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| 1 | An alternative therapy for drug-resistant epilepsy: transcutaneous auricular vagus nerve stimulation显示文摘 | Rong Peijing Liu Aihua Zhang Jianguo Wang Yuping Yang Anchao Li Liang Ben Hui Li Liping Liu Rupeng He Wei Liu Huanguang Huang Feng Li Xia Wu Peng Zhu Bing | 2014 | Chinese Medical Journal2014，，2： | 20 |
| 2 | Safety Research in Traditional Chinese Medicine: Methods, Applications, and Outlook显示文摘Traditional Chinese medicine (TCM) is a medical system that has collected and summarized abundant clinical experience over its long history of more than 2000 years. However, the frequent occurrence of TCM-induced adverse reactions has hindered the modernization and internationalization of TCM, while attracting increasing attention from around the world. Unlike chemical drugs and biological agents, the difficulties involved in research on the toxicity and safety of TCM mainly include the complexity of its components and the unpredictability of drug–body interactions. Much of TCM, which has overall therapeutic effects, has the typical mechanisms of multiple components, multiple pathways, and multiple targets. While considering the gradualness and unpredictability of TCM toxicity, the ambiguity of toxicants and safe dosage, and individual differences during long-term TCM administration, we have systematically established key techniques for the toxicity assessment of TCM. These techniques mainly include TCM toxicity discovery in an early phase, based on a combination of drug toxicology genomics and metabolomics;methods to identify dose–toxicity relationships in TCM;and integrated techniques for the exploration of TCM interactions, such as fast-screening tests based on drug-metabolizing enzymes and receptor pathways. In particular, we have developed a new technical system for TCM safety evaluation using molecular toxicology, which has been validated well in research on TCM compatibility contraindication, quality control, and allergen discovery. The application of this key technical platform is introduced here in detail. This application includes model organisms, toxicant biomarkers, a magnetic suspension technique, and the application of network toxicology and computational toxicology in research on the toxicity of Fructus toosendan, Semen cassiae, Polygonum multiflorum, and Fructus psoraleae. | Yue Gao Aihua Liang Xiaohui Fan Limin Hu Feiran Hao Yubo Li | 2019 | Engineering2019，5，1： | 19 |
| 3 | Single-cell transcriptomic atlas of primate cardiopulmonary aging显示文摘Aging is a major risk factor for many diseases,especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019(COVID-19).Resolving cellular and molecular mechanisms associated with aging in higher mammals is therefore urgently needed.Here,we created young and old non-human primate single-nucleus/cell transcriptomic atlases of lung,heart and artery,the top tissues targeted by SARS-CoV-2.Analysis of cell type-specific aging-associated transcriptional changes revealed increased systemic inflammation and compromised virus defense as a hallmark of cardiopulmonary aging.With age,expression of the SARS-CoV-2 receptor angiotensin-converting enzyme 2(ACE2)was increased in the pulmonary alveolar epithelial barrier,cardiomyocytes,and vascular endothelial cells.We found that interleukin 7(IL7)accumulated in aged cardiopulmonary tissues and induced ACE2 expression in human vascular endothelial cells in an NF-κB-dependent manner.Furthermore,treatment with vitamin C blocked IL7-induced ACE2 expression.Altogether,our findings depict the first transcriptomic atlas of the aged primate cardiopulmonary system and provide vital insights into age-linked susceptibility to SARS-CoV-2,suggesting that geroprotective strategies may reduce COVID-19 severity in the elderly. | Shuai Ma Shuhui Sun Jiaming Li Yanling Fan Jing Qu Liang Sun Si Wang Yiyuan Zhang Shanshan Yang Zunpeng Liu Zeming Wu Sheng Zhang Qiaoran Wang Aihua Zheng Shuguang Duo Yang Yu Juan Carlos Izpisua Belmonte Piu Chan Qi Zhou Moshi Song Weiqi Zhang Guang-Hui Liu | 2021 | Cell Research2021，31，4： | 10 |
| 4 | Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy显示文摘An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1×107 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8h) during a 192h in-fection. | FAN XiaoJun ZHENG Bo FU YueJun SUN Yi LIANG AiHua | 2008 | Chinese Science Bulletin2008，53，12： | 7 |
| 5 | Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency syndrome显示文摘Sijunzi decoction (SJZD) is a Chinese classical formula to treat spleen qi deficiency syndrome (SQDS) and has been widely used for thousands of years.However,the quality control (QC) standards of SJZD are insufficient.Chinmedomics has been designed to discover and verify bioactive compounds of a variety of formularapidly.In this study,we used Chinmedomics to evaluate the SJZD's efficacy against SQDS to discover the potential quality-markers (q-markers) for QC.A total of 56 compounds in SJZD were characterized in vitro,and 23 compounds were discovered in vivo.A total of 58 biomarkers were related to SQDS,and SJZD can adjust a large proportion of marker metabolites to normal level and then regulate the metabolic profile to the health status.A total of 10 constituents were absorbed as effective ingredients that were associated with overall efficacy.We preliminarily determined malonyl-ginsenoside Rb2 and ginsenoside Ro as the q-markers of ginseng;dehydrotumulosic acid and dihydroxy lanostene-triene-21-acid as the q-markers of poria;glycyrrhizic acid,isoglabrolide,and glycyrrhetnic acid as the q-markers of licorice;and 2-atractylenolide as the q-marker of macrocephala.According to the discovery of the SJZD q-markers,we can establish the quality standard that is related to efficacy. | Qiqi Zhao Xin Gao Guangli Yan Aihua Zhang Hui Sun Ying Han Wenxiu Li Liang Liu Xijun Wang | 2020 | Frontiers of Medicine2020，14，3： | 7 |
| 6 | Pathological and molecular examinations of postmortem testis biopsies reveal SARS-CoV-2 infection in the testis and spermatogenesis damage in COVID-19 patients显示文摘In late December 2019,the novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which causes coronavirus disease 2019(COVID-19),was identified in Wuhan,China,and the ensuing pandemic has led to more than 50 million infected individuals and more than one million deaths by November 10,2020(http://gffzze7c39cdc9e894ca6sknkfo66b5q5b6qkn.ffgz.tsg.suse.edu.cn/).Pathologic investigations of autopsy tissue have focused primarily on the lung,heart,and kidney,whereas morphologic data on testis injury and the effects of SARS-CoV-2 infection on spermatogenesis are limited.Although two groups did not detect SARS-CoV-2 in the semen or testes of recovered COVID-19 patients,1,2 another group confirmed SARS-CoV-2 in the semen of patients.3 Therefore,it is currently unknown whether SARS-CoV-2 infection impacts spermatogenesis and male fertility.In the present study,we evaluated the effects of SARS-CoV-2 infection on spermatogenesis by examining the pathophysiology and molecular features of testes obtained from five male COVID-19 patients at autopsy. | Xixiang Ma Chuhuai Guan Rong Chen Yunyun Wang Shenglei Feng Rongshuai Wang Guoqiang Qu Sijia Zhao Fengli Wang Xiaoli Wang Dingyu Zhang Liang Liu Aihua Liao Shuiqiao Yuan | 2021 | Cellular & Molecular Immunology2021，18，2： | 6 |
| 7 | Alginate encapsulated mesoporous silica nanospheres as a sustained drug delivery system for the poorly water-soluble drug indomethacin显示文摘We applied a combination of inorganic mesoporous silica material,frequently used as drug carriers,and a natural organic polymer alginate(ALG),to establish a sustained drug delivery system for the poorly water-soluble drug Indomethacin(IND).Mesoporous silica nanospheres(MSNs)were synthesized using an organic template method and then functionalized with aminopropyl groups through postsynthesis.After drug loading into the pores of aninopropyl functionalized MSNs(AP-MSNs),IND loaded AP-MSNs(IND-AP-MSNs)were encapsulated by ALG through the ionic interaction.The effects of surface chemical groups and ALG layer on IND release were systematically studied using scanning electron microscopy(SEM),transmission electron microscopy(TEM),nitrogen adsorption,zetapotential analysis and TGA analysis.The surface structure and surface charge changes of the ALG encapsulated AP-MSNs(ALG-AP-MSNs)were also investigated.The results showed that sustained release of IND from the designed drug delivery system was mainly due to the blockage effect from the coated ALG.We believe that this combination will help designing oral sustained drug delivery systems for poorly water-soluble drugs. | Liang Hu Changshan Sun Aihua Song Di Chang Xin Zheng Yikun Gao Tongying Jiang Siling Wang | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，4： | 2 |
| 8 | Irreversible electroporation plus allogenic Vγ9Vδ2 T cells enhances antitumor effect for locally advanced pancreatic cancer patients显示文摘Immunotherapy has limited efficacy against locally advanced pancreatic cancer(LAPC)due to the presence of an immunosuppressive microenvironment(ISM).Irreversible electroporation(IRE)can not only induce immunogenic cell death,but also alleviate immunosuppression.This study aimed to investigate the antitumor efficacy of IRE plus allogeneicγδT cells in LAPC patients.A total of 62 patients who met the eligibility criteria were enrolled in this trial,then randomized into two groups(A:n=30 and B:n=32).All patients received IRE therapy and after receiving IRE,the group A patients received at least two cycles ofγδT-cell infusion as one course continuously.Group A patients had better survival than group B patients(median OS:14.5 months vs.11 months;median PFS:11 months vs.8.5 months).Moreover,the group A patients treated with multiple courses ofγδT-cell infusion had longer OS(17 months)than those who received a single course(13.5 months).IRE combined with allogeneicγδT-cell infusion is a promising strategy to enhance the antitumor efficacy in LAPC patients,yielding extended survival benefits. | Mao Lin Xiaoyan Zhang Shuzhen Liang Haihua Luo Mohammed Alnaggar Aihua Liu Zhinan Yin Jibing Chen Lizhi Niu Yong Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 9 | Potential chronic liver toxicity in rats orally administered an ethanol extract of Huangqin(Radix Scutellariae Baicalensis)显示文摘OBJECTIVE: To investigate the potential chronic liver toxicity of oral administration of ethanol extract of Huangqin(Radix Scutellariae Baicalensis)(SBE) in Wistar rats.METHODS: SBE was administered to rats by gavage for 26 weeks, at doses of 300, 1250, or 2500 mg·kg-1·d-1 respectively. The rats were euthanized at the end of 13 and 26 weeks daily oral dosing and following 4 weeks of recovery time. The changes of hematology, urinary, blood biochemistry and histomorphology were examined at each time point and focus on liver function and histological changes.RESULTS: When SBE at a dose of up to 2500 mgkg-1 d-1 was fed to male and female rats for 2··6 weeks, the liver tissue showed some inflammatory change that predominated by leukocyte infiltrationbut returned to normal after withdrawal. In addition, high-dose SBE treatment of 26 weeks in rats,glucose, electrolyte and lipid levels also have some changes. In addition, there are no other functional or organic lesions related to SBE treatment.CONCLUSIONS: Long-term and high-dose SBE may cause liver damage, however, the structural damage of the liver can be restored after the ethanol extract stopping. SBE will be well-tolerated for long-term use as a drug or health food, but in order to ensure drug safety, liver function, and serum glucose, electrolyte and lipid levels should be monitored when using SBE long term. | Yi Yan Zhao Yong Li Chunying Zhang Yushi Bin Yang Yuan Yalan Pan Chen Wang Lianmei Liang Aihua | 2018 | Journal of Traditional Chinese Medicine2018，38，2： | 1 |
| 10 | Overexpression of CXCL5 Is Associated With Poor Survival in Patients With Pancreatic Cancer显示文摘 | Aihua Li Jonathan King Aune Moro Mark D. Sugi David W. Dawson Jeffrey Kaplan Gang Li Xuyang Lu Robert M. Strieter Marie Burdick Vay Liang W. Go Howard A. Reber Guido Eibl O. Joe Hines | 2011 | The American Journal of Pathology2011，，3： | 1 |
| 11 | Overexpression of CXCL5 Is Associated With Poor Survival in Patients With Pancreatic Cancer显示文摘 | Aihua Li Jonathan King Aune Moro Mark D. Sugi David W. Dawson Jeffrey Kaplan Gang Li Xuyang Lu Robert M. Strieter Marie Burdick Vay Liang W. Go Howard A. Reber Guido Eibl O. Joe Hines | 2011 | The American Journal of Pathology2011，，3： | 1 |
| 12 | Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes显示文摘 | Jingxue Wang Zhenlang Chen Jianzhong Du Yi Sun Aihua Liang | 2005 | Plant Cell Reports2005，，9： | 1 |
| 13 | A Thl recognized peptide P277, when tandemly repeated, enhances a Th2 immune response toward effective vaccines against autoirnmune diabetes in nonobese diabetic mice显示文摘 | Liang J Aihua Z Yu W | 2008 | J Immunol2008，180，： | 1 |
| 14 | Adaptive backstepping trajectory tracking control of robotmanipulator显示文摘 | Hu Qinglei Xu Liang Zhang Aihua | 2012 | Journal of the Franklin Institute2012，349，3： | 1 |
| 15 | Fast Remaining Capacity Estimation for Lithium-ion Batteries Based on Short-time Pulse Test and Gaussian Process Regression显示文摘It remains challenging to effectively estimate the remaining capacity of the secondary lithium-ion batteries that have been widely adopted for consumer electronics,energy storage,and electric vehicles.Herein,by integrating regular real-time current short pulse tests with data-driven Gaussian process regression algorithm,an efficient battery estimation has been successfully developed and validated for batteries with capacity ranging from 100%of the state of health(SOH)to below 50%,reaching an average accuracy as high as 95%.Interestingly,the proposed pulse test strategy for battery capacity measurement could reduce test time by more than 80%compared with regular long charge/discharge tests.The short-term features of the current pulse test were selected for an optimal training process.Data at different voltage stages and state of charge(SOC)are collected and explored to find the most suitable estimation model.In particular,we explore the validity of five different machine-learning methods for estimating capacity driven by pulse features,whereas Gaussian process regression with Matern kernel performs the best,providing guidance for future exploration.The new strategy of combining short pulse tests with machine-learning algorithms could further open window for efficiently forecasting lithium-ion battery remaining capacity. | Aihua Ran Ming Cheng Shuxiao Chen Zheng Liang Zihao Zhou Guangmin Zhou Feiyu Kang Xuan Zhang Baohua Li Guodan Wei | 2023 | Energy & Environmental Materials2023，6，3： | 1 |
| 16 | Simultaneous de-termination and pharmacokinetics of five bufadienolides inrat plasma after oral administration of Chansu extract bySPE-HPLC method显示文摘 | Yan Liang Aihua Liu Song Qin | 2008 | Journal of Pharmaceutical andBiomedical Analysis2008，46，3： | 1 |
| 17 | Direct energy conversion from xylose using xylose dehydrogenase surface displayed bacteria based enzymatic biofuel cell显示文摘 | Lin Xia Bo Liang Liang Li Xiangjiang Tang Ilaria Palchetti Marco Mascini Aihua Liu | 2013 | Biosensors and Bioelectronics2013，，： | 1 |
| 18 | Clinical characteristics of patients diagnosed with COVID-19 in Beijing显示文摘This study aimed to determine the clinical characteristics of patients diagnosed with the coronavirus disease 2019(COVID-19).Clinical data of COVID-19 patients diagnosed between January 28,2020 and February 23,2020 at the Beijing You'an Hospital were summarized and analyzed.Overall,45(18 men and 27 women)patients were included in this study.The average age of patients was 58 years(range,7–94 years).Furthermore,21 patients(47%)experienced underlying chronic diseases,with another four patients(9%)having three or more chronic diseases simultaneously.The first symptoms appeared at the onset of illness onset include fever in 36 patients(80%),cough in 23 patients(51%),and expectoration in 15 patients(33%),respectively.Patientsmay experience hepatic and renal injury as well as abnormal myocardial enzymes in varying degrees.Senior patients(≥58)and accompanying chronic diseases were considered as independent predictors for developing a severe and critically ill population with increased mortality.Laboratory results regarding the NEU percentage,NLR,ALC,and C-reactive protein levels were considered significant in predicting clinically critical disease or for prognosis assessment and thus require further studies.COVID-19 may affect multiple organs of the human body.Glucocorticoid is considered effective in the treatment of patients diagnosed with severe COVID-19. | Aihua Jin Benyong Yan Wei Hua Dandan Feng Bin Xu Lianchun Liang Caiping Guo | 2020 | Biosafety and Health2020，2，2： | 1 |
| 19 | Binding of Euplotes octocarinatus centrin with target peptide melittin显示文摘Interactions between model target peptide melittin (ME) and Euplotes octocarinatus centrin (EoCen) were investigated by fluorescence spectra, circular dichroism (CD) spectra and native polyacrylamide gel electrophoresis (PAGE). In 0.1 mol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (Hepes) and 150 mmol/L NaCl at pH 7.4, EoCen and isolated short C-terminal domain of EoCen (SC-EoCen) form 1:1 peptide:protein complexes. However, no detectable signal changes can be observed while isolated N-terminal domain of EoCen (N-EoCen) or isolated long C-terminal domain of EoCen (LC-EoCen) was added into solution of ME. The interaction between EoCen and ME is specified exclusively for the short C-terminal domain of EoCen. On the basis of fluorescence titration curves, the conditional binding constants of ME with EoCen and SC-EoCen were calculated to be logKME-EoCen = 6.81±0.33 and log- KME-SC-EoCen = 6.51±0.45, respectively. | ZHAO YaQin FENG JiuYing LIANG AiHua YANG BinSheng | 2007 | Chinese Science Bulletin2007，52，23： | 1 |
| 20 | Hsp65 serves as an immunogenic cartier for a diabetogenic peptide p277 inducing anti-inflammato- ry immune response in nod mice by nasal administration 显示文摘 | Liang J Aihua Z Yu W | 2010 | Vaccine2010，28，19： | 1 |
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