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| 1 | Isolation,Screening,and Optimization of the Fermentation Conditions of Highly Cellulolytic Bacteria from the Hindgut of Holotrichia parallela Larvae(Coleoptera:Scarabaeidae)显示文摘From the hindgut contents of Holotrichia parallela larvae,207 aerobic and facultatively anaerobic cellulolytic bacterial isolates were obtained.These bacterial isolates were assigned to 21 genotypes by amplified ribosomal DNA restriction analysis(ARDRA).A partial 16 S rDNA sequence analysis and standard biochemical and physiological tests were used for the assignment of the 21 representative isolates.Our results show that the cellulolytic bacterial community is dominated by the Proteobacteria(70.05%),followed by the Actinobacteria(24.15%),the Firmicutes(4.35%),and the Bacteroidetes(1.45%).And among the cellulolytic bacteria,some isolates,Siphonobacter aquaeclarae,Cellulosimicrobium funkei,Citrobacter freundii,Pseudomonas nitroreducens,and so on,are reported to be cellulolytic for the first time in this study.Among these isolates,a novel bacterium,designated Pseudomonas sp.HP207,with the highest endoglucanase productivity was selected for further study.The production of the endoglucanase was optimized by varying various physical culture conditions using a submerged fermentation method.Under the optimized fermentation conditions,the maximum endoglucanase activity of 1.432 U mL-1 in bacterial cultures was obtained,higher than those of the most widely studied bacteria and fungi,which are the attractive candidates for the commercial producer of cellulase.And the crude endoglucanase enzyme was also highly thermostable;approximately 55% of the original activity was maintained after pretreatment at 70°C for 1 h.Thus,from the present study,the bacterium can be added up to the database of cellulolytic bacteria,the scarab gut is an attractive source for the study of novel cellulolytic microorganisms and enzymes useful for cellulose degradation. | Ping Sheng Shengwei Huang Qi Wang Ailing Wang Hongyu Zhang | 2012 | 华中昆虫研究2012，，1： | 7 |
| 2 | A flexible new three-dimensional measurement technique by projected fringe pattern显示文摘 | Ailing Tian Zhuangde Jiang Yanqun Huang | 2006 | Optics & Laser Technology2006，38，8： | 1 |
| 3 | A Modularity Analysis Method for Forum Situation Prediction显示文摘A forum is a social network that consists of posters and the following comments made by netizens. Generally speaking, forum topics are evolving over time dynamically. In this paper, based on time series analysis and matrix modularity analysis, a novel prediction method is proposed through investigating the correlating influence of three key measurements: relationship strength, pillars, and change frequency of a forum topic. The method demonstrates that there exist some macroscopic and potential laws for forum situation prediction. Extensive experiments over large many datasets show the efficiency and effectiveness of the algorithms. | QIAN Ailing QU Binbin LU Yansheng HUANG Jin | 2011 | Wuhan University Journal of Natural Sciences2011，16，2： | 1 |
| 4 | Construction of a single chain variable fragment antibody (scFv) against tetrodotoxin (TTX) and its interaction with TTX显示文摘 | Rongzhi Wang Ailing Huang Licai Liu Shuangshuang Xiang Xiufeng Li Sumei Ling Lei Wang Tun Lu Shihua Wang | 2014 | Toxicon2014，，： | 1 |
| 5 | Targeted delivery of large fusion protein into hippocampal neurons by systemic administration显示文摘 | Lixin Xiang Rumei Zhou Ailing Fu Xingran Xu Yuqi Huang Changhua Hu | 2011 | Journal of Drug Targeting2011，，8： | 1 |
| 6 | A non-ACE2 competing human single-domain antibody confers broad neutralization against SARS-CoV-2 and circulating variants显示文摘The current COVID-19 pandemic has heavily burdened the global public health system and may keep simmering for years.The frequent emergence of immune escape variants have spurred the search for prophylactic vaccines and therapeutic antibodies that confer broad protection against SARS-CoV-2 variants.Here we show that the bivalency of an affinity maturated fully human singledomain antibody(n3113.1-Fc)exhibits exquisite neutralizing potency against SARS-CoV-2 pseudovirus,and confers effective prophylactic and therapeutic protection against authentic SARS-CoV-2 in the host cell receptor angiotensin-converting enzyme 2(ACE2)humanized mice.The crystal structure of n3113 in complex with the receptor-binding domain(RBD)of SARS-CoV-2,combined with the cryo-EM structures of n3113 and spike ecto-domain,reveals that n3113 binds to the side surface of up-state RBD with no competition with ACE2.The binding of n3113 to this novel epitope stabilizes spike in up-state conformations but inhibits SARS-CoV-2 S mediated membrane fusion,expanding our recognition of neutralization by antibodies against SARS-CoV-2.Binding assay and pseudovirus neutralization assay show no evasion of recently prevalent SARS-CoV-2 lineages,including Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2)for n3113.1-Fc with Y58L mutation,demonstrating the potential of n3113.1-Fc(Y58L)as a promising candidate for clinical development to treat COVID-19. | Zhenlin Yang Yulu Wang Yujia Jin Yuanfei Zhu Yanling Wu Cheng Li Yu Kong Wenping Song Xiaolong Tian Wuqiang Zhan Ailing Huang Shanshan Zhou Shuai Xia Xiaoxu Tian Chao Peng Cuicui Chen Yibing Shi Gaowei Hu Shujuan Du Yuyan Wang Youhua Xie Shibo Jiang Lu Lu Lei Sun Yuanlin Song Tianlei Ying | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 1 |
| 7 | ITS Planning Methodology for Chinese Cities and Its Evaluation Model 显示文摘 | Huang Ailing Shen Jinsheng Guan Wei | 2006 | IEEE Intelligent Transportation Systems2006，，： | 1 |
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