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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | Roles of ischemia and hypoxia and the molecular pathogenesis of post-bum cardiac shock显示文摘 | Huang Y S Li Z Q Yang Z C | 2003 | Bums2003，29，8： | 2 |
| 3 | Evaluating the inter-lobe clearance of twin-screw compressor by the iso-clearance contour diagram(ICCD) 显示文摘 | Fong Z H Huang F C Fang H S | 2001 | Mechanism and Machine Theory2001，36，6： | 2 |
| 4 | 甜菜碱对肥育猪生长激素脉冲释放和血清代谢的影响显示文摘用添加含量为0和0.125%甜菜碱的日粮饲喂肥育猪,试验对杂交肥育猪日粮中甜菜碱对猪的生长性能、生长激素(GH)脉冲释放及血清代谢的影响进行研究。每试验组3个重复,每个重复8头猪(4头去势公猪,4头母猪),试验在饲喂后3h内每15min采集一次血液样本,测定生长激素脉冲释放。结果表明:添加甜菜碱组平均日增重增加5.45%(P〈0.05),而平均日采食量和饲料转化率则不受影响。 | Huang Q C Xu Z R Han X Y Li W F | 2007 | 饲料博览(技术版)2007，，7： | 2 |
| 5 | Evaluation of early-stage Parkinson's disease with ^99mTc-TRODAT-1 imaging显示文摘 | Huang W S Lin S Z Lin J C | 2001 | Journal of Nuclear Medicine2001，42，9： | 1 |
| 6 | Detection of coastal saline land uses with multi-temporal landsat images in Shangyu city, China 显示文摘 | SHI Z HUANG M X WANG R C | 2002 | Environmental Management2002，30，1： | 1 |
| 7 | Spectral Characteristics of the Aggregation of α,β,γ,δ-Tetrakis(p-Sulfophenyl) Porphyrin in the Presence of Proteins 显示文摘 | Huang C Z Li Y F Li N | 1998 | Bull Chem Soc Jpn1998，71，： | 1 |
| 8 | Mechanism and significance of increased glutathione level in human hepatocellular carcinoma and liver regeneration显示文摘 | HUANG ZZ CHEN C ZENG Z | 2001 | FASEB J2001，15，1： | 1 |
| 9 | Structure and magnetic ordering of Mu 2 (M = Co, Ni) 显示文摘 | Manson J L Kmety C R Huang Q Z etal | 1998 | Chem Mater1998，10，： | 1 |
| 10 | Biomass pyrolysis in an argon/hydrogen plasma reactor 显示文摘 | Zhao Z Huang H Wu C | 2001 | Environmental Science and Technology2001，，24： | 1 |
| 11 | Assembly of methylene blue on nucleic acids template as studied by resonance light-scattering technique and determination of nucleic acids of nanogram显示文摘 | Huang C Z Li Y F Deng S Y | 1999 | Bull Chem Soc Jpn1999，72，7： | 1 |
| 12 | General method for type synthesis of lower-mobility symmetrical parallel manipulators and several novel manipulators显示文摘 | Huang Z Li Q C | 2002 | Inernational Journal of Robotics Research2002，21，2： | 1 |
| 13 | Effects of mineral additives on the β-crystalline form of isotactic polypropylene显示文摘 | Feng J C Chen M C Huang Z T | 2002 | J Appl Polym Sci2002，85，： | 1 |
| 14 | Synthesis gas over alumina-supported nickel catalysts显示文摘 | Xia B Huang H Z Xie Y C | 1999 | Materials Science and Engineering B1999，，57： | 1 |
| 15 | The electrochemical behavior of austenitic stainless steel with different degrees of sensitization in the transpassive potential region in 1 mol/L H2SO4 containing chloride 显示文摘 | Huang C A Chang Y Z and Chen S C | 2004 | Corrosion Science2004，46，： | 1 |
| 16 | Factors affecting Agrobactcrium-mediated transformation and regeneration of sweet orange and citrange 显示文摘 | Yu C H Huang S Chen C X Deng Z A Ling P and Gmitter F O J | 2002 | Plant Cell Tissue and Organ Culture2002，71，： | 1 |
| 17 | Comparisons A- mong Contemporary Glacial Isostatic Adjustment Models显示文摘 | Guo J Y Huang Z W Shum C K | 2012 | J Geodyn2012，，3： | 1 |
| 18 | Application of the cross - bridge microvascular anastomosis when no recipient vessels are available for anastomosis: 85 cases 显示文摘 | Yu Z J Zeng B F Huang Y C | 2004 | Plast Reconstr Surg2004，114，5： | 1 |
| 19 | Standard outcome measures for thymic malignancies显示文摘 | HUANG j DETTERBECK F C WANG Z | 2010 | J Thorac On col2010，5，12： | 1 |
| 20 | Inhibition of melano- genesis versus antioxidant properties of essential oil extracted from leaves of Vitex negundo Linn and chemical composition analysis by GC - MS显示文摘 | HUANG H C CHANG T Y CHANG L Z | 2012 | Molecules2012，17，4： | 1 |
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