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| 1 | Rapid mitogen-activated protein kinase by basic fibroblast growth factor in rat intestine after ischemia/reperfusion injury显示文摘AIM: Previous studies showed that exogenous basic fibroblast growth factor (bFGF or FGF-2) could improve physiological dysfunction after intestinal ischemia/reperfusion (I/R) injury. However, the mechanisms of this protective effect of bFGF are still unclear. The present study was to detect the effect of bFGF on the activities of mitogen-activated protein kinase (MAPK) signaling pathway in rat intestine after I/R injury, and to investigate the protective mechanisms of bFGF on intestinal ischemiain jury.METHODS: Rat intestinal I/R injury was produced by clamping the superior mesenteric artery (SMA) for 45 minutes and followed by reperfusion for 48 hours. Seventy-eight Wistar rats were used and divided randomly into sham-operated group (A), normal saline control group (B),bFGF antibody pre-treated group (C), and bFGF treated group (D). In group A, SMA was separated without occlusion. In groups B, C and D, SMA was separated and occluded for 45 minutes, then, released for reperfusion for 48 hours. After the animals were sacrificed, blood and tissue samples were taken from the intestine 45 minutes after ischemia in group A and 2, 6, 24, and 48 hours after reperfusion in the other groups. Phosphorylated forms of p42/p44 MAPK, p38 MAPK and stress activated protein kinase/C-Jun N-terminal kinase (SAPK/JNK) were measured by immunohistochemistry. Plasma levels of D-lactate were examined and histological changes were observed under the light microscope.RESULTS: Intestinal I/R injury induced the expression of p42/p44 MAPK, p38 MAPK, and SAPK/JNK pathways and exogenous bFGF stimulated the early activation of p42/p44 MAPK and p38 MAPK pathways. The expression of phosphorylated forms of p42/p44 MAPK was primarily localized in the nuclei of crypt cells and in the cytoplasm and nuclei of villus cells. The positive expression of p38 MAPK was localized mainly in the nuclei of crypt cells, very few in villus cells. The activities of p42/p44 MAPK and p38 MAPK peaked 6 hours after reperfusion in groups B and C,while SAPK/JNK peaked 24 hours after reperfusion. The activities of p42/p44 MAPK and p38 MAPK peaked 2 hours after reperfusion in group D and those of SAPK/JNK were not changed in group B. D-lactate levels and HE staining showed that the intestinal barrier was damaged severely 6 hours after reperfusion, however, histological structures were much improved 48 hours after reperfusion in group D than in the other groups.CONCLUSION: The results indicate that intestinal I/R injury stimulates the activities of MAPK pathways, and that p42/p44 MAPK and p38MAPK activities are necessary for the protective effect of exogenous bFGF on intestinal I/R injury.The protective effect of bFGF on intestinal dysfunction may be mediated by the early activation of p42/p44 MAPK and p38 MAPK signaling pathways. | Xiao-Bing Fu Yin-Hui Yang Tong-Zhu Sun Wei Chen Jun-You Li Zhi-Yong Sheng Wound Healing and Cell Biology Laboratory,Burns Institute,304 Hospital,Trauma Center of Postgraduate Medical College,Beijing 100037,China | 2003 | World Journal of Gastroenterology2003，9，6： | 11 |
| 2 | 儿童期肥胖、成年期肥胖和心血管风险因子显示文摘过去30年,儿童超重或肥胖的发生率明显增加[1]。儿童期肥胖是死亡率增加的预测因子,这主要是由于儿童期肥胖可导致心血管疾病发生率增加。相关的预测研究提示"肥胖流行症"可能改变目前心血管疾病死亡率下降的趋势,使现今儿童的寿命缩短。 | Juonala M Magnussen CG Berenson GS Venn A Burns TL Sabin MA Srinivasan SR Daniels SR Davis PH Chen W Sun C Cheung M Viikari JS Dwyer T Raitakari OT 叶鹏 | 2012 | 中华高血压杂志2012，20，1： | 6 |
| 3 | Effect of the current density on electrodepositing alpha-lead dioxide coating on aluminum substrate显示文摘The α-PbO2 electrodes are prepared by anodic electrodeposition on A1/conductive coating electrode from alkaline plumbite solutions in order to investigate the effect of the different current densities on the properties of α-PbO2 electrodes. The physic-ochemical properties of the α-PbO2 electrodes are analyzed by using SEM, EDS, XRD, Tafel plot, linear sweep voltammetry (LSV) and A.C. impedance. A compact and uniform layer of lead dioxide was obtained at the current density of 3 mA·cm-2. A further increase in current density results in smaller particles with high porosity. EDS and XRD analyses have shown that the PbO2 deposited in alkaline conditions is highly non stoichiometric, and the PbO impurities are formed on the surface layer besides the α-PbO2. The corrosion resistance of α-PbO2 at the low current density is superior to that of the high current density. It can be attributed to a porous layer of deposited films at high current densities. When used as anodes for oxygen evolution in aqueous Zn2+ 50 g·L-1, H2SO4 150 g·L-1, the Al/conductive coating/α-PbO2 exhibits lower potential compared to Pb electrode. Al/conductive coating/α-PbO2 electrode with the best electrocatalytic activity was obtained at current density of 1 mA·cm-2. The lowest roughness factor was obtained at 1 mA·cm-2. | Burning CHEN Zhongcheng GUO Hui HUANG Xianwan YANG Yuandong CAO | 2009 | Acta Metallurgica Sinica(English Letters)2009，22，5： | 6 |
| 4 | Dual functions of Streptococcus salivarius urease显示文摘 | Chen YM Weaver CA Burne RA | 2000 | J Bacteriol2000，182，： | 1 |
| 5 | Severe necrotizing fasciitis in a human immunodeficiency virus-positive patient caused by methicillin-resistant Staphylococcus aureus显示文摘 | Olsen RJ Burns KM Chen L | 2008 | J Clin Microbiol2008，46，3： | 1 |
| 6 | cis-Acting elements that regulate the low-pH-inducible urease operon of Streptococcus salivarius显示文摘 | Chen YM Betzenhauser MJ Burne RA | 2002 | Microbiol2002，148，： | 1 |
| 7 | Dual Functions of Streptococcus salivarius Urease 显示文摘 | CHEN Y Y WEAVER C A BURNE R A | 2000 | Journal of Bacteriology2000，182，： | 1 |
| 8 | Streptococcus salivarius urease: genetic and biochemical characterization and expression in a dental plaque streptococcus 显示文摘 | Chen YY Clancy KA Burne RA | 1996 | Infect Immun1996，64，2： | 1 |
| 9 | Parameter estimation and capacity fade analysis of lithium batteries using re- formulated models 显示文摘 | RAMADESIGAN V CHEN K J BURNS N A | 2011 | Journal of the Electrochemical Society2011，158，9： | 1 |
| 10 | Fiber-optic gyroscopes with broad-band source显示文摘 | BURNS W K CHEN C L MOELLER R P | 1983 | Journal of Lightwave Technology1983，1，： | 1 |
| 11 | Se - 79: geochemical and crystallo-chemical retardation mechanisms显示文摘 | Chen FR Burns PC Ewing RC | 1999 | Journal of Nuclear Materials1999，275，1： | 1 |
| 12 | Malignant Astrocytomas Originate from Neural Stem/Progenitor Cells in a Somatic Tumor Suppressor Mouse Model显示文摘 | Sheila Alcantara Llaguno Jian Chen Chang-Hyuk Kwon Erica L. Jackson Yanjiao Li Dennis K. Burns Arturo Alvarez-Buylla Luis F. Parada | 2009 | Cancer Cell2009，，1： | 1 |
| 13 | Severe necrotizing fasciitis in a hu-man immunodefi ciency virus -positive patient caused by methicillin -resistant Staphylococcus aurcus 显示文摘 | Olsen R J Burns KM Chen L | 2008 | J Clin Microbiol2008，46，3： | 1 |
| 14 | Multiple independent emer- gences of type 2 vaccine-derived polioviruses during a large outbreak in northern Nigeria显示文摘 | Burns C C Shaw J Jorba J Bukbuk D Adu F Gumede N Pate M A Abanida E A Gasasira A Iber J Chen Q Vincent A Chenoweth P Henderson E Wannemuehler K Naeem A Umarni R N Nishimura Y Shimizu H Baba M Adeniji A Williams A J Kil- patrick D R Oberste M S Wassilak S G Tomori O Pallansch M A Kew O | 2013 | J Virol2013，87，9： | 1 |
| 15 | Cyclophilin A Promotes Cardiac Hypertrophy in Apolipoprotein E–Deficient Mice显示文摘 | Kimio Satoh Patrizia Nigro Asad Zeidan Nwe Nwe Soe Fabrice Jaffré Masayoshi Oikawa Michael R. O?Dell Zhaoqiang Cui Prashanthi Menon Yan Lu Amy Mohan Chen Yan Burns C. Blaxall Bradford C. Berk | 2011 | Arteriosclerosis Thrombosis and Vascular Biology2011，，5： | 1 |
| 16 | Fiber-optic gyroscopes with broad-band sources 显示文摘 | Burns W K Chen C Moeller R P | 1983 | Journal of Lightwave Technology1983，1，1： | 1 |
| 17 | Parameter estimation and capacity fade analysis of lithium-ion batteries using first-principles-based efficient reformulated models 显示文摘 | Ramadesigan V Chen K J Burns N A | 2011 | Journal of the Electro- chemical Society2011，158，9： | 1 |
| 18 | Characteristics and mechanisms of the annual asymmetry of thermospheric mass density显示文摘在这份报纸,全球性平均, thermospheric 总数团密度,在从 1967 ~ 2006 被追踪的 250, 400,和 550 km 源于 5000 个目标的轨道,习惯于份量上学习 thermospheric 质量的年度不对称现象密度和它的机制。结果证明那 thermospheric 团密度有重要年度不对称现象,它从年改变了到年。在三高度的年度不对称现象同时地变化了,它的绝对值与高度增加了。结果建议在被差别在一年以内在在二至和太阳的活动的随机的变化之间的太阳地球远处引起的太阳的 EUV 放射有年度不对称现象。在在在 thermospheric 温度并且这样的一个年度变化的放射结果的这个变化中立气体的规模高度,并且是 thermospheric 质量的年度不对称现象的主要原因密度。因为变化的积累的效果,集体密度的年度不对称现象在垂直方向在中立温度和规模高度与高度增加。 | MA RuiPing XU JiYao WANG WenBin CHEN GuangMing YUAN Wei LEI JiuHou Alan G BURNS JIANG GuoYing | 2015 | Science China Earth Sciences2015，58，4： | 1 |
| 19 | Impact of conditioning regimen on peripheral blood hematopoietic cell transplant显示文摘AIM To investigate infused hematopoietic cell doses and their interaction with conditioning regimen intensity +/-total body irradiation(TBI) on outcomes after peripheral blood hematopoietic cell transplant(PBHCT).METHODS Our retrospective cohort included 247 patients receiving a first, T-replete, human leukocyte antigen-matched allogeneic PBHCT and treated between 2001 and2012. Correlations were calculated using the Pearson product-moment correlation coefficient. Overall survival and progression free survival curves were generated using the Kaplan-Meier method and compared using the log-rank test.RESULTS Neutrophil engraftment was significantly faster after reduced intensity TBI based conditioning [reduced intensity conditioning(RIC) + TBI] and > 4 × 10~6 CD34+cells/kg infused. A higher total nucleated cell dose led to a higher incidence of grade II-IV acute graft-versus-host disease in the myeloablative + TBI regimen group(P = 0.03), but no significant difference in grade III-IV graft-versus-host disease. A higher total nucleated cell dose was also associated with increased incidence of moderate/severe chronic graft-versus-host disease, regardless ofconditioning regimen. Overall and progression-free survival were significantly better in patients with a RIC + TBI regimen and total nucleated cell dose > 8 ×10~8/kg(3 years, overall survival: 70% vs 38%, P = 0.02, 3 years, progression free survival: 64% vs 38%, P = 0.02).CONCLUSION TBI and conditioning intensity may alter the relationship between infused cell doses and outcomes after PBHCT. Immune cell subsets may predict improved survival after unmanipulated PBHCT. | Michael Burns Anurag K Singh Carrie C Hoefer Yali Zhang Paul K Wallace George L Chen Alexis Platek Timothy B Winslow Austin J Iovoli Christopher Choi Maureen Ross Philip L McCarthy Theresa Hahn | 2019 | World Journal of Clinical Oncology2019，10，2： | 1 |
| 20 | Genetic and physiologic analysis of the groE operon and role of the HrcA repressor in stress gene regulation and acid tolerance in Streptococcus mutans显示文摘 | Lemos JA Chen YY Burne RA | 2001 | J Bacteriol2001，183，20： | 1 |
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