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| 1 | Distribution of the P2X2 receptor and chemical coding in ileal enteric neurons of obese male mice(ob/ob)显示文摘AIM:To investigate the colocalization,density and profile of neuronal areas of enteric neurons in the ileum of male obese mice.METHODS:The small intestinal samples of male mice in an obese group(OG)(C57BL/6J ob/ob)and a control group(CG)(+/+)were used.The tissues were analyzed using a double immunostaining technique for immunoreactivity(ir)of the P2X2 receptor,nitric oxide synthase(NOS),choline acetyl transferase(ChAT)and calretinin(Calr).Also,we investigated the density and profile of neuronal areas of the NOS-,ChAT-and Calrir neurons in the myenteric plexus.Myenteric neurons were labeled using an NADH-diaphorase histochemical staining method.RESULTS:The analysis demonstrated that the P2X2receptor was expressed in the cytoplasm and in the nuclear and cytoplasmic membranes only in the CG.Neuronal density values(neuron/cm2)decreased 31%(CG:6579±837;OG:4556±407)and 16.5%(CG:7796±528;OG:6513±610)in the NOS-ir and calretininir neurons in the OG,respectively(P<0.05).Density of ChAT-ir(CG:6200±310;OG:8125±749)neurons significantly increased 31%in the OG(P<0.05).Neuron size studies demonstrated that NOS,ChAT,and Calr-ir neurons did not differ significantly between the CG and OG groups.The examination of NADH-diaphorase-positive myenteric neurons revealed an overall similarity between the OG and CG.CONCLUSION:Obesity may exert its effects by promoting a decrease in P2X2 receptor expression and modifications in the density of the NOS-ir,ChAT-ir and CalR-ir myenteric neurons. | Márcia Sanae Mizuno Amanda Rabello Crisma Primavera Borelli Bárbara Tavares Schfer Mariana Póvoa Silveira Patricia Castelucci | 2014 | World Journal of Gastroenterology2014，20，38： | 3 |
| 2 | Ultrafast photonic PCR显示文摘Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental science.Ultrafast multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC testing.Here,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon coupling.We demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 min.Using this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 min.Using photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)amplification.Our simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications. | Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee | 2015 | Light(Science & Applications)2015，4，1： | 2 |
| 3 | Causes of encephalitis and differences in their clinical presentations in England: a multicentre, population-based prospective study显示文摘 | Julia Granerod Helen E Ambrose Nicholas WS Davies Jonathan P Clewley Amanda L Walsh Dilys Morgan Richard Cunningham Mark Zuckerman Ken J Mutton Tom Solomon Katherine N Ward Michael PT Lunn Sarosh R Irani Angela Vincent David WG Brown Natasha S Crowcroft | 2010 | The Lancet Infectious Diseases2010，，12： | 2 |
| 4 | Behaviors and Hygiene Habits of Complete Denture Wearers 显示文摘 | Amanda P Ingrid A Helena P | 2010 | Braz Dent J2010，21，3： | 1 |
| 5 | Global association of air pollution and heart failure: a systematic review and meta-analysis显示文摘 | Anoop SV Shah Jeremy P Langrish Harish Nair David A McAllister Amanda L Hunter Ken Donaldson David E Newby Nicholas L Mills | 2013 | The Lancet2013，，： | 1 |
| 6 | Effects of drying on flavour compounds in Australian - grown ginger ( Zingiber officinale ) 显示文摘 | John P and Amanda L | 2000 | Science of Food and Agriculture2000，80，： | 1 |
| 7 | h Relatively Low Level of Ribosome Depurination by Mutant Forms of Ricin Toxin a Chain Can Trigger Protein Synthesis Inhibition, Cell Signaling and Apoptosis in Mammalian Cells 显示文摘 | Amanda E J Cheng J S Li X P | 2012 | The InternationalJournal of'' Biochemistry & Cell Biology2012，44，12： | 1 |
| 8 | Negative affect and voluntary alcohol consumption in Wistar-Kyoto ( WKY ) and Sprague-Dawley rats 显示文摘 | Amanda MT William P Joseph Kluczynski | 1999 | Physiology Behavior1999，67，2： | 1 |
| 9 | Qnantitation of bioresmethrin ,a synthetic pyrethroid grain protectant,by enzyme immunoassay 显示文摘 | David P M Simone L E | 1993 | J Agric and Food Chemistry1993，41，： | 1 |
| 10 | Effects of drying on flavour compounds in Australian-grown ginger显示文摘 | John P Amanda L | 2000 | Zingiber officinale Science of Food and Agriculture2000，80，： | 1 |
| 11 | Analgesia for the cirrhotic patient: A literature review and recommendations显示文摘 | Jeremy P Dwyer Chatura Jayasekera Amanda Nicoll | 2014 | J Gastroenterol Hepatol2014，，7： | 1 |
| 12 | Integrated project delivery:the obstacles of implementation 显示文摘 | Amanda J Fish Julia Keen P E HBDP | 2012 | ASHRAE Transactions2012，118，1： | 1 |
| 13 | Monoand polyclonal antibodies to the organophos-phate fenitrothion (2) Antibody specificity and assay performance显示文摘 | Amanda S Hill Helenl Beasley David P McAdam John H Skerritt | 1992 | Agric Food Chem1992，40，8： | 1 |
| 14 | Global association of air pollution and heart failure: a systematic review and meta-analysis显示文摘 | Anoop SV Shah Jeremy P Langrish Harish Nair David A McAllister Amanda L Hunter Ken Donaldson David E Newby Nicholas L Mills | 2013 | The Lancet2013，，： | 1 |
| 15 | Apoptosis,chemoresistance,and breast cancer:insights from the MCF-7 cell model system显示文摘 | Rebecca S Matthew B Amanda P | 2003 | Exp Biol Med2003，228，9： | 1 |
| 16 | Amylose determination of native high amylose corn starches by differential scanning calorimetry 显示文摘 | Nathan W P Amanda L W Mark R C | 2005 | Starch/Starke2005，，57： | 1 |
| 17 | Analysis of multiple data sets reveals no association between the insulin gene variable number tandem repeat element and polycystic ovary syndrome or related traits显示文摘 | Lema H Amanda B | 2005 | J Clin Endocrinol Metab2005，90，5： | 1 |
| 18 | Preoperative Education in Cholecystectomy in the Context of a Multimodal Protocol of Perioperative Care: A Randomized, Controlled Trial显示文摘 | de Aguilar-Nascimento Jos 6 E Leal Fernando S Dantas Daniela C S Anabuki Nadia T de Souza Amanda M C Silva E Lima Ver6nica P Tanajura Guilherme H Canevari Mariana | 2013 | World journal of surgery2013，34，23： | 1 |
| 19 | Clinical transplantation of a tissue-engineered airway显示文摘 | Paolo Macchiarini Philipp Jungebluth Tetsuhiko Go M Adelaide Asnaghi Louisa E Rees Tristan A Cogan Amanda Dodson Jaume Martorell Silvia Bellini Pier Paolo Parnigotto Sally C Dickinson Anthony P Hollander Sara Mantero Maria Teresa Conconi Martin A Birchall | 2008 | The Lancet2008，，9655： | 1 |
| 20 | A secondary data analysis exami- ning the needs of graduate nurses in their transition to a new role显示文摘 | Craig P Amanda K Adrian E | 2014 | Nurs Educ in Prac2014，14，2： | 1 |
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