GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development

查看全文 作　　者：Huihui [1,2,3]Zhang;Menglong [1,2]Wang;Yiqi [1,2]Li;Wei [1,2]Yan;Zhenyi [1,2]Chang;Haoling [1]Ni;Zhufeng [2]Chen;Jianxin [1]Wu;Chunjue [2]Xu;Xing Wang [2,4]Deng;Xiaoyan [1,2]Tang

机构地区：[1]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou 510631,China;[2]Shenzhen Institute of Molecular Crop Design,Shenzhen 518107,China;[3]School of Life Sciences,Capital Normal University,Beijing 100048,China;[4]School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China

出　　处：《Journal of Integrative Plant Biology》索引2020年第62卷第10期,共20页

基　　金：We thank the Microscope Center in Life Science Schoolof Sun Yat-sen University for using their facilities formicroscopic analysis,and Yao-Guang Liu for the CRISPR/Cas9 system;This work was supported by grants from Major Program of Guangdong Basic and Applied Re-search(grant number 2019B030302006);National Nat-ural Science Foundation of China(grant numbersU1901203 and 31801344);Natural Science Foundation ofGuangdong Province(grant number 2018B030308008);Shenzhen Commission on Innovation and Technology Programs(grant number JCYJ20180507181837997);Guangzhou Science and Technology lnnovation Com-mission(grant number 201804010034). 摘　　要：Pollen exine contains complex biopolymers of aliphatic lipids and phenolics.Abnormal development of pollen exine often leads to plant sterility.Molecular mechanisms regulating exine formation have been studied extensively but remain ambiguous.Here we report the analyses of three GDSL esterase/lipase protein genes,OsGELP34,OsGELP110,and OsGELP115,for rice exine for-mation.OsGELP34 was identified by cloning of a male sterile mutant gene.OsGELP34 encodes an endoplasmic reticulum protein and was mainly expressed in anthers during pollen exine formation.osgelp34 mutant displayed abnormal exine and altered expression of a number of key genes required for pollen development.OsGELP110 was previously identified as a gene differentially expressed in meiotic anthers.OsGELP110 was most homologous to OsGELP115,and the two genes showed similar gene expression patterns.Both OsGELP110 and OsGELP115 proteins were localized in peroxisomes.Individual knockout of OsGELP110 and OsGELP115 did not affect the plant fertility,but double knockout of both genes altered the exine structure and rendered the plant male sterile.OsGELP34 is distant from OsGELP110 and OsGELP115 in sequence,and osgelp34 and osgelp110/osgelp115 mutants were different in anther morphology despite both were male sterile.These results suggested that OsGELP34 and OsGELP110/OsGELP115 catalyze different compounds for pollen exine development. 关键词：development. STERILE RENDER

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GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development

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