NKQD group. However, differences between KQD group and NKQD group were not significant.CONCLUSION: The current results suggested that KQD syndrome, as diagnosed by Deng's standard,does not underlie the aging phenotype. | Zhao Zhengxiao Liu Lumei Tang Zihui Mohammadtursun Nabijan Lu Linwei Li Mihui Lü Yibao Mo Shuming Ma Wenjuan Wang Meng Wei Ying Gong Weiyi Dong Jingcheng | 2019 | Journal of Traditional Chinese Medicine2019，39，2： | 0 |
| 5 | Bivariate association analysis for quantitative traits using generalized estimation equation显示文摘Quantitative traits often underlie risk for complex diseases.Many studies collect multiple correlated quantitative phenotypes and perform univariate analyses on each of them respectively.However,this strategy may not be powerful and has limitations to detect pleiotropic genes that may underlie correlated quantitative traits.In addition,testing multiple traits individually will exacerbate perplexing problem of multiple testing.In this study,generalized estimating equation 2(GEE2) is applied to association mapping of two correlated quantitative traits.We suppose that a quantitative trait locus is located in a chromosome region that exerts pleiotropic effects on multiple quantitative traits.In that region,multiple SNPs are genotyped.Genotypes of these SNPs and the two quantitative traits affected by a causal SNP were simulated under various parameter values:residual correlation coefficient between two traits,causal SNP heritability,minor allele frequency of the causal SNP,extent of linkage disequilibrium with the causal SNP,and the test sample size.By power analytical analyses,it is showed that the bivariate method is generally more powerful than the univariate method.This method is robust and yields false-positive rates close to the preset nominal significance level.Our real data analyses attested to the usefulness of the method. | Fang Yang Zihui Tang Hongwen Deng | 2009 | Journal of Genetics and Genomics2009，36，12： | 0 |
| 6 | Novel azobenzene-phthalocyanine dyads——design of photo-modulated J-aggregation显示文摘Based on the J-aggregation mechanism of α-aryl/alkoxy-substituted zinc phthalocyanines(Pcs) in non-coordinating solvents, two novel azobenzene-phthalocyanine dyads (3-azo-ZnPc and 4-azo-ZnPc) were synthesized with the aim of developing Pc compounds whose ability to form J-aggregation could be photo-modulated. It was found that 3-azo-ZnPc in chloroform could be effectively photo-controlled in a wide range. This phenomenon could be explained by the changes in the geometry and dipole moment of azobenzene during the photo-isomerization process. 4-azo-ZnPc did not have this ability at all, with or without UV light illumination. The positions of the oxygen atoms to which the aryl/alkoxy substitution was attached relatively were found important in determining the aggregation ability. | NIU LiHong ZHONG Cheng CHEN ZiHui ZHANG Zhi LI ZhongYu ZHANG FuShi TANG YingWu | 2009 | Chinese Science Bulletin2009，54，7： | 0 |
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