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| 1 | MYC2, MYC3, and MYC4 function additively in wounding-induced jasmonic acid biosynthesis and catabolism显示文摘Jasmonic acid(JA)plays a critical role in plant defenses against insects and necrotrophic fungi.Wounding or lepidopteran insect feeding rapidly induces a burst of JA in plants,which usually reaches peak values within 1 to 2 h.The induced JA is converted to JA-Ile and perceived by the COI1-JAZ co-receptor,leading to activation of the transcription factors MYC2 and its homologs,which further induce JA-responsive genes.Although much is known about JA biosynthesis and catabolism enzymes and JA signaling,how JA biosynthesis and catabolism are regulated remain unclear.Here,we show that in Arabidopsis thaliana MYC2 functions additively with MYC3 and MYC4 to regulate wounding-induced JA accumulation by directly binding to the promoters of genes function in JA biosynthesis and catabolism to promote their transcription.MYC2 also controls the transcription of JAV1 and JAM1,which are key factors controlling JA biosynthesis and catabolism,respectively.In addition,we also found that MYC2 could bind to the MYC2 promoter and self-inhibit its own expression.This work illustrates the central role of MYC2/3/4 in controlling wounding-induced JA accumulation by regulating the transcription of genes involved in JA biosynthesis and catabolism. | Cuiping Zhang Yunting Lei Chengkai Lu Lei Wang Jianqiang Wu | 2020 | Journal of Integrative Plant Biology2020,62,8: | 6 |
| 2 | ZnCo2O4/ZnO induced lithium deposition in multi-scaled carbon/nickel frameworks for dendrite-free lithium metal anode显示文摘Lithium metal attracts growing attention as an ideal anode candidate for next generation lithium battery systems owing to its high capacity,low density,and low working potential.However,the volume expansion of the bulk and dendrite growth on the surface of lithium anode limits its practical application.Herein,we fabricate a composite lithium host featuring both multiple scaled structure and lithiophilic property to address obstacles at both aspects of bulk and surface simultaneously.In which,the multiple scaled structure provides void space to accommodate lithium volume change while zinc and cobalt oxides sites derived from Zeolitic Imidazolate Frameworks can react with lithium and form a stable solid electrolyte interphase,leading to a stable cycling of lithium symmetrical cell for more than 500 cycles with voltage hysteresis of only 88 mV at 2 mAcm^-2 and 5 mAh cm^-2.Moreover,full cells paired with LiFePO4 cathode can realize 500 cycles with 99.2%capacity retention,showing great potential for practical applications.The excellent electrochemical performance of the composite lithium anode proves the effectiveness of our anode design with multiple scaled structure and lithiophilic feature,which can be also expanded to other metal anodes for batteries. | Kai Wu Binglu Zhao Chengkai Yang Qian Wang Wen Liu Henghui Zhou | 2020 | Journal of Energy Chemistry2020,29,4: | 4 |
| 3 | A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication显示文摘Despite the recent advances in artificial tissue and organ engineering,how to generate large size viable and functional complex organs still remains as a grand challenge for regenerative medicine.Three-dimensional bioprinting has demonstrated its advantages as one of the major methods in fabricating simple tissues,yet it still faces difficulties to generate vasculatures and preserve cell functions in complex organ production.Here,we overcome the limitations of conventional bioprinting systems by converting a six degree-of-freedom robotic arm into a bioprinter,therefore enables cell printing on 3D complex-shaped vascular scaffolds from all directions.We also developed an oil bath-based cell printing method to better preserve cell natural functions after printing.Together with a self-designed bioreactor and a repeated print-and-culture strategy,our bioprinting system is capable to generate vascularized,contractible,and long-term survived cardiac tissues.Such bioprinting strategy mimics the in vivo organ development process and presents a promising solution for in vitro fabrication of complex organs. | Zeyu Zhang Chenming Wu Chengkai Dai Qingqing Shi Guoxin Fang Dongfang Xie Xiangjie Zhao Yong-Jin Liu Charlie CLWang Xiu-Jie Wang | 2022 | Bioactive Materials2022,7,12: | 4 |
| 4 | Elevated CO2 differentially affects tobacco and rice defense against lepidopteran larvae via the jasmonic acid signaling pathway显示文摘Atmospheric CO_2 levels are rapidly increasing due to human activities. However, the effects of elevated CO_2(ECO_2) on plant defense against insects and the underlying mechanisms remain poorly understood. Here we show that ECO_2 increased the photosynthetic rates and the biomass of tobacco and rice plants, and the chewing lepidopteran insects Spodoptera litura and Mythimna separata gained less and more mass on tobacco and rice plants, respectively. Consistently, under ECO_2, the levels of jasmonic acid(JA), the main phytohormone controlling plant defense against these lepidopteran insects, as well as the main defense-related metabolites, were increased and decreased in insectdamaged tobacco and rice plants. Importantly, bioassaysand quantification of defense-related metabolites in tobacco and rice silenced in JA biosynthesis and perception indicate that ECO_2 changes plant resistance mainly by affecting the JA pathway. We further demonstrate that the defensive metabolites, but not total N or protein, are the main factors contributing to the altered defense levels under ECO_2. This study illustrates that ECO_2 changes the interplay between plants and insects, and we propose that crops should be studied for their resistance to the major pests under ECO_2 to predict the impact of ECO_2 on future agroecosystems. | Chengkai Lu Jinfeng qi Christian Hettenhausen Yunting Lei Jingxiong Zhang Mou Zhang Cuiping Zhang Juan Song Jing Li Guoyan Cao Saif ul Malook Jianqiang Wu | 2018 | Journal of Integrative Plant Biology2018,60,5: | 3 |
| 5 | Interwoven nickel(II)-dimethylglyoxime nanowires in 3D nickel foam for dendrite-free lithium deposition显示文摘Metal skeletons,such as Nickel Foam(NF) has attracted worldwide interests as stable host for lithium metal anode because of its high stability,large specific surface area and high conductivity.However,most metal skeletons have lithophobic surface and uneven current distribution that result in sporadic lithium nucleation and uncontrolled dendrites growth.Herein,we describe a sequential immersing strategy to generate interwoven Nickel(Ⅱ)-dimethylglyoxime(Ni-DMG) nanowires at NF to obtain composite skeleton(NDNF),which can be used as an stable host for Li metal storage.The Ni-DMG has proved effective to realize uniform lithium nucleation and dendrite-free lithium deposition.Combing with the three dimensional(3 D) hierarchical porous structure,the composite host shows a significantly improved coulombic efficiency(CE) than pristine commercial nickel foam.Moreover,the corresponding Li‖Li symmetrical cells can run more than 700 h with low voltage hysteresis 22 mV at 1.0 mA/cm^(2),and Li@NDNF‖LiFePO;full-cell exhibits a high capacity retention of 82.03% at 1.0 C during 630 cycles.These results proved the effectiveness of metal-organic complexes in governing Li metal growth and can be employed as a new strategy for dendrite-free Li metal anode and safe Li metal batteries(LMBs). | Tianyi Zhou Yanlu Mu Jianyang Wu Bing Zhong Chengkai Yang Qian Wang Wen Liu Henghui Zhou Peng Jiang | 2022 | Chinese Chemical Letters2022,33,4: | 1 |
| 6 | Fault di agnosis for internal combustion engines using intake mani fold pressure and artificial neural network显示文摘 | WU Jianda HUANG Chengkai CHANG Yowei | 2010 | Expert Sys terns with Applications2010,37,2: | 1 |
| 7 | An Engine Fault Diagnosis System Using Intake Manifold Pressure Signal and Wigner-Ville Distribution Technique 显示文摘 | Wu Jianda Huang Chengkai | 2011 | Expert Systems with Applications2011,38,1: | 1 |
| 8 | Multi-channel laser interferometer based on automatic frequency stabilization system for improving coordinate measurement accuracy显示文摘A multi-channel laser interferometer(MCLI)is proposed to improve the coordinate measurement accuracy.A 780 nm external cavity laser is locked on the D2 line of ^(87)Rb atom by polarization spectroscopy,and a high frequency stabilized laser source is obtained with a linewidth of 385.8 k Hz at root mean square(RMS).The interferometers share the stabilized source and individually install on 4 axes of a coordinate measuring system.As a result,the measurement uncertainty is reduced from 1.2μm to 0.2μm within the dynamic measurement range of 1.0 m.The MCLI is adept at integrate and flexible installation,which caters to various applications on precision measurement. | PANG Chengkai ZHANG Qiongqiong ZHANG Hongqiao HUANG Haiyan DENG Zejiang WU Guang | 2022 | Optoelectronics Letters2022,18,10: | 0 |
| 9 | A 16-QAM 45-Gbps 7-m Wireless Link Using InP HEMT LNA and GaAs SBD Mixers at 220-GHz-Band显示文摘This paper presents a 220-GHz-band 7-m wireless link with a 45-Gbps transmission data rate by using 16 quadrature amplitude modulation(16-QAM).Super-heterodyne transceiver modules are developed for transmission and reception of the modulated signals,which consist of a Schottky barrier diodes(SBD)based sub-harmonic mixer(SHM),an InP HEMT low noise amplifier(LNA),a waveguide band-pass filter(BPF),and a 108-GHz local oscillator(LO)multiplier chain.The transmitter features a peak transmit power of 1.41 dBm,and the IF frequency varies from 5 GHz to 20 GHz.Besides,the receiver features a conversion gain of 9.3 dB in average and a noise temperature of 3052.8 K.The measured results indicate that the transceiver modules enable data transmission of a 45-Gbps 16-QAM signal with Signal-Noise-Ratio(SNR)from 11.59 dB to 15.36 dB in a 7-m line-of-sight channel. | Yukun Li Yong Zhang Chengkai Wu Jianhan Cui Huali Zhu Bo Yan | 2021 | China Communications2021,18,5: | 0 |
| 10 | Leap of Li Metal Anodes from Coin Cells to Pouch Cells:Challenges and Progress显示文摘Li metal anodes have attracted tremendous attention in the last decade because of their high theoretical capacities and low electrochemical potentials.However,until now,there has only been limited success in improving the interfacial and structural stabilities and in realizing the highly controllable and large-scale fabrication of this emerging material;these limitations have posed great obstacles to further performing fundamental and applied studies in Li metal anodes.In this review,we focus on summarizing the existing challenges of Li metal anodes based on the leap from coin cells to pouch cells and on outlining typical methods for designing Li metal anodes on demand;we controllably engineer their surface protection layers and structure sizes by encapsulating structured Li metal inside a variety of synthetic protection layers.We aim to provide a comprehensive understanding and serve as a strategic guide for designing and fabricating practicable Li metal anodes for use in pouch batteries.Challenges and opportunities regarding this burgeoning field are critically evaluated at the end of this review. | Qian Wang Tiantian Lu Yuanbin Xiao Jianyang Wu Lixiang Guan Lifeng Hou Huayun Du Huan Wei Xiaoda Liu Chengkai Yang Yinghui Wei Henghui Zhou Yan Yu | 2023 | Electrochemical Energy Reviews2023,6,1: | 0 |
| 11 | Generation of microfluidic gradients and their effects on cells behaviours显示文摘Introduction The concept of“gradients”has been widely demonstrated and applicated in biology.For example,concentration gradients and potential gradients in the body can regulate the homeostasis as well as the balance of physiological environment;oxygen gradients play a vital role in cellular gene expression and migration. | Qiangqiang Tang Xiran Yang Chengkai Xuan Kai Wu Chen Lai Xuetao Shi | 2020 | Bio-Design and Manufacturing2020,3,4: | 0 |
| 12 | A sandwiched patch toward leakage-free and anti-postoperative tissue adhesion sealing of intestinal injuries显示文摘Ideal repair of intestinal injury requires a combination of leakage-free sealing and postoperative antiadhesion.However,neither conventional hand-sewn closures nor existing bioglues/patches can achieve such a combination.To this end,we develop a sandwiched patch composed of an inner adhesive and an outer antiadhesive layer that are topologically linked together through a reinforced interlayer.The inner adhesive layer tightly and instantly adheres to the wound sites via-NHS chemistry;the outer antiadhesive layer can inhibit cell and protein fouling based on the zwitterion structure;and the interlayer enhances the bulk resilience of the patch under excessive deformation.This complementary trilayer patch(TLP)possesses a unique combination of instant wet adhesion,high mechanical strength,and biological inertness.Both rat and pig models demonstrate that the sandwiched TLP can effectively seal intestinal injuries and inhibit undesired postoperative tissue adhesion.The study provides valuable insight into the design of multifunctional bioadhesives to enhance the treatment efficacy of intestinal injuries. | Wei Yang Chengkai Xuan Xuemin Liu Qiang Zhang Kai Wu Liming Bian Xuetao Shi | 2023 | Bioactive Materials2023,,6: | 0 |