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| 1 | Effect of supplementation of allicin on methanogenesis and ruminal microbial flora in Dorper crossbred ewes显示文摘Background: Garlic extracts have been reported to be effective in reducing methanogenesis. Related mechanisms are not well illustrated, however, and most studies have been conducted in vitro. This study investigates the effects of supplementary allicin(AL) in sheep diet on in vivo digestibility, rumen fermentation, and shifts of microbial flora.Methods: Two experiments were conducted using Dorper × thin-tailed Han crossbred ewes. In experiment 1,eighteen ewes(60.0 ± 1.73 kg BW) were randomly assigned for 29 days to either of two dietary treatments: a basal diet or the basal diet supplemented with 2.0 g AL/head?day to investigate supplementary AL on nutrient digestibility and methane emissions. In experiment 2, six ewes(65.2 ± 2.0 kg BW) with ruminal canulas were assigned to the same two dietary treatments as in experiment 1 for 42 days to investigate supplementary AL on ruminal fermentation and microbial flora. The methane emissions were determined using an open-circuit respirometry system and microbial assessment was done by q PCR of 16 S r RNA genes.Results: Supplementary AL increased the apparent digestibility of organic matter(P < 0.001), nitrogen(P = 0.006),neutral detergent fiber(P < 0.001), and acid detergent fiber(P = 0.002). Fecal nitrogen output was reduced(P = 0.001)but urinary nitrogen output was unaffected(P = 0.691), while nitrogen retention(P = 0.077) and nitrogen retention/nitrogen intake(P = 0.077) tended to increase. Supplementary AL decreased methane emissions scaled to metabolic bodyweight by 5.95 %(P = 0.007) and to digestible organic matter intake by 8.36 %(P = 0.009). Ruminal p H was unaffected(P = 0.601) while ammonia decreased(P = 0.024) and total volatile fatty acids increased(P = 0.024) in response to supplementary AL. Supplementary AL decreased the population of methanogens(P = 0.001) and tended to decrease that of protozoans(P = 0.097), but increased the populations of F. succinogenes(P < 0.001), R. flavefaciens(P = 0.001), and B. fibrisolvens(P = 0.001).Conclusions: Supplementation of AL at 2.0 g/head?day effectively enhanced OM, N, NDF, and ADF digestibility and reduced daily methane emissions(L/kg BW0.75) in ewes, probably by decreasing the population of ruminal protozoans and methanogens. | Tao Ma Dandan Chen Yan Tu Naifeng Zhang Bingwen Si Kaidong Deng Qiyu Diao | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 9 |
| 2 | Effects of protein sources for milk replacers on growth performance and serum biochemical indexes of suckling calves显示文摘This study investigated the effects of protein sources for milk replacers on growth performance and serum biochemical indexes of suckling calves. Fifty Chinese Holstein bull calves with similar BW and age were randomly allocated to 5 groups(1 control and 4 treatments) of 10 calves in each group. Five types of milk replacers were designed to have the same level of energy and protein. The protein source for milk replacers of the control group was full milk protein(MP). The protein source of milk replacers of the 4 treatment groups was composed of MP and one vegetable protein(VP)(30 and 70% of total protein). The4 types of VP were soybean protein concentrate(SP), hydrolyzed wheat protein(WP), peanut protein concentrate(PP), and rice protein isolate(RP). Results of the experiment showed: 1) there was no significant difference on average daily gain(ADG) and feed:gain ratio(F:G) among the MP, SP and RP groups(P > 0.05), whereas the ADG and F:G of the WP and PP groups were significantly lower compared with the MP group(P < 0.05); 2) there was not a significant difference in withers height, body length and heart girth among treatment groups compared with the MP group(P > 0.05). Thereby the 4 VP milk replacers had no adverse effects on body size of calves; 3) all groups showed no significant difference in the serum contents of urea nitrogen, total protein, albumin, globulin, β-hydroxybutyrate, growth hormone, insulin-like growth factor-1, and the ratio of albumin to globulin(A:G)(P> 0.05). In conclusion, SP or RP(accounts for 70% of the total protein) as calf milk replacers could substitute MP, whereas wheat gluten and PP had a significant adverse effect on growth performance in this experiment. | Kaiwu Huang Yan Tu Bingwen Si Guishan Xu Jiangpeng Guo Feng Guo Chuntao Yang Qiyu Diao | 2015 | Animal Nutrition2015，，4： | 5 |
| 3 | Development of a Novel Kilowatt Microwave Plasma Torch Source for Atomic Emission Spectrometry显示文摘 | ZHU Dan JIN Wei YU Bingwen YING Yangwei YU Haixiang SHAN Jin YAN Yuwei XU Chen WANG Baolai JIN Qinhan | 2017 | Chemical Research in Chinese Universities2017，33，5： | 1 |
| 4 | Effect of a thin spreading solvent film on the efficiency of the hexadeeane-l-ol monolayer deposited for water evaporation retardation 显示文摘 | Wu Yan Jia Qingzhu Wang Bingwen Zheng Wei Yi Shouzhi | 2010 | Russian Chemical Bulletin2010，59，9： | 1 |
| 5 | High-Temperature Ultrafast Liquid Chromatography显示文摘 | Bingwen Yan Jianhong Zhao | 2000 | Anal Chem2000，72，： | 1 |
| 6 | Net protein and metabolizable protein requirements for maintenance and growth of early-weaned Dorper crossbred male lambs显示文摘Background: Dorper is an important breed for meat purpose and widely used in the livestock industry of the world.However, the protein requirement of Dorper crossbred has not been investigated. The current paper reports the net protein(NP) and metabolizable protein(MP) requirements of Dorper crossbred ram lambs from 20 to 35 kg BW.Methods: Thirty-five Dorper × thin-tailed Han crossbred lambs weaned at approximately 50 d of age(20.3 ± 2.15 kg of BW) were used. Seven lambs of 25 kg BW were slaughtered as the baseline animals at the start of the trial. An intermediate group of seven randomly selected lambs fed ad libitum was slaughtered at 28.6 kg BW. The remaining 21 lambs were randomly divided into three levels of dry matter intake: ad libitum or 70% or 40% of ad libitum intake. Those lambs were slaughtered when the lambs fed ad libitum reached 35 kg BW. Total body N and N retention were measured.Results: The daily NP and MP requirements for maintenance were 1.89 and 4.52 g/kg metabolic shrunk BW(SBW0.75).The partial efficiency of MP utilization for maintenance was 0.42. The NP requirement for growth ranged from 12.1 to 43.5 g/d, for the lambs gaining 100 to 350 g/d, and the partial efficiency of MP utilization for growth was 0.86.Conclusions: The NP and MP requirements for the maintenance and growth of Dorper crossbred male lambs were lower than the recommendations of American and British nutritional systems. | Tao Ma Kaidong Deng Yan Tu Naifeng Zhang Bingwen Si Guishan Xu Qiyu Diao | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 1 |
| 7 | A continuous catalytic system for biodiesel production显示文摘 | Mcneff C V Mcneff L C Yan Bingwen | 2008 | Applied Catalysis A: General2008，343，12： | 1 |
| 8 | Practice and theory of high temperature liquid chromatography显示文摘 | MCNEFF C V YAN Bingwen STOLL D R | 2007 | J SepSei2007，30，11： | 1 |
| 9 | Photographic Analysis and Optical Diagnosis of Kilowatt Microwave Plasma Torch with Air Carrier Gas显示文摘The spatiotemporal motion characteristics of the kilowatt argon microwave plasma torch with the air carrier gas(kW-AC-ArMPT)and the behavior of the plasma filaments are investigated with a digital single-lens reflex(SLR)camera and a high-speed camera.Along with the introduction of the air,both the volume of the central channel and the rotational frequency of the plasma filament are increased.Besides,the excitation temperature(Texc),rotational temperature(Trot),and density of electron number(ne)of the kW-AC-ArMPT are measured with optical diagnosis.It is clearly shown that the introduction of air contributed to the rise of Trot and ne of the plasma,which is beneficial to improving the analytical performance of the plasma.Then the detection limits of some heavy metal elements are measured by kW-AC-ArMPT,which are in the ppb range.The experimental results show that the kW-ArMPT has a high tolerance to air injection at least 1.0 L/min,which allows the direct extraction of air from the environment for analysis and therefore has the potential for online and in-situ detection of ambient air quality and industrial exhaust gases. | YU Dengjie YU Bingwen ZHANG Xuchen HUANG Shiluo YING Yangwei YAN Yuwei JIN Yining JIN Wei | 2023 | Chemical Research in Chinese Universities2023，39，6： | 0 |
| 10 | Magnetic rod-based metal-organic framework metal composite as multifunctional nanostirrer with adsorptive,peroxidase-like and catalytic properties显示文摘Although magnetic stirring is frequently used to enhance the kinetics for adsorption,chemical and biochemical reactions,the introduction of stirrers inevitably leads to the adsorption of analytes and thus interferes with the efficiency of the chemical process or reaction.In this work,magnetic Fe_(3) O_(4) nanorods with tunable length-to-diameter ratio were synthesized via a hydrothermal method and used as templates for the in-situ depositing of MIL-100(Fe) and gold nanoparticles.Such nanorod-based material can not only function as an adsorbent,nanozyme,and a heterogeneous catalyst for corresponding applications but also serve as a magnetic nanostirrer to enhance kinetics.As a proof-of-concept,the capture of bacteria pathogen,mimic-peroxidase-based colorimetric detection of hydrogen peroxide,and the catalytic reduction of selected organic pollutants were conducted using the as-synthesized Fe_(3) O_(4)@MIL-100(Fe)-Au nanostirrer with and without magnetic field.The results show that the rates of bacteria capture,mimetic enzyme reaction and catalysis were tremendously expedited.We believe this magnetic field-assisted approach holds great promise for future applications,because,not only does it eliminate the use of external magnetic stirrers and thereby decrease the risk of foreign pollution but also,is adaptable for nanoscale reaction systems where conventional stirring is not applicable due to size limitations. | Benjamin Edem Meteku Jiankun Huang Jingbin Zeng Sobia Aslam Yu Zhang Xue Zhang Bingwen Cui Cong-ying Wen Zifeng Yan | 2021 | Chinese Chemical Letters2021，32，10： | 0 |
| 11 | Combination therapy with saxagliptin and vitamin D for the preservation ofβ-cell function in adult-onset type 1 diabetes:a multi-center,randomized,controlled trial显示文摘Disease modifying therapies aiming to preserveβ-cell function in patients with adult-onset autoimmune type 1 diabetes are lacking.Here,we conducted a multi-centre,randomized,controlled trial to assess theβ-cell preservation effects of saxagliptin alone and saxagliptin combined with vitamin D as adjunctive therapies in adult-onset autoimmune type 1 diabetes.In this 3-arm trial,301 participants were randomly assigned to a 24-month course of the conventional therapy(metformin with or without insulin)or adjunctive saxagliptin or adjunctive saxagliptin plus vitamin D to the conventional therapy.The primary endpoint was the change from baseline to 24 months in the fasting C-peptide.The secondary endpoints included the area under the concentration-time curve(AUC)for C-peptide level in a 2-h mixed-meal tolerance test,glycemic control,total daily insulin use and safety,respectively.The primary endpoint was not achieved in saxagliptin plus vitamin D group(P=0.18)and saxagliptin group(P=0.26).However,compared with the conventional therapy,2-h C-peptide AUC from 24 months to baseline decreased less with saxagliptin plus vitamin D(-276 pmol/L vs.-419 pmol/L;P=0.01),and not to the same degree with saxagliptin alone(-314 pmol/L;P=0.14).Notably,for participants with higher glutamic acid decarboxylase antibody(GADA)levels,the decline ofβ-cell function was much lower in saxagliptin plus vitamin D group than in the conventional therapy group(P=0.001).Insulin dose was significantly reduced in both active treatment groups than in the conventional therapy group despite all groups having similar glycemic control.In conclusion,the combination of saxagliptin and vitamin D preserves pancreaticβ-cell function in adult-onset autoimmune type 1 diabetes,an effect especially efficacious in individuals with higher GADA levels.Our results provide evidence for a novel adjunct to insulin and metformin as potential initial treatment for adult-onset type 1 diabetes.(ClinicalTrials.gov identifier:NCT02407899). | Xiang Yan Xia Li Bingwen Liu Jiaqi Huang Yufei Xiang Yuhang Hu Xiaohan Tang Ziwei Zhang Gan Huang Zhiguo Xie Houde Zhou Zhenqi Liu Xiangbing Wang Richard David Leslie Zhiguang Zhou | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 0 |
| 12 | Finished surface morphology, microstructure and magnetic properties of selective laser melted Fe-50wt% Ni permalloy显示文摘This work focuses on the structure and magnetic properties of Fe-50wt% Ni permalloy manufactured from the pre-alloyed powder by selective laser melting (SLM). The selective laser melted (SLMed) alloys were characterized by a 3D profilometer,optical microscope, scanning electron microscope, X-ray diffraction, etc. The effects of the volume energy density of laser(LVED) on structure, and magnetic properties with coercivity ( H), remanence ( B), and power losses ( P), were evaluated and discussed systematically. The results show that the relative porosity rate and the surface roughness of the SLMed specimens decreased with the increase in LVED. Only the γ-(FeNi) phase was detected in the X-ray diffraction patterns of the SLMed permalloys fabricated from the different LVEDs. Statistical analysis of optical microscopy images indicated that the grain coarsened at higher LVED. Furthermore, the microstructure of the SLMed parts was a typical columnar structure with an oriented growth of building direction. The highest microhardness reached 198 HV. Besides, the magnetic properties including B, H, and Pof SLMed samples decreased when the LVED ranged from 33.3 to 60.0 J/mm ~3 firstly and then increased while LVED further up to 93.3 J/mm, which is related to the decrease in porosity and the increase in grain size, while the higher residual stress and microcracks presented in the samples manufactured using very high LVED. The observed evolution of magnetic properties and LVED provides a good compromise in terms of reduced porosity and crack formation for the fabrication of SLMed Fe-50 wt% Ni permalloy. The theoretical mechanism in this study can offer guidance to further investigate SLMed soft magnetic alloys. | Shuohong Gao Xingchen Yan Cheng Chang Xinliang Xie Qingkun Chu Zhaoyang Deng Bingwen Lu Min Liu Hanlin Liao Nouredine Fenineche | 2022 | Acta Metallurgica Sinica(English Letters)2022，35，9： | 0 |
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