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| 1 | Identification of biomarkers for hepatocellular carcinoma by semiquantitative immunocytochemistry显示文摘AIM:To investigate the expression of key biomarkers in hepatoma cell lines,tumor cells from patients’blood samples,and tumor tissues.METHODS:We performed the biomarker tests in two steps.First,cells plated on coverslips were used to assess biomarkers,and fluorescence intensities were calculated using the NIH Image J software.The measured values were analyzed using the SPSS19.0 software to make comparisons among eight cell lines.Second,eighty-four individual samples were used to assess the biomarkers’expression.Negative enrichment of the blood samples was performed,and karyocytes were isolated and dropped onto pretreated glass slides for further analysis by immunofluorescence staining.Fluorescence intensities were compared among hepatocellular carcinoma(HCC)patients,chronic HBV-infected patients,and healthy controls following methods similar to those used for cell lines.The relationships between the expression of biomarkers and clinical pathological parameters were analyzed by Spearman rank correlation tests.In addition,we studied the distinct biomarkers’expression with three-dimensional laser confocal microscopy reconstructions,and Kaplan-Meier survival analysis was performed to understand the clinical significance of these biomarkers.RESULTS:Microscopic examination and fluorescence intensity calculations indicated that cytokeratin 8/18/19(CK)expression was significantly higher in six of the seven HCC cell lines examined than in the control cells,and the expression levels of asialoglycoprotein receptor(ASGPR)and glypican-3(GPC3)were higher in all seven HCC cell lines than in the control.Cells obtained from HCC patients’blood samples also displayed significantly higher expression levels of ASGPR,GPC3,and CK than cells from chronic HBV-infected patients or healthy controls;these proteins may be valuable surface biomarkers for identifying HCC circulating tumor cells isolated and enriched from the blood samples.The stem cell-like and epithelial-mesenchymal transition-related biomarkers could be detected on the karyocyte slides.ASGPR and GPC3 were expressed at high levels,and thus three-dimensional reconstructions were used to observe their expression in detail.This analysis indicated that GPC3 was localized in the cytoplasm and membrane,but that ASGPR had a polar localization.Survival analyses showed that expression of GPC3 and ASGPR is associated with a patient’s overall survival(OS).CONCLUSION:ASGPR,GPC3,and CK may be valuable HCC biomarkers for CTC detection;the expression of ASGPR and GPC3 might be helpful for understanding patients’OS. | Hong Mu Kai-Xuan Lin Hong Zhao Shu Xing Cong Li Fang Liu Hai-Zhen Lu Ze Zhang Yu-Lin Sun Xi-Yun Yan Jian-Qiang Cai Xiao-Hang Zhao | 2014 | World Journal of Gastroenterology2014，20，19： | 8 |
| 2 | Meiofauna Distribution in Intertidal Sandy Beaches Along China Shoreline(18?–40?N)显示文摘In this study,the distribution pattern of meiofauna from nine sandy beaches at six latitudinal gradients along Chinese coast between 18 and 40?N was studied on their meiofauna abundance to examine the effect of latitudinal gradients.In general,meiofauna abundance was lower in four subtropical beaches in Xiamen(24?N) and Zhoushan(29?N) than that in other beaches.Meiofauna abundance differed little between tropical and temperate beaches.The taxonomic structure of meiofauna showed a dominance of nematode in colder area.The relative composition of turbellarians and polychaetes increased in warmer area.In addition to latitudinal gradient,salinity,oxygenation,sediment grain size affect also the meiofauna latitudinal distribution.As for horizontal distribution,the highest meiofauna abundance was found in low tidal zone at tropical beaches,and in middle tidal zone at temperate beaches.The horizontal distribution of meiofauna was controlled by both physical and biotic factors including feeding and anthropogenic activities.Although meiofauna abundance exhibited a horizontal difference,the composition of meiofaunal main taxa was unanimous horizontally at all beaches at the same sampling latitude. | HUA Er ZHANG Zhinan ZHOU Hong MU Fanghong LI Jia ZHANG Ting CONG Bingqing LIU Xiaoshou | 2016 | Journal of Ocean University of China2016，15，1： | 7 |
| 3 | Gastroesophageal Varices (Bleeding) and Splenomegaly: The Initial Manifestations of Some Pancreatic Body and Tail Carcinoma显示文摘INTRODUCTION The most common symptoms of the pancreatic body and tail carcinoma are epigastric pain,asthenia and back pain.However,these symptoms are nonspecific,which as a consequence leads to late diagnosis as well as a low resection rate between 10% and 30%,with a 5 years overall survival rate below 10%.[1] That how to increase early diagnosis of the pancreatic body and tail carcinoma has been a difficult problem since then.Sometimes the pancreatic body and tail carcinoma can induce splenic vein occlusion,leading to pancreatic sinistral portal hypertension (PSPH).PSPH is defined as a pathological condition of splanchnic venous hypertension localized within the left-sided gastrosplenic region in pancreatic diseases. | Xiao-Bin Li Luo Zhao Quan Liao Qiang Xu Tai-Ping Zhang Lin Cong Bing Mu | 2015 | Chinese Medical Journal2015，，4： | 3 |
| 4 | Microwave Synthesized In2S3@CNTs with Excellent Properties in Lithium-Ion Battery and Electromagnetic Wave Absorption显示文摘 | Cong Wang Congpu Mu Jianyong Xiang Bochong Wang Can Zhang Jiefang Song Fusheng Wen | 2018 | Chinese Journal of Chemistry2018，36，2： | 3 |
| 5 | SC-IrO_2 NR-carbon hybrid:A catalyst with high electrochemical stability for oxygen reduction显示文摘The enhanced electrochemical stability of the synthesized hybrid catalyst has been demonstrated by the introduction of the synergistic effect between carbon powder additive and the prepared catalyst.Single crystal IrO 2 nanorod (SC-IrO 2 NR) catalyst was prepared by a sol-gel method.The structure and performance of the catalyst sample were characterized by X-ray diffraction spectroscopy (XRD),scanning electron microscope (SEM),transmission electron microscope (TEM),rotating disk electrode (RDE) and cyclic voltammetry (CV) measurements.XRD patterns and TEM images indicate that the catalyst sample has a rutile IrO 2 single crystal nanorod structure.The onset potential for oxygen reduction reaction (ORR) of the SC-IrO 2 NR-carbon hybrid catalyst specimen is 0.75 V (vs.RHE) in RDE measurement.CV and RDE test results show that the SC-IrO 2 NR-carbon hybrid catalyst has a better electrochemical stability in comparison with the commercial Pt/C catalyst,with attenuation ratios of 17.67% and 44.60% for the SC-IrO 2 NR-carbon hybrid catalyst and the commercial Pt/C catalyst after 1500 cycles,respectively.Therefore,in terms of stability,the SC-IrO 2 NR-carbon hybrid catalyst has a promising potential in the application of the proton exchange membrane fuel cell. | WANG GuangJin CHENG Feng YU Yi LIANG Cong XU Tian PAN Mu | 2013 | Science China Chemistry2013，56，1： | 3 |
| 6 | Probabilistic ecological risk assessment of cadmium in the Bohai Sea using native saltwater species显示文摘Predicted no-effect concentration(PNEC) is often used in ecological risk assessment to determine low-risk concentrations for chemicals. In the present study, the chronic data from native saltwater species were used to calculated PNEC values using four methods: log-normal distribution(ETX 2.0), log-triangle distribution(US EPA's water quality criteria procedure), burr III distribution(Burrli OZ) and traditional assessment factor(AF). The PNECs that were calculated using four methods ranged from 0.08 μg/L to 1.8 μg/L. Three of the SSD-derived PNECs range from 0.94 to 1.8 μg/L, about a factor of two apart. To demonstrate the use of SSD-based PNEC values and comprehensively estimate the regional ecological risk for cadmium in surface water of the Bohai Sea, in the Liaodong Bay, Bohai Bay, and Laizhou Bay, China, the dissolved cadmium concentrations were measured and obtained 753 valid data covering 190 stations from July 2006 to November 2007. Based on three ecological risk assessment approaches, namely hazard quotient(HQ), probabilistic risk quotient and joint probability curve(JPC), the potential ecological risk of cadmium in surface water of the Liaodong Bay, Bohai Bay, and Laizahou Bay were estimated. Overall, the ecological risk of cadmium to aquatic ecosystem in the whole Bohai Sea was at acceptable ecological risk level, the order of ecological risk was Liaodong Bay>Bohai Bay>Laizhou Bay. However, more concerns should be paid to aquatic ecological risk in the Liaodong Bay which is the home of many steel, metallurgy and petrochemical industrial in China. | MU Jingli WANG Juying WANG Ying CONG Yi ZHANG Zhifeng | 2014 | Acta Oceanologica Sinica2014，33，12： | 2 |
| 7 | Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population:Findings from the China Cardiometabolic Disease and Cancer Cohort(4C)Study显示文摘Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women. | ZHU Yuan Yue ZHENG Rui Zhi WANG Gui Xia CHEN Li SHI Li Xin SU Qing XU Min XU Yu CHEN Yu Hong YU Xue Feng YAN Li WANG Tian Ge ZHAO Zhi Yun QIN Gui Jun WAN Qin CHEN Gang GAO Zheng Nan SHEN Fei Xia LUO Zuo Jie QIN Ying Fen HUO Ya Nan LI Qiang YE Zhen ZHANG Yin Fei LIU Chao WANG You Min WU Sheng Li YANG Tao DENG Hua Cong ZHAO Jia Jun CHEN Lu Lu MU Yi Ming TANG Xu Lei HU Ru Ying WANG Wei Qing NING Guang LI Mian LU Jie Li BI Yu Fang | 2021 | Biomedical and Environmental Sciences2021，34，1： | 2 |
| 8 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 9 | Inhibition of Newcastle disease virus replication by RNA interference targeting the matrix protein gene in chicken embryo fibroblasts显示文摘 | Yin R Ding Z Liu X Mu L Cong Y Stoeger T | 2010 | J Virol Methods2010，167，1： | 1 |
| 10 | ITO/Cu multilayer electrodes for high-brightness electrochromic displays显示文摘Fabry-Perot(F-P)nanocavity-type electrochromic devices with multicolor tunability have attracted significant interest in the past two years for their potential uses in a wide variety of applications,such as electronic display,military camouflage,and dynamic decoration.However,challenges such as insufficient brightness,lengthy switching times,and poor cycling stability have yet to be overcome.Herein,we demonstrate electrochromic electrodes based on ITO/Cu as both the current collector and the reflective layer,with WO_(3 )as the electrochromic material,forming a unique three-layered structure.The constituted WO_(3 )/ITO/Cu films present a high brightness value of about 84%before coloration,and a decent brightness value of 48%after coloration at−0.8V.Moreover,a fast switching time between different coloration states(tc=2.2s;tb=1s)is recorded,attributed to the extremely low sheet resistivity of the ITO/Cu current collector.The films also reveal excellent electrochemical cycling stability across 2000 cycles. | Xueqing Tang Zishou Hu Zhen Wang Jian Chen Xinyang Mu Ge Song Peiyan Sun Zhengji Wen Jiaming Hao Shan Cong Zhigang Zhao | 2022 | eScience2022，2，6： | 1 |
| 11 | Ecohydrological evolution model on riparian vegetation in hyper-arid regions and its validation in the lower reach of Tarim River 显示文摘 | LIU Dengfeng TIAN Fuqiang HU Heping LIN Mu CONG Zhentao | 2012 | Hydrological Processes2012，26，13： | 1 |
| 12 | Inhibition of Newcastle disease virus replication by RNA interference targeting the matrix protein gene in chicken embryo fibroblasts显示文摘 | Renfu Yin Zhuang Ding Xinxin Liu Lianzhi Mu Yanlong Cong Tobias Stoeger | 2010 | Journal of Virological Methods2010，，1： | 1 |
| 13 | Antigenic variation between Newcastle disease viruses of goose and chicken origin显示文摘 | Zhi-jie Li Yang Li Shuang Chang Zhuang Ding Lian-zhi Mu Yan-long Cong | 2010 | Archives of Virology2010，，4： | 1 |
| 14 | Theoretical study of n-budding opening-up vesicle based on the spontaneous curvature model显示文摘N-budding lipid vesicles with single holes were analyzed theoretically by taking into account the line tension of membrane with a hole and the bending energy of membranes based on the Spontaneous-Curvature (SC) model. We solved numerically the Euler-Lagrange equation and the boundary conditions holding on the membrane edge to obtain axisymmetric vesicle shapes, which minimize the total energy. The numerical results show that the size of the opening-up vesicle is larger when the line tension is lower. In other words, additive protein molecules reduce the line tension of lipid membranes, such as talin dose. | KANG WenBin ZHANG ShaoGuang WANG Ying MU YaRong HUANG Cong | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，12： | 1 |
| 15 | A Sterically Hindered Zirconocene Complex (1,2-Ph_2C_5H_3)_2ZrCl_2:Synthesis,Structure and Properties as Olefin Polymerization Catalyst显示文摘 | Fan ZHANG Ying MU Yue Tao ZHANG Cong Xi CHEN Hui Min ZHAI Han HONG(Department of Chemistry, Jilin University, Changchun 130023Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080) | 2000 | Chinese Chemical Letters2000，11，7： | 1 |
| 16 | Inhibition of Newcastle disease virus replication by RNA interference targeting the matrix protein gene in chicken embryo fibroblasts显示文摘 | Renfu Yin Zhuang Ding Xinxin Liu Lianzhi Mu Yanlong Cong Tobias Stoeger | 2010 | Journal of Virological Methods2010，，1： | 1 |
| 17 | Physiological and biochemical mechanism of allelopathy of secalonic acid F on higher plants显示文摘 | ZENG R S LUO S M YUE H S MU B S CONG Y T | 2001 | Agronomy Journal2001，93，： | 1 |
| 18 | The osteogenic niche-targeted arsenic nanoparticles prevent colonization of disseminated breast tumor cells in the bone显示文摘Up to 70% of patients with late-stage breast cancer have bone metastasis. Current treatment regimens for breast cancer bone metastasis are palliative with no therapeutic cure. Disseminated tumor cells(DTCs) colonize inside the osteogenic niches in the early stage of bone metastasis. Drug delivery into osteogenic niches to inhibit DTC colonization can prevent bone metastasis from entering its late stage and therefore cure bone metastasis. Here, we constructed a 50% DSS6 peptide conjugated nanoparticle to target the osteogenic niche. The osteogenic niche was always located at the endosteum with immature hydroxyapatite. Arsenic-manganese nanocrystals(around 14 nm) were loaded in osteogenic niche-targeted PEG-PLGA nanoparticles with an acidic environment-triggered arsenic release. Arsenic formulations greatly reduced 4 T1 cell adhesion to mesenchymal stem cells(MSCs)/preosteoblasts(pre-OBs) and osteogenic differentiation of osteoblastic cells. Arsenic formulations also prevented tumor cell colonization and dormancy via altering the direct interaction between 4 T1 cells and MSCs/pre-OBs.The chemotactic migration of 4 T1 cells toward osteogenic cells was blocked by arsenic in mimic 3 D osteogenic niche. Systemic administration of osteogenic niche-targeted arsenic nanoparticles significantly extended the survival of mice with 4 T1 syngeneic bone metastasis. Our findings provide an effective approach for osteogenic niche-specific drug delivery and suggest that bone metastasis can be effectively inhibited by blockage of tumor cell colonization in the bone microenvironment. | Cong Liu Anzhi Hu Huijuan Chen Jing Liang Mancang Gu Yang Xiong Chao-Feng Mu | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 0 |
| 19 | Innovation and Practice of Training Mode for Professional Postgraduates of Acupuncture and Tuina Based on Artificial Intelligence显示文摘In view of the common problems of integrating artificial intelligence into the training of postgraduates in Acupuncture and Tuina major,this paper reviews the related research progress both at home and abroad.It puts forward the innovative reform paths for integrating artificial intelligence into postgraduate training mode of Acupuncture and Tuina major:construct the teaching staff of artificial intelligence graduate students;innovating artificial intelligence to promote the integration of classics and scientific research;constructing the ideological and political case base of artificial intelligence courses;implementing artificial intelligence platform blended teaching;building a domestic and foreign exchange platform for artificial intelligence.Through practical research in teaching,it has achieved good teaching results and played a good demonstration,leading and radiation role in similar majors in China. | Mingjun LIU Xiaochao GANG Zhengri CONG Junhao HU Jianfeng LIANG Chongwen ZHONG Xinyi YUAN Bing DAI Yuzhe ZHANG Lijie LI Tianyi MU Yiran HAN Chaochao HUA | 2024 | Asian Agricultural Research2024，16，3： | 0 |
| 20 | Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.显示文摘Sesame Fusarium wilt(SFW),caused by Fusarium oxysporum f.sp.sesami(Fos),is one of the most devastating diseases affecting sesame cultivation.Deciphering the genetic control of SFW resistance is pivotal for effective disease management in sesame.An inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indicated that resistance was conferred by a single dominant allele.The target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked segregant analysis.Fine genetic mapping further narrowed the interval between 21,350 and 21,401 kb.The locus Sindi_0812400 was identified as the SFW resistance gene and officially designated SiRLK1.This gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion protein.Sequence analysis revealed that a high proportion(49.44%)of variants within the locus was located within the kinase domainⅢ,and several of which were evidently associated with the diversity in SFW response,indicating the critical role of kinase domainⅢin expression of disease resistance.These findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame. | Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li Qiuzhen Tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao | 2024 | The Crop Journal2024，12，1： | 0 |
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