|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization显示文摘Macrophage-mediated inflammation compromises bone repair in diabetic patients.Electrical signaling cues are known to regulate macrophage functions.However,the biological effects of electrical microenvironment from charged biomaterials on the immune response for regulating osteogenesis under diabetic conditions remain to be elucidated.Herein the endogeneous electrical microenvironment of native bone tissue was recapitulated by fabricating a ferroelectric BaTiO_(3)/poly(vinylidene fluoridetrifluoroethylene)(BTO/P(VDF-TrFE))nanocomposite membrane.In vitro,the polarized BaTiO_(3)/poly(vinylidene fluoridetrifluoroethylene)(BTO/P(VDF-TrFE))nanocomposite membranes inhibited high glucose-induced M1-type inflammation,by effecting changes in cell morphology,M1 marker expression and pro-inflammatory cytokine secretion in macrophages.This led to enhanced osteogenic differentiation of human bone marrow mesenchymal stem cells(BM-MSCs).In vivo,the biomimetic electrical microenvironment recapitulated by the polarized nanocomposite membranes switched macrophage phenotype from the pro-inflammatory(M1)into the pro-healing(M2)phenotype,which in turn enhanced bone regeneration in rats with type 2 diabetes mellitus.Mechanistic studies revealed that the biomimetic electrical microenvironment attenuated pro-inflammatory M1 macrophage polarization under hyperglycemic conditions by suppressing expression of AKT2 and IRF5 within the PI3K-AKT signaling pathway,thereby inducing favorable osteo-immunomodulatory effects.Our study thus provides fundamental insights into the biological effects of restoring the electrical microenvironment conducive for osteogenesis under DM conditions,and offers an effective strategy to design functionalized biomaterials for bone regeneration therapy in diabetic patients. | Xiaohan Dai Boon Chin Heng Yunyang Bai Fuping You Xiaowen Sun Yiping Li Zhangui Tang Mingming Xu Xuehui Zhang Xuliang Deng | 2021 | Bioactive Materials2021，6，7： | 9 |
| 2 | Generatrix shape optimization of stiffened shells for low imperfection sensitivity显示文摘According to previous studies,stiffened shells with convex hyperbolic generatrix shape are less sensitive to imperfections.In this study,the effects of generatrix shape on the performances of elastic and plastic buckling in stiffened shells are investigated.Then,a more general description of generatrix shape is proposed,which can simply be expressed as a convex B-spline curve(controlled by four key points).An optimization framework of stiffened shells with a convex B-spline generatrix is established,with optimization objective being measured in terms of nominal collapse load,which can be expressed as a weighted sum of geometrically imperfect shells.The effectiveness of the proposed framework is demonstrated by a detailed comparison of the optimum designs for the B-spline and hyperbolic generatrix shapes.The decrease of imperfection sensitivity allows for a significant weight saving,which is particularly important in the development of future heavy-lift launch vehicles. | WANG Bo HAO Peng LI Gang WANG XiaoJun TANG XiaoHan LUAN Yu | 2014 | Science China(Technological Sciences)2014，57，10： | 6 |
| 3 | Novel directions of precision oncology:circulating microbial DNA emerging in cancer-microbiome areas显示文摘Microbiome research has extended into the cancer area in the past decades.Microbes can affect oncogenesis,progression,and treatment response through various mechanisms,including direct regulation and indirect impacts.Microbiota-associated detectionmethods and agents have been developed to facilitate cancer diagnosis and therapy.Additionally,the cancermicrobiome has recently been redefined.The identification of intra-tumoral microbes and cancer-related circulating microbial DNA(cmDNA)has promoted novel research in the cancer–microbiome area.In this review,we define the human system of commensal microbes and the cancer microbiome from a brand-new perspective and emphasize the potential value of cmDNA as a promising biomarker in cancer liquid biopsy.We outline all existing studies on the relationship between cmDNA and cancer and the outlook for potential preclinical and clinical applications of cmDNA in cancer precision medicine,as well as critical problems to be overcome in this burgeoning field. | Liting You Juan Zhou Zhaodan Xin J.Spencer Hauck Feifei Na Jie Tang Xiaohan Zhou Zichen Lei Binwu Ying | 2022 | Precision Clinical Medicine2022，5，1： | 2 |
| 4 | Design of a compact C-band high brightness photoinjector for an ultra-fast electron diffraction显示文摘A compact C-band high brightness photoinjector for ultra-fast electron diffraction(UED)is under development at Tsinghua University.The C-band photoinjector,operating at 5.172 GHz,uses a 1.45-cell C-band RF gun as the electron source.The design of the RF gun has addressed the physics,beam dynamics and mechanical aspects,and based on these design studies,a prototype has been fabricated.In order to obtain optimized performance for the UED,beam dynamics simulation has been carried out using the particle tracking code Astra for the photoinjector consisting of an alternative multi-cell RF gun design and a C-band compressor downstream.The beam dynamics parameters that are optimized include the bunch length,peak current,horizontal emittance,and position of the minimum bunch length. | LIU XiaoHan TANG ChuanXiang QIAN HouJun CHEN HuaiBi HUANG WenHui | 2013 | Chinese Science Bulletin2013，58，36： | 1 |
| 5 | lncR-GAS5 upregulates the splicing factor SRSF10 to impair endothelial autophagy,leading to atherogenesis显示文摘Long noncoding RNAs(lncRNAs)play a critical role in the regulation of atherosclerosis.Here,we investigated the role of the lncRNA growth arrest-specific 5(lncR-GAS5)in atherogenesis.We found that the enforced expression of lncR-GAS5 contributed to the development of atherosclerosis,which presented as increased plaque size and reduced collagen content.Moreover,impaired autophagy was observed,as shown by a decreased LC3II/LC3I protein ratio and an elevated P62 level in lncR-GAS5-overexpressing human aortic endothelial cells.By contrast,lncR-GAS5 knockdown promoted autophagy.Moreover,serine/arginine-rich splicing factor 10(SRSF10)knockdown increased the LC3II/LC3I ratio and decreased the P62 level,thus enhancing the formation of autophagic vacuoles,autolysosomes,and autophagosomes.Mechanistically,lncR-GAS5 regulated the downstream splicing factor SRSF10 to impair autophagy in the endothelium,which was reversed by the knockdown of SRSF10.Further results revealed that overexpression of the lncR-GAS5-targeted gene miR-193-5p promoted autophagy and autophagic vacuole accumulation by repressing its direct target gene,SRSF10.Notably,miR-193-5p overexpression decreased plaque size and increased collagen content.Altogether,these findings demonstrate that lncR-GAS5 partially contributes to atherogenesis and plaque instability by impairing endothelial autophagy.In conclusion,lncR-GAS5 overexpression arrested endothelial autophagy through the miR-193-5p/SRSF10 signaling pathway.Thus,miR-193-5p/SRSF10 may serve as a novel treatment target for atherosclerosis. | Yuhua Fan Yue Zhang Hongrui Zhao Wenfeng Liu Wanqing Xu Lintong Jiang Ranchen Xu Yue Zheng Xueqing Tang Xiaohan Li Limin Zhao Xin Liu Yang Hong Yuan Lin Hui Chen Yong Zhang | 2023 | Frontiers of Medicine2023，17，2： | 1 |
| 6 | Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers显示文摘 | Jian Shu Chen Wu Yetao Wu Zhiyuan Li Sida Shao Wenhui Zhao Xing Tang Huan Yang Lijun Shen Xiaohan Zuo Weifeng Yang Yan Shi Xiaochun Chi Hongquan Zhang Ge Gao Youmin Shu Kehu Yuan Weiwu He Chao Tang Yang Zhao Hongkui Deng | 2013 | Cell2013，，5： | 1 |
| 7 | Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants显示文摘Green fl uorescent protein(GFP)has been widely used for monitoring gene expression and protein localization in diverse organisms.However,highly sensitive imaging equipment,like fl uorescence microscope,is usually required for the visualization of GFP,limitings its application to fi xed locations in samples.A reporter that can be visualized in realtime regardless the shape,size and location of the target samples will increase the fl exibility and ef fi ciency of research work.Here,we report the application of a GFP-like protein,called eYGFPuv,in both transient expression and stable transformation,in two herbaceous plant species(Arabidopsis and tobacco)and two woody plant species(poplar and citrus).We observed bright fl uorescence under UV light in all of the four plant species without any effects on plant growth or development.eYGFPuv was shown to be effective for imaging transient expression in leaf and root tissues.With a focus on in vitro transformation,we demonstrated that the transgenic events expressing 1x eYGFPuv could be easily identi fi ed visually during the callus stage and the shoot stage,enabling early and ef fi cient selection of transformants.Furthermore,whole-plant level visualization of eYGFPuv revealed its ubiquitous stability in transgenic plants.In addition,our transformation experiments showed that eYGFPuv can also be used to select transgenic plants without antibiotics.This work demonstrates the feasibility of utilizing 1x eYGFPuv in studies of gene expression and plant transformation in diverse plants. | Guoliang Yuan Haiwei Lu Dan Tang Md Mahmudul Hassan Yi Li Jin-Gui Chen Gerald A.Tuskan Xiaohan Yang | 2021 | Horticulture Research2021，8，1： | 1 |
| 8 | Associations of insulin resistance and beta-cell function with abnormal lipid profile in newly diagnosed diabetes显示文摘Background:Abnormal lipids are strong predictors of cardiovascular disease in type 1 diabetes mellitus(T1DM)and type 2 diabetes mellitus(T2DM).However,the potential associations of insulin resistance(IR)and beta-cell function(BCF)with abnormal lipids in newly diagnosed T1DM or T2DM patients are not fully understood.Methods:A cross-sectional survey of 15,928 participants was conducted.Homeostasis model assessment and postprandial C-peptide levels were used to estimate IR and BCF.A restricted cubic spline(RCS)nested in binary logistic regression was used to examine the associations of IR and BCF with abnormal lipids.Results:High triglyceride(TG),low high-density lipoprotein cholesterol,and high low-density lipoprotein cholesterol(LDL-C)accounted for 49.7%,47.8%,and 59.2%of the participants,respectively.In multivariable analysis,high IR was associated with an increased risk of high TGs(P for trend<0.001)in T1DM and is associated with an elevated risk of high TG and low HDL-C(all P for trend<0.01)in T2DM.Low BCF was not associated with risks of dyslipidemia in patients with T1DM or T2DM after adjustment for potential confounders.Conclusion:High IR had different associations with the risk of dyslipidemia in newly diagnosed T1DM and T2DM patients,suggesting that early treatment that improves IR may benefit abnormal lipid metabolism. | Xiaohan Tang Xiang Yan Houde Zhou Gan Huang Xiaohong Niu Hongwei Jiang Heng Su Xilin Yang Xia Li Zhiguang Zhou | 2022 | Chinese Medical Journal2022，135，21： | 1 |
| 9 | UltraStar:A Lightweight Simulator of Ultra-Dense LEO Satellite Constellation Networking for 6G显示文摘The mega-constellation network has gained significant attention recently due to its great potential in providing ubiquitous and high-capacity connectivity in sixth-generation(6G)wireless communication systems.However,the high dynamics of network topology and large scale of mega-constellation pose new challenges to the constellation simulation and performance evaluation.In this paper,we introduce UltraStar,a lightweight network simulator,which aims to facilitate the complicated simulation for the emerging mega-constellation of unprecedented scale.Particularly,a systematic and extensible architecture is proposed,where the joint requirement for network simulation,quantitative evaluation,data statistics and visualization is fully considered.For characterizing the network,we make lightweight abstractions of physical entities and models,which contain basic representatives of networking nodes,structures and protocol stacks.Then,to consider the high dynamics of Walker constellations,we give a two-stage topology maintenance method for constellation initialization and orbit prediction.Further,based on the discrete event simulation(DES)theory,a new set of discrete events is specifically designed for basic network processes,so as to maintain network state changes over time.Finally,taking the first-generation Starlink of 11927 low earth orbit(LEO)satellites as an example,we use UltraStar to fully evaluate its network performance for different deployment stages,such as characteristics of constellation topology,performance of end-to-end service and effects of network-wide traffic interaction.The simulation results not only demonstrate its superior performance,but also verify the effectiveness of UltraStar. | Xiaoyu Liu Ting Ma Zhixuan Tang Xiaohan Qin Haibo Zhou Xuemin(Sherman)Shen | 2023 | IEEE/CAA Journal of Automatica Sinica2023，10，3： | 1 |
| 10 | Preventing autosomal-dominant hearing loss in Bth mice with CRISPR/CasRx-based RNA editing显示文摘CRISPR/RfxCas13d(CasRx)editing system can specifically and precisely cleave single-strand RNAs,which is a promising treatment for various disorders by downregulation of related gene expression.Here,we tested this RNA-editing approach on Beethoven(Bth)mice,an animal model for human DFNA36 due to a point mutation in Tmc1.We first screened 30 sgRNAs in cell cultures and found that CasRx with sgRNA3 reduced the Tmc1^(Bth)transcript by 90.8%,and the Tmc1 wild type transcript(Tmc1^(+))by 44.3%.We then injected a newly developed AAV vector(AAV-PHP.eB)based CasRx into the inner ears of neonatal Bth mice,and we found that Tmc1^(Bth)was reduced by 70.2%in 2 weeks with few off-target effects in the whole transcriptome.Consistently,we found improved hair cell survival,rescued hair bundle degeneration,and reduced mechanoelectrical transduction current.Importantly,the hearing performance,measured in both ABR and DPOAE thresholds,was improved significantly in all ages over 8 weeks.We,therefore,have validated the CRISPR/CasRx-based RNA editing strategy in treating autosomal-dominant hearing loss,paving way for its further application in many other hereditary diseases in hearing and beyond. | Ziwen Zheng Guo Li Chong Cui Fang Wang Xiaohan Wang Zhijiao Xu Huiping Guo Yuxin Chen Honghai Tang Daqi Wang Mingqian Huang Zheng-Yi Chen Xingxu Huang Huawei Li Geng-Lin Li Xiaoxiang Hu Yilai Shu | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 1 |
| 11 | Intrinsic effects of precursor functional groups on the Na storage performance in carbon anodes显示文摘The oxygen-containing functional groups in disordered carbon anodes have been widely reported to influence the Na storage performance.However,the effect of original oxygen-containing groups in the precursors on the final structures and electrochemical performance is rarely studied.Herein,we used the anthraquinone derivatives with different oxygen-containing functional groups as precursors to make the disordered carbon anodes for Na-ion batteries(NIBs).Through comprehensive structural and electrochemical analyses,we found that the different types of functional groups in carbon precursors directly affect the cross-linking process during carbonization.The original precursors containing enough inter-chain oxygen or oxygen-containing functional groups with unsaturated bonds unattached to the ring are beneficial for the oxygen atoms to remain or cross-link in structure to result in more C–O–C group,forming nanovoids and disordered structure,which then determine the high performance of the carbon anodes in NIBs.This work highlights the importance of the type/content of functional groups in precursor and provides guidance for the future design of carbon anodes in NIBs from the perspective of precursor selection. | Xiaohan Tang Fei Xie Yaxiang Lu Zhao Chen Xiangfei Li Hong Li Xuejie Huang Liquan Chen Yuanjiang Pan Yong-Sheng Hu | 2023 | Nano Research2023，16，11： | 0 |
| 12 | Geographical distribution of Aspergillus flavus in peanut harvest period in China显示文摘In order to grasp the distribution of Aspergillus flavus in the soil of peanut production areas in China,A.flavus biomarkers were tested on 555 soil samples from 37 sampling points in 17 provinces,peanut fields in four agroecological zones(Southern area,Yangtze River Basin,Northern area,Northeast area).The results showed that(1)the cultivation amount of A.flavus per gram of soil in the Yangtze River Basin is 1.30 times that of the southern area,1.56 times that of the northern area,and 6.20 times that of the northeast area,with obvious regional characteristics.(2)In the Yangtze River basin,the change of longitude in the east-west direction has no direct impact on the cultivation amount of A.flavus per gram of soil.(3)In the east coast,the A.flavus cultivated per gram of soil increased first and then decreased with the increase of latitude from south to north.(4)A.flavus can be isolated in the soil samples above 1000 m.Field pollution is an important source of aflatoxin contamination in peanut.The study on the distribution of A.flavus in soil in China could provide theoretical support for the early warning and prevention and control measures of aflatoxin contamination in peanut. | Xiaohan Liu Jiayun Fu Mingbo Wen Haohua Gu Pingping Ji Xiaofeng Yue Xiaoqian Tang Meijuan Liang Yang Zhou Qi Zhang Peiwu Li | 2023 | Oil Crop Science2023，8，2： | 0 |
| 13 | L1 scheme on graded mesh for the linearized time fractional KdV equation with initial singularity显示文摘Numerical approximation for a linearized time fractional KdV equation with initial singularity using L1 scheme on graded mesh is considered.It is proved that the L1 scheme can attain order 2−αconvergence rate with appropriate choice of the grading parameter,whereα(0<α<1)is the order of temporal Caputo fractional derivative.A fully discrete spectral scheme is constructed combing a Petrov-Galerkin spectral method for the spatial discretization,and its stability and convergence are theoretically proved.Some numerical results are provided to verify the theoretical analysis and demonstrated the sharpness of the error analysis. | Hu Chen Xiaohan Hu Jincheng Ren Tao Sun Yifa Tang | 2019 | International Journal of Modeling, Simulation, and Scientific Computing2019，10，1： | 0 |
| 14 | Quality Evaluation of Threshing and Redrying Process Based on Analytic Hierarchy Process显示文摘Improving the stability of the homogenization process to achieve the homogeneity of tobacco products is one of important targets for the redrying industry.According to the specification for threshing and redrying process,a total of 14 indicators in three categories that affect the quality of the threshing and redrying process were selected.Using analytic hierarchy process,combined with expert experiences,a judgment matrix was constructed to conduct consistency test.The weights of indices in production were obtained.This will help in evaluating the actual production quality,finding the weak links of process and adjusting the parameters of the corresponding links in a targeted manner,thereby improving the quality of production process. | Xiaohan GUO Chaohua TANG Jing TAN Qiuguo WU Bowen XIE Zhuangyu CHEN | 2020 | Asian Agricultural Research2020，12，12： | 0 |
| 15 | Combination therapy with saxagliptin and vitamin D for the preservation ofβ-cell function in adult-onset type 1 diabetes:a multi-center,randomized,controlled trial显示文摘Disease modifying therapies aiming to preserveβ-cell function in patients with adult-onset autoimmune type 1 diabetes are lacking.Here,we conducted a multi-centre,randomized,controlled trial to assess theβ-cell preservation effects of saxagliptin alone and saxagliptin combined with vitamin D as adjunctive therapies in adult-onset autoimmune type 1 diabetes.In this 3-arm trial,301 participants were randomly assigned to a 24-month course of the conventional therapy(metformin with or without insulin)or adjunctive saxagliptin or adjunctive saxagliptin plus vitamin D to the conventional therapy.The primary endpoint was the change from baseline to 24 months in the fasting C-peptide.The secondary endpoints included the area under the concentration-time curve(AUC)for C-peptide level in a 2-h mixed-meal tolerance test,glycemic control,total daily insulin use and safety,respectively.The primary endpoint was not achieved in saxagliptin plus vitamin D group(P=0.18)and saxagliptin group(P=0.26).However,compared with the conventional therapy,2-h C-peptide AUC from 24 months to baseline decreased less with saxagliptin plus vitamin D(-276 pmol/L vs.-419 pmol/L;P=0.01),and not to the same degree with saxagliptin alone(-314 pmol/L;P=0.14).Notably,for participants with higher glutamic acid decarboxylase antibody(GADA)levels,the decline ofβ-cell function was much lower in saxagliptin plus vitamin D group than in the conventional therapy group(P=0.001).Insulin dose was significantly reduced in both active treatment groups than in the conventional therapy group despite all groups having similar glycemic control.In conclusion,the combination of saxagliptin and vitamin D preserves pancreaticβ-cell function in adult-onset autoimmune type 1 diabetes,an effect especially efficacious in individuals with higher GADA levels.Our results provide evidence for a novel adjunct to insulin and metformin as potential initial treatment for adult-onset type 1 diabetes.(ClinicalTrials.gov identifier:NCT02407899). | Xiang Yan Xia Li Bingwen Liu Jiaqi Huang Yufei Xiang Yuhang Hu Xiaohan Tang Ziwei Zhang Gan Huang Zhiguo Xie Houde Zhou Zhenqi Liu Xiangbing Wang Richard David Leslie Zhiguang Zhou | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 0 |
| 16 | Feedomics provides bidirectional omics strategies between genetics and nutrition for improved production in cattle显示文摘Increasing the efficiency and sustainability of cattle production is an effective way to produce valuable animal proteins for a growing human population.Genetics and nutrition are the 2 major research topics in selecting cattle with beneficial phenotypes and developing genetic potentials for improved performance.There is an inextricable link between genetics and nutrition,which urgently requires researchers to uncover the underlying molecular mechanisms to optimize cattle production.Feedomics integrates a range of omic techniques to reveal the mechanisms at different molecular levels related to animal production and health,which can provide novel insights into the relationships of genes and nutrition/nutrients.In this review,we summarized the applications of feedomics techniques to reveal the effect of genetic elements on the response to nutrition and investigate how nutrients affect the functional genome of cattle from the perspective of both nutrigenetics and nutrigenomics.We highlighted the roles of rumen microbiome in the interactions between host genes and nutrition.Herein,we discuss the importance of feedomics in cattle nutrition research,with a view to ensure that cattle exhibit the best production traits for human consumption from both genetic and nutritional aspects. | Xiaohan Liu Yifan Tang Jiajin Wu Jian-Xin Liu Hui-Zeng Sun | 2022 | Animal Nutrition2022，，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员