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| 1 | A capacitive sensor based on molecularly imprinted polymers and poly(p-aminobenzene sulfonic acid) film for detection of pazufloxacin mesilate显示文摘A novel capacitive sensor for pazufloxacin mesilate (pazufloxacin) determination was developed by electropolymerizing p-aminobenzene sulfonic (p-ABSA) and molecularly imprinted polymers (MIPs), which was synthesized through thermal radical copolymerization of metharylic acid (MAA) and ethyl-ene glycol dimethacrylate (EGDMA) in the presence of pazufloxacin template molecules, on the gold electrode surface. Furthermore, 1-dedecanethiol was used to insulate the modified electrode. Alter-nating current (ac) impedance experiments were carried out with a Model IM6e to obtain the capaci-tance responses. Under the optimum conditions, the sensor showed linear capacitance response to pazufloxacin in the range of 5 ng·mL-1 to 5 μg·mL-1 with a relative standard deviation (RSD) 5.3% (n=7) and a detection limit of 1.8 ng·mL-1. The recoveries for different concentration levels of pazufloxacin samples varied from 94.0% to 102.0%. Electrochemical experiments indicated the capacitive sensor exhibited good sensitivity and selectivity and showed excellent parameters of regeneration and stabil-ity. | ZHOU Lu YE GuangRong YUAN Ruo CHAI YaQin CHEN SuMing | 2007 | Science China Chemistry2007，50，4： | 4 |
| 2 | Determination of carcinoembryonic antigen using a novel amperometric enzyme-electrode based on layer-by-layer assembly of gold nanoparticles and thionine显示文摘Electrochemical sensing of carcinoembryonic antigen(CEA)on a gold electrode modified by the se- quential incorporation of the mediator,thionine(Thi),and gold nanoparticles(nano-Au),through co- valent linkage and electrostatic interactions onto a self-assembled monolayer configuration is de- scribed in this paper.The enzyme,horseradish peroxidase(HRP),was employed to block the possible remaining active sites of the nano-Au monolayer,avoid the non-specific adsorption,instead of bovine serum albumin(BSA),and amplify the response of the antigen-antibody reaction.Electrochemical ex- periments indicated highly efficient electron transfer by the imbedded Thi mediator and adsorbed nano-Au.The HRP kept its activity after immobilization,and the studied electrode showed sensitive response to CEA and high stability during a long period of storage.The working range for the system was 2.5 to 80.0 ng/mL with a detection limit of 0.90 ng/mL.The model membrane system in this work is a potential biosensor for mimicking the other immunosensor and enzyme sensor. | YUAN Ruo ZHUO Ying CHAI YaQin ZHANG Ying SUN AiLi | 2007 | Science China Chemistry2007，50，1： | 3 |
| 3 | Glucose oxidase as a blocking agent-based signal amplification strategy for the fabrication of label-free amperometric immunosensors显示文摘An effective electrochemical signal amplification strategy based on enzyme membrane modification and redox probe immobilization was proposed to construct an amperometric immunosensor.L-cysteine@ferrocene functionalized chitosan,which possessed not only efficient redox-activity but also excellent film-forming ability,was coated on the bare glass carbon electrode. Moreover,the thiol groups(SH)in the ferrocenyl compound were used for gold nanoparticles immobilization via the strong bonding interaction,which could further be utilized for the immobilization of antibody biomolecules with well-retained bioactivities.Finally,glucose oxidase(GOD)as the enzyme membrane was employed to block the possible remaining active sites and avoid the nonspecific adsorption.With the excellent electrocatalytic properties of GOD towards glucose,the amplification of antigen-antibody interaction and the enhanced sensitivity could be achieved.Under the optimal conditions,the linear range of the proposed immunosensor for the determination of carcinoembryonic antigen(CEA)was from 0.05 to 100 ng/mL with a detection limit of 0.02 ng/mL(S/N=3).Moreover,the immunosensor exhibited good selectivity,stability and reproducibility, which provided a promising potential for clinical immunoassay. | SONG ZhongJu YUAN Ruo CHAI YaQin CHE Xin LV Ping | 2011 | Science China Chemistry2011，54，3： | 3 |
| 4 | Highly sensitive potentiometric immunosensor for hepatitis B surface antigen diagnosis显示文摘A highly sensitive potentiometric immunosensor for the diagnoses of epidemic dis- eases has been developed by means of self-assembly to immobilize hepatitis B surface antibody (HBsAb) for the detection of hepatitis B surface antigen (HBsAg) as a model. At first, the Nafion containing ?SO3 groups was immobilized on a platinum electrode surface to absorb the ?NH3 ? + groups of antibody molecules via the opposite-charged adsorption technique, in the meantime, hepatitis B surface antibodies were adsorbed onto the surface of Au nanoparticles, then hepatitis B surface antibodies and Au nanopartilces were entrapped into polyvinyl butyral on the surface of Nafion film. The modified procedure was further characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The influence and factors influencing the per- formance of resulting immunosensor were studied in detail. The resulting immunosensor exhib- ited sigmoid curve with log HBsAg concentrations, high sensitivity, wide linear range from 26 to 1280 ng·mL?1 with a detection limit of 3.1 ng·mL?1, rapid potentiometric response (<3 min) and long-term stability (>4 months). Analytical results of clinical samples show that the developed immunoassay is comparable with the enzyme-linked immunosorbent assays (ELISAs) method, implying a promising alternative approach for detecting HBsAg in the clinical diagnosis. | YUAN Ruo TANG Dianping CHAI Yaqin ZHANG Lingyan LIU Yan ZHONG Xia DAI Jianyuan | 2005 | Science China Chemistry2005，48，1： | 2 |
| 5 | Schiff base complexes of cobalt( Ⅱ ) as neutral carriers for highly selective iodide electrodes显示文摘 | YUAN Ruo CHAI Yaqin LIU Dong | 1993 | Anal Chem1993，65，19： | 1 |
| 6 | New type of redox nanoprobe:c60-based nanomaterial and its application in electrochemical immunoassay for doping detection显示文摘 | Han Jing Zhuo Ying Chai Yaqin | 2015 | Analytical chemistry2015，87，： | 1 |
| 7 | A novel amperometric immunosensor based on layer-by-layer assembly of gold nanoparticles-multi-walled carbon nanotubes-thionine multilayer films on polyelectrolyte surface 显示文摘 | Chaofeng Ou Ruo Yuan Yaqin Chai | 2007 | Analytica Chimica Acta2007，603，： | 1 |
| 8 | Sequence determination and anticoagulant and antithrombotic activities of a novel sulfated fucan isolated from the sea cucumber Isostichopus badionotus显示文摘 | Shiguo Chen Yaqin Hu Xinqian Ye Guoyun Li Guangli Yu Changhu Xue Wengang Chai | 2012 | BBA - General Subjects2012，，7： | 1 |
| 9 | 显示文摘 | Wang Yan Yuan Ruo Chai Yaqin | 2011 | Biosens Bioelec- tron2011，30，1： | 1 |
| 10 | Electrochemical immuno-bioanalysis for carcinoma antigen 125 based on thionine and gold nanoparticles-modified carbon paste interface显示文摘 | Dianping Tang Ruo Yuan Yaqin Chai | 2006 | Analytica Chimica Acta2006，，2： | 1 |
| 11 | Ru(bpy)32+-doped silica nanoparticles labeling for a sandwich-type electrochemilumines- cenee 1851-1855 显示文摘 | Xia Yang Ruo Yuan Yaqin Chai | 2010 | Biosensors and Bioelectronics2010，25，： | 1 |
| 12 | An amperometric immunosensor with a DNA polyion complex membrane/gold nanoparticles-backbone for antibody immobilisation显示文摘 | Na Li Hongwen Zhao Ruo Yuan Kanfu Peng Yaqin Chai | 2008 | Electrochimica Acta2008，，2： | 1 |
| 13 | Enhancement of carcinoembryonicantibody immobilization on gold electrode modified by gold nanoparticles and SiO2/Thion-ine nanocomposite 显示文摘 | Zhuo Ying Yuan Ruo Chai Yaqin | 2009 | Journal of Electroanalytical Chemistry2009，628，12： | 1 |
| 14 | Amperometric third-generation hydrogen peroxide biosensor based on the immobilization of hemoglobin on multiwall carbon nanotubes and gold colloidal nanoparticles显示文摘 | Shihong Chen Ruo Yuan Yaqin Chai Lingyan Zhang Na Wang Xuelian Li | 2006 | Biosensors and Bioelectronics2006，，7： | 1 |
| 15 | A new potentiometric immunosensor for determination of α-fetoprotein based on improved gelatin-silver complex film 显示文摘 | Zhu Qiang Ruo Yuan Yaqin Chai | 2006 | Electrochimica Acta2006，51，18： | 1 |
| 16 | Simultaneous voltammetric determination for DA, AA and NO2 based on grapheme/poly- cyclodextrin/MWCNTs nanocomposite platform 显示文摘 | Zhang Yu Yuan Ruo Chai Yaqin | 2011 | Biosensors and bioelectronics2011，26，： | 1 |
| 17 | Multilayer structured ampero- built by self-assembly of a redox multi-wall carbon nanotube composite 显示文摘 | Huilan metric Su Ruo Yuan Yaqin Chai | 2009 | Electrochimica Acta2009，54，17： | 1 |
| 18 | Highly sensitive, reagentless amperometric immunosensor based on a novel redox-active organic-inorganic composite film 显示文摘 | Zhongyuan Liu Ruo Yuan Yaqin Chai | 2008 | Sensors and Actuators B: Chemical2008，134，2： | 1 |
| 19 | Amperometric immunosensors based on layer-by-layer assembly of gold nanoparticles and methylene blue on thiourea modified glassy carbon electrode for determination of human chorionic gonadotrophin显示文摘 | CHAI Rong YUAN Ruo CHAI Yaqin | 2008 | Talanta2008，74，5： | 1 |
| 20 | Porous redox-active Cu2O- SiO2 nanostructured film: Preparation, characterization and application for a label-free amperometric ferritin immunosensor 显示文摘 | Xia Yang Ruo Yuan Yaqin Chai | 2009 | Talanta2009，78，2： | 1 |
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