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| 1 | Development of japonica Photo-Sensitive Genic Male Sterile Rice Lines by Editing Carbon Starved Anther Using CRISPR/Cas9显示文摘Rice is one of the most important crops as it supports over25%of total caloric intake for humans(Kusano et al.,2015).The world population reached 7.3 billion in 2015 and is projected to reach 8.5 billion in 2030(Word Population Prospects:2015 Revision).To meet the food demands for the everincreasing population with limited arable land,scarce | Quanlin Li Dabing Zhang Mingjiao Chen Wanqi Liang Jiaojun Wei Yiping Qi Zheng Yuan | 2016 | Journal of Genetics and Genomics2016，43，6： | 26 |
| 2 | 外来医疗器械的规范化管理显示文摘目的:规范外来医疗器械在医院内的管理,保证清洗、消毒、灭菌质量。方法 :依据现行标准,采用书面调查和实地调研的方式了解外来医疗器械在医院中的使用、负责处理部门或单位。结果 :87.7%的医院在使用外来医疗器械。其中由本院消毒供应中心(CSSD)负责处理外来医疗器械的医院占78.1%。结论:医院应细化外来医疗器械的管理规定,规范处理流程和加强人员培训等,以保障手术质量和患者安全。 | 张宇 巩玉秀 | 2014 | 中国护理管理2014，14，4： | 24 |
| 3 | Impact of Taurine on the proliferation and apoptosis of human cervical carcinoma cells and its mechanism显示文摘Background:Cervical cancer has the fourth highest incidence and mortality rate of all cancers in women worldwide;让seriously harms their physical and mental health.The aim of this study was to observe the roles and preliminary mechanism of Taurine(Tau)-induced apoptosis in cervical cancer cells.Methods:Cells from the human cervical cancer cell line SiHa were transfected with the recombinant plasmid pEGFP-N1-MST1(mammalian sterile 20-like kinase 1);then,the cell proliferation activity was analyzed by the MTT assay,cell apoptosis by flow cytometry,and the related protein levels by Western blotting.Results:Tau inhibited the proliferation of SiHa cells and induced apoptosis in these cells(the apoptotic rate was 21.95%in the Tau 160 mmol/L group and 30%in the Tau 320 mmol/L group),upregulated the expression of the MST1(control,0.53;Tau 40-320 mmol/L groups,0.84-1.45)and Bax(control,0.45;Tau 40-320 mmol/L groups,0.64-1.51)proteins(P<0.01),and downregulated the expression of Bcl-2(control,1.28,Tau 40-320 mmol/L groups,0.93-0.47)(P<0.01).The overexpression of MST1 promoted the apoptosis of SiHa cells,enhanced the apoptosis-inductive effects of Tau(P<0.01),upregulated the expression of the proapoptotic proteins p73,p53,PUMA(p53 upregulated modulator of apoptosis),and caspase-3,and promoted the phosphorylation of YAP(Yes-associated protein).Conclusions:Tau inhibited the proliferation and induced the apoptosis of cervical cancer SiHa cells.The MST1 protein plays an important role in the Tau-induced apoptosis of cervical cancer cells. | Hua Li Wen-Jing Ruan Li-Qiao Liu Hui-Fang Wan Xiao-Hong Yang Wei-Feng Zhu Le-Han Yu Xia-Li Zhang Fu-Sheng Wan | 2019 | Chinese Medical Journal2019，，8： | 18 |
| 4 | Wheat breeding in northern China: Achievements and technical advances显示文摘Common wheat is the major cereal crop that underpins the food safety of China. Both winter wheat and spring wheat are grown on ~24 million ha. This review aims to summarize the current status of wheat production and breeding progress in the northern wheat production areas of the country, and to review recently advanced technologies being applied in wheat breeding, including the use of dwarf-male-sterile(DMS) wheat, speed breeding and specialized wheat breeding SNP chips. Crossing is the initial step in most breeding programs. DMS wheat is a convenient tool for large scale production of hybrid seed. Speed breeding or accelerated generation turnover attempts to reduce the time taken in cultivar development. Several different SNP chips are high-throughput, genome-wide genotyping platforms for breeding and research. | Hongjie Li Yang Zhou Wenli Xin Yiqin Wei Junling Zhang Lilei Guo | 2019 | The Crop Journal2019，7，6： | 16 |
| 5 | pms3 is the locus causing the original photoperiod-sensitive male sterility mutation of‘Nongken 58S'显示文摘Photoperiod-sensitive genic male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonica cultivar ’Nongken 58’. pms3 on chromosome 12 was determined to be the locus where the original PSGMS mutation occurred, changing the normal cultivar Nongken 58 to PSGMS Nongken 58S. Large amounts of RAPD and AFLP analyses were also conducted for the fine mapping of the pms3 genomic region, which resulted in 4 molecular markers linked to pms3. Although these markers somewhat increased the marker density of this region, the pms3 locus is still located in a marker-sparse region. | 梅明华 陈亮 章志宏 李子银 徐才国 张启发 | 1999 | Science China(Life Sciences)1999，42，3： | 14 |
| 6 | Developing disease-resistant thermosensitive male sterile rice by multiplex gene editing显示文摘High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding.We introduced specific mutations into the TMS5,Pi21,and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system.We found that the transgenefree homozygous triple tms5/pi21/xa13 mutants obtained in the T1 generation displayed characteristics of thermosensitive genic male sterility(TGMS)with enhancedresistance to rice blast and bacterial blight.Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing,which could significantly accelerate the breeding of sterile lines. | Sanfeng Li Lan Shen Ping Hu Qing Liu Xudong Zhu Qian Qian Kejian Wang Yuexing Wang | 2019 | Journal of Integrative Plant Biology2019，61，12： | 11 |
| 7 | Identification of a Cytoplasmic Male Sterile Line NEA in Brassica napus L.and Its Genetic Studies显示文摘Male sterile NEA plants were identified in progenies of the radiated 92P×Aggregate-silique in Brassica napus L. In 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The double-low male sterile lines JL-4 and JL-18 were bred through successive backcrossing of the double -low variety No. 4 and No. 18 in Brassica napus L. To NEA. Restorer lines 6720 and 6722 with significant heterosis in F1 were developed. The results from investigating the restoring-maintaining relationship and inheritance of the restorer gene indicated that JL-4 and JL-18 were different from both PolCMS and Shan 2A type,and their restorer gene was controlled by a pair of dominant genic genes. | JIANG Liang-cai, PU Xiao-bin, ZHANG Qi-xing, CHEN Fang and ZHANG Jin-fang( The Crop Institute of the Sichuan Academy of Agricultural Sciences , Chengdu 610066 , P.R. China SichuanUniversity, Chengdu 610064 , P.R. China) | 2002 | Agricultural Sciences in China2002，1，4： | 10 |
| 8 | CRISPR/Cas9-mediated multiple gene editing in Brassica oleracea var.capitata using the endogenous tRNA-processing system显示文摘Cabbage(Brassica oleracea var.capitata)is a biennial plant with strong self-incompatibility and an obligate requirement for prolonged vernalization by exposure to low temperatures to induce flowering.These characteristics significantly increase the difficulty of exploiting novel germplasm induced by physical or chemical mutagens.In this study,we report a CRISPR/Cas9 gene-editing system based on endogenous tRNA processing to induce high efficiency and inheritable mutagenesis in cabbage.Using the phytoene desaturase gene BoPDS,the S-receptor kinase gene BoSRK,and the male-sterility-associated gene BoMS1 as the target genes,multisite and multiple gene mutations were achieved using a construct with tandemly arrayed tRNA-sgRNA architecture to express multiple sgRNAs.The BoSRK3 gene mutation suppressed self-incompatibility completely,converting the self-incompatible line into a self-compatible line.In addition,the BoMS1 gene mutation produced a completely male-sterile mutant,which was highly cross compatible with its nonmutant isoline at the flowering stage as a result of a simultaneous BoSRK3 gene mutation,enabling the economic propagation of the male-sterile line through bee-mediated cross-pollination.Interestingly,higher site mutation efficiency was detected when a guide sequence was inserted into a location in the tandemly arrayed tRNA-sgRNA architecture that was distal from the upstream Pol III promoter.In addition,mutation sites were also detected in the paralogous genes of the BoPDS and BoSRK genes that had fully consistent sequences or base mismatches but beyond the“seed”region in the spacer sequence compared with the target sgRNAs.Collectively,our results demonstrate that the CRISPR/Cas9 system,coupled with an endogenous tRNA-processing system,is an efficient tool to improve cabbage traits. | Cunfa Ma Chenzeng Zhu Min Zheng Mengci Liu Dejun Zhang Baoli Liu Qinfei Li Jun Si Xuesong Ren Hongyuan Song | 2019 | Horticulture Research2019，6，1： | 8 |
| 9 | A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice显示文摘Male sterility is a prerequisite for hybrid seed production.The phytohormone gibberellin(GA)is in-volved in regulating male reproductive development,but the mechanism underlying GA homeostasis in anther development remains less understood.Here,we report the isolation and characterization of a new positive regulator of GA homeostasis,swollen anther wall 1(SAW1),for anther development in rice(Oryza sativa L.).Rice plants carrying the recessive mutant allele saw1 produces abnormal anthers with swollen anther wall and aborted pollen.Clustered regularly interspaced short palindromic repeats(CRISPR)/CRIPSR-associated protein 9-mediated knockout of SAW1 in rice generated similar male sterile plants.SAW1 encodes a novel nucleus-localizing CCCH-tandem zinc finger protein,and this protein could directly bind to the promoter region of the GA synthesis gene OsGA20ox3 to induce its anther-specific expression.In the saw1 anther,the significantly decreased OsGA20ox3 expression resulted in lower bio-active GA content,which in turn caused the lower expression of the GA-inducible anther-regulator gene OsGAMYB.Thus,our results disclose the mechanism of the SAW1-GA20ox3-GAMYB pathway in controlling rice anther development,and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding. | Bin Wang Ruiqiu Fang Faming Chen Jingluan Han Yao-Guang Liu Letian Chen Qinlong Zhu | 2020 | Journal of Integrative Plant Biology2020，62，10： | 5 |
| 10 | A PRELIMINARY REPORT ON MALE STERILITY IN RICE, Oryza sativa L.显示文摘Through panicle to panicle inspection at heading stage, six male-sterile rice plants were found in the paddy fields where several varieties were grown from 1964 to 1965 in Hunan Province. The frequency of occurrence of such male-sterile rice plants under natural conditions was estimated to be 0.13 per cent. According to the characteristics of their anthers and pollen grains, these male-sterile plants can be distinguished into three different types: (1) Pollen-free type. Two plants of this type were found to be completely malesterile. Their anthers are smaller and thinner than those of the normal plant as shown in Fig. 1. They are colourless, remain unbroken after flowering, and contain no pollen grains. | 袁隆平 | 1966 | Chinese Science Bulletin1966，24，7： | 5 |
| 11 | Lectin receptor kinase OsLecRK-S.7 is required for pollen development and male fertility显示文摘Pollen grains are covered by exine that protects the pollen from stress and facilitates pollination.Here we isolated a male sterile mutant s13283 in rice exhibiting aborted pollen with abnormal exine and defective aperture.The mutant gene encodes a novel plasma membrane-localized legume-lectin receptor kinase that we named OsLecRK-S.7.OsLecRK-S.7 was expressed at different levels in all tested tissues and throughout anther development.In vitro kinase assay showed OsLecRK-S.7 capable of autophosporylation.Mutation in s13283(E560K)and mutation of the conserved ATP binding site(K418E)both knocked out the kinase activity.Mass spectrometry showed Thr376,Ser378,Thr386,Thr403,and Thr657 to be the autophosphorylation sites.Mutation of individual autophosphorylation site affected the in vitro kinase activity to different degrees,but did not abolish the gene function in fertility complementation.oslecrk-s.7 mutant plant overexpressing OsLecRK-S.7 recovered male fertility but showed severe growth retardation with reduced number of tillers,and these phenotypes were abolished by E560K or K418E mutation.The results indicated that OsLecRK-S.7 was a key regulator of pollen development. | Xiaoqun Peng Menglong Wang Yiqi Li Wei Yan Zhenyi Chang Zhufeng Chen Chunjue Xu Chengwei Yang Xing Wang Deng Jianxin Wu Xiaoyan Tang | 2020 | Journal of Integrative Plant Biology2020，62，8： | 4 |
| 12 | Identification of late-stage pollen-specific promoters for construction of pollen-inactivation system in rice显示文摘Large-scale production of male sterile seeds can be achieved by introducing a fertility-restoration gene linked with a pollen-killer gene into a recessive male sterile mutant.We attempted to construct this system in rice by using a late-stage pollen-specific(LSP)promoter driving the expression of maizeα-amylase gene ZM-AA1.To obtain such promoters in rice,we conducted comparative RNA-seq analysis of mature pollen with meiosis anther,and compared this with the transcriptomic data of various tissues in the Rice Expression Database,resulting in 269 candidate LSP genes.Initial test of nine LSP genes showed that only the most active OsLSP3 promoter could drive ZM-AA1 to disrupt pollen.We then analyzed an additional 22 LSP genes and found 12 genes stronger than OsLSP3 in late-stage anthers.The promoters of OsLSP5 and OsLSP6 showing higher expression than OsLSP3 at stages 11 and 12 could drive ZM-AA1 to inactivate pollen,while the promoter of OsLSP4 showing higher expression at stage 12 only could not drive ZM-AA1 to disrupt pollen,suggesting that strong promoter activity at stage 11 was critical for pollen inactivation.The strong pollen-specific promoters identified in this study provided valuable tools for genetic engineering of rice male sterile system for hybrid rice production. | Menglong Wang Wei Yan Xiaoqun Peng Zhufeng Chen Chunjue Xu Jianxin Wu Xing Wang Deng Xiaoyan Tang | 2020 | Journal of Integrative Plant Biology2020，62，8： | 4 |
| 13 | GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development显示文摘Pollen exine contains complex biopolymers of aliphatic lipids and phenolics.Abnormal development of pollen exine often leads to plant sterility.Molecular mechanisms regulating exine formation have been studied extensively but remain ambiguous.Here we report the analyses of three GDSL esterase/lipase protein genes,OsGELP34,OsGELP110,and OsGELP115,for rice exine for-mation.OsGELP34 was identified by cloning of a male sterile mutant gene.OsGELP34 encodes an endoplasmic reticulum protein and was mainly expressed in anthers during pollen exine formation.osgelp34 mutant displayed abnormal exine and altered expression of a number of key genes required for pollen development.OsGELP110 was previously identified as a gene differentially expressed in meiotic anthers.OsGELP110 was most homologous to OsGELP115,and the two genes showed similar gene expression patterns.Both OsGELP110 and OsGELP115 proteins were localized in peroxisomes.Individual knockout of OsGELP110 and OsGELP115 did not affect the plant fertility,but double knockout of both genes altered the exine structure and rendered the plant male sterile.OsGELP34 is distant from OsGELP110 and OsGELP115 in sequence,and osgelp34 and osgelp110/osgelp115 mutants were different in anther morphology despite both were male sterile.These results suggested that OsGELP34 and OsGELP110/OsGELP115 catalyze different compounds for pollen exine development. | Huihui Zhang Menglong Wang Yiqi Li Wei Yan Zhenyi Chang Haoling Ni Zhufeng Chen Jianxin Wu Chunjue Xu Xing Wang Deng Xiaoyan Tang | 2020 | Journal of Integrative Plant Biology2020，62，10： | 4 |
| 14 | Pyuria in patients with Kawasaki disease显示文摘Kawasaki disease(KD) is an acute, febrile vasculitis that predominantly develops in children ≤ 5 years of age and can lead to multiple organ injuries including the kidneys. Of these injuries, pyuria is a common feature of patients with KD, occurring in 30%-80% of patients. Sterile pyuria is most common in KD patients ≤ 1 year of age. KD patients with sterile pyuria exhibit more severe inflammatory reactions and may have subclinical renal injuries. Sterile pyuria in KD is associated with mononuclear cells(not neutrophils) in the urine. Although sterile pyuria in KD was at one time thought to be due to urethritis caused by a non-specific vasculitis of the urethra, recent studies suggest that sterile pyuria in KD originates from the urethra, the kidney as a resultof mild and sub-clinical renal injuries, and/or the bladder due to cystitis. Pyuria is not always sterile in KD, but can result from a urinary tract infection(UTI). As causative pathogens, Escherichia coli and Klebsiella oxytoca have been reported. The clinical phenotypes do not differ between those with or without UTI. Because some KD patients with UTIs have urinary tract abnormalities such as vesicoureteral reflux, a complete UTI workup including renal ultrasound, voiding cystourethrogram and/or dimercaptosuccinic acid renal scan recommended in KD patients with UTIs. | Toru Watanabe | 2015 | World Journal of Clinical Pediatrics2015，4，2： | 4 |
| 15 | A reporter for noninvasively monitoring gene expression and plant transformation显示文摘Reporters have been widely used to visualize gene expression,protein localization,and other cellular activities,but the commonly used reporters require special equipment,expensive chemicals,or invasive treatments.Here,we construct a new reporter RUBY that converts tyrosine to vividly red betalain,which is clearly visible to naked eyes without the need of using special equipment or chemical treatments.We show that RUBY can be used to noninvasively monitor gene expression in plants.Furthermore,we show that RUBY is an effective selection marker for transformation events in both rice and Arabidopsis.The new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions. | Yubing He Tao Zhang Hui Sun Huadong Zhan Yunde Zhao | 2020 | Horticulture Research2020，7，1： | 4 |
| 16 | The integration of transcriptomic and transgenic analyses reveals the involvement of the SA response pathway in the defense of chrysanthemum against the necrotrophic fungus Alternaria sp.显示文摘Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product uses.However,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria sp.Numerous‘Huaiju 2#’plants were inoculated with Alternaria sp.for three or five days.Metabolic analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the control.Protein activity analysis also revealed a significant increase in defense enzyme activities in infected plants.RNA-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean reads.Among these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria sp.infection,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling pathways.In particular,SA signaling was highly responsive to Alternaria sp.infection.The qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq data.One candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgenic plants to black spot.These findings indicate that the SA response pathway is likely involved in the defense of‘Huaiju 2#’against Alternaria sp.pathogens. | Xiting Zhao Lingyu Song Liwei Jiang Yuting Zhu Qinghui Gao Dandan Wang Jing Xie Meng Lv Ping Liu Mingjun Li | 2020 | Horticulture Research2020，7，1： | 3 |
| 17 | 医院落实消毒供应中心三项标准的调查分析显示文摘目的:了解医院落实消毒供应中心三项标准的情况,为医院进一步贯彻和修订标准提供科学建议。方法:采用书面调查和实地调查相结合的方式开展调查。结果:调查9省市共365所医院,其中90.9%的医院组织相关人员参与了三项标准的培训;58.9%的医院CSSD实现了集中管理;96.2%的医院依据标准将CSSD管理纳入了医院医疗质量;90%以上的医院认为,三项标准的实施对推动本院CSSD实现集中管理、提高CSSD清洗质量和消毒灭菌质量、预防医院感染发挥了较大作用。结论:医院应加强标准培训力度,进一步落实CSSD集中管理,明确CSSD的主管部门,建立CSSD人员培训制度。 | 张宇 巩玉秀 | 2014 | 中国医院2014，18，4： | 3 |
| 18 | A putative bHLH transcription factor is a candidate gene for male sterile 32,a locus affecting pollen and tapetum development in tomato显示文摘The tomato(Solanum lycopersicum)male sterile 32(ms32)mutant has been used in hybrid seed breeding programs largely because it produces no pollen and has exserted stigmas.In this study,histological examination of anthers revealed dysfunctional pollen and tapetum development in the ms32 mutant.The ms32 locus was fine mapped to a 28.5 kb interval that encoded four putative genes.Solyc01g081100,a homolog of Arabidopsis bHLH10/89/90 and rice EAT1,was proposed to be the candidate gene of MS32 because it contained a single nucleotide polymorphism(SNP)that led to the formation of a premature stop codon.A codominant derived cleaved amplified polymorphic sequence(dCAPS)marker,MS32D,was developed based on the SNP.Real-time quantitative reverse-transcription PCR showed that most of the genes,which were proposed to be involved in pollen and tapetum development in tomato,were downregulated in the ms32 mutant.These findings may aid in marker-assisted selection of ms32 in hybrid breeding programs and facilitate studies on the regulatory mechanisms of pollen and tapetum development in tomato. | Xiaoyan Liu Mengxia Yang Xiaolin Liu Kai Wei Xue Cao Xiaotian Wang Xiaoxuan Wang Yanmei Guo Yongchen Du Junming Li Lei Liu Jinshuai Shu Yong Qin Zejun Huang | 2019 | Horticulture Research2019，6，1： | 3 |
| 19 | Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon显示文摘Although male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production,its underlying molecular mechanisms in Cucurbitaceae species are still largely unclear.Here,a spontaneous male-sterile watermelon mutant,Se18,was reported to have abnormal tapetum development,which resulted in completely aborted pollen grains.Map-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1(ClATM1)encodes a basic helix-loop-helix(bHLH)transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional(BIF)domain in Se18 plants.qRT–PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6–8a of anther development.The genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated mutagenesis.Moreover,ClATM1 was significantly downregulated in the Se18 mutant,displaying a clear dose effect at the transcriptional level.Subsequent dual-luciferase reporter,β-glucuronidase(GUS)activity,and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter binding.Collectively,ClATM1 is the first male sterility gene cloned from watermelon,and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants. | Ruimin Zhang Jingjing Chang Jiayue Li Guangpu Lan Changqing Xuan Hao Li Jianxiang Ma Yong Zhang Jianqiang Yang Shujuan Tian Li Yuan Xian Zhang Chunhua Wei | 2021 | Horticulture Research2021，8，1： | 3 |
| 20 | Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers显示文摘The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation.Two populations were analyzed using cDNA-RAPD. The results showed that: ( ⅰ ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (ⅱ) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. ( ⅲ ) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated. | JIANG Shuye, CHEN Qifeng and FANG Xuanjun Biotechnology Research Center, Chinese Academy of Agricultural Science, Beijing 100081, China Institute of Genetics and Crop Breeding, Fujian Agricultural University, Fuzhou 350002, China Corresponding author | 1999 | Chinese Science Bulletin1999，44，4： | 3 |
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