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| 1 | Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition显示文摘Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions. | WANG Shanyue CUI Xueping XU Ruyi GAO Meirong SUI Liying | 2019 | Journal of Oceanology and Limnology2019，37，5： | 2 |
| 2 | The symptom difference induced by Tobacco mosaic virus and Tomato mosaic virus in tobacco plants containing the N gene is determined by movement protein gene显示文摘Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) are two closely related viruses in the genus Tobamovirus, but they induce obviously different sizes of necrotic lesions in tobacco plants containing the N gene. Comparison of the symptoms produced by TMV, ToMV and a chimaeric virus (T/OMP), in which the TMV movement protein (MP) gene was re- placed by the ToMV MP gene, showed T/OMP caused necrotic lesions that were similar in size to those of ToMV in tobacco plants containing the N gene. The coat protein and MP of the three viruses accumulated in planta with similar levels, and the replication level of TMV and T/OMP in protoplasts also had no difference. Comparison of the activities of defense-related enzymes (PAL, POD and PPO) induced by the three viruses also showed that the variability of enzyme activity induced by T/OMP was similar to that induced by TMV, but different from that induced by ToMV. The results indicate that the size difference of necrotic lesions induced by TMV and ToMV in to- bacco plants containing the N gene results from the functional difference of their MP genes. | YU Cui1, HU Dongwei1, DONG Jiahong2, CUI Xiaofeng1, WU Junjie1, YU Jialin2 & ZHOU Xueping1 1. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China 2. National Key Laboratory on Agrobiotechnology, China Agricultural University, Beijing 100094, China Correspondence should be addressed to Zhou Xueping | 2004 | Science China(Life Sciences)2004，47，6： | 2 |
| 3 | SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants显示文摘RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals.However,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in plants.Here,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide.Pelota enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all potyvirids.This allows Pelota to target the virus and act as a viral restriction factor.Furthermore,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta.Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation.These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants. | Linhao Ge Buwei Cao Rui Qiao Hongguang Cui Shaofang Li Hongying Shan Pan Gong Mingzhen Zhang Hao Li Aiming Wang Xueping Zhou Fangfang Li | 2023 | Molecular Plant2023，16，3： | 2 |
| 4 | Rolling reduction-dependent deformation mechanisms and tensile properties in aβtitanium alloy显示文摘This work investigated the dependence of deformation mechanisms and mechanical properties on cold rolling reductions of the metastable TB8 titanium alloy.Results shown that the crystal orientation of the matrix changes with the increasing level of reduction,leading to the activation of complex deformation mechanisms in the matrix.When the rolling reduction is 10%,the deformation mechanisms are dominated by dislocations and{332}<113>deformation twins.As the reductions increase to 20%-50%,the secondary deformation twinning(SDT)is triggered in primary deformation twins besides the primary kink band is activated.Meanwhile,the secondary kink bands and{332}<113>twins have be observed in the primary kink bands.When the reduction reaches to 60%,the deformation mechanisms are dominated by dislocations and deformation twins.Furthermore,the matrix refined by crisscrossing among the twins,kink bands and other deformation mechanisms during cold rolling,which shortens the dislocation mean free path and then affects the strength and shape of the alloy.The dynamic Hall-Petch effect and the interaction between multi-scale deformed structures control the work hardening behavior of the alloy. | Zhaoxin Du Qiwei He Ruirun Chen Fei Liu Jingyong Zhang Fei Yang Xueping Zhao Xiaoming Cui Jun Cheng | 2022 | Journal of Materials Science & Technology2022，，9： | 2 |
| 5 | Immersing Treatment of Steel Plate Surface in Steel-Aluminum Solid to Liquid Bonding显示文摘The interfacial status of the steel-aluminum solid to liquid bonding plates (their steel plate surfaces were or were not immersed in flux aqueous solution) were measured by using SEM (Scanning Electron Microscope) and X-ray diffraction . The results showed that the layer of flux (the minimum thickness was 15 μm on the steel plate surface) could protect the steel plate surface from oxidizing effectively at high temperature in solid to liquid bonding. The melt temperatUre of the flux should be lower than 580 ℃ so that it could be melted and removed completely. No. 1 flux (patent product made by the author) made up of halogeindes could also force liquid aluminum to infiltrate into steel plate surface and thus the interfacial shear strength of the bonding plate was rather large. | Peng Zhang Yunhui Du Xueping Ren Yonglin Kang Hanwu Liu Jianzhong Cui Limin Ba (Material Science and Engineering School, University of Science and Technique Beijing, Beijing 100083, China)(Metal Forming Department Northeastern University, Shenyang 1 | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，1： | 2 |
| 6 | Electrochemical Detection of Superoxide Anion Released by Living Cells by Manganese(Ⅲ) Tetraphenyl Porphine as Superoxide Dismutase Mimic显示文摘Superoxide anion,one of the most active reactive oxygen species,is associated with the development of many diseases.So monitoring superoxide anion in living cells is of great significance for the pathological research of many diseases.In this work,a new non-enzymatic sensor tor the detection of superoxide anion(O^·-2)was developed,which was iabricated by the nanocomposites composed of manganese(Ⅲ)tetraphenyl porphine(MnTPP)as superoxide dismutase mimic and electrochemical reduced graphene oxide(ERGO)as electrode support material to modify the glassy carbon electrode(GCE).The electrochemical behavior of the fabricated electrode(MiiTPP/ERGO/GCE)was performed by electrochemical impedance spectroscopy(EIS)and cyclic voltammetry(CV),which revealed that MnTPP/ERGO/GCE possessed good catalytic ability to theelectrochemical reduction of O^·-2.The MnTPP/ERGO/GCE showed excellent electroanalysis perfonnance towards O^·-2 using the technique of diflerential pulse voltanimetry(DPV)with a linear relationship in the range of 0.2—110.0μmol/L,a sensitivity of 445 μA· L·mmol^-1·cm^- 2 and a detection limit of 0.039 μmol/L(S/N=3).The real-time monitoring of O^·-2 from MCF-7 breast cancer cells stimulated by zymosan was realized in this work,which indicates that the MnTPP/ERGO/GCE hold potential application for electrochemical quantification of superoxide anions in biological applications. | CUI Min REN Jujie WEN Xiaofang LI Na XING Yifei ZHANG Cong HAN Yuanyuan JI Xueping | 2020 | Chemical Research in Chinese Universities2020，36，5： | 1 |
| 7 | A novel snail-inspired bionic design of titanium with strontium-substituted hydroxyapatite coating for promoting osseointegration显示文摘As the population ages,more and more people are suffering from osteoarthritis(OA),resulting in an increasing requirement for joint implants.Surface modification to improve the topology and composition of the implants has been proved to be an effective way to improve the primary stability and long-term success rate of joint implants.In this work,a bionic micro/nano-structure accompanied with a strontium-substituted hydroxyapatite(SrHA)coating was fabricated on titanium(Ti)surface via electrochemical corrosion,ultrasonic treatment,and hydrothermal deposition methods.Thein vitro study demonstrated that the bionic structure and the bioactive apatite could synergistically increase the expressions of integrin-related gene(ITGα5β1)and osteoblastic genes(Col-I and OCN),and thus promote osteoblast growth.In addition,owing to the anti-bone resorption property of Sr^(2+),the coating could effectively inhibit osteoclast differentiation and proliferation.In a word,the prepared samples not only promoted osteogenesis but also inhibited osteoclastogenesis.The in vivo experiment via a rabbit model found that the bionic structured surface provided the pore for new bone ingrowth,which was beneficial to the mechanical interlocking between the implant and bone.Moreover,the bionic structure and bioactive SrHA coating had a synergistic effect on promoting bone formation,osseointegration,and bone-implant bonding strength.This study therefore presented a new strategy to fabricate bio-functionalized Ti-based implants for potential application in orthopedics field. | Zhen Geng Xueping Li Luli Ji Zhaoyang Li Zhenduo Cui Jing Wang Jingyuan Cui Xianjin Yang Changsheng Liu | 2021 | Journal of Materials Science & Technology2021，，20： | 0 |
| 8 | Advances in controlled-release fertilizer encapsulated by organic-inorganic composite membranes显示文摘Heavy use of conventional fertilizers can lead to negative environmental concerns.Controlled-release fertilizers(CRFs)can effectively reduce the amounts of fertilizers used,improve the availability of fer-tilizers,and which is conducive to the protection of the ecological environment and sustainable devel-opment of agriculture.Therefore,it is imperative to develop and use CRFs as an alternative to traditional fertilizers.This review aims to present the classification,raw material composition,benefits,release process,release mode,and manufacturing methods of fertilizers coated with organic-inorganic com-posite membranes(OICMs)in order to provide an overall update and summarize CRFs encapsulated by OICMs and provide an insight for future trends in the field of fertilizers.It is expected that utilizing CRFs encapsulated by OICMs and their characteristics for agricultural applications can provide innovative ideas and suggestions for developing novel CRFs suitable for modern and sustainable agriculture. | Xueping Wang Yongyan Yang Shuangling Zhong Qingye Meng Yiwei Li Jia Wang Yan Gao Xuejun Cui | 2024 | Particuology2024，84，1： | 0 |
| 9 | Preparation of Polyclonal Antisera of Dairy Cow S100A12 Protein显示文摘[Objective] This study aimed to prepare dairy cow anti-S100A12 antisera and develop a highly effective and sensitive immunological detection reagent for further investigation of the functions of dairy cow S100A12.[Method] Purified S100A12 protein was respectively emulsified with Freund's complete adjuvant and Freund's incomplete adjuvant as the antigen for immunizing New Zealand white rabbits to prepare the polyclonal antisera.The titer was detected using agar double diffusion assay and indirect enzyme-linked immunosorbent assay (ELISA) and the specificity was determined with Western Blot.[Result] The titer of anti-S100A12 antisera was 1∶8 as determined by agar double diffusion assay and over 1∶409 600 by ELISA.Western Blot result showed that the polyclonal antisera could be specifically combined with S100A12 protein.[Conclusion] The results indicated that anti-S100A12 polyclonal antibody with high titer and high specificity was successfully obtained, which provided a novel tool for further investigation of the functions of S100A12 gene. | Suizhong CAO Yafei CUI Xueping YAO Kang YONG Jishang LI Shumin YU | 2013 | Agricultural Biotechnology2013，2，3： | 0 |
| 10 | Construction and Identification of Mammary Gland-specific Expression Vector of Bovine Tracheal Antimicrobial Peptide (TAP)显示文摘[Objective] This study aimed to construct mammary gland-specific expression vector of bovine tracheal antimicrobial peptide (TAP) gene. [Method] TAP gene of dairy cattle was amplified from the mammary gland tissue by RT-PCR using a pair of primers which were designed according to bovine TAP cDNA sequence (NM_174776) in GenBank,and then cloned into pMD19-T Simple vector for sequencing. The recombinant plasmid was digested using EcoRⅠand KpnⅠ, the target gene fragment was recovered and inserted into general mammary gland-specific expression vector pBLG-EGFP harboring enhanced green fluorescent protein (EGFP) ,and transfected into bovine mammary epithelial cells ( bMEC) ,COS-7 cells and lactating rabbit mammary gland tissue by lipofectin transfection. The expression of green fluorescent protein in transfected cells was detected under fluorescence microscopy,and the expression of TAP mRNA in rabbit mammary gland tissue was detected by semi-quantity RT-PCR. [Result] The constructed mammary gland-specific expression vector pBLG-EGFP-TAP specifically expressed EGFP in transfected bMECs. In addition,semi-quantitative RT-PCR result showed that the expression level of TAP mRNA in rabbit mammary gland tissue was significantly enhanced after transfected with pBLG-EGFP-TAP. [Conclusion] The mammary gland-specific expression vector pBLG-EGFP-TAP was successfully constructed, which provided important materials for further investigation of expression characteristics of TAP gene and prevention of bovine mastitis by using genetic engineering technology. | Suizhong CAO Xueping YAO Yafei CUI Deying YANG Kang YONG Shumin YU Zongping LIU | 2013 | Agricultural Biotechnology2013，2，1： | 0 |
| 11 | Influence of aluminum solid fraction on property of steel-mushy aluminum bonding plate显示文摘The interfacial shear property of steel-mushy aluminum bonding plate was studied, and the relationship between aluminum solid fraction and the interfacial shear strength of bonding plate was determined. The results showed that when aluminum solid fraction is 34.3 %, the maximum interfacial shear strength of bonding plate is 71 .0 MPa. | Peng Zhang Yunhui Du Xueping Ren Yonglin Kang Hanwu Liu Jianzhong Cui Limin Ba(Material Science and Engineering School, University of Science and Technology Beijing, Beijing 100083, China)(Metal Forming Department, Northeastern University, Shenyang 110006 | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，3： | 0 |
| 12 | Plant virology in the 21st century in China:Recent advances and future directions显示文摘Plant viruses are a group of intracellular pathogens that persistently threaten global food security.Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years,including basic research and technologies for preventing and controlling plant viral diseases.Here,we review these milestones and advances,including the identification of new crop-infecting viruses,dissection of pathogenic mechanisms of multiple viruses,examination of multilayered interactions among viruses,their host plants,and virus-transmitting arthropod vectors,and in-depth interrogation of plantencoded resistance and susceptibility determinants.Notably,various plant virus-based vectors have also been successfully developed for gene function studies and target gene expression in plants.We also recommend future plant virology studies in China. | Jianguo Wu Yongliang Zhang Fangfang Li Xiaoming Zhang Jian Ye Taiyun Wei Zhenghe Li Xiaorong Tao Feng Cui Xianbing Wang Lili Zhang Fei Yan Shifang Li Yule Liu Dawei Li Xueping Zhou Yi Li | 2024 | Journal of Integrative Plant Biology2024，66，3： | 0 |
| 13 | Inverse-opal structured TiO_(2) regulating electrodeposition behavior to enable stable lithium metal electrodes显示文摘Lithium metal anode is almost the ultimate choice for high-energy density rechargeable batteries, but its uneven electrochemical dissolution-deposition characteristics lead to poor cycle stability and lithium dendrites safety problems. The fundamental solution to the problems is to interfere electrodeposition process of lithium metal so that it can be carried out reversibly and stably. In this work, an inverse-opal structured TiO2membrane with a thickness of only ~1 μm is designed to regulate the electrodeposition behavior of lithium metal, in which the ordered channels homogenize mass transfer process, the anatase TiO_(2)walls of the ion channels reduce desolvation barrier of solvated lithium-ions, and the spherical cavities with a diameter of ~300 nm confine migration of the adsorbed lithium atoms during electrocrystallization to diminish overpotential of lithium. These systematic effects cover and essentially change the whole process of electrodeposition of lithium metal and eliminate the possibility of lithium dendrite formation. The as-obtained lithium metal electrode delivers a Coulombic efficiency of 99.86% in the 100th cycle, and maintains a low deposition overpotential of 0.01 V for 800 h. | Xuewen Wu Shaolun Cui Minfei Fei Sheng Liu Xueping Gao Guoran Li | 2023 | Green Energy & Environment2023，8，6： | 0 |
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