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| 1 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 2 | Ambulatory blood pressure profile and stroke recurrence显示文摘Objectives To establish a new ambulatory blood pressure(ABP)parameter(24-hour ABP profile)and evaluated its performance on stroke outcome in ischaemic stroke(IS)or transient ischaemic attack(TIA)patients.Methods The prospective cohort consisted of 1996 IS/TIA patients enrolled for ABP monitoring and a 3-month follow-up for stroke recurrence as outcome.Profile groups of systolic blood pressure(SBP)were identified via an advanced functional clustering method,and the associations of the profile groups and conventional ABP parameters with stroke recurrence were examined in a Cox proportional hazards model.Results Three discrete profile groups(n=604,781 and 611 in profiles 1,2 and 3,respectively)in 24-hour ambulatory SBP were identified.Profile 1 resembled most to the normal diurnal blood pressure pattern;profile 2 also dropped at night,but climbed earlier and with higher morning surge;while profile 3 had sustained higher nocturnal SBP without significant nocturnal SBP decline.The incidence of stroke recurrence was 2.9%,3.9%and 5.5%in profiles 1,2 and 3,respectively.After adjustment for covariates,profile 3 was significantly associated with higher risk of stroke recurrence with profile 1 as reference(HR 1.76,95%CI:1.00 to 3.09),while no significant difference was observed between profiles 2 and 1(HR 1.22,95%CI:0.66 to 2.25).None of conventional ABP parameters showed significant associations with the outcome.Conclusions Ambulatory 24-hour SBP profile is associated with short-term stroke recurrence.Profiles of ABP may help improve identification of stroke recurrence by capturing the additive effects of individual ABP parameters. | Jie Xu Fei Jiang Anxin Wang Hui Zhi Yuan Gao Junping Tian Jinglin Mo Zimo Chen An-Ding Xu Benyan Luo Bo Hu Yuqing Zhang Xingquan Zhao Yilong Wang Hao Li Haipeng Shen Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，3： | 3 |
| 3 | One versus two BAK fusion cages in posterior lumbar interbody fusion to L4/L5 degenerative spondylo- listhesis: a randomized controlled prospective study in 25 patients with minimum two-year followed-up 显示文摘 | Zimo J Wang X Hou T | 2002 | Spine2002，27，24： | 1 |
| 4 | Global analysis of an SIS model with an infective vector on complex networks显示文摘 | Yi Wang Zhen Jin Zimo Yang Zi-Ke Zhang Tao Zhou Gui- Quan Sun | 2012 | Nonlinear Analysis: RealWorld Applica- tions2012，2012，： | 1 |
| 5 | A likely autotetraploidization event shaped the Chinese mahogany(Toona sinensis)genome显示文摘Chinese mahogany(Toona sinensis) is of considerable medical and economic importance, and its genome has been deciphered. However, the process underlying its polyploidy is unclear, and the chromosomal evolutionary trajectory is poorly understood. Here, by reanalysing the T.sinensis genome, we found evidence of a tetraploidization event(T. sinensis special tetraploidization, TST) that occurred approximately 15-17 million years ago(MYA) after the core eudicot-common hexaploidization(ECH or gamma) event. We characterized the synonymous nucleotide substitution rates(Ks values) of collinear genes and found that T. sinensis genes affected by the TST evolve at a slower rate than Acer yangbiense genes. Furthermore, we identified homologous genes related to polyploidization and speciation and constructed multiple alignments with different reference genomes. Notably, the significant balance of gene retention and loss characterized in the two TST-derived subgenomes suggests an autopolyploid nature of the TST. Moreover, we deduced the chromosomal karyotypes of the two subgenomes and identified 7chromosomal fusions that have shaped the T. sinensis genome;more information is available on a newly constructed karyotype platform(http://gffzze84c5b42976d4cd7hfvuoc6qpcqpx65of.ffgz.tsg.suse.edu.cn/Toona_sinensis/index.html). The T. sinensis genome preserves the ancestral chromosome structure of dicotyledons well and could serve as a good reference for understanding genomic changes in other Meliaceae and Sapindales plants. In addition, we verified that tandem duplication and the ECH have promoted the expansion of terpene synthase(TPS) genes;conversely, the TST seems to have inhibited expansion of these genes. This present effort has clarified the polyploidy events of the T. sinensis genome, filled gaps in the history of karyotype evolution, and laid a solid foundation for further genomic studies in the Meliaceae research community and beyond. | Jianyu Wang Ziyi Yang Tianyu Lei Yan Zhang Qimeng Xiao Zijian Yu Jiaqi Zhang Sangrong Sun Qiang Xu Shaoqi Shen Zimo Yan Mengnan Fang Yue Ding Zihan Liu Qianwen Zhu Ke Ren Yuxin Pan Haibin Liu Jinpeng Wang | 2023 | Horticultural Plant Journal2023，9，2： | 0 |
| 6 | Low LDL-C level and intracranial haemorrhage risk after ischaemic stroke:a prospective cohort study显示文摘Background and purpose The Treat Stroke to Target trial has confirmed the benefit of targeting low-density lipoprotein cholesterol(LDL-C)of<1.8 mmol/L in patients who had an ischaemic stroke(IS).However,haemorrhagic risk brought by this target level(<1.8 mmol/L)or even lower level(<1.4 mmol/L)of LDL-C should also be concerned.In this study,we aimed to demonstrate whether low LDL-C could increase the intracranial haemorrhage risk following IS.Methods Patients who had an IS from China Stroke Center Alliance programme with complete baseline information were prospectively enrolled.793572 patients who had an IS were categorised into 6 groups according to LDL-C level(<1.40 mmol/L,1.40-1.79 mmol/L,1.80-2.59 mmol/L,2.60-2.99 mmol/L,3.00-4.89 mmol/L,≥4.90 mmol/L).The study outcome was defined as intracranial haemorrhage identified during hospitalisation.Logistic regression model was used to examine the association between different LDL-C levels and risk of intracranial haemorrhage.Results Compared with patients of LDL-C=1.80-2.59 mmol/L,both subgroups of LDL-C<1.40 mmol/L and LDL-C=1.40-1.79 mmol/L showed significantly higher risk of intracranial haemorrhage(OR=1.26,95%CI=1.18 to 1.35;OR=1.22,95%CI=1.14 to 1.30,respectively).Interaction effect was found to exist between the subgroups of intravenous thrombolytic therapy(p=0.04),rather than the subgroups of age,sex and body mass index.Moreover,the sensitivity analyses indicated that even patients who had an IS with minor stroke still suffered from the increased intracranial haemorrhage risk related to low LDL-C level.Conclusions Among patients who had an IS,the low LDL-C level(<1.4 mmol/L or<1.8 mmol/L)at baseline is associated with increased risk of intracranial haemorrhage during acute stage.While actively lowering LDL-C level for patients who had an IS,clinicians should also concern about the haemorrhagic risk associated with low LDL-C level. | Jie Xu Zimo Chen Meng Wang Jinglin Mo Jing Jing Gulbahram Yalkun Liye Dai Xia Meng Hao Li Zixiao Li Yongjun Wang | 2023 | Stroke & Vascular Neurology2023，8，2： | 0 |
| 7 | China's Distinctive Mark and World Significance of G20 Hangzhou Summit显示文摘Engagement at the West Lake attracts world’s attention.On September 4-5,2016,under the auspices of Chinese President Xi Jinping,the11th Group of 20(G20)Summit is held in Hangzhou,China.How to deal with the challenges of the world economy,what kind of program China proposes for the | Yu Xiaokui Wang Zimo | 2016 | Peace2016，，3： | 0 |
| 8 | Cumulative burden of lipid profiles predict future incidence of ischaemic stroke and residual risk显示文摘Objectives Incident ischaemic stroke(IS)risk may increase not only with lipids concentration but also with longer duration of exposure.This study aimed to investigate the impact of cumulative burden of lipid profiles on risk of incident IS.Methods A total of 43836 participants were enrolled who participated in four surveys during 2006-2013.Individual cumulative lipid burden was calculated as number of years(2006-2013)multiplied by the levels of low-density lipoprotein cholesterol(LDL-C),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),non-HDL C and triglyceride(TG),respectively.The primary outcome was defined as the incident IS during 2012-2017.Results During 4.67 years(±0.70 years)follow-up on average,we identified 1023(2.33%)incident IS.Compared with respective reference groups,the HRs(95%CIs)of the upper tertile in cumulative TG burden,cumulative LDL-C burden,cumulative TC burden and cumulative non-HDL C burden were 1.26 mmol/L(1.02-1.55 mmol/L),1.47 mmol/L(1.25-1.73 mmol/L),1.33 mmol/L(1.12-1.57 mmol/L)and 1.51 mmol/L(1.28-1.80 mmol/L)for incidence of IS,respectively.However,this association was not significant in cumulative HDL-C burden and IS(HR:1.09;95%CI:0.79 to 1.52),after adjustment for confounding variables.Among 16600 participants with low cumulative LDL-C burden,HRs(95%CI)for TC,TG,non-HDL C and HDL-C with IS were 1.63 mmol/L(1.03-2.57 mmol/L),1.65 mmol/L(1.19-2.31 mmol/L),1.57 mmol/L(1.06-2.32 mmol/L)and 0.98 mmol/L(0.56-1.72 mmol/L),respectively.Conclusions We observed the correlation between cumulative burden of lipid profiles,except for cumulative burden of HDL-C,with the risk of incident IS.Cumulative burden of TC,TG and non-HDL C may still predict IS in patients with low cumulative LDL-C burden.Trial registration number ChiCTR-TNRC 11001489. | Liye Dai Jie Xu Yijun Zhang Anxin Wang Zimo Chen Jinglin Mo Hao Li Xia Meng Shouling Wu Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，4： | 0 |
| 9 | Synthesis of Quaternary Carbon-Centered Benzoindolizidinones via Novel Photoredox-Catalyzed Alkene Aminoarylation: Facile Access to Tylophorine and Analogues显示文摘Photoredox-catalyzed aminoarylation and thioami-nation of unactivated alkenes have been developed,providing novel synthetic routes to access synthe-tically challenging quaternary carbon-centered benzoindolizidinones and trifluoromethylthiolated piperidines using readily available starting materials.Notably,these transformations were enabled by merging amidyl radical generation from N-alkyl benzamides with oxidant incorporation.Density functional theory calculations were performed to understand the reaction mechanism and to rationa-lize the regioselectivities.Moreover,the newly deve-loped catalytic aminoarylation provided a convenient synthetic route for natural product tylophorine and its gem-dimethyl analogues with greatly improved drug-like properties such as enhanced solubility and stability. | Chao Zhang Yi Wang Yugang Song Hongying Gao Yonghui Sun Xiuyun Sun Yiqing Yang Ming He Zimo Yang Lingpeng Zhan Zhi-Xiang Yu Yu Rao | 2019 | CCS Chemistry2019，1，4： | 0 |
| 10 | A New Micropropagation Technology of Tilia amurensis:In VitroMicropropagation of Mature Zygotic Embryos and the Establishment of a PlantRegeneration System显示文摘Tilia amurensis is an economically valuable broadleaf tree species in Northeast China.The production of highqualityT.amurensis varieties at commercial scales has been greatly limited by the low germination rates.Thereis thus a pressing need to develop an organogenesis protocol for in vitro propagation of T.amurensis to alleviate ashortage of high-quality T.amurensis seedlings.Here,we established a rapid in vitro propagation system forT.amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture mediain different stages.We found that Woody plant medium(WPM)was the optimal primary culture medium formature zygotic embryos.The highest callus induction percentage(68.76%)and number of axillary buds induced(3.2)were obtained in WPM+0.89μmol/L 6-benzyladenine(6-BA)+0.46μmol/L kinetin(KT)+0.25μmol/Lindole-3-butryic acid(IBA)+1.44μmol/L gibberellin A_(3)(GA_(3)).The multiple shoot bud development achievedthe highest percentage(83.32%)in the Murashige and Skoog(MS)+2.22μmol/L 6-BA+0.25μmol/L IBA+1.44μmol/L GA_(3).The rooting percentage(96.70%)was highest in 1/2 MS medium+1.48μmol/L IBA.Thesurvival percentage of transplanting plantlets was 82.22%in soil:vermiculite:perlite(5:3:1).Our study is the firstto establish an effective organogenesis protocol for T.amurensis using mature zygotic embryos. | Shijie Lin Zimo Wang Hongbo Zhu Conghui Wang Hongfeng Wang Dawei Zhang Tianbing Gou Guangdao Bao Ye Luo Huaijiang He Zhonghui Zhang | 2024 | Phyton-International Journal of Experimental Botany2024，93，2： | 0 |
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