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| 1 | Regulation of male fertility by the opioid system 显示文摘 | Subirrn N Casis L Irazusta J | 2011 | Md Med2011，17，78： | 1 |
| 2 | Ontogeny of prolyl endopeptidase and pyroglutamyl peptidase I in rat tissues显示文摘 | Agirregoitia N Casis L Gil J | 2007 | Regul Pept2007，3，139： | 1 |
| 3 | Periocular photodynamic therapy for squamous intra-epidermal carcinoma显示文摘 | Casie Chetty N Hemmant B Skellett AM | 2014 | J Dermatolog Treat2014，25，6： | 1 |
| 4 | 显示文摘 | Casy G Johnson N B | 1998 | J Org Chem1998，63，18： | 1 |
| 5 | Biodegration study of Psedo- monas, of flexible polyurethane foams derived from castor oil 显示文摘 | Sponton M Casis N Mazo P | 2013 | International Biodeterioration & Biodegradation2013，85，： | 1 |
| 6 | Cross-sectional evaluation of circulating hepatitis B virus RNA and DNA: Different quasispecies?显示文摘BACKGROUND Different forms of pregenomic and other hepatitis B virus(HBV)RNA have been detected in patients’sera.These circulating HBV-RNAs may be useful for monitoring covalently closed circular DNA activity,and predicting hepatitis B eantigen seroconversion or viral rebound after nucleos(t)ide analog cessation.Data on serum HBV-RNA quasispecies,however,is scarce.It is therefore important to develop methodologies to thoroughly analyze this quasispecies,ensuring the elimination of any residual HBV-DNA.Studying circulating HBV-RNA quasispecies may facilitate achieving functional cure of HBV infection.AIM To establish a next-generation sequencing(NGS)methodology for analyzing serum HBV-RNA and comparing it with DNA quasispecies.METHODS Thirteen untreated chronic hepatitis B patients,showing different HBV-genotypes and degrees of severity of liver disease were enrolled in the study and a serum sample with HBV-DNA>5 Log10 IU/mL and HBV-RNA>4 Log10 copies/mL was taken from each patient.HBV-RNA was treated with DNAse I to remove any residual DNA,and the region between nucleotides(nt)1255-1611 was amplified using a 3-nested polymerase chain reaction protocol,and analyzed with NGS.Variability/conservation and complexity was compared between HBV-DNA and RNA quasispecies.RESULTS No HBV-DNA contamination was detected in cDNA samples from HBV-RNA quasispecies.HBV quasispecies complexity showed heterogeneous behavior among patients.The Rare Haplotype Load at 1%was greater in DNA than in RNA quasispecies,with no statistically significant differences(P=0.1641).Regarding conservation,information content was equal in RNA and DNA quasispecies in most nt positions[218/357(61.06%)].In 102 of the remaining 139(73.38%),HBV-RNA showed slightly higher variability.Sliding window analysis identified 4 hyper-conserved sequence fragments in each quasispecies,3 of them coincided between the 2 quasispecies:nts 1258-1286,1545-1573 and 1575-1604.The 2 hyper-variable sequence fragments also coincided:nts 1311-1344 and 1461-1485.Sequences between nts 1519-1543 and 1559-1587 were only hyper-conserved in HBV-DNA and RNA,respectively.CONCLUSION Our methodology allowed analyzing HBV-RNA quasispecies complexity and conservation without interference from HBV-DNA.Thanks to this,we have been able to compare both quasispecies in the present study. | Selene Garcia-Garcia Maria Francesca Cortese David Tabernero Josep Gregori Marta Vila Beatriz Pacín Josep Quer Rosario Casillas Laura Castillo-Ribelles Roser Ferrer-Costa Ariadna Rando-Segura Jesús Trejo-Zahínos Tomas Pumarola Ernesto Casis Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2021 | World Journal of Gastroenterology2021，27，41： | 1 |
| 7 | Health Care Costs of Complex Perianal Fistula in Crohn’s Disease显示文摘 | M. Chaparro C. Zanotti P. Burgue?o I. Vera F. Bermejo I. Marín-Jiménez C. Yela P. López M. D. Martín C. Taxonera B. Botella R. Pajares A. Ponferrada M. Calvo A. Algaba L. Pérez B. Casis J. Maté J. Orofino N. Lara M. García-Losa X. Badia J. P. Gisbert | 2013 | Digestive Diseases and Sciences2013，，12： | 1 |
| 8 | Delphi consensus statement: Quality indicators for Inflammatory Bowel Disease Comprehensive Care Units显示文摘 | Xavier Calvet Julián Panés Noelia Alfaro Joaquin Hinojosa Beatriz Sicilia Marta Gallego Ildefonso Pérez Pablo Lázaro y de Mercado Fernando Gomollón Physicians: Xavier Aldeguer Rafael Alós Montserrat Andreu Manu Barreiro Fernando Bermejo Bego?a Casis Eugen | 2013 | Journal of Crohn’s and Colitis2013，，： | 1 |
| 9 | Local renin angiotensin system and sperm DNA fragmentation显示文摘The renin angiotensin system(RAS)appears to influence male fertility at multiple levels.In this work,we analyzed the relationship between the RAS and DNA integrity.Fifty male volunteers were divided into two groups(25 each):control(DNA fragmentation≤20%)and pathological(DNA fragmentation>20%)cases.Activities of five peptidases controlling RAS were measured fluorometrically:prolyl endopeptidase(which converts angiotensin[A]I and A II to A 1–7),neutral endopeptidase(NEP/CD10:A I to A 1–7),aminopeptidase N(APN/CD13:A III to A IV),aminopeptidase A(A II to A III)and aminopeptidase B(A III to A IV).Angiotensin-converting enzyme(A I to A II),APN/CD13 and NEP/CD10 were also assessed by semiquantitative cytometry and quantitative flow cytometry assays,as were the receptors of all RAS components:A II receptor type 1(AT1R),A II receptor type 2(AT2R),A IV receptor(AT4R or insulin-regulated aminopeptidase[IRAP]),(pro)renin receptor(PRR)and A 1–7 receptor or Mas receptor(MasR)None of the enzymes that regulate levels of RAS components,except for APN/CD13(decrease in fragmented cells),showed significant differences between both groups.Micrographs of RAS receptors revealed no significant differences in immunolabeling patterns between normozoospermic and fragmented cells.Labeling of AT1R(94.3%normozoospermic vs 84.1%fragmented),AT4R(96.2%vs 95.3%)and MasR(97.4%vs 87.2%)was similar between the groups.AT2R(87.4%normozoospermic vs 63.1%fragmented)and PRR(96.4%vs 48.2%)were higher in non-fragmented spermatozoa.These findings suggest that fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides. | María Victoria Aparicio Prieto María Victoria Rodríguez Gallego Asier Valdivia Palacín Yosu Franco Iriarte Gotzone Hervás Barbara Enrique Echevarría Orella Luis Casis Saenz | 2022 | Asian Journal of Andrology2022，24，2： | 0 |
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