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| 1 | Associations Between Specific Diets, Dietary Diversity, and Cognitive Frailty in Older Adults — China, 2002–2018显示文摘Summary What is already known about this topic?The relationship between specific dietary patterns and dietary diversity with cognitive frailty continues to be a subject of ambiguity.What is added by this report?This research revealed that regular consumption of fruit,meat,bean products,garlic,and tea was connected to a decreased risk of cognitive frailty.Compared to participants with dietary diversity score(DDS)≤6 points,those with DDS of 9–10,11–12,and≥12 had a lower risk of cognitive frailty. | Qingmei Huang Wenfang Zhong Ziting Chen Zhihao Li Peidong Zhang Yujie Zhang Peiliang Chen Qi Fu Weiqi Song Yuebin Lyu Xiaoming Shi Chen Mao | 2023 | China CDC weekly2023，5，39： | 1 |
| 2 | Molecular mechanisms of Streptococcus pyogenes Cas9:a single-molecule perspective显示文摘Cas9 is an RNA-guided endonuclease from the type II CRISPR-Cas system that employs RNA–DNA base pairing to target and cleave foreign DNA in bacteria.Due to its robust and programmable activity,Cas9 has been repurposed as a revolutionary technology for wide-ranging biological and medical applications.A comprehensive understanding of Cas9 mechanisms at the molecular level would aid in its better usage as a genome tool.Over the past few years,single-molecule techniques,such as fluorescence resonance energy transfer,DNA curtains,magnetic tweezers,and optical tweezers,have been extensively applied to characterize the detailed molecular mechanisms of Cas9 proteins.These techniques allow researchers to monitor molecular dynamics and conformational changes,probe essential DNA–protein interactions,detect intermediate states,and distinguish heterogeneity along the reaction pathway,thus providing enriched functional and mechanistic perspectives.This review outlines the single-molecule techniques that have been utilized for the investigation of Cas9 proteins and discusses insights into the mechanisms of the widely used Streptococcus pyogenes(Sp)Cas9 revealed through these techniques. | Qian Zhang Ziting Chen Bo Sun | 2021 | Biophysics Reports2021，7，6： | 1 |
| 3 | Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer显示文摘Dear Editor,Base editing has been developed to be one of the effective CRISPR-based gene editing techniques(Rees and Liu,2018).Base editors(BEs)take advantage of DNAmodifying enzymes fused with a catalytically impaired CRISPR protein to install base mutations.In BE,DNA-modifying enzymes often process nontarget singlestranded DNA(NTS)generated by Cas proteins. | Ziting Chen Xiangyang Li Qian Zhang Wenjun Sun Xiaoxuan Song Xia Zhang Xingxu Huang Bo Sun | 2024 | Science China(Life Sciences)2024，67，2： | 0 |
| 4 | Real-world outcomes of niraparib treatment in patients with ovarian cancer:amulticenter non-interventional study in China显示文摘Dear Editor,Ovarian cancer remains the deadliest among all gynecological cancers.Although most patients at advanced stage respond to initial treatment,the majority experience recurrence[1].It was estimated that 55,342 new cases and 37,519 deaths from ovarian cancer occurred in China annually[2].Contemporarily,the treatment landscape has changed rapidly since the role of poly(adenosine diphosphate-ribose)polymerase inhibitors(PARPi)in ovarian cancer treatmentwas explored.Based on data from the PRIMA/ENGOT-OV26/GOG-3012[3]and ENGOTOV16/NOVA trials[4],niraparib has been approved globally as maintenance therapy for newly diagnosed and platinum-sensitive recurrent ovarian cancer.The indication of niraparib for salvage treatment was based on the results of the QUADRA(NCT02354586)trial[5].Although niraparib has been tested in prospective randomized clinical trials(RCTs),no multicenter study on its real-world application in China had been conducted.Considering the differences in population,accessibility and affordability of drugs,the results of the real-world settings may differ from those of RCTs.Therefore,we conducted this multicenter,non-interventional study at 8 hospitals. | Jin Li Jianhua Yang Huafeng Shou Lin Zhang Xiaohong Huang Xuedong Tang Fei Zheng Fang Liu Hao Wen Huijuan Yang Huaying Wang Ziting Li Xiaojun Chen Xingzhu Ju Xi Cheng Jie Tang Meiqin Zhang Xiaohua Wu | 2023 | Cancer Communications2023，43，6： | 0 |
| 5 | Overexpression of maize GOLDEN2 in rice and maize calli improves regeneration by activating chloroplast development显示文摘Golden2(G2), a member of the GARP transcription factor superfamily, regulates several biological processes and phytohormone signaling pathways in plants. In this study, we used a rice codon-optimized maize G2 gene(rZmG2) to improve the regeneration efficiency of rice and maize calli for genetic transformation. We isolated a promoter driving strong and callus-specific expression from rice to drive rZmG2 transcription from a transgene after transformation of two indica and two japonica rice cultivars. The resulting rZmG2 transgenic calli turned green in advance at the differentiation stage, thus significantly raising the regeneration rates of the transgenic indica and japonica rice plants relative to control transformations. Similar effect of this gene on improving maize transformation was also observed. Transcriptome sequencing and RT-qPCR analyses showed that many rice genes related to chloroplast development and phytohormones are upregulated in rZmG2-transgenic calli. These results demonstrate that rZmG2 can promote embryogenic callus differentiation and improve regeneration efficiency by activating chloroplast development and phytohormone pathways. We also established a heat-inducible Cre/loxP-based gene-excision system to remove rZmG2 and the antibiotic selectable gene after obtaining the transgenic plants. This study provides a useful tool for functional genomics work and biotechnology in plants. | Wanni Luo Jiantao Tan Tie Li Ziting Feng Zhi Ding Xianrong Xie Yuanling Chen Letian Chen Yao-Guang Liu Qinlong Zhu Jinxing Guo | 2023 | Science China(Life Sciences)2023，66，2： | 0 |
| 6 | Pegylated Liposomal Doxorubicin as a Single Agent or as Combination Therapy with Carboplatin in Patients with Recurrent or Refractory Epithelial Ovarian Cancer显示文摘OBJECTIVE Pegylated liposomal doxorubicin (PLD;CAELYX^(?)), a novel formulation of doxorubicin with enhancedtherapeutic efficacy and reduced toxicity, has demonstratedimproved progression-free survival in recurrent or refractoryovarian cancer. The objective of this open-label, noncomparative,observational study was to determine the efficacyand safety of PLD monotherapy or combination therapy withcarboplatin for patients with recurrent or refractory ovariancancer.METHODS Sixty-two patients with recurrent or refractoryovarian cancer who completed a platinum-based chemotherapyregimen and demonstrated platinum sensitivity for first-linetreatment at least 6 months prior to study entry were enrolledin 20 centers in China. PLD was given as monotherapy (50mg/m^2 infused over 60 minutes) or as combination therapy(30 mg/m^2 1-hour infusion) with carboplatin (area under thecurve 5 mg.min/mL 1-hour infusion) on day 1 every 28 daysfor 4 cycles. The primary endpoint was objective response (OR)rate or CA-125 level. Secondary endpoints included time toresponse, time-to-progression, health-related quality of life, andsafety.RESULTS Overall, 48% of the 62 evaluable patients achieveda confirmed OR. More patients receiving PLD and carboplatinachieved an OR vs the PLD monotherapy group (63% vs. 37%).The median time to response and disease progression was58.5 days and 56.0 days, respectively. Overall and drug-relatedadverse events were reported for 39% and 34%, respectively.The most commonly reported adverse events were stomatitis(22.6%) and palmar-plantar erythroderma (9.7%). Two deathswere reported.CONCLUSION PLD is an effective and well tolerated agentin women with recurrent or refractory epithelial ovarian cancer. | Beihua Kong Yunong Gao Lingying Wu Ziting Li Yile Chen Mengda Li Yongliang Gao Ding ga Zhilan Peng KengShen | 2009 | Clinical oncology and cancer resexreh2009，6，6： | 0 |
| 7 | Gypenosides ameliorate morphine-induced immunosuppression with an increased proportion of thymic T lymphocyte subsets and are involved in the regulation of the cAMP-CREM/CREB-IL-2 pathway显示文摘Opioid abuse can suppress the lymphatic system function,and produce severe immunosuppression that poses a significant risk of opportunistic infections such as methicillinresistant Staphylococcus aureus(MRSA)pneumonia.^(1,2)Gypenosides(Gps)are the most important immunomodulator components in the Chinese herbal medicine Gynostemma pentaphyllum. | Hui Wang Zhonghao Li Qisheng Wang Weixin Lin Ziting Zhou Xinru Mu Yongwei Jiang Shengfeng Lu Shaodong Chen Zhigang Lu | 2024 | Genes & Diseases2024，11，3： | 0 |
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