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6篇 您的检索式：作者名="CHAI Chunyan"

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		题名	作者	年代	出处	被引量
	1	PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer显示文摘Objective:As a member of the peptidyl arginine deiminase(PAD)family,PADI3 is weakly expressed in colon cancer tissues and highly expressed in adjacent colon cancer tissues.However,the role of PADI3 in colon cancer is unclear.In this study,we investigated the function and molecular mechanism of PADI3 in colon cancer tumorigenesis.Methods:Western blot and real-time PCR were used to detect the expression levels of several genes.CCK-8,flow cytometry(FCM)and colony formation assays were used to examine cell proliferation,the cell cycle and colony formation ability.RNAsequencing analysis was used to study the molecular mechanism of PADI3 in tumorigenesis.A truncation mutation experiment was performed to determine the key functional domain of PADI3.Results:PADI3 overexpression inhibited cell proliferation and colony formation and led to G1 phase arrest in both HCT116(originating from primary colon cancer)and LoVo(originating from metastatic tumor nodules of colon cancer)cells.PADI3-expressing HCT116 cells had a lower tumor formation rate and produced smaller tumors than control cells.PADI3 significantly decreased Sirtuin2(Sirt2)and Snail expression and AKT phosphorylation and increased p21 expression,and Sirt2 overexpression partly reversed the effects induced by PADI3 overexpression.Immunocytochemistry showed that PADI3 is mainly localized in the cytoplasm.Truncation mutation experiments showed that the C-domain is the key domain involved in the antitumor activity of PADI3.Conclusions:PADI3 suppresses Snail expression and AKT phosphorylation and promotes p21 expression by downregulating Sirt2 expression in the cytoplasm,and the C-domain is the key domain for its antitumor activity.	Xiaotian Chang Zhengbin Chai Jiaorui Zou Hongxing Wang Yao Wang Yabing Zheng Hui Wu Chunyan Liu	2019	Cancer Biology & Medicine2019，16，4：	4
	2	Role of nitric oxide and vascular endothelial growth factor in fluoride-induced goitrogenesis in rats显示文摘	Guoyan Liu Weidong Zhang Peng Jiang Xinhong Li Chunyan Liu Chunyan Chai	2012	Environmental Toxicology and Pharmacology2012，，2：	2
	3	Synthesis,structure and dielectric property of BaTiO3 by solid-state reaction at room temperature显示文摘	Ding Shiwen Chai Jia Feng Chunyan	2005	Chemical Journal on Internet2005，7，2：	1
	4	Fluoride causing abnormally elevated serum nitric oxide levels in chicks显示文摘	Guoyan Liu Chunyan Chai Li Cui	2003	Environmental Toxicology and Pharmacology2003，，3：	1
	5	Towards the development of a portable sensor based on a molecularly imprinted membrane for the rapid determination of salbutamol in pig urine显示文摘	Chunyan Chai Guoyan Liu Feng Li Xiaofang Liu Bing Yao Li Wang	2010	Analytica Chimica Acta2010，，2：	1
	6	Development of simple repeat(SSR)markers from a genome survey of Chinese bayberry(Myrica rubra)显示文摘	JIAO Yun JIA Huimin LI Xiongwei CHAI Mingliang JIA Huijuan CHEN Zhe WANG Guoyun CHAI Chunyan Eric van deWeg GAO Zhongshan	2012	BMC Genomics2012，13，：	1

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