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| 1 | The effects of straw‑returning and inorganic K fertilizer on the carbon–nitrogen balance and reproductive growth of cotton显示文摘Background:Many studies have indicated that straw-returning could meet part or even all of the potassium(K)demand for crop growth in the field,but few have compared the effects of crop straw as K source and inorganic K fertilizer on carbon–nitrogen(C–N)balance of cotton and the reproductive growth.To address this,field experiments were conducted using the cotton cultivar,Siza 3,under there treatments(CK as control group one,no crop straw and inorganic K fertilizer were applied;K150 as control group two,150 kg·ha^(-1) of K2O was applied;and W9000,9000 kg·ha^(-1) wheat straw,which could provide K2O about 150 kg·ha^(-1),was incorporated into soil).Results:Although the final reproductive organ biomass did not differ between W9000 and K150,W9000 had a higher ratio of reproductive organ biomass to total biomass(RRT),suggesting that straw-returning was more conducive to the allocation of biomass to reproductive organs.The theoretical maximum biomass of reproductive organ was higher,but the average and maximum accumulation rates of reproductive organ biomass were 2.8%∼8.3%and 2.5%∼8.2%lower under W9000 than K150.Also,the duration of rapid-accumulation period for reproductive organ biomass(T)was 2.0∼2.8 d longer under W9000 than K150,which was a reason for the higher RRT under W9000.Straw-returning altered the dynamics of leaf K with the growth period,so that W9000 had a more drastic effect on leaf C metabolism than K150.Consequently,lower soluble sugar/free amino acid and C/N ratios were measured under W9000 than K150 at boll-setting(BSS)and boll-opening(BOS)stages.Higher leaf net photosynthetic rate,sucrose phosphate synthase and sucrose synthase activities,and lower acid invertase activity were observed under W9000 than K150 at BSS and BOS and these were more conducive to sucrose accumulation.However,less sucrose was measured under W9000 than K150 at these stages.This should be because straw-returning promoted the assimilate transport capacity when compared with inorganic K fertilizer application,which also explained the higher RRT under W9000 than K150.The lower acid invertase activity under W9000 inhibited the conversion of sucrose to other sugars,hence lower contents of soluble sugar and starch were measured under W9000 than K150.Conclusion:Under low K condition,crop straw as K source can increase the assimilate transport from source to sink,leading to lower C/N ratio in leaf and higher allocation of biomass to reproductive organs than inorganic K fertilizer. | HU Wei YU Chaoran ZHAO Wenqing LIU Ruixian YANG Changqinand ZHOU Zhiguo | 2021 | Journal of Cotton Research2021，4，4： | 2 |
| 2 | Correction to:Efficient derivation of extended pluripotent stem cells from NOD-scid II2rg^-/-mice显示文摘Figure 1.Generation of NOD-scid II2rg^-1-extended pluripotent stem cells.(A)Schematic of two approaches used for generating NOD-scid II2rg^-1-extended pluripotent stem cells:de novo derivation from blastocysts(upper panels)and chemical reprogramming from embryonic fibroblasts(lower panels).(B)Phase-contrast images of de novo derived outgrowth and EPS colonies for 17 passages in LCDM medium.Scale bars,100 pm.(C)qRT-PCR analysis of XEN marker genes expression during the chemical induction process(day 16).Error bars indicate SEM(n=2).(D)Co-immunostaining of XEN marker genes during the chemical induction process(day 16).Upper panels:GATA6 and SALL4;lower panels:SOX17 and SALL4.Scale bars,100 pm. | Yaqin Du Ting Wang Jun Xu Chaoran Zhao Haibo Li Yao Fu Yaxing Xu Liangfu Xie Jingru Zhao Weifeng Yang Ming Yin Jinhua Wen Hongkui Deng | 2019 | Protein & Cell2019，10，2： | 1 |
| 3 | Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation显示文摘One major strategy to generate genetically modified mouse models is gene targeting in mouse embryonic stem(ES)cells,which is used to produce gene-targeted mice for wide applications in biomedicine.However,a major bottleneck in this approach is that the robustness of germiine transmission of gene-targeted ES cells can be significantly reduced by their genetic and epigenetic instability after long-term culturing,which impairs the efficiency and robustness of mouse model generation.Recently,we have established a new type of pluripotent cells termed extended pluripotent stem(EPS)cells,which have superior developmental potency and robust germline competence compared to conventional mouse ES cells.In this study,we demonstrate that mouse EPS cells well maintain developmental potency and genetic stability after long-term passage.Based on gene targeting in mouse EPS cells,we established a new approach to directly and rapidly generate gene-targeted mouse models through tetraploid complementation,Haibo Li and Chaoran Zhao contributed equally to this work.Electronic supplementary material The online version of this article(http://gffzzd3cc09b8251d45dfsbwx0wcnnfok966n0.ffgz.tsg.suse.edu.cn/10.1007/s13238-018-0556-1)contains supplementary material,which is available to authorized users.which could be accomplished in approximately 2 months.Importantly,using this approach,we successfully constructed mouse models in which the human interleukin 3(IL3)or interleukin 6(IL6)gene was knocked into its corresponding locus in the mouse genome.Our study demonstrates the feasibility of using mouse EPS cells to rapidly generate mouse models by gene targeting,which have great application potential in biomedical research. | Haibo Li Chaoran Zhao Jun Xu Yaxing Xu Chunmei Cheng Yinan Liu Ting Wang Yaqin Du Liangfu Xie Jingru Zhao Yanchuang Han Xiaobao Wang Yun Bai Hongkui Deng | 2019 | Protein & Cell2019，10，1： | 1 |
| 4 | Traditional Chinese medicines for non-small cell lung cancer: Therapies and mechanisms显示文摘The most common subtype of lung cancer is non-small cell lung cancer(NSCLC), which has a poor prognosis and seriously threatens the health of human beings. The multidisciplinary comprehensive treatment model has gradually become the mainstream of NSCLC treatment. Traditional Chinese medicine(TCM) can be used effectively either as an adjunctive therapy or alone throughout the NSCLC therapy,which has a significant impact on survival, quality of life, and reduction of toxicity. Therefore, this paper reviewed the theoretical basis, the latest clinical application, and combined treatment mechanisms in order to explore the advantage stage of TCM treatment and the synergistic therapeutic mechanisms. | Fanming Kong Chaoran Wang Linlin Zhao Dongying Liao Xiaoqun Wang Binxu Sun Peiying Yang Yingjie Jia | 2023 | Chinese Herbal Medicines2023，15，4： | 0 |
| 5 | Evaluating and improving the interpretability of item embeddings using item-tag relevance information显示文摘Matrix factorization(MF)methods have superior recommendation performance and are flexible to incorporate other side information,but it is hard for humans to interpret the derived latent factors.Recently,the item-item cooccurrence information is exploited to learn item embeddings and enhance the recommendation performance.However,the item-item co-occurrence information,constructed from the sparse and long-tail distributed user-item interaction matrix,is over-estimated for rare items,which could lead to bias in learned item embeddings.In this paper,we seek to evaluate and improve the interpretability of item embeddings by leveraging a dense item-tag relevance matrix.Specifically,we design two metrics to quantitatively evaluate the interpretability of item embeddings from different viewpoints:interpretability of individual dimensions of item embeddings and semantic coherence of local neighborhoods in the latent space.We also propose a tag-informed item embedding(TIE)model that jointly factorizes the user-item interaction matrix,the item-item co-occurrence matrix and the item-tag relevance matrix with shared item embeddings so that different forms of information can co-operate with each other to learn better item embeddings.Experiments on the MovieLens20M dataset demonstrate that compared with other state-of-the-art MF methods,TIE achieves better top-N recommendations,and the relative improvement is larger when the user-item interaction matrix becomes sparser.By leveraging the itemtag relevance information,individual dimensions of item embeddings are more interpretable and local neighborhoods in the latent space are more semantically coherent;the bias in learned item embeddings are also mitigated to some extent. | Tao LIAN Lin DU Mingfu ZHAO Chaoran CUI Zhumin CHEN Jun MA | 2020 | Frontiers of Computer Science2020，14，3： | 0 |
| 6 | Graph CA: Learning From Graph Counterfactual Augmentation for Knowledge Tracing显示文摘With the popularity of online learning in educational settings, knowledge tracing(KT) plays an increasingly significant role. The task of KT is to help students learn more effectively by predicting their next mastery of knowledge based on their historical exercise sequences. Nowadays, many related works have emerged in this field, such as Bayesian knowledge tracing and deep knowledge tracing methods. Despite the progress that has been made in KT, existing techniques still have the following limitations: 1) Previous studies address KT by only exploring the observational sparsity data distribution, and the counterfactual data distribution has been largely ignored. 2) Current works designed for KT only consider either the entity relationships between questions and concepts, or the relations between two concepts, and none of them investigates the relations among students, questions, and concepts, simultaneously, leading to inaccurate student modeling. To address the above limitations,we propose a graph counterfactual augmentation method for knowledge tracing. Concretely, to consider the multiple relationships among different entities, we first uniform students, questions, and concepts in graphs, and then leverage a heterogeneous graph convolutional network to conduct representation learning.To model the counterfactual world, we conduct counterfactual transformations on students’ learning graphs by changing the corresponding treatments and then exploit the counterfactual outcomes in a contrastive learning framework. We conduct extensive experiments on three real-world datasets, and the experimental results demonstrate the superiority of our proposed Graph CA method compared with several state-of-the-art baselines. | Xinhua Wang Shasha Zhao Lei Guo Lei Zhu Chaoran Cui Liancheng Xu | 2023 | IEEE/CAA Journal of Automatica Sinica2023，10，11： | 0 |
| 7 | Efficient derivation of extended pluripotent stem cells from NOD-scid II2rg-/-mice显示文摘Recently we have established a new culture condition enabling the derivation of extended pluripotent stem(EPS)cells,which,compared to conventional pluripotent stem cells,possess superior developmental potential and germline competence.However,it remains unclear whether this condition permits derivation of EPS cells from mouse strains that are refractory or non-permissive to pluripotent cell establishment.Here,we show that EPS cells can be robustly generated from non-permissive NOD-sc/d Il2rg 1 mice through de novo derivation from blastocysts.Furthermore,these cells can also be efficiently generated by chemical reprogramming from embryonic NOD-sc/d II2rg-/-fibroblasts.NOD-sc/d II2rg-/-EPS cells can be expanded for more than 20 passages with genomic stability and can be genetically modified through gene targeting.Notably,these cells contribute to both embryonic and extraembryonic lineages in vivo.More importantly,they can produce chimeras and integrate into the E13.5 genital ridge.Our study demonstrates the feasibility of generating EPS cells from refractory mouse strains,which could potentially be a general strategy for deriving mouse pluripotent cells.The generation of NOD-sc/d II2rg-/-Yaqin Du and Ting Wang contributed equally to this work.Electronic supplementary material The online version of this article(http://gffzzd3cc09b8251d45dfsbwx0wcnnfok966n0.ffgz.tsg.suse.edu.cn/10.1007/s13238-018-0558-z)contains supplementary material,which is available to authorized users.EPS cell lines permits sophisticated genetic modification in NOD-scid II2rg-/-mice,which may greatly advance the optimization of humanized mouse models for biomedical applications. | Yaqin Du Ting Wang Jun Xu Chaoran Zhao Haibo Li Yao Fu Yaxing Xu Liangfu Xie Jingru Zhao Weifeng Yang Ming Yin Jinhua Wen Hongkui Deng | 2019 | Protein & Cell2019，10，1： | 0 |
| 8 | 建立具有体内胚内和胚外发育潜能的多能干细胞显示文摘文章简介在已知的体外培养的干细胞类型当中,多能干细胞具有最高的发育潜能,能够形成构成成年个体的所有细胞类型。但是,多能干细胞很难在体内发育过程中形成胚外的胎盘组织。 | Yang Yang Bei Liu Jun Xu Jinlin Wang Jun Wu Cheng Shi Yaxing Xu Jiebin Dong Chengyan Wang Weifeng Lai Jialiang Zhu Liang Xiong Dicong Zhu Xiang Li Weifeng Yang Takayoshi Yamauchi Atsushi Sugawara Zhongwei Li Fangyuan Sun Xiangyun Li Chen Li Aibin He Yaqin Du Ting Wang Chaoran Zhao Haibo Li Xiaochun Chi Hongquan Zhang Yifang Liu Cheng Li Shuguang Duo Ming Yin 沈浣 Juan Carlos Izpisua Belmonte 邓宏魁 | 2018 | 科学新闻2018，0，4： | 0 |
| 9 | Time-lapse changes of in vivo injured neuronal substructures in the central nervous system after low energy two-photon nanosurgery显示文摘There is currently very little research regarding the dynamics of the subcellular degenerative events that occur in the central nervous system in response to injury. To date, multi-photon excitation has been primarily used for imaging applications; however, it has been recently used to selectively disrupt neural structures in living animals. However, understanding the complicated processes and the essential underlying molecular pathways involved in these dynamic events is necessary for studying the underlying process that promotes neuronal regeneration. In this study, we introduced a novel method allowing in vivo use of low energy(less than 30 m W) two-photon nanosurgery to selectively disrupt individual dendrites, axons, and dendritic spines in the murine brain and spinal cord to accurately monitor the time-lapse changes in the injured neuronal structures. Individual axons, dendrites, and dendritic spines in the brain and spinal cord were successfully ablated and in vivo imaging revealed the time-lapse alterations in these structures in response to the two-photon nanosurgery induced lesion. The energy(less than 30 m W) used in this study was very low and caused no observable additional damage in the neuronal sub-structures that occur frequently, especially in dendritic spines, with current commonly used methods using high energy levels. In addition, our approach includes the option of monitoring the time-varying dynamics to control the degree of lesion. The method presented here may be used to provide new insight into the growth of axons and dendrites in response to acute injury. | Zhikai Zhao Shuangxi Chen Yunhao Luo Jing Li Smaranda Badea Chaoran Ren Wutian Wu | 2017 | Neural Regeneration Research2017，12，5： | 0 |
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