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| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | No association of the cytotoxic T-lymphocyte associated gene CTLA4 +49A/G polymorphisms with Crohn's disease and ulcerative colitis in Hungarian population samples显示文摘瞄准:当前的工作的目标细胞毒素的 T 淋巴细胞抗原是分析 +49A/G 的流行变体的在有 Crohn 的匈牙利病人的 4 基因(CTLA4 )?ˉs 疾病(CD ) 和 ulcerative (UC ) 。方法:有 CD 的 130 个无关的题目的一个总数并且 150 与 UC,和 170 匹配的控制是为单个核苷酸多型性(SNP ) 的 genotyped。遗传型被使用 PCR/RFLP 测试决定。结果:G 等位基因频率和 GG 遗传型的流行在 CD 组,是 38.1% 和 12.3%40.6% 和 18.6% 在 UC 病人,并且 37.4% 和 15.9% 在控制组分别地。结论:当前的学习的结果显示出 +49G SNP 的那辆马车在异质接合或不在匈牙利人口为 CD 或为 UC 在同型结合的形式授与风险任何一个。 | Lili Magyari Bernadett Faragó Judit Bene Katalin Horvatovich Lilla Lakner Márta Varga Mária Figler Beáta Gasztonyi Gyula Mózsik Béla Melegh | 2007 | World Journal of Gastroenterology2007，13，15： | 3 |
| 3 | Systems biology approaches for studying the pathogenesis of non-alcoholic fatty liver disease显示文摘Non-alcoholic fatty liver disease(NAFLD) is a progressive disease of increasing public health concern.In western populations the disease has an estimated prevalence of 20%-40%,rising to 70%-90% in obese and type Ⅱ diabetic individuals.Simplistically,NAFLD is the macroscopic accumulation of lipid in the liver,and is viewed as the hepatic manifestation of the metabolic syndrome.However,the molecular mechanisms mediating both the initial development of steatosis and its progression through non-alcoholic steatohepatitis to debilitating and potentially fatal fibrosis and cirrhosis are only partially understood.Despite increased research in this field,the development of non-invasive clinical diagnostic tools and the discovery of novel therapeutic targets has been frustratingly slow.We note that,to date,NAFLD research has been dominated by in vivo experiments in animal models and human clinical studies.Systems biology tools and novel computational simulation techniques allow the study of large-scale metabolic networks and the impact of their dysregulation on health.Here we review current systems biology tools and discuss the benefits to their application to the study of NAFLD.We propose that a systems approach utilising novel in silico modelling and simulation techniques is key to a more comprehensive,better targeted NAFLD research strategy.Such an approach will accelerate the progress of research and vital translation into clinic. | Ciarán P Fisher Andrzej M Kierzek Nick J Plant J Bernadette Moore | 2014 | World Journal of Gastroenterology2014，20，41： | 2 |
| 4 | Advanced glycation endproducts and their receptor RAGE in Alzheimer’s disease显示文摘 | Velandai Srikanth Annette Maczurek Thanh Phan Megan Steele Bernadette Westcott Damian Juskiw Gerald Münch | 2009 | Neurobiology of Aging2009，，5： | 2 |
| 5 | Management of venous thromboembolism in patients with advanced cancer: a systematic review and meta-analysis显示文摘 | Simon IR Noble Mike D Shelley Bernadette Coles Susan M Williams Andrew Wilcock Miriam J Johnson | 2008 | Lancet Oncology2008，，6： | 2 |
| 6 | Interaction of the major inflammatory bowel disease susceptibility alleles in Crohn’s disease patients显示文摘AIM:To investigate the interaction of interleukin-23 receptor(IL23R)(rs1004819 and rs2201841),autophagy-related 16-like 1(ATG16L1)(rs2241880), caspase recruitment domain-containing protein 15 (CARD15)genes,and IBD5 locus in Crohn's disease(CD) patients. METHODS:A total of 315 unrelated subjects with CD and 314 healthy controls were genotyped.Interactions and specific genotype combinations of a total of eight variants were tested.The variants of IBD5locus(IGR2198a_1 rs11739135 and IGR2096a_1 rs12521868),CARD15(R702W rs2066845 and L1007fs rs2066847),ATG16L1(rs2241880)and IL23R (rs1004819,rs2201841)genes were genotyped by PCR-RFLP,the G908R(rs2066844)in CARD15 was determined by direct sequencing. RESULTS:The association of ATG16L1 T300A with CD was confirmed[P=0.004,odds ratio(OR)=1.69, 95%CI:1.19-2.41],and both IL23R variants were found to represent significant risk for the disease(P= 0.008,OR=2.05,95%CI:1.20-3.50 for rs1004819 AA;P<0.001,OR=2.97,95%CI:1.65-5.33 for rs2201841 CC).Logistic regression analysis of pairwise interaction of the inflammatory bowel disease (IBD)loci indicated that IL23R,ATG16L1,CARD15 and IBD5(IGR2198a_1)contribute independently to disease risk.We also analysed the specific combina- tions by pair of individual ATG16L1,IL23R rs1004819, rs2201841,IGR2198a_1,IGR2096a_1 and CARD15 genotypes for disease risk influence.In almost all cases,the combined risk of susceptibility pairs was higher in patients carrying two different risk-associated gene variants together than individuals with just one polymorphism.The highest OR was found for IL23R rs2201841 homozygous genotype with combination of positive CARD15 status(P<0.001,OR=9.15,95% CI:2.05-40.74). CONCLUSION:The present study suggests a cumulative effect of individual IBD susceptibility loci. | Veronika Csngei Luca Járomi EnikSáfrány Csilla Sipeky Lili Magyari Bernadett Faragó Judit Bene Noémi Polgár Lilla Lakner Patrícia Sarlós Márta Varga Béla Melegh | 2010 | World Journal of Gastroenterology2010，16，2： | 2 |
| 7 | Human adult epidermal melanocytes cultured without chemical mitogens express the EGF receptor and respond to EGF显示文摘 | Gábor Szabad Bernadett Kormos Andor Pivarcsi Márta Széll Kornélia Kis Anna Kenderessy Szabó Attila Dobozy Lajos Kemény Zsuzsanna Bata-Cs?rg? | 2007 | Archives of Dermatological Research2007，，4： | 1 |
| 8 | Sequence of the sheep interleukin - 10 encoding Cdna显示文摘 | BERNADETTE M DUTIA P H DAVID R S | 1994 | Gene1994，149，： | 1 |
| 9 | Coordination chemistry of calixarene derivatives with lower rim functionalisation and their applications 显示文摘 | Bernadette S C Denis F D John M | 2009 | Coordination Chemistry Reviews2009，253，78： | 1 |
| 10 | An empirical investigation of the relationship between change in corporate social performance and financial performance:A stakeholder theory perspective显示文摘 | Bernadette M Ruf Krishnarnurty Muralidhar Robert M brown Jay J Janney Karen Paul | 2001 | Journal of business ethics2001，32，2： | 1 |
| 11 | Interleukin-6 induces early gamma interferon production in the infected lung but is not required gener-ation of specific immunity to mycobac-terium tubereulosis Infection显示文摘 | Bernadette M Saddners Anthony A | 2000 | Infection and Immunity2000，68，6： | 1 |
| 12 | Influence of somatic cell count and storage interval on composition and processing characteristics of milk from cows in late lactation 显示文摘 | BERNADETTE O B WILLIAM J M DAVID M D | 2001 | Australian Journal of Dairy Technology2001，56，3： | 1 |
| 13 | Molecular weight determination of hydrolyzed collagens显示文摘 | Alain D Noelle B Bernadette D Sylviane G Claude R Nicole M Claude A | | 0，，6： | 1 |
| 14 | An Empirical Investigation of the Relationship between Change in Corporate Social Performance and Financial Performance: A Stakeholder Theory Perspective显示文摘 | Bernadette M Ruf Krishnarnurty Muralidhar Robea M brown Jay J Janney Karen Paul | 2001 | Journal of Business Ethics2001，32，2： | 1 |
| 15 | Increased expression of fibronectin and the α5β1 integrin in angiogenic cerebral blood vessels of mice subject to hypobaric hypoxia 显示文摘 | Richard M Stephanie H Bernadette E | 2008 | Mol Cell Neurosci2008，38，1： | 1 |
| 16 | Modelingthe growth rate and lag time of different strains of Salmonellaenteric a and Listeria monocytogenes in ready -to -eat lettuce 显示文摘 | Anderson S S A Bernadette D G M F Donald W S | 2012 | Food Microbiology2012，30，1： | 1 |
| 17 | Solvent - free chemical funetionalization of hydrogen - terminated boron - doped diamond electrodes with diazonium salts in ionic liquids显示文摘 | GALYNA S PAOLO A BERNADETTE M | 2008 | Diamond & Related Materials2008，17，： | 1 |
| 18 | Interference of raw milk autochthonous microbiota on the performance of conventional methodologies for Listeria monocytogenes and Salmonella spp. detection显示文摘 | Luís Augusto Nero Marcos Rodrigues de Mattos Márcia de Aguiar Ferreira Barros Vanerli Beloti Bernadette Dora Gombossy de Melo Franco | 2007 | Microbiological Research2007，，5： | 1 |
| 19 | 儿童健康优先和干预措施显示文摘Wilson Were和他的同事解释了为什么全球社会应该继续投资于儿童健康.完成尚未完成的儿童生存议程,解决新出现的影响儿童健康的优先问题。 | Wilson M Were Bernadette Daelmans Zulfiqar A Bhutta Trevor Duke Rajiv Bahl Cynthia Boschi-Pinto Mark Young Eric Starbuck Maharaj K Bhan 熊祎 | 2017 | 中国医院院长2017，13，17： | 1 |
| 20 | Appointments of Outsiders to Japanese Boards: Determinants and Implications for Managers 显示文摘 | STEVEN N K BERNADETTE M | 1994 | Journal of Financial Economics1994，23，2： | 1 |
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