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| 1 | Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers显示文摘Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS', where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR_1, RhA G, and RhNUDX_1 in rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods. | Huijun Yan Shaochuan Shi Nan Ma Xiaoqian Cao Hao Zhang Xianqin qiu Qigang Wang Hongying Jian Ningning Zhou Zhao Zhang Kaixue Tang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 11 |
| 2 | From Green Super Rice to green agriculture:Reaping the promise of functional genomics research显示文摘Producing sufficient food with finite resources to feed the growing global population while having a smaller impact on the environment has always been a great challenge.Here,we review the concept and practices of Green Super Rice(GSR)that have led to a paradigm shift in goals for crop genetic improvement and models of food production for promoting sustainable agriculture.The momentous achievements and global deliveries of GSR have been fueled by the integration of abundant genetic resources,functional gene discoveries,and innovative breeding techniques with precise gene and whole-genome selection and efficient agronomic management to promote resource-saving,environmentally friendly crop production systems.We also provide perspectives on new horizons in genomic breeding technologies geared toward delivering green and nutritious crop varieties to further enhance the development of green agricul-ture and better nourish the world population. | Sibin Yu Jauhar Ali Shaochuan Zhou Guangjun Ren Huaan Xie Jianlong Xu Xinqiao Yu Fasong Zhou Shaobing Peng Liangyong Ma Dingyang Yuan Zefu Li Dazhou Chen Ruifeng Zheng Zhigang Zhao Chengcai Chu Aiqing You Yu Wei Susong Zhu Qiongyao Gu Guangcun He Shigui Li Guifu Liu Changhua Liu Chaopu Zhang Jinghua Xiao Lijun Luo Zhikang Li Qifa Zhang | 2022 | Molecular Plant2022，15，1： | 6 |
| 3 | Identification of Nonstructural Protein 8 as the N-Terminus of the RNA-Dependent RNA Polymerase of Porcine Reproductive and Respiratory Syndrome Virus显示文摘Porcine reproductive and respiratory syndrome virus (PRRSV) is a member within the family Arteriviridae of the order Nidovirales. Replication of this positive-stranded RNA virus within the host cell involves expression of viral replicase proteins encoded by two ORFs, namely ORF1 a and ORF1 b. In particular, translation of ORF1 b depends on a-1-ribosomal frameshift strategy. Thus, nonstructural protein 9 (nsp9), the first protein within ORF1 b that specifies the function of the viral RNA-dependent RNA polymerase, is expressed as the C-terminal extension of nsp8, a small nsp that is encoded by ORF1 a. However, it has remained unclear whether the mature form of nsp9 in virus-infected cells still retains nsp8,addressing which is clearly critical to understand the biological function of nsp9. By taking advantage of specific antibodies to both nsp8 and nsp9, we report the following findings. (1) In infected cells, PRRSV nsp9 was identified as a major product with a size between 72 and 95 k Da (72–95 KDa form), which exhibited the similar mobility on the gel to the in vitro expressed nsp8–9 ORF1 b, but not the ORF1 b-coded portion (nsp9 ORF1 b). (2) The antibodies to nsp8, but not to nsp7 or nsp10, could detect a major product that had the similar mobility to the 72–95 KDa form of nsp9. Moreover, nsp9 could be co-immunoprecipitated by antibodies to nsp8, and vice versa. (3) Neither nsp4 nor nsp2 PLP2 was able to cleave nsp8–nsp9 in vitro. Together, our studies provide experimental evidence to suggest that nsp8 is an N-terminal extension of nsp9.Our findings here paves way for further charactering the biological function of PRRSV nsp9. | Yuanyuan Liu Yunhao Hu Yue Chai Liping Liu Jiangwei Song Shaochuan Zhou Jia Su Lei Zhou Xinna Ge Xin Guo Jun Han Hanchun Yang | 2018 | Virologica Sinica2018，33，5： | 3 |
| 4 | Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications显示文摘Dextran-hyaluronate (Dex -- 哈) 为软织物工程的基于的 supermacroporous cryogel 脚手架被在结冰条件下面在各种各样的 macromonomer 集中包含葡聚糖 methacrylate (Dex 麻省) 和 hyaluronate methacrylate (哈麻省) 的水的答案的免费激进的 cryo-copolymerization 准备。这被观察为 Dex 的准备的 macromonomers 的合适的全部的集中 -- 哈有满足的性质的 cryogel 支架是 5%(w/w ) 在 1% 的哈麻省集中(w/v ) ,它然后被用来生产测试支架。有 5% 的获得的 cryogel 支架(w/w ) macromonomer 答案有高水位线渗透(5.1 ? 桴 ? 潨灳瑩? | Dongjiao ZHOU Shaochuan SHEN Junxian YUN Kejian YAO Dong-Qiang LIN | 2012 | Frontiers of Chemical Science and Engineering2012，6，3： | 1 |
| 5 | Field Efficacy Trial and Optimum Control Period of Rice False Smut(Ustilaginoidea virens) in Single Cropping Middle-late Rice显示文摘[Objective]The paper was to study optimum chemical agents and control periods against rice false smut( Ustilaginoidea virens) in single cropping middle-late rice. [Method]Taking chemical agents( triadimefon,armure,Jinggangmycin) as main treatments and spraying periods( within 7 d before initial heading stage,within 7 d before initial heading stage + initial heading stage,initial heading stage) as assisted treatments,the field efficacy trial and optimum control period of U. virens in single cropping middle-late rice were studied using Huanghuazhan and Jinnongsimiao as experimental materials in 2013. [Result] Three chemical agents,triadimefon,armure,Jinggangmycin,extremely reduced diseased panicle rate and disease index of U. virens,but there was no significant difference among three chemical agents. Spraying period did not have significant impact on diseased panicle rate and disease index of U. virens,whereas spraying within7 d before initial heading stage and during initial heading stage had relatively good control effect. Yield increased significantly after application of three chemical agents,and armure led to greater increase. The reason for yield increase was that seed setting rate was significantly increased,and the number of filled grains per panicle was significantly increased. Although spraying period influenced yield,the difference was not significant. [Conclusion] Triadimefon,armure and Jinggangmycin had good control effects against U. virens in single cropping middle-late rice,of which armure had better control effects. Spraying chemical agents increased seed setting rate,and further increased the number of filled grains per panicle and yield. Spraying within 7 d before initial heading stage and during initial heading stage had relatively better control effect against U. virens. | Liu Huaizhen Li Kanghuo Huang Qing Lu Xiuming Zhang Bin Li Huifeng Zou Jixiang Zhou Shaochuan | 2014 | Plant Diseases and Pests2014，5，2： | 1 |
| 6 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 7 | Coal Seam Methane Distribution and Its Significance in Pingdingshan Mining Area 显示文摘 | GUO Deyong ZHOU Xinquan SHEN Shaochuan | 2002 | Journal of Coal Science & Engineering2002，8，2： | 1 |
| 8 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 9 | Coal forming environments and their relationship to tectonic activity in the Cevennes Stephanian coal basin显示文摘 | Guo Deyong Zhou Xinquan Shen Shaochuan | 2002 | Journal of coal Science & Engineering ( China )2002，8，： | 1 |
| 10 | Comparisons of Metabolic Profiles for Carbohydrates,Amino Acids,Lipids,Fragrance and Flavones During Grain Development in indica Rice Cultivars显示文摘We used a widely targeted metabolomics approach to examine the metabolic changes in three pedigreed indica cultivars(Meixiangzhan 2,Meisizhan and Qixinzhan),with different eating qualities,at 8,15 and 30 d after flowering(DAF)to explore the formation mechanism of rice eating and nutritional qualities at a global metabolic level.A total of 623 metabolites were identified,and results showed that metabolic variations among rice cultivars decreased with grain developmental stage,suggesting that sufficient carbohydrate and amino acid supply during grain development may contribute to excellent rice eating and nutritional quality formation.Lysophosphatidylcholines 19:0 and 16:1 were beneficial for excellent eating quality formation during grain development.Rice fragrance was attributed mainly to spermidine andγ-aminobutyric acid.Rice cultivars with excellent eating quality at 15-30 DAF had relatively higher flavone content,possibly because they had adequate carbohydrate and amino acid contents during grain development.These results provided insight into the mechanisms for establishing rice eating and nutritional qualities during grain formation at a global metabolic level,and can be applied towards improving rice quality. | CHEN Yibo WANG Zhidong WANG Chongrong LI Hong HUANG Daoqiang ZHOU Degui ZHAO Lei PAN Yangyang GONG Rong ZHOU Shaochuan | 2022 | Rice science2022，29，2： | 0 |
| 11 | Architecture modification and In^(3+)-doping of WO_(3) photoanodes to boost the photoelectrochemical water oxidation performance显示文摘WO_(3) photoanodes have been widely utilized for the oxygen evolution reaction(OER)in the photoelectrochemical water splitting system.Herein,the effects of hydrazine hydrate modification and In^(3+)-doping on the physicochemical properties and photocurrent density of the WO_(3) photoanode prepared by hydrothermally treating at 160℃followed by calcining at 500℃are investigated.Among them,the hydrazine hydrate in the hydrothermal solution can serve as a texture regulator,resulting in the formation of WO_(3) films with the layered architecture stacked by nanosheets dominantly exposed(020)facets,which allows the WO_(3) films to have faster charge separation and larger specific surface area for OER according to the characterization results of microstructures and photoelectrochemcial behaviors;while the In^(3+)-doping can optimize the energy band structure of WO_(3) and adjust the work function to increase the driving force of OER based on the ultraviolet photoelectron spectroscopy,Mott–Schottky and open-circuit photovoltage plots.Under the simulated sunlight(AM1.5G)illumination,the designed In^(3+)–WO_(3)(N_2H_4)photoanode in Na_2SO_4 solution delivers amaximum incident photon-to-current efficiency of 38.6%at 410 nm and a photocurrent density of 1.93 mA cm^(-2)at 1.23 V vs.RHE,which is 2.8 and 3.0 times higher than the pristine WO_(3) photoanode,respectively.This study provides a promising strategy to improve the water splitting performance of nanostructured WO_(3) photoanodes by altering the architecture and introducing heteroatoms. | Peng Zeng Yang Zhou Lingling Peng Shaochuan Wang Tianyou Peng | 2023 | Science China Chemistry2023，66，11： | 0 |
| 12 | Breeding Effects and Genetic Compositions of a Backbone Parent (Fengbazhan) of Modern indica Rice in China显示文摘Fengbazhan(FBZ)and its derived varieties have been widely cultivated in China,accounting for 22%of China’s paddy fields.Therefore,evaluating the breeding.Effects of FBZ and elucidating its genetic compositions are effective strategies for interpreting the history of modern rice breeding.Using integrative bioinformatics analysis and population validation,we found that the expression of favorable genes on chromosome 6 was important for the breeding of FBZ-derived varieties and these favorable genes were gradually optimized during the breeding process,which was consistent with the conjecture of the‘Rice Core Germplasm Breeding Theory’.For several decades,tremendous efforts have been made by Chinese scientists in rice breeding to improve grain yield,nutritional quality and environmental performance,thereby achieving substantial progress in global food security.Several rice breeding technologies have been developed,including semi-dwarf breeding,utilization of heterosis,launching the‘Super Rice Project’,development of green super rice and molecular design breeding(Qian et al,2016;Bai et al,2018;Tang and Cheng,2018;Yu et al,2022).In southern China,rice can be divided into the early,middle and late rice,and the cultivation area in southern China accounts for approximately 80%of China’s rice cultivation area.Currently,several distinct rice varieties are widely cultivated in southern China. | ZHAO Lei ZHOU Shaochuan WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong ZHOU Degui CHEN Yibo GONG Rong PAN Yangyang | 2022 | Rice science2022，29，5： | 0 |
| 13 | A Successful Transmission Probability Based AP Clustering Scheme in User-Centric Cell-Free Networks显示文摘Cell-free network is a promising architecture with numerous merits in energy efficiency and macro diversity,which is easy and flexible to integrate with other communication technologies.However,its current network topology where access points(APs)are connected to a central processing unit(CPU)to jointly serve the users,causes huge burden to the fronthaul network.To deal with this problem,in this paper,we first combine thoughts in user-centric(UC)network where users are served by selected subset of APs.Then,we propose a successful transmission probability(STP)based AP clustering scheme to reduce the fronthaul capacity requirement(FCR).By using stochastic geometry and proper approximation methods,the approximated STP calculation expression is derived.Numerical simulations demonstrate that the obtained STP expression can provide a tight approximation compared to Monte Carlo simulation results under different system parameters while keeping the computation tractable.Furthermore,the relationship between the FCR and the STP threshold is formulated as a clustering optimization problem,which gives insights on clustering design in UC-CF network systems.We show by simulation results that the proposed scheme requires less fronthaul capacity than the original CF approach while ensuring the STP performance. | Xiaokang Zhou Huiyun Xia Shaochuan Wu | 2022 | China Communications2022，19，12： | 0 |
| 14 | Stay-Green Panicle Branches Improve Processing Quality of Elite Rice Cultivars显示文摘Rice panicle branches are key routes for transportation of photo synthetic products to grains.Branch senescence is an important trait that the breeders considered when breeding elite rice cultivars in South China.However,little is known about the mechanisms underlying the contributions of stay-green panicle branches to rice yield and quality.Here,we reported stay-green branches that continuously maintained transportation function and provided carbohydrates for basal grain development at the late stages of rice development.This ensured the coordination of development between apical and basal grains,and further improved the processing quality of elite rice.These findings provided strategies for improving the fullness and quality of rice grains and presented an important basis for cultivating elite rice. | CHEN Yibo ZHAO Lei WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong ZHOU Degui PAN Yangyang GONG Rong ZHOU Shaochuan | 2023 | Rice science2023，30，1： | 0 |
| 15 | Chemical Weeding in Dry Direct Seeding Fields of Single Cropping Middlelate Rice显示文摘Chemical weeding in dry direct seeding fields of single cropping middle-late rice was studied in Huida vegetable farm of Huizhou City in 2012. The main treatment was herbicide( pretilachlor + bensulfuron-methyl,Yangguo and butachlor),and the sub-treatment was application method( soil treatments,seedling treatment and integrated treatment). The results showed that 80 g pretilachlor + bensulfuron-methyl( 36% pretilachlor + 4% bensulfuron-methyl) diluted with 50 kg water could be sprayed or 200 g Yangguo( 23. 9% butachlor + 1. 1% bensulfuron-methyl) mixed with 15 kg sandy soil could be broadcasted per 667 m2 on the sowing day or the second day under moist condition of soil,which could effectively control weeds in dry direct seeding fields of single cropping middle-late rice. | Liu Huaizhen Li Kanghuo Huang Qing Lu Xiuming Zhang Bin Li Huifen Zou Jixiang Zhou Shaochuan | 2014 | Plant Diseases and Pests2014，5，3： | 0 |
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