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| 1 | Characterization of NDM-1-producing carbapenemase in Acinetobacter spp.and E.coli isolates from diseased pigs显示文摘In recent years,the mobile metallo-β-lactamase(MBL)genes have been found to correspond to one of the most important resistance characters identified in Gramnegative bacteria,severely affecting clinical chemotherapy and threatening public health.The prevalence of mobile MBL genes and their flanking regions in Gram-negative bacteria from diseased pigs in China was investigated.A total of 334 lung samples from diseased pigs were screened for Gram-negative bacteria classified as non-susceptible to meropenem(MIC≥4mg·L^(–1)).Six isolates,including three Escherichia coli,two Acinetobacter baumanii and one A.calcoaeticus,exhibitedMBL production and carried the blaNDM-1 gene.S1-PFGE and Southern blot analysis showed that the blaNDM-1 gene was located on the chromosome of one A.baumanii isolate and on plasmids of various sizes in the other five isolates.MIC testing using broth microdilution revealed that all blaNDM-1-carrying isolates and some of their transconjugants exhibited resistance to almost allβ-lactams tested.Whole genome sequencing revealed that the flanking region of the blaNDM-1 gene from all porcine isolates had high levels of similarity with the corresponding regions in human isolates.One porcine E.coli isolate carrying blaNDM-1 was typed as ST48,a common sequence type in human E.coli isolates.These results suggest the possibility of human-tofood animal transfer of blaNDM-1-producing E.coli,highlighting the need for surveillance of carbapenemase producers among bacteria from food animals.In addition,the prudent use of antimicrobial agents to decrease the opportunities for co-selection of carbapenemase genes in food animals is also urgently needed. | Rongmin ZHANG Yang WANG Zhihai LIU Jiyun LI Wenjuan YIN Lei LEI Congming WU Jianzhong SHEN | 2015 | Frontiers of Agricultural Science and Engineering2015，2，3： | 2 |
| 2 | Molecular Epidemiology of Klebsiella pneumoniae from Clinical Bovine Mastitis in Northern Area of China,2018–2019显示文摘Klebsiella pneumoniae(K.pneumonia,KpⅠ)is a predominate inducement of bovine mastitis,which is associated with high mortality and milk yield reduction.However,data is lacking on the molecular characteristics of bovine K.pneumoniae,limiting the risk assessment of its transmission through the food chain.Herein,we investigated the prevalence of K.pneumoniae in 6301 clinical mastitis(CM)milk samples from dairy cattle in northern area of China.In total,183 K.pneumoniae isolates were recovered,with detection rates of 3.0% and 2.8% in 2018 and 2019,respectively.Like human clinical K.pneumoniae,all CM K.pneumoniae isolates belonged to one of three phylogroups:KpⅠ(n=143),Klebsiella.quasipneumoniae subsp.similipneumoniae(KpⅡ-B)(n=37),and Klebsiella variicola(KpⅢ)(n=3).We detected the extendedspectrum β-lactamase-encoding genes bla_(SHV-2a),blac_(CTX-M-14),and bla_(CTX-M-15),as well as clpC,lpfA,lacI,lacZ,lacY,and the fecABDEIR operon in the KpⅠ isolates,which may contribute to their pathogenicity and host adaptability in cows.The high prevalence of KpⅠ in dairy farms may be problematic,as it showed relatively higher rates of antibiotic resistance and virulence gene carriage than the KpⅡ-B and KpⅢ isolates.Furthermore,we observed distinct differences in population structure between CM-and human infection-associated KpⅠ isolates,with the genes associated with invasive infection in humans rarely being observed in bovine isolates,indicating that few CM-associated K.pneumoniae isolates pose a threat to human health.Nevertheless,bovine KpⅡ-B isolates shared a high level of nucleotide sequence identity with isolates from human infections and frequently carried the nitrogen-fixation gene nif,suggesting an association between KpⅡ-B isolates from cattle and humans,and plant-derived bacteria. | Shikai Song Wenjuan He Dawei Yang Manar Benmouffok Yao Wang Jiyun Li Chengtao Sun Xiangbin Song Shizhen Ma Chang Cai Shuangyang Ding Congming Wu Zhangqi Shen Yang Wang | 2022 | Engineering2022，8，3： | 1 |
| 3 | Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and veterinary staff in China显示文摘 | Wanjiang Zhang Zhihui Hao Yang Wang Xingyuan Cao Catherine M. Logue Bing Wang Jing Yang Jianzhong Shen Congming Wu | 2011 | The Veterinary Journal2011，，2： | 1 |
| 4 | A Psb27 homologue in Arabidopsis thaliana is required for efficient repair of photodamaged photosystem II显示文摘 | Hua Chen Dongyuan Zhang Jinkui Guo Hao Wu Meifang Jin Qingtao Lu Congming Lu Lixin Zhang | 2006 | Plant Molecular Biology (-)2006，，4： | 1 |
| 5 | Prevalence and characterization of Salmonella species isolated from pigs, ducks and chickens in Sichuan Province, China显示文摘 | Ruichao Li Jing Lai Yang Wang Shuliang Liu Yun Li Kunyao Liu Jianzhong Shen Congming Wu | 2013 | International Journal of Food Microbiology2013，，1： | 1 |
| 6 | Prevalence and characterization of methicillin susceptible Staphylococcus aureus ST398 isolates from retail foods显示文摘 | Guanghui Li Congming Wu Xin Wang | 2015 | International Journal of Food Microbiology2015，196，： | 1 |
| 7 | First Report of the Multiresistance Gene cfr in Streptococcus suis显示文摘 | Yang Wang Dexi Li Li Song Yang Liu Tao He Hebing Liu Congming Wu Stefan Schwarz Jianzhong Shen | 2013 | Antimicrobial Agents and Chemotherapy2013，，8： | 1 |
| 8 | Serotype distribution and antibiotic resistance of Salmonella infood-producing animals in Shandong province of China, 2009 and 2012 显示文摘 | JING Laia WUA Congming WU Chenbin | 2014 | Int J Food Microbiol2014，180，： | 1 |
| 9 | Tracking Campylobacter contamination along a broiler chicken production chain from the farm level to retail in China显示文摘 | Licai Ma Yang Wang Jianzhong Shen Qijing Zhang Congming Wu | 2014 | International Journal of Food Microbiology2014，，： | 1 |
| 10 | Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive Escherichia coli Strains显示文摘Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing animals.At present,the impact of livestock-associated CREC strains on human populations at the national level is unknown.Here,we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence.We also ascertain the blaNDMand blaKPCabundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin.Overall,631/29799(2.1%)clinical Escherichia coli(E.coli)isolates were identified as CREC.Multivariable analysis revealed that being male,an age below 1,an age between 13 and 18,provinces with greater chicken production,and provinces with higher pig production were associated with higher odds of CREC infection.In general,73.8%(n=45/61)of pig farms and 62.2%(n=28/45)of chicken farms had a blaNDMabundance of 1×10^(-5)to 1×10^(-3)and 1×10^(-3)to 1×10^(-2),respectively.Among all the Chinese NDM-positive E.coli(n=463)available at the National Center for Biotechnology Information(NCBI),the genomic analysis revealed that blaNDM-5and Inc X3 were the predominant carbapenemase gene-plasmid combination,while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels.All the findings suggest frequent CREC transmission between humans and animals,indicating that further discussions on the use of antibiotics in animals and humans are needed,both in China and across the globe. | Yingbo Shen Fupin Hu Yongqiang Wang Dandan Yin Lu Yang Yiqiang Chen Chunyan Xu Jiyun Li Junyao Jiang Xueyang Wang Yulin Fu Dongyan Shao Dejun Liu Tengfei Ma Chang Cai Zhangqi Shen Shaolin Wang Juan Li Rong Zhang Yuebin Ke Congming Wu Jianzhong Shen Timothy R.Walsh Yang Wang | 2022 | Engineering2022，8，8： | 0 |
| 11 | New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq显示文摘Dear Editor,Theworld had suffered pandemics of COVID-19 caused by SARS-CoV-2.At the time ofwriting,COVID-19 is still widely spreading inmany countries and regions,and by June 14,2022,totally 532,887,351 cases,including 6,307,021 deaths,were reported globally(WHO,http://gffzze7c39cdc9e894ca6skknxbwubkov96pwx.ffgz.tsg.suse.edu.cn/table).Powerful measures,such as prophylactic and therapeutic interventions against the virus,are in urgent need for COVID-19 control and prevention.The spike protein,which is expressed on the virus surface,is the key determinant of viral-host interaction(Ng et al.,2021)and mediates the virus entry. | Mengyuan Chen Shuizhen He Hualong Xiong Dongxu Zhang Shaojuan Wang Wangheng Hou Xiaomei Zhu Jin Wang Yang Huang Congming Hong Yubin Wu Ruoyao Qi Tianying Zhang Quan Yuan Tingdong Li Yixin Chen Shiyin Zhang Shengxiang Ge Jun Zhang Ningshao Xia | 2022 | Virologica Sinica2022，37，5： | 0 |
| 12 | Genomic Shift in Population Dynamics of mcr-1-positive Escherichia coli in Human Carriage显示文摘Emergence of the colistin resistance gene,mcr-1,has attracted worldwide attention.Despite the prevalence of mcr-1-positive Escherichia coli(MCRPEC)strains in human carriage showing a significant decrease between 2016 and 2019,genetic differences in MCRPEC strains remain largely unknown.We therefore conducted a comparative genomic study on MCRPEC strains from fecal samples of healthy human subjects in 2016 and 2019.We identified three major differences in MCRPEC strains between these two time points.First,the insertion sequence ISApl1 was often deleted and the percentage of mcr-1-carrying IncI2 plasmids was increased in MCRPEC strains in 2019.Second,the antibiotic resistance genes(ARGs),aac(3)-IVa and blaCTX-M-1,emerged and coexisted with mcr-1 in 2019.Third,MCRPEC strains in 2019 contained more virulence genes,resulting in an increased proportion of extraintestinal pathogenic E.coli(ExPEC)strains(36.1%)in MCRPEC strains in 2019 compared to that in 2016(10.5%),implying that these strains could occupy intestinal ecological niches by competing with other commensal bacteria.Our results suggest that despite the significant reduction in the prevalence of MCRPEC strains in humans from 2016 to 2019,MCRPEC exhibits increased resistance to other clinically important ARGs and contains more virulence genes,which may pose a potential public health threat. | Yingbo Shen Rong Zhang Dongyan Shao Lu Yang Jiayue Lu Congcong Liu Xueyang Wang Junyao Jiang Boxuan Wang Congming Wu Julian Parkhill Yang Wang Timothy R.Walsh George F.Gao Zhangqi Shen | 2022 | Genomics, Proteomics & Bioinformatics2022，20，6： | 0 |
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