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| 1 | Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community显示文摘类 Vibrio 原因 vibriosis 的病原的种, maricultured 动物和海味消费者的最流行的疾病之一。在海味生产的链监视他们的动力学,处理并且消费为食物和海洋生物养殖安全是很重要的。为了充实代表 Vibrio 16S ribosomal RNA 基因(rDNA ) ,碎裂并且识别进一步真实及时的这些细菌并且同时地在在链的细菌的植物群之中,有选择地放大 Vibrio 16S rDNA 碎片的一双教材与他们在海水 Vibrio 的分析证明的特性和报道被设计社区。二份教材, VF169 和 VR744 的 Thespecificities 和范围,被使用强风节目在在 GenBank 可得到的细菌的 16S rDNAs 之中理论上决定,实际上,由放大 Vibrio16S, rDNA 从海水 DNA 碎裂。超过 88.3% 序列在 GenBank,与 VR744 显示出相同火柴,属于 Vibrio 类。33 克隆的一个总数随机被选择并且定序。所有序列显示出他们的最高的类似到并且多样的已知的 Vibrio 种类在那些附近聚类。设计的教材能够检索宽范围 ofVibrio 16S rDNA 在海水在细菌的植物群之中明确地碎裂,海味耕作的最重要的自然环境。 | LIU Yong YANG Guanpin WANG Hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei SUN Xiuqin | 2006 | Journal of Ocean University of China2006，5，2： | 5 |
| 2 | Field Efficacy Trial and Optimum Control Period of Rice False Smut(Ustilaginoidea virens) in Single Cropping Middle-late Rice显示文摘[Objective]The paper was to study optimum chemical agents and control periods against rice false smut( Ustilaginoidea virens) in single cropping middle-late rice. [Method]Taking chemical agents( triadimefon,armure,Jinggangmycin) as main treatments and spraying periods( within 7 d before initial heading stage,within 7 d before initial heading stage + initial heading stage,initial heading stage) as assisted treatments,the field efficacy trial and optimum control period of U. virens in single cropping middle-late rice were studied using Huanghuazhan and Jinnongsimiao as experimental materials in 2013. [Result] Three chemical agents,triadimefon,armure,Jinggangmycin,extremely reduced diseased panicle rate and disease index of U. virens,but there was no significant difference among three chemical agents. Spraying period did not have significant impact on diseased panicle rate and disease index of U. virens,whereas spraying within7 d before initial heading stage and during initial heading stage had relatively good control effect. Yield increased significantly after application of three chemical agents,and armure led to greater increase. The reason for yield increase was that seed setting rate was significantly increased,and the number of filled grains per panicle was significantly increased. Although spraying period influenced yield,the difference was not significant. [Conclusion] Triadimefon,armure and Jinggangmycin had good control effects against U. virens in single cropping middle-late rice,of which armure had better control effects. Spraying chemical agents increased seed setting rate,and further increased the number of filled grains per panicle and yield. Spraying within 7 d before initial heading stage and during initial heading stage had relatively better control effect against U. virens. | Liu Huaizhen Li Kanghuo Huang Qing Lu Xiuming Zhang Bin Li Huifeng Zou Jixiang Zhou Shaochuan | 2014 | Plant Diseases and Pests2014，5，2： | 1 |
| 3 | Transplantation of neural stem progenitor cells from different sources for severe spinal cord injury repair in rat显示文摘Neural stem progenitor cell(NSPC)transplantation has been regarded as a promising therapeutic method for spinal cord injury(SCI)repair.However,different NSPCs may have different therapeutic effects,and it is therefore important to identify the optimal NSPC type.In our study,we compared the transcriptomes of human fetal brain-derived NSPCs(BNSPCs),spinal cord-derived NSPCs(SCNSPCs)and H9 embryonic stem-cell derived NSPCs(H9-NSPCs)in vitro and subsequently we transplanted each NSPC type on a collagen scaffold into a T8-9 complete SCI rat model in vivo.In vitro data showed that SCNSPCs had more highly expressed genes involved in nerve-related functions than the other two cell types.In vivo,compared with BNSPCs and H9-NSPCs,SCNSPCs exhibited the best therapeutic effects;in fact,SCNSPCs facilitated electrophysiological and hindlimb functional recovery.This study demonstrates that SCNSPCs may be an appropriate candidate cell type for SCI repair,which is of great clinical significance. | Bai Xu Man Yin Yaming Yang Yunlong Zou Wenbin Liu Lianyong Qiao Jixiang Zhang Zhan Wang Yayu Wu He Shen Minghan Sun Weiyuan Liu Weiwei Xue Yongheng Fan Qi Zhang Bing Chen Xianming Wu Ya Shi Falong Lu Yannan Zhao Zhifeng Xiao Jianwu Dai | 2023 | Bioactive Materials2023，，5： | 0 |
| 4 | Chemical Weeding in Dry Direct Seeding Fields of Single Cropping Middlelate Rice显示文摘Chemical weeding in dry direct seeding fields of single cropping middle-late rice was studied in Huida vegetable farm of Huizhou City in 2012. The main treatment was herbicide( pretilachlor + bensulfuron-methyl,Yangguo and butachlor),and the sub-treatment was application method( soil treatments,seedling treatment and integrated treatment). The results showed that 80 g pretilachlor + bensulfuron-methyl( 36% pretilachlor + 4% bensulfuron-methyl) diluted with 50 kg water could be sprayed or 200 g Yangguo( 23. 9% butachlor + 1. 1% bensulfuron-methyl) mixed with 15 kg sandy soil could be broadcasted per 667 m2 on the sowing day or the second day under moist condition of soil,which could effectively control weeds in dry direct seeding fields of single cropping middle-late rice. | Liu Huaizhen Li Kanghuo Huang Qing Lu Xiuming Zhang Bin Li Huifen Zou Jixiang Zhou Shaochuan | 2014 | Plant Diseases and Pests2014，5，3： | 0 |
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