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| 1 | Pseudomonas fluorescens-like bacteria from the stomach:A microbiological and molecular study显示文摘AIM:To characterize oxidase-and urease-producing bacterial isolates,grown aerobically,that originated from antral biopsies of patients suffering from acid peptic diseases.METHODS:A total of 258 antral biopsy specimens were subjected to isolation of bacteria followed by tests for oxidase and urease production,acid tolerance and aerobic growth.The selected isolates were further characterized by molecular techniques viz.amplifications for 16S rRNA using universal eubacterial and HSP60 gene specific primers.The amplicons were subjected to restriction analysis and partial sequencing.A phylogenetic tree was generated using unweighted pair group method with arithmetic mean(UPGMA) from evolutionary distance computed with bootstrap test of phylogeny.Assessment of acidity tolerance of bacteria isolated from antrum was performed using hydrochloric acid from 10-7 mol/L to 10-1 mol/L.RESULTS:Of the 258 antral biopsy specimens collected from patients,179(69.4%) were positive for urease production by rapid urease test and 31%(80/258) yielded typical Helicobacter pylori(H.pylori) after 5-7 d of incubation under a microaerophilic environment.A total of 240(93%) antral biopsies yielded homogeneous semi-translucent and small colonies after overnight incubation.The partial 16S rRNA sequences revealed that the isolates had 99% similarity with Pseudomonas species.A phylogenetic tree on the basis of 16S rRNA sequences denoted that JQ927226 and JQ927227 were likely to be related to Pseudomonas fluorescens(P.fluorescens).On the basis ofHSP60 sequences applied to the UPGMA phylogenetic tree,it was observed that isolated strains in an aerobic environment were likely to be P.fluorescens,and HSP60 sequences had more discriminatory potential rather than 16S rRNA sequences.Interestingly,this bacterium was acid tolerant for hours at low pH.Further,a total of 250(96.9%) genomic DNA samples of 258 biopsy specimens and DNA from 240 bacterial isolates were positive for the 613 bp amplicons by targeting P.fluorescens-specific conserved putative outer membrane protein gene sequences.CONCLUSION:This study indicates that bacterial isolates from antral biopsies grown aerobically were P.fluorescens,and thus acid-tolerant bacteria other than H.pylori can also colonize the stomach and may be implicated in pathogenesis/protection. | Saurabh Kumar Patel Chandra Bhan Pratap Ajay Kumar Verma Ashok Kumar Jain Vinod Kumar Dixit Gopal Nath | 2013 | World Journal of Gastroenterology2013，19，7： | 4 |
| 2 | Gallbladder perforation:A single-center experience in north India and a step-up approach for management显示文摘Background: Spontaneous gallbladder perforation(GBP) is an uncommon diagnosis. This study presented the experience of managing spontaneous GBP over nine years at a large, tertiary care university hospital in north India and investigated the outcomes and treatment strategies. Methods: A retrospective review of prospectively maintained digital database of consecutive patients was performed. All patients received medical and/or surgical treatment for spontaneous GBP in our department between January 2010 and June 2018. Results: We identifed 151 patients(81 females and 70 males) with mean age of 53 years. Most common presenting features were pain(96.7%), fever(54.3%) and jaundice(31.1%). Most common cause was gallbladder stones(84.8%) followed by common bile duct stones(30.5%), xanthogranulomatous cholecystitis(17.9%) and malignancy(11.9%). As per Niemeier classifcation, 8.6% had type 1 GBP(free perforation in peritoneal cavity), 76.2% had type 2 GBP(localized perforation) and 13.2% had type 3 GBP(cholecystoenteric fstula). About 60% of the perforations were diagnosed preoperatively. Type 1 was more common in patients with diabetes and also had the worst prognosis. Surgery was performed in 109 patients(72.2%). Seven patients(4.6%) had a postoperative morbidity of Clavien-Dindo Ⅲ or higher. There were three mortalities in patients who underwent surgery. Conclusions: High index of suspicion is required for preoperative diagnosis of GBP, especially in types 2 and 3. Laparoscopic cholecystectomy can be difficult in these patients and patients may require open or partial cholecystectomy. Early diagnosis and step-up approach for the treatment of GBP is critical. | Vivek Gupta Abhijit Chandra Vishal Gupta Ravi Patel Amit Dangi Ajay Pai | 2022 | Hepatobiliary & Pancreatic Diseases International2022，21，2： | 2 |
| 3 | Simultaneous carbon, nitrogen and phosphorous removal from municipal wastewater in a circulating fluidized bed bioreactor 显示文摘 | AJAY PATEL JESSE ZHU | 2006 | Chemosphere2006，65，： | 1 |
| 4 | Short Hairpin RNA-Induced Myostatin Gene Silencing in Caprine Myoblast Cells In Vitro显示文摘 | Ajai K. Tripathi Umed V. Ramani Amrutlal K. Patel Dharamshibhai N. Rank Chaitanya G. Joshi | 2013 | Applied Biochemistry and Biotechnology2013，，2： | 1 |
| 5 | The legal environmem and corporate valuation: Evidence from cross -border takeover 显示文摘 | David R Kuipers Darius P Miller Ajay Patel | 2009 | International Review of Economies&Finance2009，56，10： | 1 |
| 6 | Can the agency costs of debt and equity explain the changes in executive compensation during the 1990s?显示文摘 | Bryan Stephen Robert Nash and Ajay Patel | 2006 | Journal of Co rporate Finance2006，12，3： | 1 |
| 7 | Role of Phosphoinositide 3-Kinase α, Protein Kinase C, and L-Type Ca2+ Channels in Mediating the Complex Actions of Angiotensin II on Mouse Cardiac Contractility显示文摘 | Wenbin Liang Gavin Y. Oudit Mikin M. Patel Ajay M. Shah James R. Woodgett Robert G. Tsushima Michael E. Ward Peter H. Backx | 2010 | Hypertension2010，，： | 1 |
| 8 | A statistical optical model for light reflection and penetration through sand显示文摘 | Neema D L Ajay Shah Patel A N | 1987 | International Journal of Remote Sensing1987，8，8： | 1 |
| 9 | Near-fatal Panton-Valentine leukocidin-positive Staphylococcus aureus pneumonia,shock and complicated extracorporeal membrane oxygenation cannulation:A case report显示文摘BACKGROUND Panton-Valentine leukocidin(PVL)is an exotoxin secreted by Staphylococcus aureus(S.aureus),responsible for skin and soft tissue infections.As a cause of severe necrotising pneumonia,it is associated with a high mortality rate.A rare entity,the epidemiology of PVL S.aureus(PVL-SA)pneumonia as a complication of influenza coinfection,particularly in young adults,is incompletely understood.CASE SUMMARY An adolescent girl presented with haemoptysis and respiratory distress,deteriorated rapidly,with acute respiratory distress syndrome(ARDS)and profound shock requiring extensive,prolonged resuscitation,emergency critical care and venovenous extracorporeal membrane oxygenation(ECMO).Cardiac arrest and a rare complication of ECMO cannulation necessitated intra-procedure extracorporeal cardiopulmonary resuscitation,i.e.,venoarterial ECMO.Coordinated infectious disease,microbiology and Public Health England engagement identified causative agents as PVL-SA and influenza A/H3N2 from bronchial aspirates within hours.Despite further complications of critical illness,the patient made an excellent recovery with normal cognitive function.The coordinated approach of numerous multidisciplinary specialists,nursing staff,infection control,specialist cardiorespiratory support,hospital services,both adult and paediatric and Public Health are testimony to what can be achieved to save life against expectation,against the odds.The case serves as a reminder of the deadly nature of PVL-SA when associated with influenza and describes a rare complication of ECMO cannulation.CONCLUSION PVL-SA can cause severe ARDS and profound shock,with influenza infection.A timely coordinated multispecialty approach can be lifesaving. | Joshua Cuddihy Shreena Patel Nabeela Mughal Christopher Lockie Richard Trimlett Stephane Ledot Nicholas Cheshire Ajay Desai Suveer Singh | 2021 | World Journal of Critical Care Medicine2021，10，5： | 1 |
| 10 | A statistical optical model for light reflection and penetration through sand显示文摘 | D. L. Neema Ajay Shah A. N. Patel | 1987 | International Journal of Remote Sensing1987，，8： | 1 |
| 11 | Radical Resection of Periampullary Tumors in the Elderly: Evaluation of Long-term Results显示文摘 | Oliver F. Bathe David Levi Humberto Caldera Dido Franceschi Luis Raez Ajay Patel William A. Raub Jr. Pasquale Benedetto Rajender Reddy Duane Hutson Danny Sleeman Alan S. Livingstone Joe U. Levi | 2000 | World Journal of Surgery2000，，3： | 1 |
| 12 | Short Hairpin RNA-Induced Myostatin Gene Silencing in Caprine Myoblast Cells In Vitro显示文摘 | Ajai K. Tripathi Umed V. Ramani Amrutlal K. Patel Dharamshibhai N. Rank Chaitanya G. Joshi | 2013 | Applied Biochemistry and Biotechnology2013，，2： | 1 |
| 13 | Is de novo membranous nephropathy suggestive of alloimmunity in renal transplantation?A case report显示文摘BACKGROUND Post-transplant nephrotic syndrome(PTNS)in a renal allograft carries a 48%to 77%risk of graft failure at 5 years if proteinuria persists.PTNS can be due to either recurrence of native renal disease or de novo glomerular disease.Its prognosis depends upon the underlying pathophysiology.We describe a case of post-transplant membranous nephropathy(MN)that developed 3 mo after kidney transplant.The patient was properly evaluated for pathophysiology,which helped in the management of the case.CASE SUMMARY This 22-year-old patient had chronic pyelonephritis.He received a living donor kidney,and human leukocyte antigen-DR(HLA-DR)mismatching was zero.PTNS was discovered at the follow-up visit 3 mo after the transplant.Graft histopathology was suggestive of MN.In the past antibody-mediated rejection(ABMR)might have been misinterpreted as de novo MN due to the lack of technologies available to make an accurate diagnosis.Some researchers have observed that HLA-DR is present on podocytes causing an anti-DR antibody deposition and development of de novo MN.They also reported poor prognosis in their series.Here,we excluded the secondary causes of MN.Immunohistochemistry was suggestive of IgG1 deposits that favoured the diagnosis of de novo MN.The patient responded well to an increase in the dose of tacrolimus and angiotensin converting enzyme inhibitor.CONCLUSION Exposure of hidden antigens on the podocytes in allografts may have led to subepithelial antibody deposition causing de novo MN. | Prakash I Darji Himanshu A Patel Bhavya P Darji Ajay Sharma Ahmed Halaw | 2022 | World Journal of Transplantation2022，12，1： | 0 |
| 14 | Improvement of Plasma Performance with Lithium Wall Conditioning in Aditya Tokamak显示文摘Lithiumization of the vacuum vessel wall of the Aditya tokamak using a lithium rod exposed to glow discharge cleaning plasma has been done to understand its effect on plasma performance. After the Li-coating, an increment of ~100 eV in plasma electron temperature has been observed in most of the discharges compared to discharges without Li coating, and the shot reproducibility is considerably improved. Detailed studies of impurity behaviour and hydrogen recycling are made in the Li coated discharges by observing spectral lines of hydrogen, carbon, and oxygen in the visible region using optical fiber, an interference filter, and PMT based systems. A large reduction in O I signal (up to ~ 40% to 50%) and a 20% to 30% decrease of H α signal indicate significant reduction of wall recycling. Furthermore, VUV emissions from O V and Fe XV monitored by a grazing incidence monochromator also show the reduction. Lower Fe XV emission indicates the declined impurity penetration to the core plasma in the Li coated discharges. Significant increase of the particle and energy confinement times and the reduction of Z eff of the plasma certainly indicate the improved plasma parameters in the Aditya tokamak after lithium wall conditioning. | M. B. CHOWDHURI R. MANCHANDA J. GHOSH S. B. BHATT Ajai KUMAR B. K. DAS K. A. JADEJA P. A. RAIJADA Manoj KUMAR S. BANERJEE Nilam RAMAIYA Aniruddh MALI Ketan M. PATEL Vinay KUMAR P. VASU R. BHATTACHARYAY R. L. TANNA Y. Shankara JOISA P. K. ATREY C. V. S. RAO D. Chenna REDDY P. K. CHATTOPADHYAY R. JHA Y. C. SAXENA Aditya team | 2013 | Plasma Science and Technology2013，15，2： | 0 |
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