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| 1 | Washed microbiota transplantation vs.manual fecal microbiota transplantation:clinical findings,animal studies and in vitro screening显示文摘Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual. | Ting Zhang Gaochen Lu Zhe Zhao Yafei Liu Quan Shen Pan Li Yaoyao Chen Haoran Yin Huiquan Wang Cicilia Marcella Bota Cui Lei Cheng Guozhong Ji Faming Zhang | 2020 | Protein & Cell2020，11，4： | 44 |
| 2 | Engineered targeting tLyp-1 exosomes as gene therapy vectors for efficient delivery of siRNA into lung cancer cells显示文摘Natural exosomes can express specific proteins and carbohydratemolecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer.However,the use of natural exosomes is restricted by their low transfection efficiency.Here,we report a novel targeting tLyp-1 exosome by gene recombinant engineering for delivery of siRNA to cancer and cancer stem cells.To reach such a purpose,the engineered tLyp-1-lamp2b plasmids were constructed and amplified in Escherichia coli.The tLyp-1-lamp2b plasmids were further used to transfect HEK293T tool cells and the targeting tLyp-1 exosomes were isolated from secretion of the transfected HEK293T cells.Afterwards,the artificially synthesized siRNA was encapsulated into targeting tLyp-1 exosomes by electroporation technology.Finally,the targeting siRNA tLyp-1 exosomes were used to transfect cancer or cancer stem cells.Results showed that the engineered targeting tLyp-1 exosomes had a nanosized structure(approximately 100 nm)and high transfection efficiency into lung cancer and cancer stem cells.The function verifications demonstrated that the targeting siRNA tLyp-1 exosomes were able to knock-down the target gene of cancer cells and to reduce the stemness of cancer stem cells.In conclusion,the targeting tLyp-1 exosomes are successfully engineered,and can be used for gene therapy with a high transfection efficiency.Therefore,the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy. | Jing Bai Jialun Duan Rui Liu Yafei Du Qian Luo Yinuo Cui Zhanbo Su Jiarui Xu Ying Xie Wanliang Lu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 5 |
| 3 | Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle显示文摘Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases. | Shuming Sun Han Liu Yan Hu Yanpeng Wang Mingri Zhao Yijun Yuan Yafei Han Yingying Jing Jin Cui Xiaoxiang Ren Xiao Chen Jiacan Su | 2023 | Bioactive Materials2023，，2： | 3 |
| 4 | Mouse knockout models reveal largely dispensable but context-dependent functions of IncRNAs during development显示文摘 | Xue Han Sai Luo Guangdun Peng Yuyang Lu Guizhong Cui Lichao Liu Pixi Yan Yafei Yin Wei Liu Ran Wang Jiejie Zhang Shanshan Ai Zai Chang Jie Na Aibin He Naihe Jing Xiaohua Shen | 2018 | Journal of Molecular Cell Biology2018，10，2： | 3 |
| 5 | Targeting lactate dehydrogenase A (LDHA) exerts antileukemic effects on T-cell acute lymphoblastic leukemia显示文摘Background:T-cell acute lymphoblastic leukemia(T-ALL)is an uncommon and aggressive subtype of acute lymphoblastic leukemia(ALL).In the serum of T-ALL patients,the activity of lactate dehydrogenase A(LDHA)is increased.We proposed that targeting LDHA may be a potential strategy to improve T-ALL outcomes.The current study was conducted to investigate the antileukemic effect of LDHA gene-targeting treatment on T-ALL and the underlying molecular mechanism.Methods:Primary T-ALL cell lines Jurkat and DU528 were treated with the LDH inhibitor oxamate.MTT,colony formation,apoptosis,and cell cycle assays were performed to investigate the effects of oxamate on T-ALL cells.Quantitative real-time PCR(qPCR)and Western blotting analyses were applied to determine the related signaling pathways.A mitochondrial reactive oxygen species(ROS)assay was performed to evaluate ROS production after T-ALL cells were treated with oxamate.A T-ALL transgenic zebrafish model with LDHA gene knockdown was established using CRISPR/Cas9 gene-editing technology,and then TUNEL,Western blotting,and T-ALL tumor progression analyses were conducted to investigate the effects of LDHA gene knockdown on T-ALL transgenic zebrafish.Results:Oxamate significantly inhibited proliferation and induced apoptosis of Jurkat and DU528 cells.It also arrested Jurkat and DU528 cells in G0/G1 phase and stimulated ROS production(all P<0.001).Blocking LDHA significantly decreased the gene and protein expression of c-Myc,as well as the levels of phosphorylated serine/threonine kinase(AKT)and glycogen synthase kinase 3 beta(GSK-3β)in the phosphatidylinositol 3′-kinase(PI3K)signaling pathway.LDHA gene knockdown delayed disease progression and down-regulated c-Myc mRNA and protein expression in T-ALL transgenic zebrafish.Conclusion:Targeting LDHA exerted an antileukemic effect on T-ALL,representing a potential strategy for T-ALL treatment. | Haizhi Yu Yafei Yin Yifang Yi Zhao Cheng Wenyong Kuang Ruijuan Li Haiying Zhong Yajuan Cui Lingli Yuan Fanjie Gong Zhihua Wang Heng Li Hongling Peng Guangsen Zhang | 2020 | Cancer Communications2020，40，10： | 2 |
| 6 | Transient thermo-mechanical analysis for bimorph soft robot based on thermally responsive liquid crystal elastomers显示文摘Thermally responsive liquid crystal elastomers (LCEs) hold great promise in applications of soft robots and actuators because of the induced size and shape change with temperature. Experiments have successfully demonstrated that the LCE based bimorphs can be effective soft robots once integrated with soft sensors and thermal actuators. Here, we present an analytical transient thermo-mechanical model for a bimorph structure based soft robot, which consists of a strip of LCE and a thermal inert polymer actuated by an ultra-thin stretchable open-mesh shaped heater to mimic the unique locomotion behaviors of an inchworm. The coupled mechanical and thermal analysis based on the thermo-mechanical theory is carried out to underpin the transient bending behavior, and a systematic understanding is therefore achieved. The key analytical results reveal that the thickness and the modulus ratio of the LCE and the inert polymer layer dominate the transient bending deformation. The analytical results will not only render fundamental understanding of the actuation of bimorph structures, but also facilitate the rational design of soft robotics. | Yun CUI Yafei YIN Chengjun WANG K. SIM Yuhang LI Cunjiang YU Jizhou SONG | 2019 | Applied Mathematics and Mechanics(English Edition)2019，40，7： | 1 |
| 7 | How do surface and edge effects alter the electronic properties of GaN nanoribbons 显示文摘 | Tang Qing Cui Yao Li Yafei | 2011 | Journal of Physical Chemistry C2011，115，5： | 1 |
| 8 | Preparation and capacitive properties of cobalt–nickel oxides/carbon nanotube composites显示文摘 | Zhen Fan Jinhua Chen Kunzai Cui Feng Sun Yan Xu Yafei Kuang | 2006 | Electrochimica Acta2006，，9： | 1 |
| 9 | Different Gene Networks Are Disturbed by Zika Virus Infection in A Mouse Microcephaly Model显示文摘The association of Zika virus(ZIKV)infection with microcephaly has raised alarm worldwide.Their causal link has been confirmed in different animal models infected by ZIKV.However,the molecular mechanisms underlying ZIKV pathogenesis are far from clear.Hence,we performed global gene expression analysis of ZIKV-infected mouse brains to unveil the biological and molecular networks underpinning microcephaly.We found significant dysregulation of the sub-networks associated with brain development,immune response,cell death,microglial cell activation,and autophagy amongst others.We provided detailed analysis of the related complicated gene networks and the links between them.Additionally,we analyzed the signaling pathways that were likely to be involved.This report provides systemic insights into not only the pathogenesis,but also a path to the development of prophylactic and therapeutic strategies against ZIKV infection. | Yafei Chang Yisheng Jiang Cui Li Qin Wang Feng Zhang Cheng-Feng Qin Qing-Feng Wu Jing Li Zhiheng Xu | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 0 |
| 10 | Multi-Point Communication System Based on CAN Bus显示文摘The paper introduced CAN(Controller Area Network) bus and CAN2.0 standard,the design of hardware frame-work multi-point communication based on CAN bus,and the software design method.At the same time,the system normal communication function and stability were shown in the experiment. | HE Yafei DONG Guiju DENG Likang CUI Tianshi | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，2： | 0 |
| 11 | Preparation of Polyclonal Antisera of Dairy Cow S100A12 Protein显示文摘[Objective] This study aimed to prepare dairy cow anti-S100A12 antisera and develop a highly effective and sensitive immunological detection reagent for further investigation of the functions of dairy cow S100A12.[Method] Purified S100A12 protein was respectively emulsified with Freund's complete adjuvant and Freund's incomplete adjuvant as the antigen for immunizing New Zealand white rabbits to prepare the polyclonal antisera.The titer was detected using agar double diffusion assay and indirect enzyme-linked immunosorbent assay (ELISA) and the specificity was determined with Western Blot.[Result] The titer of anti-S100A12 antisera was 1∶8 as determined by agar double diffusion assay and over 1∶409 600 by ELISA.Western Blot result showed that the polyclonal antisera could be specifically combined with S100A12 protein.[Conclusion] The results indicated that anti-S100A12 polyclonal antibody with high titer and high specificity was successfully obtained, which provided a novel tool for further investigation of the functions of S100A12 gene. | Suizhong CAO Yafei CUI Xueping YAO Kang YONG Jishang LI Shumin YU | 2013 | Agricultural Biotechnology2013，2，3： | 0 |
| 12 | Construction and Identification of Mammary Gland-specific Expression Vector of Bovine Tracheal Antimicrobial Peptide (TAP)显示文摘[Objective] This study aimed to construct mammary gland-specific expression vector of bovine tracheal antimicrobial peptide (TAP) gene. [Method] TAP gene of dairy cattle was amplified from the mammary gland tissue by RT-PCR using a pair of primers which were designed according to bovine TAP cDNA sequence (NM_174776) in GenBank,and then cloned into pMD19-T Simple vector for sequencing. The recombinant plasmid was digested using EcoRⅠand KpnⅠ, the target gene fragment was recovered and inserted into general mammary gland-specific expression vector pBLG-EGFP harboring enhanced green fluorescent protein (EGFP) ,and transfected into bovine mammary epithelial cells ( bMEC) ,COS-7 cells and lactating rabbit mammary gland tissue by lipofectin transfection. The expression of green fluorescent protein in transfected cells was detected under fluorescence microscopy,and the expression of TAP mRNA in rabbit mammary gland tissue was detected by semi-quantity RT-PCR. [Result] The constructed mammary gland-specific expression vector pBLG-EGFP-TAP specifically expressed EGFP in transfected bMECs. In addition,semi-quantitative RT-PCR result showed that the expression level of TAP mRNA in rabbit mammary gland tissue was significantly enhanced after transfected with pBLG-EGFP-TAP. [Conclusion] The mammary gland-specific expression vector pBLG-EGFP-TAP was successfully constructed, which provided important materials for further investigation of expression characteristics of TAP gene and prevention of bovine mastitis by using genetic engineering technology. | Suizhong CAO Xueping YAO Yafei CUI Deying YANG Kang YONG Shumin YU Zongping LIU | 2013 | Agricultural Biotechnology2013，2，1： | 0 |
| 13 | Hospitalization for Congenital Heart Disease in Beijing:Patient Characteristics and Temporal Trends显示文摘Background:The hospitalization burden of congenital heart disease(CHD)at the national or regional level in China remains unknown.We aimed to evaluate the recent patient characteristics and temporal trends of hospitalizations for CHD in Beijing.Methods:Patients hospitalized for CHD in Beijing from 2007 to 2011 were identified from the Hospital Discharge Information System.Patient characteristics were recorded,and trends in hospitalization rates were analyzed by Poisson regression after adjustment for age and sex.Results:A total of 53,064 patients were admitted for CHD in Beijing during the study period,among whom 50.5% were children younger than 5 years,30.0% were adults,18.5% had severe CHD,86.9% were nonpermanent Beijing residents,and 81.3% were admitted to cardiac-specific tertiary hospitals.The hospitalization rate increased from 10.2 per 100,000 population in 2007 to 12.4 per 100,000 population in 2011,representing a signifi cant increase after adjustment for age and sex(P=0.009).However,the trends varied in different groups.The rates halved in 2011 for both nonsevere CHD and severe CHD compared with the rates in 2010,whereas increasing trends were noticed in children aged 1–4 years and adults for nonsevere CHD across the study period.Conclusions:The overall rate of CHD hospitalization has been increasing in Beijing,whereas not all patient groups have the same trend.The hospitalization rate decreased among infants,while the rate for nonsevere CHD increased signifi cantly among adults.These findings will be of importance for future allocation of health resources. | Yafei Cui Dong Zhao Jiayi Sun Miao Wang Yinglong Liu Jing Liu | 2017 | Cardiovascular Innovations and Applications2017，，B02： | 0 |
| 14 | Visual analysis of flow and diffusion of hemolytic agents and hematomas显示文摘The elimination of intracranial hematomas has received widespread attention and the interactions between hemolytic agents and hematomas have become a hot research topic.In this study,we used the Navier-Stokes equation to describe the flow control equation for hemolytic agents in a tube and used Fick’s law and the Maxwell-Stefan diffusion theory to describe the diffusion and mass transfer of hemolytic agents and hematomas.The physical fields and initial boundary conditions were set according to the parametric properties of the fluid and drainage tube.The COMSOL Multiphysics software was used to simulate the streamline distribution of hemolytic agents in a bifurcated drainage tube.Additionally,the diffusion behaviors of the hemolytic agents into hematomas were simulated and visual analysis of coupled multiphysics was performed to realize the digitization and visualization of engineering fluid problems and contribute to the field of medical engineering. | Yafei Zhu Mengyao Bao Miaomiao Jiang Jincai Chang Jianzhong Cui | 2021 | Visual Computing for Industry,Biomedicine,and Art2021，4，1： | 0 |
| 15 | Coalbed methane desorption characteristics controlled by coalification and its implication on gas co-production from multiple coal seams显示文摘In this work,CH4 isothermal adsorption measurements were carried out on 64 coal samples collected from western Guizhou Province of China,and the coalbed methane(CBM)desorption processes were quantitatively analyzed.The results show that the Langmuir volume and the Langmuir pressure are controlled by coalification,and tend to increase as the vitrinite reflectance changes from 0.98% to 4.3%.Based on a division method of CBM desorption stages,the CBM desorption process were divided into four stages(inefficient,slow,fast and sensitive desorption stages)by three key pressure nodes(the initial,turning and sensitive pressures).The fast and sensitive desorption stages with high desorption efficiency are the key for achieving high gas production.A theoretical chart of the critical desorption pressure(P_(cd))and its relationship with different pressure nodes was established.The higher-rank coals have the higher initial,turning and sensitive pressures,with larger difference between pressure nodes.Most CBM wells only undergo partial desorption stages due to the differences in P_(cd) caused by the present-gas content.Under the same gas content conditions,the higher the coal rank,the less desorption stages that CBM needs to go through.During coalbed methane co-production from multiple coal seams within vertically superposed pressure systems,the reservoir pressure,the P_(cd),the initial working liquid level(WLL)height,and coal depth are key factors for evaluating whether coal seams can produce CBM simultaneously.It must be ensured that each production layer enters at least the fast desorption stage prior to that the WLL was lower than the depth of each layer.Only on this basis can all layers achieve the maximum gas production. | Bin ZHANG Yafei ZHANG Suping ZHAO Wei HE Shu TAO Zhejun PAN Yi CUI | 2023 | Frontiers of Earth Science2023，17，1： | 0 |
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